Rorug02G0032300

) efflux antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007041
Physical Location & Seq
Reverse (-)
2554820 .. 2555179
360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0032300.1

Sequence Viewer

Length: 360 bp
ATGGCTGGCACGGGTATTGGCTCAACGGCGTTGGCGCATGGCTCTGCACGGGTGTCGCTGGGTTCCGTGGGGCTTGAGGCTCTGAAGGTGGATCGTTGGTACATGGTGGGGCGTTTACTTGGACCCAAAGCCTTGTTTCCTGGGTTCAAAGGAACAATCTCTTTAATCTGGAGGATTCGTTCGGGGCTAACGATCCAAGATGTGGGGGATCGGTTTGTGCTTCATTTCGATCGAGAAGTTGATCGGAACAAAATATTGCATGGAGGCCCATGGTTCTATAAGAATACCATGCTTGTGCTGGGAGTGTACGATGGGTTAGGACCAGTGGCGGAAGTCCCTCTAAATCTTTTGGAGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0034220 GO:0051179 GO:0051234 GO:0055085 GO:0098655
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

119

Amino Acids

12.95

Weight (kDa)

9.52

Isoelectric Point (pI)

21.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 32 - 101 2e-09 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 202
AciI CCGC 1 cut(s) 329
AclWI GGATC 3 cut(s) 99, 187, 216
AcuI CTGAAG 1 cut(s) 104
AfaI GTAC 2 cut(s) 101, 308
AfiI CCNNNNNNNGG 1 cut(s) 202
AgsI TTSAA 1 cut(s) 148
AjnI CCWGG 1 cut(s) 139
AloI GAACNNNNNNTCC 2 cut(s) 163, 195
Alw26I GTCTC 1 cut(s) 347
AlwI GGATC 3 cut(s) 99, 187, 216
AoxI GGCC 1 cut(s) 265
AspLEI GCGC 1 cut(s) 37
AspS9I GGNCC 3 cut(s) 122, 266, 320
AvaII GGWCC 2 cut(s) 122, 320
BccI CCATC 1 cut(s) 305
BceAI ACGGC 1 cut(s) 42
BcgI CGANNNNNNTGC 2 cut(s) 36, 70
BciT130I CCWGG 1 cut(s) 141
BcoDI GTCTC 1 cut(s) 347
Bme1390I CCNGG 1 cut(s) 141
Bme18I GGWCC 2 cut(s) 122, 320
BmgT120I GGNCC 3 cut(s) 122, 266, 320
BmiI GGNNCC 2 cut(s) 64, 124
BmrFI CCNGG 1 cut(s) 141
BpmI CTGGAG 1 cut(s) 190
BpuEI CTTGAG 1 cut(s) 95
BsaJI CCNNGG 3 cut(s) 66, 140, 269
BsaXI ACNNNNNCTCC 2 cut(s) 163, 193
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse1I ACTGG 1 cut(s) 323
BseBI CCWGG 1 cut(s) 141
BseDI CCNNGG 3 cut(s) 66, 140, 269
BseLI CCNNNNNNNGG 1 cut(s) 202
BseNI ACTGG 1 cut(s) 323
BseYI CCCAGC 2 cut(s) 58, 298
BsgI GTGCAG 1 cut(s) 30
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 1 cut(s) 267
BsiEI CGRYCG 1 cut(s) 232
BslFI GGGAC 1 cut(s) 320
BslI CCNNNNNNNGG 1 cut(s) 202
BsmAI GTCTC 1 cut(s) 347
BsmFI GGGAC 1 cut(s) 320
BsnI GGCC 1 cut(s) 267
Bsp143I GATC 5 cut(s) 91, 192, 208, 229, 241
Bsp19I CCATGG 1 cut(s) 269
BspACI CCGC 1 cut(s) 329
BspANI GGCC 1 cut(s) 267
BspLI GGNNCC 2 cut(s) 64, 124
BspPI GGATC 3 cut(s) 99, 187, 216
BsrI ACTGG 1 cut(s) 323
BssECI CCNNGG 3 cut(s) 66, 140, 269
BssMI GATC 5 cut(s) 91, 192, 208, 229, 241
BssT1I CCWWGG 1 cut(s) 269
Bst2UI CCWGG 1 cut(s) 141
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 1 cut(s) 357
BstDSI CCRYGG 2 cut(s) 66, 269
BstHHI GCGC 1 cut(s) 37
BstKTI GATC 5 cut(s) 94, 195, 211, 232, 244
BstMAI GTCTC 1 cut(s) 347
BstMBI GATC 5 cut(s) 91, 192, 208, 229, 241
BstMCI CGRYCG 1 cut(s) 232
BstNI CCWGG 1 cut(s) 141
BstSCI CCNGG 1 cut(s) 139
