pycom15g19530

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0013113
Physical Location & Seq
Forward (+)
13929858 .. 13935055
5198 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1476 bp
ATGGTGGAGACTTTGCCACTAGGGCGGTTCCACCACCAGAGGCTGGACCTGAAGCGCTGGTGCCCCGCCTTCTTATCCTCCCACAAAACCCTCTTCACCGTGCTCTGGATCGCCGCTTTCGCCTCAGTTTTCGTGTGGCAAAGGAACATTGTAAATGGGTTTTCGATATTCCGGCGAGCTTCGGTGAGGGAGATGCCGAAGCTGCGGCCGGTGGCTTTCAATTTGACGGATTTCGGGGCAGTGGGCGATGGGGTGACTTTGAACACTGAGGCGTTTGAGAAGGCGGTGCTGGCTATTTCTAAGCTGGGGAAGAAAGGCGGTGGGCAGCTCAATGTGCCTCCAGGCCGGTGGCTTACGGCGCCGTTTAATCTCACTAGTCACATGACTCTGTTTCTTGCTGAAGATGCTGAGATACTTGGGATTCAGGATGAGAAGTATTGGCCCTTGATGCCTCCATTGCCTTCCTATGGGTATGGGAGAGAGCATCCTGGGCCCCGATATGGAAGTCTGATTCATGGTCAAAATCTTAAAGATATTATTATAACAGGGCATAATGGTACCATAAATGGACAGGGTCAAACATGGTGGAAGAAGTACCTCCAGAAGCTTCTCAACAACACTCGGGGTCCACTTGTTCAGATTATGTGGTCTAGCGACATTTTGATCAGTAATATAACCTTGCGTGATTCTCCTTTTTGGACACTCCATCCATATGACTGCAAGAACATTACAATTAGAAATGTTACAATTTTGGCTCCCATACGTAAAGCTCCAAATACTGATGGAATAGATCCTGACTCATGTGAGGATATGGTGATTGAGGACTGCTACATAAGTGTAGGGGATGATGGAATTGCAATAAAGAGTGGCTGGGATCAATATGGTATTTCTTATGGACGGCCATCAAAGAATATACTCATTAGAAATCTTGTTGTCCGCTCTATGGTCAGTGCTGGCGTATCAATCGGCAGTGAGATGTCTGGTGGGGTTTCAAATGTCACGGTGGAGAACTTGCTTGTTTGGAGCTCAAGGCGTGCTGTTCGGATCAAGACTGCCCCTGGAAGAGGTGGATATGTGCGACAAATAACCTATCGGAATCTGACATTTGACAATGTCCGAGTTGGAATTGTTATCAAGACAGACTACAATGAGCACCCCGACGGGGGCTATGATCCAAGGGCTCTTCCAGTGCTCGAGGACATAAGCTTCACAAGCGTCATTGGACAGGGAGTTCGGGTGCCTGTCCGTATCCATGGAAGTGAAGAGATTCCAGTCAGAAATGTGACTTTCCGGGATATGTCGGTGGGGCTAACATACAAAAAGAAGCACATTTTCCAGTGTGCCTTTGTTCAAGGTCGTGTAATAGGTACCATCTTCCCTGCCCCTTGCGAAAATCTTGACCGGTATGATGAGCAAGGAAAGCTGGTGAAACACTCTGCGTCCCAGAACATGACAGAAATAGATTATGATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

492

Amino Acids

55.01

Weight (kDa)

9.12

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 169 - 450 2.9e-47 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013113)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19170 AT3G42950
fragaria_vesca FvH4_1g09050 FvH4_1g09050
malus_domestica MD02G1096000.v1.1
prunus_persica Prupe.7G195000_v2.0.a1
