pycom15g22810

Nucleolar protein 6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0019830
Physical Location & Seq
Forward (+)
16913431 .. 16914403
973 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 516 bp
ATGGGCTTAGGAGAGAAGAATAATTCGACCAAGAATTTAGCCCAGAAAATAATTTGGGCCGGCTATGAAGCTTTATCGGCCACCAAAACAACCCTGTTTCAGCTCACAAAGCAGAGCCAAAGCCGACGAAGAACAATGGAGCCCGCTACGCTGACGAACTCAATGGACCTGAAGATTGGGGAGCTCTTGAAGGAGGTCCAGCTCGACTATTCCCCTGCATTCACCAAGGCCGTGGACGACACCGTTTCAGCCATCAAGGACGCCATGGACAAAATCCCAGAAGGGTTCAATGTGACAGCTAACGAAGCTCCAGGATTCATCAGAGACATAGAAGCTGACAAGGTCGAATTCACGTTTAAGAAGCCGAATTCTTTTGAAATTGGTGGGAGCTATGCCATACAGTGCATTGTAAAACCTGAGGTCAATGTGGACCTTTTCGTTCAAATGCCCAAGGAGTGTTTCCATGAGAAGGACTATTTGAATTACCGGCGAAAAGGTGCCTGTACCTTTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
Nrap Nrap_D2 Nrap_D3 Nrap_D4 Nrap_D5 Nrap_D6

Protein Analysis

172

Amino Acids

19.23

Weight (kDa)

6.61

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nrap PF03813 143 - 163 5.1e-07 Nrap protein domain 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019830)