BsuRI GGCC 1 cut(s) 267
BtgI CCRYGG 2 cut(s) 66, 269
BtsIMutI CAGTG 1 cut(s) 330
Cac8I GCNNGC 1 cut(s) 7
CfoI GCGC 1 cut(s) 37
Cfr13I GGNCC 3 cut(s) 122, 266, 320
Csp6I GTAC 2 cut(s) 100, 307
CviAII CATG 5 cut(s) 38, 103, 260, 270, 289
CviJI RGCY 8 cut(s) 5, 21, 42, 73, 80, 131, 187, 267
CviKI_1 RGCY 8 cut(s) 5, 21, 42, 73, 80, 131, 187, 267
CviQI GTAC 2 cut(s) 100, 307
DdeI CTNAG 1 cut(s) 357
DpnI GATC 5 cut(s) 93, 194, 210, 231, 243
DpnII GATC 5 cut(s) 91, 192, 208, 229, 241
EciI GGCGGA 1 cut(s) 344
Eco130I CCWWGG 1 cut(s) 269
Eco47I GGWCC 2 cut(s) 122, 320
Eco57I CTGAAG 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 269
ErhI CCWWGG 1 cut(s) 269
FaeI CATG 5 cut(s) 41, 106, 263, 273, 292
FaiI YATR 6 cut(s) 39, 104, 261, 271, 279, 290
FaqI GGGAC 1 cut(s) 320
FatI CATG 5 cut(s) 37, 102, 259, 269, 288
GlaI GCGC 1 cut(s) 36
GsaI CCCAGC 2 cut(s) 62, 302
GsuI CTGGAG 1 cut(s) 190
HaeIII GGCC 1 cut(s) 267
HhaI GCGC 1 cut(s) 37
Hin1II CATG 5 cut(s) 41, 106, 263, 273, 292
Hin6I GCGC 1 cut(s) 35
HinP1I GCGC 1 cut(s) 35
HinfI GANTC 1 cut(s) 175
Hpy166II GTNNAC 2 cut(s) 116, 307
Hpy188I TCNGA 2 cut(s) 84, 246
Hpy188III TCNNGA 2 cut(s) 169, 233
Hpy8I GTNNAC 2 cut(s) 116, 307
HpyAV CCTTC 1 cut(s) 79
HpyCH4V TGCA 2 cut(s) 47, 259
HpyF3I CTNAG 1 cut(s) 357
Hsp92II CATG 5 cut(s) 41, 106, 263, 273, 292
HspAI GCGC 1 cut(s) 35
Kzo9I GATC 5 cut(s) 91, 192, 208, 229, 241
LpnPI CCDG 6 cut(s) 44, 126, 153, 154, 284, 336
MalI GATC 5 cut(s) 93, 194, 210, 231, 243
MboI GATC 5 cut(s) 91, 192, 208, 229, 241
MnlI CCTC 4 cut(s) 70, 165, 257, 348
MseI TTAA 1 cut(s) 164
MslI CAYNNNNRTG 1 cut(s) 293
MspR9I CCNGG 1 cut(s) 141
MvaI CCWGG 1 cut(s) 141
NcoI CCATGG 1 cut(s) 269
NdeII GATC 5 cut(s) 91, 192, 208, 229, 241
NlaIII CATG 5 cut(s) 41, 106, 263, 273, 292
NlaIV GGNNCC 2 cut(s) 64, 124
PcsI WCGNNNNNNNCGW 1 cut(s) 188
PfeI GAWTC 1 cut(s) 175
PflMI CCANNNNNTGG 1 cut(s) 202
Ple19I CGATCG 1 cut(s) 232
Psp6I CCWGG 1 cut(s) 139
PspFI CCCAGC 2 cut(s) 58, 298
PspGI CCWGG 1 cut(s) 139
PspN4I GGNNCC 2 cut(s) 64, 124
PspPI GGNCC 3 cut(s) 122, 266, 320
PvuI CGATCG 1 cut(s) 232
RsaI GTAC 2 cut(s) 101, 308
RsaNI GTAC 2 cut(s) 100, 307
RseI CAYNNNNRTG 1 cut(s) 293
SaqAI TTAA 1 cut(s) 164
Sau3AI GATC 5 cut(s) 91, 192, 208, 229, 241
Sau96I GGNCC 3 cut(s) 122, 266, 320
ScrFI CCNGG 1 cut(s) 141
SetI ASST 1 cut(s) 90
SinI GGWCC 2 cut(s) 122, 320
SmiMI CAYNNNNRTG 1 cut(s) 293
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
SsiI CCGC 1 cut(s) 329
SspI AATATT 1 cut(s) 255
StyD4I CCNGG 1 cut(s) 139
StyI CCWWGG 1 cut(s) 269
TaqI TCGA 2 cut(s) 228, 232
TfiI GAWTC 1 cut(s) 175
Tru1I TTAA 1 cut(s) 164
Tru9I TTAA 1 cut(s) 164
TscAI CASTG 1 cut(s) 330
TspDTI ATGAA 1 cut(s) 212
TspGWI ACGGA 1 cut(s) 55
TspRI CASTG 1 cut(s) 330
Van91I CCANNNNNTGG 1 cut(s) 202
VpaK11BI GGWCC 2 cut(s) 122, 320
XcmI CCANNNNNNNNNTGG 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.