pycom15g18180

) efflux antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007041
Physical Location & Seq
Reverse (-)
12892846 .. 12895907
3062 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g18180.5

Sequence Viewer

Length: 993 bp
ATGTGCTTGTGTGGAATAACAGCCTCGTTATGCGGTGGGAGGCCTTCAGAAGGGGTATTTGTTGGTGCATTTCTTTCTATGTCTTCGACAGCAGTGGTTTTGAAATTCTTGATGGAAAGAAATAGTATCAGTGCTCTTCATGGTCAAGTTGTAGTTGGCACTCTTATACTACAGGACTGTGCGGTGGGTTTGCTATTTGCTCTGCTTCCAGTTCTGGGTGGGACTTCTGGTATTCTTCAAGGAGTAATATCCATGACTAAATCGCTGTTGGTTTTGCTTATGTTTTTGGCCACCTTGTCCATATTAGCACGTACTTGTGTACCATGGTTCCTGAAGCTTATGATAAGCCTATCTTCTCAGACAAATGAACTGTATCAGTTGGCATCAGTGGCGTTCTGCTTACTAGTTGCCTGGTGTAGTGATAAGCTGGGTTTAAGCCTTGAACTGGGTTCCTTTGCGGCTGGAGTGATGATATCAACAACTGATCTTGGTCAGCATACGCTGGAGCAAGTTGAACCCATTCGCAATTTCTTTGCTGCTCTTTTTCTCGCCAGCATTGGGATGTTAATACATGTTCATTTCCTTTGGAATCATGTTGATATTTTATTAGCAGCTGTTATATTGGTAATCATTGTAAAAACTGTAGTGGTTGGTTGCGTGGTCAAGGGATTTGGATACAATAACAAGACTTCCCTTCTTGTTGGGATGTCTCTTGCACAAATCGGAGAATTTGCTTTCGTTCTTCTAAGCCGTGCTTCTAATCTTCATCTAGTTGAGGGTAAATTATATCTATTGCTTCTAGGCACAACAGCTCTCAGTCTGGTCACTACGCCATTGCTTTTCAAGATAATTCCTGCTGTTGTCCATCTTGGGGTGTTGCTGCGGTGGTTCTCCCCCGATAGCCTGAGTGAGATTGGATTTAAAGGAGATAGCTTTCGCTCAGACAGTGCTAAGCGTTTTGCTATGATGCTCCACGGATCTCATGATTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0034220 GO:0051179 GO:0051234 GO:0055085 GO:0098655
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

331

Amino Acids

35.37

Weight (kDa)

8.13

Isoelectric Point (pI)