pyrus_communis pycom02g07580 pycom15g19530
rosa_chinensis RchiOBHm_Chr2g0095471
rosa_laevigata RLG00000016570
rosa_multiflora Rmu_sc0006076.1_g000011
rosa_roxburghii Rroxscaffold_2G00146310
rosa_rugosa Rorug02G0051400.1
rosa_samantha Rh2AG099400 Rh2BG100700 Rh2CG102800 Rh2DG100300
rosa_wichuraiana Rw2G007530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 542
Acc65I GGTACC 2 cut(s) 557, 1367
AccB1I GGYRCC 5 cut(s) 60, 358, 557, 1237, 1367
AccB7I CCANNNNNTGG 1 cut(s) 43
AccBSI CCGCTC 1 cut(s) 939
AciI CCGC 7 cut(s) 25, 66, 114, 205, 284, 318, 937
AclWI GGATC 5 cut(s) 116, 785, 882, 1052, 1166
AcoI YGGCCR 2 cut(s) 206, 899
AcuI CTGAAG 2 cut(s) 71, 420
AcyI GRCGYC 1 cut(s) 359
AfaI GTAC 3 cut(s) 559, 596, 1369
AfeI AGCGCT 1 cut(s) 56
AfiI CCNNNNNNNGG 8 cut(s) 43, 105, 467, 500, 943, 1064, 1162, 1163
AgeI ACCGGT 1 cut(s) 1401
AgsI TTSAA 4 cut(s) 220, 262, 993, 1352
AhlI ACTAGT 1 cut(s) 374
AjnI CCWGG 3 cut(s) 340, 487, 1057
AloI GAACNNNNNNTCC 2 cut(s) 1215, 1247
AluBI AGCT 9 cut(s) 179, 202, 304, 328, 607, 770, 1026, 1206, 1423
AluI AGCT 9 cut(s) 179, 202, 304, 328, 607, 770, 1026, 1206, 1423
Alw21I GWGCWC 4 cut(s) 105, 1028, 1155, 1194
Alw26I GTCTC 1 cut(s) 2
AlwI GGATC 5 cut(s) 116, 785, 882, 1052, 1166
AlwNI CAGNNNCTG 1 cut(s) 43
Ama87I CYCGRG 2 cut(s) 621, 1193
Aor51HI AGCGCT 1 cut(s) 56
AoxI GGCC 5 cut(s) 206, 343, 440, 491, 899
ApaI GGGCCC 1 cut(s) 495
ApeKI GCWGC 2 cut(s) 202, 325
ArsI GACNNNNNNTTYG 2 cut(s) 1215, 1247
AsiGI ACCGGT 1 cut(s) 1401
Asp700I GAANNNNTTC 1 cut(s) 1266
Asp718I GGTACC 2 cut(s) 557, 1367
AspLEI GCGC 2 cut(s) 57, 361
AspS9I GGNCC 5 cut(s) 46, 441, 491, 492, 626
AsuC2I CCSGG 1 cut(s) 1292
AsuHPI GGTGA 5 cut(s) 88, 196, 265, 826, 1438
AvaI CYCGRG 2 cut(s) 621, 1193
AvaII GGWCC 2 cut(s) 46, 626
BaeGI GKGCMC 2 cut(s) 65, 495
BanI GGYRCC 5 cut(s) 60, 358, 557, 1237, 1367
BanII GRGCYC 3 cut(s) 495, 1028, 1183
Bbv12I GWGCWC 4 cut(s) 105, 1028, 1155, 1194
BbvI GCAGC 2 cut(s) 189, 337
BccI CCATC 6 cut(s) 242, 714, 776, 842, 910, 1379
BceAI ACGGC 3 cut(s) 346, 372, 914
BciT130I CCWGG 3 cut(s) 342, 489, 1059