Species Orthologous Gene IDs
pyrus_communis pycom15g22810
rosa_roxburghii Rroxscaffold_6G00403890 Rroxscaffold_6G00404580
rosa_rugosa Rorug03G0161600
rosa_samantha Rh3AG211600 Rh3CG239000 Rh3DG238500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 497
AciI CCGC 1 cut(s) 144
AcoI YGGCCR 1 cut(s) 78
AcsI RAATTY 3 cut(s) 34, 347, 367
AcuI CTGAAG 1 cut(s) 191
AcyI GRCGYC 1 cut(s) 261
AfaI GTAC 1 cut(s) 505
AfiI CCNNNNNNNGG 1 cut(s) 469
AgsI TTSAA 5 cut(s) 190, 289, 377, 443, 481
AjnI CCWGG 1 cut(s) 310
AjuI GAANNNNNNNTTGG 2 cut(s) 443, 475
AluBI AGCT 8 cut(s) 71, 103, 184, 202, 299, 308, 335, 390
AluI AGCT 8 cut(s) 71, 103, 184, 202, 299, 308, 335, 390
Alw21I GWGCWC 1 cut(s) 186
Alw26I GTCTC 1 cut(s) 318
AoxI GGCC 3 cut(s) 57, 78, 228
ApoI RAATTY 3 cut(s) 34, 347, 367
AspS9I GGNCC 4 cut(s) 57, 166, 196, 430
AsuHPI GGTGA 1 cut(s) 214
AvaII GGWCC 3 cut(s) 166, 196, 430
AxyI CCTNAGG 1 cut(s) 417
BanI GGYRCC 1 cut(s) 497
BanII GRGCYC 2 cut(s) 144, 186
Bbv12I GWGCWC 1 cut(s) 186
BccI CCATC 1 cut(s) 260
BceAI ACGGC 1 cut(s) 215
BciT130I CCWGG 1 cut(s) 312
BcoDI GTCTC 1 cut(s) 318
Bme1390I CCNGG 1 cut(s) 312
Bme18I GGWCC 3 cut(s) 166, 196, 430
BmgT120I GGNCC 4 cut(s) 57, 166, 196, 430
BmiI GGNNCC 2 cut(s) 141, 499
BmrFI CCNGG 1 cut(s) 312
BpmI CTGGAG 1 cut(s) 294
Bpu10I CCTNAGC 1 cut(s) 7
BsaHI GRCGYC 1 cut(s) 261
BsaJI CCNNGG 4 cut(s) 225, 231, 264, 450
Bsc4I CCNNNNNNNGG 1 cut(s) 469
Bse118I RCCGGY 2 cut(s) 59, 486
Bse21I CCTNAGG 1 cut(s) 417
BseBI CCWGG 1 cut(s) 312
BseDI CCNNGG 4 cut(s) 225, 231, 264, 450
BseLI CCNNNNNNNGG 1 cut(s) 469
BseMII CTCAG 1 cut(s) 408
BshFI GGCC 3 cut(s) 59, 80, 230
BshNI GGYRCC 1 cut(s) 497
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 2 cut(s) 60, 487
BslI CCNNNNNNNGG 1 cut(s) 469
BsmAI GTCTC 1 cut(s) 318
BsmI GAATGC 1 cut(s) 218
BsnI GGCC 3 cut(s) 59, 80, 230
Bsp1286I GDGCHC 2 cut(s) 144, 186
Bsp19I CCATGG 1 cut(s) 264
BspACI CCGC 1 cut(s) 144
BspANI GGCC 3 cut(s) 59, 80, 230
BspCNI CTCAG 1 cut(s) 409
BspLI GGNNCC 2 cut(s) 141, 499
BspT107I GGYRCC 1 cut(s) 497
BsrFI RCCGGY 2 cut(s) 59, 486
BssAI RCCGGY 2 cut(s) 59, 486
BssECI CCNNGG 4 cut(s) 225, 231, 264, 450
BssNI GRCGYC 1 cut(s) 261
BssT1I CCWWGG 3 cut(s) 225, 264, 450
Bst2UI CCWGG 1 cut(s) 312
Bst4CI ACNGT 2 cut(s) 244, 402
BstACI GRCGYC 1 cut(s) 261
BstC8I GCNNGC 2 cut(s) 61, 144
BstDEI CTNAG 2 cut(s) 7, 417
BstDSI CCRYGG 2 cut(s) 231, 264
BstMAI GTCTC 1 cut(s) 318
BstMWI GCNNNNNNNGC 4 cut(s) 77, 109, 148, 305
BstNI CCWGG 1 cut(s) 312
BstSCI CCNGG 1 cut(s) 310
BstXI CCANNNNNNTGG 1 cut(s) 232
Bsu36I CCTNAGG 1 cut(s) 417
BsuRI GGCC 3 cut(s) 59, 80, 230
BtgI CCRYGG 2 cut(s) 231, 264
BtsIMutI CAGTG 1 cut(s) 407
Cac8I GCNNGC 2 cut(s) 61, 144
Cfr10I RCCGGY 2 cut(s) 59, 486
Cfr13I GGNCC 4 cut(s) 57, 166, 196, 430
CseI GACGC 1 cut(s) 269
Csp6I GTAC 1 cut(s) 504
CviAII CATG 2 cut(s) 265, 464
CviQI GTAC 1 cut(s) 504
DdeI CTNAG 2 cut(s) 7, 417
EaeI YGGCCR 1 cut(s) 78
Ecl136II GAGCTC 1 cut(s) 184
Eco130I CCWWGG 3 cut(s) 225, 264, 450
Eco24I GRGCYC 2 cut(s) 144, 186
Eco47I GGWCC 3 cut(s) 166, 196, 430
