pycom16g02270

Sulfate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005511
Physical Location & Seq
Forward (+)
1460119 .. 1463095
2977 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1188 bp
ATGGGAGAAGTCTCCCATGATAAAAGAGAGGATTGCTGCTCTCCCACAAGGATTAACATCCAGGAGCAGCAGTCCACCACGGACATGCAGCAGCAGCAGCACCATCACTGCATGGAAATCCCAATGGGGATGGAGTTGCACAAAGTAGTTGCCCCGCCTTACAGAAGCACTTTTCGTAAGCTGCTGGCCAAGCTCAAAGAAACATTCTTTCCAGATGACCCTCTTTACCAATTCAAAGGTCAGCCACCAAAGAAAAAATGGATTCTTGGAGCTCAGTATGTCTTTCCAATCCTTGAGTGGGGCCCGAATTACAGCTTCAAGCTCCTCAAATCAGACATCATTTCTGGTGTCACCATCGCCAGTTTAGCCATCCCTCAGGGAATCAGTTATGCTAAGCTTGCGAATCTACCTCCGATTGTGGGTCTCTATTCCAGCTTCGTTCCTCCTTTGCTGTATGCTGTCCTTGGAAGCTCAAAGGATCTTGCAGTAGGACCTGTTTCCATTGCTTCCCTAATCATGGGATCGATGCTCATGCAAGAAGTATCTCCCACCAAAGAACCCTACTTGTTCCTCCAACTTGCCTTCACTTCAACCTTCTTTAGTGGCATCCTCCAAGCTTCCCTAGGTTTATTAAGGCTTGGATTTATAATCGATTTTCTTTCAAAGGCTACTCTACTTGGTTTCATGGCCGGAGCTGCTGTGATAGTCTCTCTACAACAGCTTAAGAGTCTCCTTGGAATCACACATTTCACCAAGAAAATGGCAATCATCCCTGTTCTTAGCTCTGTTTTTCATGAAAGAAAGGAGTGGTCATGGCAAACCATTGTAATGGGCGTTTGCTTCCTTGTGCTGCTCCTAATCGCAAGACACGTTAGCATAAAGAAGCCAAAGCTGTTCTGGGTCTCAGCTGGAGCCCCTCTGGTGTCTGTGATCATTTCCACTGTGATTGTCTTTGCATTCAAAGCCGATCGCCATGGCATCAGTGTGATTGGAGAATTAGAGAAAGGACTGAACCCACCATCATGGAACATGTTGGTTTTCCATGGATCCCACCTTGCACTTGCAATCAAGACTGGGATTATCACTGGCATTATTGCCCTCACTGAAGGTATTGCAGTAGGAAGAACTTTTGCTAATATAAGAGAATACAGAGTGGATGGAAACAAGGAGATGATAGCAATTGGATGA

Protein Analysis

396

Amino Acids

43.57

Weight (kDa)

9.41

Isoelectric Point (pI)