BciVI GTATCC 1 cut(s) 1259
BclI TGATCA 1 cut(s) 663
BcnI CCSGG 1 cut(s) 1292
BcoDI GTCTC 1 cut(s) 2
BcuI ACTAGT 1 cut(s) 374
BfaI CTAG 3 cut(s) 20, 375, 651
BfoI RGCGCY 2 cut(s) 58, 362
BfuI GTATCC 1 cut(s) 1259
BglI GCCNNNNNGGC 1 cut(s) 22
BisI GCNGC 4 cut(s) 114, 203, 206, 326
BlsI GCNGC 4 cut(s) 115, 204, 207, 327
Bme1390I CCNGG 4 cut(s) 342, 489, 1059, 1292
Bme18I GGWCC 2 cut(s) 46, 626
BmeT110I CYCGRG 2 cut(s) 621, 1193
BmgT120I GGNCC 5 cut(s) 46, 441, 491, 492, 626
BmrFI CCNGG 4 cut(s) 342, 489, 1059, 1292
BmsI GCATC 4 cut(s) 183, 394, 438, 493
BpmI CTGGAG 2 cut(s) 324, 584
BpuEI CTTGAG 1 cut(s) 1012
BpuMI CCSGG 1 cut(s) 1292
BsaAI YACGTR 1 cut(s) 764
BsaHI GRCGYC 1 cut(s) 359
BsaJI CCNNGG 4 cut(s) 488, 1057, 1175, 1252
BsaWI WCCGGW 1 cut(s) 1401
BsaXI ACNNNNNCTCC 2 cut(s) 1221, 1251
Bsc4I CCNNNNNNNGG 8 cut(s) 43, 105, 467, 500, 943, 1064, 1162, 1163
Bse118I RCCGGY 3 cut(s) 208, 345, 1401
Bse1I ACTGG 3 cut(s) 1187, 1271, 1336
Bse3DI GCAATG 1 cut(s) 455
BseBI CCWGG 3 cut(s) 342, 489, 1059
BseDI CCNNGG 4 cut(s) 488, 1057, 1175, 1252
BseGI GGATG 4 cut(s) 433, 484, 706, 850
BseLI CCNNNNNNNGG 8 cut(s) 43, 105, 467, 500, 943, 1064, 1162, 1163
BseMI GCAATG 1 cut(s) 455
BseMII CTCAG 3 cut(s) 138, 258, 399
BseNI ACTGG 3 cut(s) 1187, 1271, 1336
BseSI GKGCMC 2 cut(s) 65, 495
BseX3I CGGCCG 1 cut(s) 206
BseXI GCAGC 2 cut(s) 189, 337
BseYI CCCAGC 2 cut(s) 304, 870
Bsh1285I CGRYCG 1 cut(s) 209
BshFI GGCC 5 cut(s) 208, 345, 442, 493, 901
BshNI GGYRCC 5 cut(s) 60, 358, 557, 1237, 1367
BshTI ACCGGT 1 cut(s) 1401
BsiEI CGRYCG 1 cut(s) 209
BsiHKAI GWGCWC 4 cut(s) 105, 1028, 1155, 1194
BsiHKCI CYCGRG 2 cut(s) 621, 1193
BsiSI CCGG 5 cut(s) 172, 209, 346, 1291, 1402
BslFI GGGAC 1 cut(s) 1426
BslI CCNNNNNNNGG 8 cut(s) 43, 105, 467, 500, 943, 1064, 1162, 1163
BsmAI GTCTC 1 cut(s) 2
BsmFI GGGAC 1 cut(s) 1426
BsnI GGCC 5 cut(s) 208, 345, 442, 493, 901
BsoBI CYCGRG 2 cut(s) 621, 1193
Bsp120I GGGCCC 1 cut(s) 491
Bsp1286I GDGCHC 7 cut(s) 65, 105, 495, 1028, 1155, 1183, 1194
Bsp143I GATC 6 cut(s) 108, 663, 790, 874, 1044, 1171
Bsp19I CCATGG 1 cut(s) 1252