Eco53kI GAGCTC 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 191
Eco81I CCTNAGG 1 cut(s) 417
EcoICRI GAGCTC 1 cut(s) 184
EcoRI GAATTC 2 cut(s) 347, 367
EcoRII CCWGG 1 cut(s) 310
EcoT14I CCWWGG 3 cut(s) 225, 264, 450
EcoT38I GRGCYC 2 cut(s) 144, 186
ErhI CCWWGG 3 cut(s) 225, 264, 450
FaeI CATG 2 cut(s) 268, 467
FaiI YATR 6 cut(s) 66, 266, 329, 393, 398, 465
FatI CATG 2 cut(s) 264, 463
FauI CCCGC 1 cut(s) 151
FriOI GRGCYC 2 cut(s) 144, 186
GsuI CTGGAG 1 cut(s) 294
HaeIII GGCC 3 cut(s) 59, 80, 230
HapII CCGG 2 cut(s) 60, 487
HgaI GACGC 1 cut(s) 269
Hin1I GRCGYC 1 cut(s) 261
Hin1II CATG 2 cut(s) 268, 467
HindIII AAGCTT 1 cut(s) 69
HinfI GANTC 1 cut(s) 315
HpaII CCGG 2 cut(s) 60, 487
HphI GGTGA 1 cut(s) 214
Hpy166II GTNNAC 2 cut(s) 235, 430
Hpy188I TCNGA 1 cut(s) 323
Hpy188III TCNNGA 1 cut(s) 187
Hpy8I GTNNAC 2 cut(s) 235, 430
Hpy99I CGWCG 1 cut(s) 129
HpyAV CCTTC 3 cut(s) 184, 275, 463
HpyCH4III ACNGT 2 cut(s) 244, 402
HpyCH4IV ACGT 1 cut(s) 353
HpyCH4V TGCA 2 cut(s) 218, 405
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 109, 148, 305
HpyF3I CTNAG 2 cut(s) 7, 417
HpySE526I ACGT 1 cut(s) 353
Hsp92I GRCGYC 1 cut(s) 261
Hsp92II CATG 2 cut(s) 268, 467
KroI GCCGGC 1 cut(s) 59
KroNI GCCGGC 1 cut(s) 61
LmnI GCTCC 4 cut(s) 139, 181, 313, 387
MaeII ACGT 1 cut(s) 353
MaeIII GTNAC 1 cut(s) 292
MboII GAAGA 3 cut(s) 28, 141, 184
MhlI GDGCHC 2 cut(s) 144, 186
MluCI AATT 7 cut(s) 22, 34, 51, 347, 367, 378, 481
MnlI CCTC 2 cut(s) 187, 412
MroNI GCCGGC 1 cut(s) 59
MseI TTAA 1 cut(s) 357
MspI CCGG 2 cut(s) 60, 487
MspR9I CCNGG 1 cut(s) 312
Mva1269I GAATGC 1 cut(s) 218
MvaI CCWGG 1 cut(s) 312
MwoI GCNNNNNNNGC 4 cut(s) 77, 109, 148, 305
NaeI GCCGGC 1 cut(s) 61
NcoI CCATGG 1 cut(s) 264
NgoMIV GCCGGC 1 cut(s) 59
NlaIII CATG 2 cut(s) 268, 467
NlaIV GGNNCC 2 cut(s) 141, 499
NmuCI GTSAC 1 cut(s) 292
PctI GAATGC 1 cut(s) 218
PdiI GCCGGC 1 cut(s) 61
PfeI GAWTC 1 cut(s) 315
PflFI GACNNNGTC 1 cut(s) 341
PfoI TCCNGGA 1 cut(s) 310
Psp124BI GAGCTC 1 cut(s) 186
Psp6I CCWGG 1 cut(s) 310
PspGI CCWGG 1 cut(s) 310
PspN4I GGNNCC 2 cut(s) 141, 499
PspPI GGNCC 4 cut(s) 57, 166, 196, 430
PsyI GACNNNGTC 1 cut(s) 341
RsaI GTAC 1 cut(s) 505
RsaNI GTAC 1 cut(s) 504
SacI GAGCTC 1 cut(s) 186
SaqAI TTAA 1 cut(s) 357
Sau96I GGNCC 4 cut(s) 57, 166, 196, 430
ScrFI CCNGG 1 cut(s) 312
SduI GDGCHC 2 cut(s) 144, 186
SinI GGWCC 3 cut(s) 166, 196, 430
Sse9I AATT 7 cut(s) 22, 34, 51, 347, 367, 378, 481
SsiI CCGC 1 cut(s) 144
SstI GAGCTC 1 cut(s) 186
StyD4I CCNGG 1 cut(s) 310
StyI CCWWGG 3 cut(s) 225, 264, 450
TaaI ACNGT 2 cut(s) 244, 402
TaiI ACGT 1 cut(s) 356
TaqI TCGA 3 cut(s) 26, 204, 345
TasI AATT 7 cut(s) 22, 34, 51, 347, 367, 378, 481
TfiI GAWTC 1 cut(s) 315
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TscAI CASTG 1 cut(s) 407
TseFI GTSAC 1 cut(s) 292
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 2 cut(s) 81, 307
TspRI CASTG 1 cut(s) 407
Tth111I GACNNNGTC 1 cut(s) 341
VpaK11BI GGWCC 3 cut(s) 166, 196, 430
XapI RAATTY 3 cut(s) 34, 347, 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.