29.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sulfate_transp PF00916 109 - 395 3.6e-95 Sulfate permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 647
AciI CCGC 1 cut(s) 155
AclWI GGATC 4 cut(s) 486, 529, 1041, 1054
AcoI YGGCCR 2 cut(s) 186, 687
AcuI CTGAAG 1 cut(s) 1125
AfiI CCNNNNNNNGG 3 cut(s) 298, 419, 517
AflII CTTAAG 1 cut(s) 722
AflIII ACRYGT 2 cut(s) 868, 1029
AgsI TTSAA 5 cut(s) 235, 319, 591, 663, 961
AjnI CCWGG 1 cut(s) 60
Alw21I GWGCWC 1 cut(s) 274
Alw26I GTCTC 5 cut(s) 16, 428, 712, 734, 907
AlwI GGATC 4 cut(s) 486, 529, 1041, 1054
AoxI GGCC 3 cut(s) 186, 301, 687
ApaI GGGCCC 1 cut(s) 305
ApeKI GCWGC 9 cut(s) 36, 67, 88, 91, 94, 97, 181, 695, 850
AspA2I CCTAGG 1 cut(s) 622
AspS9I GGNCC 3 cut(s) 301, 302, 491
AsuHPI GGTGA 2 cut(s) 343, 742
AvaII GGWCC 1 cut(s) 491
AvrII CCTAGG 1 cut(s) 622
AxyI CCTNAGG 1 cut(s) 375
BaeGI GKGCMC 1 cut(s) 305
BalI TGGCCA 1 cut(s) 188
BamHI GGATCC 1 cut(s) 1046
BanII GRGCYC 3 cut(s) 274, 305, 916
Bbv12I GWGCWC 1 cut(s) 274
BbvI GCAGC 9 cut(s) 23, 79, 100, 103, 106, 109, 168, 682, 837
BccI CCATC 6 cut(s) 111, 124, 362, 377, 1027, 1151
BcgI CGANNNNNNTGC 2 cut(s) 514, 548
BciT130I CCWGG 1 cut(s) 62
BclI TGATCA 1 cut(s) 930
BcoDI GTCTC 5 cut(s) 16, 428, 712, 734, 907
BfaI CTAG 1 cut(s) 623
BfrI CTTAAG 1 cut(s) 722
BisI GCNGC 9 cut(s) 37, 68, 89, 92, 95, 98, 182, 696, 851
BlnI CCTAGG 1 cut(s) 622
BlpI GCTNAGC 1 cut(s) 393
BlsI GCNGC 9 cut(s) 38, 69, 90, 93, 96, 99, 183, 697, 852
Bme1390I CCNGG 1 cut(s) 62
Bme18I GGWCC 1 cut(s) 491
BmgT120I GGNCC 3 cut(s) 301, 302, 491
BmiI GGNNCC 4 cut(s) 302, 303, 913, 1048
BmrFI CCNGG 1 cut(s) 62
BmrI ACTGGG 1 cut(s) 1083
BmsI GCATC 3 cut(s) 516, 615, 987
BmuI ACTGGG 1 cut(s) 1083
BpmI CTGGAG 1 cut(s) 930
Bpu1102I GCTNAGC 1 cut(s) 393
BpuEI CTTGAG 1 cut(s) 314
Bsa29I ATCGAT 2 cut(s) 524, 651
BsaBI GATNNNNATC 1 cut(s) 56
BsaI GGTCTC 2 cut(s) 428, 907
BsaJI CCNNGG 6 cut(s) 78, 463, 622, 733, 973, 1042
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 684, 714
Bsc4I CCNNNNNNNGG 3 cut(s) 298, 419, 517
Bse1I ACTGG 3 cut(s) 360, 1078, 1090
Bse21I CCTNAGG 1 cut(s) 375
Bse3DI GCAATG 1 cut(s) 501
Bse8I GATNNNNATC 1 cut(s) 56
BseBI CCWGG 1 cut(s) 62
BseCI ATCGAT 2 cut(s) 524, 651
BseDI CCNNGG 6 cut(s) 78, 463, 622, 733, 973, 1042
BseGI GGATG 6 cut(s) 57, 135, 369, 606, 768, 1162
BseJI GATNNNNATC 1 cut(s) 56
BseLI CCNNNNNNNGG 3 cut(s) 298, 419, 517
BseMI GCAATG 1 cut(s) 501
BseMII CTCAG 3 cut(s) 287, 389, 918
BseNI ACTGG 3 cut(s) 360, 1078, 1090
BseRI GAGGAG 1 cut(s) 314
BseSI GKGCMC 1 cut(s) 305
BseXI GCAGC 9 cut(s) 23, 79, 100, 103, 106, 109, 168, 682, 837
Bsh1285I CGRYCG 1 cut(s) 970
BshFI GGCC 3 cut(s) 188, 303, 689
BshVI ATCGAT 2 cut(s) 524, 651
BsiEI CGRYCG 1 cut(s) 970
BsiHKAI GWGCWC 1 cut(s) 274
BsiSI CCGG 1 cut(s) 690
BslI CCNNNNNNNGG 3 cut(s) 298, 419, 517
BsmAI GTCTC 5 cut(s) 16, 428, 712, 734, 907
BsmI GAATGC 1 cut(s) 956
BsnI GGCC 3 cut(s) 188, 303, 689
Bso31I GGTCTC 2 cut(s) 428, 907
Bsp120I GGGCCC 1 cut(s) 301
Bsp1286I GDGCHC 3 cut(s) 274, 305, 916
Bsp143I GATC 5 cut(s) 478, 521, 930, 967, 1046
Bsp1720I GCTNAGC 1 cut(s) 393
Bsp19I CCATGG 2 cut(s) 973, 1042
BspACI CCGC 1 cut(s) 155
BspANI GGCC 3 cut(s) 188, 303, 689