BspACI CCGC 7 cut(s) 25, 66, 114, 205, 284, 318, 937
BspANI GGCC 5 cut(s) 208, 345, 442, 493, 901
BspCNI CTCAG 3 cut(s) 137, 259, 400
BspPI GGATC 5 cut(s) 116, 785, 882, 1052, 1166
BspQI GCTCTTC 1 cut(s) 1188
BspT107I GGYRCC 5 cut(s) 60, 358, 557, 1237, 1367
BsrBI CCGCTC 1 cut(s) 939
BsrDI GCAATG 1 cut(s) 455
BsrFI RCCGGY 3 cut(s) 208, 345, 1401
BsrI ACTGG 3 cut(s) 1187, 1271, 1336
BssAI RCCGGY 3 cut(s) 208, 345, 1401
BssECI CCNNGG 4 cut(s) 488, 1057, 1175, 1252
BssMI GATC 6 cut(s) 108, 663, 790, 874, 1044, 1171
BssNI GRCGYC 1 cut(s) 359
BssT1I CCWWGG 2 cut(s) 1175, 1252
Bst2UI CCWGG 3 cut(s) 342, 489, 1059
Bst4CI ACNGT 2 cut(s) 100, 1003
Bst6I CTCTTC 4 cut(s) 98, 1057, 1188, 1257
BstACI GRCGYC 1 cut(s) 359
BstBAI YACGTR 1 cut(s) 764
BstC8I GCNNGC 4 cut(s) 177, 291, 955, 1035
BstDEI CTNAG 4 cut(s) 124, 267, 300, 408
BstDSI CCRYGG 1 cut(s) 1252
BstENI CCTNNNNNAGG 1 cut(s) 1062
BstF5I GGATG 4 cut(s) 433, 484, 706, 850
BstH2I RGCGCY 2 cut(s) 58, 362
BstHHI GCGC 2 cut(s) 57, 361
BstKTI GATC 6 cut(s) 111, 666, 793, 877, 1047, 1174
BstMAI GTCTC 1 cut(s) 2
BstMBI GATC 6 cut(s) 108, 663, 790, 874, 1044, 1171
BstMCI CGRYCG 1 cut(s) 209
BstNI CCWGG 3 cut(s) 342, 489, 1059
BstSCI CCNGG 4 cut(s) 340, 487, 1057, 1290
BstSLI GKGCMC 2 cut(s) 65, 495
BstSNI TACGTA 1 cut(s) 764
BstV1I GCAGC 2 cut(s) 189, 337
BstX2I RGATCY 1 cut(s) 790
BstXI CCANNNNNNTGG 1 cut(s) 348
BstYI RGATCY 1 cut(s) 790
BstZI CGGCCG 1 cut(s) 206
BsuI GTATCC 1 cut(s) 1259
BsuRI GGCC 5 cut(s) 208, 345, 442, 493, 901
BtgI CCRYGG 1 cut(s) 1252
BtgZI GCGATG 1 cut(s) 261
BtsCI GGATG 4 cut(s) 433, 484, 706, 850
BtsI GCAGTG 2 cut(s) 246, 976
BtsIMutI CAGTG 6 cut(s) 246, 264, 955, 976, 1194, 1343
Cac8I GCNNGC 4 cut(s) 177, 291, 955, 1035
CaiI CAGNNNCTG 1 cut(s) 43
CfoI GCGC 2 cut(s) 57, 361
Cfr10I RCCGGY 3 cut(s) 208, 345, 1401
Cfr13I GGNCC 5 cut(s) 46, 441, 491, 492, 626
CseI GACGC 2 cut(s) 1204, 1428
Csp6I GTAC 3 cut(s) 558, 595, 1368
CspAI ACCGGT 1 cut(s) 1401
CspCI CAANNNNNGTGG 2 cut(s) 566, 601
CviAII CATG 6 cut(s) 382, 515, 582, 801, 1253, 1450
CviQI GTAC 3 cut(s) 558, 595, 1368