27.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 33, 182, 458, 883
AclWI GGATC 1 cut(s) 985
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 2 cut(s) 104, 728
AcuI CTGAAG 2 cut(s) 30, 353
AfaI GTAC 2 cut(s) 313, 321
AfiI CCNNNNNNNGG 5 cut(s) 50, 215, 445, 558, 871
AflIII ACRYGT 1 cut(s) 571
AgsI TTSAA 5 cut(s) 103, 239, 443, 515, 844
AhlI ACTAGT 1 cut(s) 403
AjnI CCWGG 1 cut(s) 410
AluBI AGCT 5 cut(s) 337, 427, 614, 812, 933
AluI AGCT 5 cut(s) 337, 427, 614, 812, 933
Alw21I GWGCWC 1 cut(s) 136
Alw26I GTCTC 1 cut(s) 714
AlwI GGATC 1 cut(s) 985
AoxI GGCC 2 cut(s) 41, 288
ApeKI GCWGC 3 cut(s) 536, 611, 880
ApoI RAATTY 2 cut(s) 104, 728
Asp700I GAANNNNTTC 1 cut(s) 519
BaeI ACNNNNGTAYC 2 cut(s) 303, 336
BalI TGGCCA 1 cut(s) 290
BbsI GAAGAC 1 cut(s) 75
Bbv12I GWGCWC 1 cut(s) 136
BbvI GCAGC 3 cut(s) 523, 623, 867
BccI CCATC 2 cut(s) 106, 873
BceAI ACGGC 1 cut(s) 735
BciT130I CCWGG 1 cut(s) 412
BciVI GTATCC 1 cut(s) 668
BcoDI GTCTC 1 cut(s) 714
BcuI ACTAGT 1 cut(s) 403
BfaI CTAG 3 cut(s) 404, 770, 800
BfmI CTRYAG 2 cut(s) 170, 642
BfuI GTATCC 1 cut(s) 668
BisI GCNGC 4 cut(s) 459, 537, 612, 881
BlpI GCTNAGC 1 cut(s) 951
BlsI GCNGC 4 cut(s) 460, 538, 613, 882
Bme1390I CCNGG 1 cut(s) 412
BmiI GGNNCC 2 cut(s) 329, 451
BmrFI CCNGG 1 cut(s) 412
BmrI ACTGGG 1 cut(s) 455
BmsI GCATC 2 cut(s) 392, 957
BmuI ACTGGG 1 cut(s) 455
BpiI GAAGAC 1 cut(s) 75
BpmI CTGGAG 2 cut(s) 483, 524
Bpu1102I GCTNAGC 1 cut(s) 951
BsaAI YACGTR 1 cut(s) 311
BsaJI CCNNGG 2 cut(s) 323, 973
Bsc4I CCNNNNNNNGG 5 cut(s) 50, 215, 445, 558, 871
Bse1I ACTGG 2 cut(s) 209, 450
Bse3DI GCAATG 1 cut(s) 833
BseBI CCWGG 1 cut(s) 412
BseDI CCNNGG 2 cut(s) 323, 973
BseGI GGATG 2 cut(s) 567, 711
BseLI CCNNNNNNNGG 5 cut(s) 50, 215, 445, 558, 871
BseMI GCAATG 1 cut(s) 833
BseMII CTCAG 4 cut(s) 371, 829, 896, 954
BseNI ACTGG 2 cut(s) 209, 450
BseXI GCAGC 3 cut(s) 523, 623, 867
BseYI CCCAGC 1 cut(s) 427
BshFI GGCC 2 cut(s) 43, 290
BsiHKAI GWGCWC 1 cut(s) 136
BslFI GGGAC 1 cut(s) 235
BslI CCNNNNNNNGG 5 cut(s) 50, 215, 445, 558, 871
BsmAI GTCTC 1 cut(s) 714
BsmFI GGGAC 1 cut(s) 235
BsnI GGCC 2 cut(s) 43, 290
Bsp1286I GDGCHC 1 cut(s) 136
Bsp143I GATC 2 cut(s) 484, 977
Bsp1720I GCTNAGC 1 cut(s) 951
Bsp19I CCATGG 1 cut(s) 323
BspACI CCGC 4 cut(s) 33, 182, 458, 883
BspANI GGCC 2 cut(s) 43, 290
BspCNI CTCAG 4 cut(s) 370, 828, 897, 953
BspHI TCATGA 1 cut(s) 982
BspLI GGNNCC 2 cut(s) 329, 451
BspPI GGATC 1 cut(s) 985
BspQI GCTCTTC 1 cut(s) 141
BsrDI GCAATG 1 cut(s) 833
BsrI ACTGG 2 cut(s) 209, 450
BssECI CCNNGG 2 cut(s) 323, 973
BssMI GATC 2 cut(s) 484, 977
BssT1I CCWWGG 1 cut(s) 323