DdeI CTNAG 4 cut(s) 124, 267, 300, 408
DinI GGCGCC 1 cut(s) 360
DpnI GATC 6 cut(s) 110, 665, 792, 876, 1046, 1173
DpnII GATC 6 cut(s) 108, 663, 790, 874, 1044, 1171
EaeI YGGCCR 2 cut(s) 206, 899
EagI CGGCCG 1 cut(s) 206
Eam1104I CTCTTC 4 cut(s) 98, 1057, 1188, 1257
EarI CTCTTC 4 cut(s) 98, 1057, 1188, 1257
Ecl136II GAGCTC 1 cut(s) 1026
EclXI CGGCCG 1 cut(s) 206
Eco105I TACGTA 1 cut(s) 764
Eco130I CCWWGG 2 cut(s) 1175, 1252
Eco24I GRGCYC 3 cut(s) 495, 1028, 1183
Eco47I GGWCC 2 cut(s) 46, 626
Eco47III AGCGCT 1 cut(s) 56
Eco52I CGGCCG 1 cut(s) 206
Eco53kI GAGCTC 1 cut(s) 1026
Eco57I CTGAAG 2 cut(s) 71, 420
Eco88I CYCGRG 2 cut(s) 621, 1193
EcoICRI GAGCTC 1 cut(s) 1026
EcoNI CCTNNNNNAGG 1 cut(s) 1062
EcoO109I RGGNCCY 1 cut(s) 492
EcoRII CCWGG 3 cut(s) 340, 487, 1057
EcoT14I CCWWGG 2 cut(s) 1175, 1252
EcoT38I GRGCYC 3 cut(s) 495, 1028, 1183
EgeI GGCGCC 1 cut(s) 360
EheI GGCGCC 1 cut(s) 360
ErhI CCWWGG 2 cut(s) 1175, 1252
FaeI CATG 6 cut(s) 385, 518, 585, 804, 1256, 1453
FaqI GGGAC 1 cut(s) 1426
FatI CATG 6 cut(s) 381, 514, 581, 800, 1252, 1449
FauI CCCGC 1 cut(s) 73
FauNDI CATATG 1 cut(s) 712
FbaI TGATCA 1 cut(s) 663
Fnu4HI GCNGC 4 cut(s) 114, 203, 206, 326
FokI GGATG 4 cut(s) 440, 471, 693, 857
FriOI GRGCYC 3 cut(s) 495, 1028, 1183
Fsp4HI GCNGC 4 cut(s) 114, 203, 206, 326
FspBI CTAG 3 cut(s) 20, 375, 651
GlaI GCGC 2 cut(s) 56, 360
GluI GCNGC 4 cut(s) 114, 203, 206, 326
GsaI CCCAGC 2 cut(s) 308, 874
GsuI CTGGAG 2 cut(s) 324, 584
HaeII RGCGCY 2 cut(s) 58, 362
HaeIII GGCC 5 cut(s) 208, 345, 442, 493, 901
HapII CCGG 5 cut(s) 172, 209, 346, 1291, 1402
HgaI GACGC 2 cut(s) 1204, 1428
HhaI GCGC 2 cut(s) 57, 361
Hin1I GRCGYC 1 cut(s) 359
Hin1II CATG 6 cut(s) 385, 518, 585, 804, 1256, 1453
Hin6I GCGC 2 cut(s) 55, 359
HinP1I GCGC 2 cut(s) 55, 359
HindIII AAGCTT 2 cut(s) 605, 1204
HinfI GANTC 7 cut(s) 385, 421, 511, 686, 797, 1096, 1267
HpaII CCGG 5 cut(s) 172, 209, 346, 1291, 1402
HphI GGTGA 5 cut(s) 88, 196, 265, 826, 1438
Hpy166II GTNNAC 1 cut(s) 629
Hpy188I TCNGA 7 cut(s) 510, 639, 1044, 1095, 1101, 1118, 1277
Hpy188III TCNNGA 7 cut(s) 106, 425, 601, 794, 1048, 1135, 1397