Bst2UI CCWGG 1 cut(s) 412
Bst4CI ACNGT 4 cut(s) 179, 372, 643, 947
Bst6I CTCTTC 1 cut(s) 141
BstBAI YACGTR 1 cut(s) 311
BstC8I GCNNGC 1 cut(s) 553
BstDEI CTNAG 6 cut(s) 357, 746, 815, 905, 940, 951
BstDSI CCRYGG 2 cut(s) 323, 973
BstENI CCTNNNNNAGG 1 cut(s) 48
BstF5I GGATG 2 cut(s) 567, 711
BstKTI GATC 2 cut(s) 487, 980
BstMAI GTCTC 1 cut(s) 714
BstMBI GATC 2 cut(s) 484, 977
BstMWI GCNNNNNNNGC 1 cut(s) 389
BstNI CCWGG 1 cut(s) 412
BstNSI RCATGY 1 cut(s) 575
BstSCI CCNGG 1 cut(s) 410
BstSFI CTRYAG 2 cut(s) 170, 642
BstV1I GCAGC 3 cut(s) 523, 623, 867
BstV2I GAAGAC 1 cut(s) 75
BstX2I RGATCY 1 cut(s) 977
BstYI RGATCY 1 cut(s) 977
BsuI GTATCC 1 cut(s) 668
BsuRI GGCC 2 cut(s) 43, 290
BtgI CCRYGG 2 cut(s) 323, 973
BtsCI GGATG 2 cut(s) 567, 711
BtsI GCAGTG 1 cut(s) 99
BtsIMutI CAGTG 4 cut(s) 99, 136, 393, 952
Cac8I GCNNGC 1 cut(s) 553
CciI TCATGA 1 cut(s) 982
Csp6I GTAC 2 cut(s) 312, 320
CviAII CATG 6 cut(s) 140, 253, 324, 572, 593, 983
CviQI GTAC 2 cut(s) 312, 320
DdeI CTNAG 6 cut(s) 357, 746, 815, 905, 940, 951
DpnI GATC 2 cut(s) 486, 979
DpnII GATC 2 cut(s) 484, 977
DraI TTTAAA 1 cut(s) 922
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
Eco130I CCWWGG 1 cut(s) 323
Eco147I AGGCCT 1 cut(s) 43
Eco32I GATATC 1 cut(s) 474
Eco57I CTGAAG 2 cut(s) 30, 353
EcoNI CCTNNNNNAGG 1 cut(s) 48
EcoRII CCWGG 1 cut(s) 410
EcoRV GATATC 1 cut(s) 474
EcoT14I CCWWGG 1 cut(s) 323
ErhI CCWWGG 1 cut(s) 323
FaeI CATG 6 cut(s) 143, 256, 327, 575, 596, 986
FalI AAGNNNNNCTT 4 cut(s) 337, 369, 739, 771
FaqI GGGAC 1 cut(s) 235
FatI CATG 6 cut(s) 139, 252, 323, 571, 592, 982
Fnu4HI GCNGC 4 cut(s) 459, 537, 612, 881
FokI GGATG 2 cut(s) 574, 718
Fsp4HI GCNGC 4 cut(s) 459, 537, 612, 881
FspBI CTAG 3 cut(s) 404, 770, 800
GluI GCNGC 4 cut(s) 459, 537, 612, 881
GsaI CCCAGC 1 cut(s) 431
GsuI CTGGAG 2 cut(s) 483, 524
HaeIII GGCC 2 cut(s) 43, 290
Hin1II CATG 6 cut(s) 143, 256, 327, 575, 596, 986
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 1 cut(s) 589
Hpy166II GTNNAC 1 cut(s) 320
Hpy188I TCNGA 4 cut(s) 49, 360, 725, 943
Hpy188III TCNNGA 4 cut(s) 109, 331, 844, 983
Hpy8I GTNNAC 1 cut(s) 320
HpyAV CCTTC 3 cut(s) 44, 54, 704
HpyCH4III ACNGT 4 cut(s) 179, 372, 643, 947
HpyCH4IV ACGT 1 cut(s) 310
HpyCH4V TGCA 2 cut(s) 68, 716
HpyF10VI GCNNNNNNNGC 1 cut(s) 389
HpyF3I CTNAG 6 cut(s) 357, 746, 815, 905, 940, 951
HpySE526I ACGT 1 cut(s) 310
Hsp92II CATG 6 cut(s) 143, 256, 327, 575, 596, 986
Kzo9I GATC 2 cut(s) 484, 977
LguI GCTCTTC 1 cut(s) 141
LmnI GCTCC 2 cut(s) 505, 975
Lsp1109I GCAGC 3 cut(s) 523, 623, 867
LweI GCATC 2 cut(s) 392, 957
MaeI CTAG 3 cut(s) 404, 770, 800
MaeII ACGT 1 cut(s) 310
MaeIII GTNAC 1 cut(s) 823
MalI GATC 2 cut(s) 486, 979
MboI GATC 2 cut(s) 484, 977
MboII GAAGA 6 cut(s) 75, 128, 227, 345, 734, 755
MflI RGATCY 1 cut(s) 977
MhlI GDGCHC 1 cut(s) 136
MlsI TGGCCA 1 cut(s) 290
MluCI AATT 5 cut(s) 104, 526, 728, 782, 849
MluNI TGGCCA 1 cut(s) 290
MnlI CCTC 3 cut(s) 33, 34, 769
Mox20I TGGCCA 1 cut(s) 290
MroXI GAANNNNTTC 1 cut(s) 519
MscI TGGCCA 1 cut(s) 290
MseI TTAA 3 cut(s) 434, 566, 921
MslI CAYNNNNRTG 1 cut(s) 560
Msp20I TGGCCA 1 cut(s) 290
MspA1I CMGCKG 1 cut(s) 614
MspR9I CCNGG 1 cut(s) 412
MvaI CCWGG 1 cut(s) 412
MwoI GCNNNNNNNGC 1 cut(s) 389
NcoI CCATGG 1 cut(s) 323
NdeII GATC 2 cut(s) 484, 977
NlaIII CATG 6 cut(s) 143, 256, 327, 575, 596, 986
NlaIV GGNNCC 2 cut(s) 329, 451
NmuCI GTSAC 1 cut(s) 823
NspI RCATGY 1 cut(s) 575
PagI TCATGA 1 cut(s) 982
PceI AGGCCT 1 cut(s) 43
PciI ACATGT 1 cut(s) 571
PciSI GCTCTTC 1 cut(s) 141
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 1 cut(s) 589
PkrI GCNGC 4 cut(s) 460, 538, 613, 882
Ppu21I YACGTR 1 cut(s) 311
PscI ACATGT 1 cut(s) 571
Psp6I CCWGG 1 cut(s) 410
PspFI CCCAGC 1 cut(s) 427
PspGI CCWGG 1 cut(s) 410
PspN4I GGNNCC 2 cut(s) 329, 451
PsuI RGATCY 1 cut(s) 977
PvuII CAGCTG 1 cut(s) 614
RsaI GTAC 2 cut(s) 313, 321
RsaNI GTAC 2 cut(s) 312, 320
RseI CAYNNNNRTG 1 cut(s) 560
SapI GCTCTTC 1 cut(s) 141
SaqAI TTAA 3 cut(s) 434, 566, 921
SatI GCNGC 4 cut(s) 459, 537, 612, 881
Sau3AI GATC 2 cut(s) 484, 977
ScrFI CCNGG 1 cut(s) 412
SduI GDGCHC 1 cut(s) 136
SetI ASST 7 cut(s) 296, 313, 339, 429, 616, 814, 935
SfaNI GCATC 2 cut(s) 392, 957
SfcI CTRYAG 2 cut(s) 170, 642
SmiMI CAYNNNNRTG 1 cut(s) 560
SpeI ACTAGT 1 cut(s) 403
Sse9I AATT 5 cut(s) 104, 526, 728, 782, 849
SseBI AGGCCT 1 cut(s) 43
SsiI CCGC 4 cut(s) 33, 182, 458, 883
SspMI CTAG 3 cut(s) 404, 770, 800
StuI AGGCCT 1 cut(s) 43
StyD4I CCNGG 1 cut(s) 410
StyI CCWWGG 1 cut(s) 323
TaaI ACNGT 4 cut(s) 179, 372, 643, 947
TaiI ACGT 1 cut(s) 313
TaqI TCGA 1 cut(s) 86
TasI AATT 5 cut(s) 104, 526, 728, 782, 849
TauI GCSGC 1 cut(s) 461
TfiI GAWTC 1 cut(s) 589
Tru1I TTAA 3 cut(s) 434, 566, 921
Tru9I TTAA 3 cut(s) 434, 566, 921
TscAI CASTG 4 cut(s) 99, 136, 393, 952
TseFI GTSAC 1 cut(s) 823
TseI GCWGC 3 cut(s) 536, 611, 880
Tsp45I GTSAC 1 cut(s) 823
TspDTI ATGAA 4 cut(s) 128, 381, 566, 755
TspGWI ACGGA 1 cut(s) 990
TspRI CASTG 4 cut(s) 99, 136, 393, 952
XagI CCTNNNNNAGG 1 cut(s) 48
XapI RAATTY 2 cut(s) 104, 728
XceI RCATGY 1 cut(s) 575
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 3 cut(s) 404, 770, 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.