Hpy8I GTNNAC 1 cut(s) 629
Hpy99I CGWCG 1 cut(s) 1163
HpyAV CCTTC 3 cut(s) 79, 274, 471
HpyCH4III ACNGT 2 cut(s) 100, 1003
HpyCH4IV ACGT 1 cut(s) 763
HpyCH4V TGCA 2 cut(s) 720, 857
HpyF3I CTNAG 4 cut(s) 124, 267, 300, 408
HpySE526I ACGT 1 cut(s) 763
Hsp92I GRCGYC 1 cut(s) 359
Hsp92II CATG 6 cut(s) 385, 518, 585, 804, 1256, 1453
HspAI GCGC 2 cut(s) 55, 359
KasI GGCGCC 1 cut(s) 358
KpnI GGTACC 2 cut(s) 561, 1371
Ksp22I TGATCA 1 cut(s) 663
Kzo9I GATC 6 cut(s) 108, 663, 790, 874, 1044, 1171
LguI GCTCTTC 1 cut(s) 1188
LmnI GCTCC 3 cut(s) 760, 775, 1023
Lsp1109I GCAGC 2 cut(s) 189, 337
LweI GCATC 4 cut(s) 183, 394, 438, 493
MaeI CTAG 3 cut(s) 20, 375, 651
MaeII ACGT 1 cut(s) 763
MaeIII GTNAC 5 cut(s) 253, 377, 742, 997, 1282
MalI GATC 6 cut(s) 110, 665, 792, 876, 1046, 1173
MbiI CCGCTC 1 cut(s) 939
MboI GATC 6 cut(s) 108, 663, 790, 874, 1044, 1171
MboII GAAGA 8 cut(s) 85, 322, 413, 601, 1074, 1175, 1274, 1366
MflI RGATCY 1 cut(s) 790
MhlI GDGCHC 7 cut(s) 65, 105, 495, 1028, 1155, 1183, 1194
MluCI AATT 5 cut(s) 220, 732, 747, 852, 1125
Mly113I GGCGCC 1 cut(s) 359
MlyI GAGTC 2 cut(s) 379, 791
MmeI TCCRAC 1 cut(s) 1102
MroXI GAANNNNTTC 1 cut(s) 1266
MseI TTAA 2 cut(s) 366, 528
MslI CAYNNNNRTG 2 cut(s) 711, 1257
MspI CCGG 5 cut(s) 172, 209, 346, 1291, 1402
MspR9I CCNGG 4 cut(s) 342, 489, 1059, 1292
MvaI CCWGG 3 cut(s) 342, 489, 1059
NarI GGCGCC 1 cut(s) 359
NciI CCSGG 1 cut(s) 1292
NcoI CCATGG 1 cut(s) 1252
NdeI CATATG 1 cut(s) 712
NdeII GATC 6 cut(s) 108, 663, 790, 874, 1044, 1171
NlaIII CATG 6 cut(s) 385, 518, 585, 804, 1256, 1453
NmuCI GTSAC 4 cut(s) 253, 377, 997, 1282
PaeR7I CTCGAG 1 cut(s) 1193
PciSI GCTCTTC 1 cut(s) 1188
PdmI GAANNNNTTC 1 cut(s) 1266
PfeI GAWTC 5 cut(s) 421, 511, 686, 1096, 1267
PflFI GACNNNGTC 2 cut(s) 573, 1112
PflMI CCANNNNNTGG 1 cut(s) 43
PfoI TCCNGGA 1 cut(s) 1290
PinAI ACCGGT 1 cut(s) 1401
PkrI GCNGC 4 cut(s) 115, 204, 207, 327
PleI GAGTC 2 cut(s) 379, 791
PluTI GGCGCC 1 cut(s) 362
PpsI GAGTC 2 cut(s) 379, 791
Ppu21I YACGTR 1 cut(s) 764
PsiI TTATAA 1 cut(s) 542
Psp124BI GAGCTC 1 cut(s) 1028
Psp6I CCWGG 3 cut(s) 340, 487, 1057
PspFI CCCAGC 2 cut(s) 304, 870
PspGI CCWGG 3 cut(s) 340, 487, 1057
PspOMI GGGCCC 1 cut(s) 491
PspPI GGNCC 5 cut(s) 46, 441, 491, 492, 626
PspXI VCTCGAGB 1 cut(s) 1193
PstNI CAGNNNCTG 1 cut(s) 43
PsuI RGATCY 1 cut(s) 790
PsyI GACNNNGTC 2 cut(s) 573, 1112
RsaI GTAC 3 cut(s) 559, 596, 1369
RsaNI GTAC 3 cut(s) 558, 595, 1368
RseI CAYNNNNRTG 2 cut(s) 711, 1257
SacI GAGCTC 1 cut(s) 1028
SapI GCTCTTC 1 cut(s) 1188
SaqAI TTAA 2 cut(s) 366, 528
SatI GCNGC 4 cut(s) 114, 203, 206, 326
Sau3AI GATC 6 cut(s) 108, 663, 790, 874, 1044, 1171
Sau96I GGNCC 5 cut(s) 46, 441, 491, 492, 626
SchI GAGTC 2 cut(s) 379, 791
ScrFI CCNGG 4 cut(s) 342, 489, 1059, 1292
SduI GDGCHC 7 cut(s) 65, 105, 495, 1028, 1155, 1183, 1194
SfaNI GCATC 4 cut(s) 183, 394, 438, 493
SfoI GGCGCC 1 cut(s) 360
Sfr274I CTCGAG 1 cut(s) 1193
SinI GGWCC 2 cut(s) 46, 626
SlaI CTCGAG 1 cut(s) 1193
SmiMI CAYNNNNRTG 2 cut(s) 711, 1257
SmlI CTYRAG 2 cut(s) 1027, 1193
SmoI CTYRAG 2 cut(s) 1027, 1193
SnaBI TACGTA 1 cut(s) 764
SpeI ACTAGT 1 cut(s) 374
Sse9I AATT 5 cut(s) 220, 732, 747, 852, 1125
SsiI CCGC 7 cut(s) 25, 66, 114, 205, 284, 318, 937
SspDI GGCGCC 1 cut(s) 358
SspMI CTAG 3 cut(s) 20, 375, 651
SstI GAGCTC 1 cut(s) 1028
StyD4I CCNGG 4 cut(s) 340, 487, 1057, 1290
StyI CCWWGG 2 cut(s) 1175, 1252
TaaI ACNGT 2 cut(s) 100, 1003
TaiI ACGT 1 cut(s) 766
TaqI TCGA 2 cut(s) 164, 1194
TasI AATT 5 cut(s) 220, 732, 747, 852, 1125
TauI GCSGC 2 cut(s) 116, 208
TfiI GAWTC 5 cut(s) 421, 511, 686, 1096, 1267
Tru1I TTAA 2 cut(s) 366, 528
Tru9I TTAA 2 cut(s) 366, 528
TscAI CASTG 6 cut(s) 246, 271, 955, 976, 1194, 1343
TseFI GTSAC 4 cut(s) 253, 377, 997, 1282
TseI GCWGC 2 cut(s) 202, 325
Tsp45I GTSAC 4 cut(s) 253, 377, 997, 1282
TspDTI ATGAA 1 cut(s) 503
TspGWI ACGGA 2 cut(s) 242, 1235
TspRI CASTG 6 cut(s) 246, 271, 955, 976, 1194, 1343
Tth111I GACNNNGTC 2 cut(s) 573, 1112
Van91I CCANNNNNTGG 1 cut(s) 43
VpaK11BI GGWCC 2 cut(s) 46, 626
XagI CCTNNNNNAGG 1 cut(s) 1062
XhoI CTCGAG 1 cut(s) 1193
XmnI GAANNNNTTC 1 cut(s) 1266
XspI CTAG 3 cut(s) 20, 375, 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.