BspCNI CTCAG 3 cut(s) 286, 388, 917
BspDI ATCGAT 2 cut(s) 524, 651
BspHI TCATGA 1 cut(s) 793
BspLI GGNNCC 4 cut(s) 302, 303, 913, 1048
BspPI GGATC 4 cut(s) 486, 529, 1041, 1054
BspTI CTTAAG 1 cut(s) 722
BspTNI GGTCTC 2 cut(s) 428, 907
BsrDI GCAATG 1 cut(s) 501
BsrI ACTGG 3 cut(s) 360, 1078, 1090
BssECI CCNNGG 6 cut(s) 78, 463, 622, 733, 973, 1042
BssMI GATC 5 cut(s) 478, 521, 930, 967, 1046
BssT1I CCWWGG 5 cut(s) 463, 622, 733, 973, 1042
Bst2UI CCWGG 1 cut(s) 62
Bst4CI ACNGT 1 cut(s) 943
BstAFI CTTAAG 1 cut(s) 722
BstC8I GCNNGC 2 cut(s) 186, 399
BstDEI CTNAG 5 cut(s) 273, 375, 393, 779, 904
BstDSI CCRYGG 3 cut(s) 78, 973, 1042
BstF5I GGATG 6 cut(s) 57, 135, 369, 606, 768, 1162
BstKTI GATC 5 cut(s) 481, 524, 933, 970, 1049
BstMAI GTCTC 5 cut(s) 16, 428, 712, 734, 907
BstMBI GATC 5 cut(s) 478, 521, 930, 967, 1046
BstMCI CGRYCG 1 cut(s) 970
BstMWI GCNNNNNNNGC 7 cut(s) 94, 97, 190, 365, 398, 695, 962
BstNI CCWGG 1 cut(s) 62
BstNSI RCATGY 2 cut(s) 88, 1033
BstSCI CCNGG 1 cut(s) 60
BstSLI GKGCMC 1 cut(s) 305
BstV1I GCAGC 9 cut(s) 23, 79, 100, 103, 106, 109, 168, 682, 837
BstX2I RGATCY 2 cut(s) 478, 1046
BstXI CCANNNNNNTGG 3 cut(s) 760, 829, 1023
BstYI RGATCY 2 cut(s) 478, 1046
Bsu15I ATCGAT 2 cut(s) 524, 651
Bsu36I CCTNAGG 1 cut(s) 375
BsuRI GGCC 3 cut(s) 188, 303, 689
BsuTUI ATCGAT 2 cut(s) 524, 651
BtgI CCRYGG 3 cut(s) 78, 973, 1042
BtgZI GCGATG 1 cut(s) 340
BtsCI GGATG 6 cut(s) 57, 135, 369, 606, 768, 1162
BtsI GCAGTG 1 cut(s) 106
BtsIMutI CAGTG 5 cut(s) 106, 939, 988, 1083, 1101
Cac8I GCNNGC 2 cut(s) 186, 399
CciI TCATGA 1 cut(s) 793
Cfr13I GGNCC 3 cut(s) 301, 302, 491
ClaI ATCGAT 2 cut(s) 524, 651
CspCI CAANNNNNGTGG 2 cut(s) 928, 963
DdeI CTNAG 5 cut(s) 273, 375, 393, 779, 904
DpnI GATC 5 cut(s) 480, 523, 932, 969, 1048
DpnII GATC 5 cut(s) 478, 521, 930, 967, 1046
EaeI YGGCCR 2 cut(s) 186, 687
Ecl136II GAGCTC 1 cut(s) 272
Eco130I CCWWGG 5 cut(s) 463, 622, 733, 973, 1042
Eco24I GRGCYC 3 cut(s) 274, 305, 916
Eco31I GGTCTC 2 cut(s) 428, 907
Eco47I GGWCC 1 cut(s) 491
Eco53kI GAGCTC 1 cut(s) 272
Eco57I CTGAAG 1 cut(s) 1125
Eco81I CCTNAGG 1 cut(s) 375
EcoICRI GAGCTC 1 cut(s) 272
EcoO109I RGGNCCY 2 cut(s) 301, 491
EcoRII CCWGG 1 cut(s) 60
EcoT14I CCWWGG 5 cut(s) 463, 622, 733, 973, 1042
EcoT38I GRGCYC 3 cut(s) 274, 305, 916
ErhI CCWWGG 5 cut(s) 463, 622, 733, 973, 1042
FauI CCCGC 1 cut(s) 162
FbaI TGATCA 1 cut(s) 930
Fnu4HI GCNGC 9 cut(s) 37, 68, 89, 92, 95, 98, 182, 696, 851
FokI GGATG 6 cut(s) 44, 142, 356, 593, 755, 1169
FriOI GRGCYC 3 cut(s) 274, 305, 916
Fsp4HI GCNGC 9 cut(s) 37, 68, 89, 92, 95, 98, 182, 696, 851
FspBI CTAG 1 cut(s) 623
GluI GCNGC 9 cut(s) 37, 68, 89, 92, 95, 98, 182, 696, 851
GsuI CTGGAG 1 cut(s) 930
HaeIII GGCC 3 cut(s) 188, 303, 689
HapII CCGG 1 cut(s) 690
HindIII AAGCTT 2 cut(s) 395, 615
HinfI GANTC 5 cut(s) 262, 381, 403, 727, 738
HpaII CCGG 1 cut(s) 690
HphI GGTGA 2 cut(s) 343, 742
Hpy166II GTNNAC 1 cut(s) 75
Hpy188I TCNGA 2 cut(s) 334, 414
Hpy188III TCNNGA 3 cut(s) 212, 794, 1069
Hpy8I GTNNAC 1 cut(s) 75
HpyAV CCTTC 3 cut(s) 592, 604, 1100
HpyCH4III ACNGT 1 cut(s) 943
HpyCH4IV ACGT 1 cut(s) 870
HpyCH4V TGCA 9 cut(s) 88, 111, 139, 485, 535, 956, 1058, 1064, 1115
HpyF10VI GCNNNNNNNGC 7 cut(s) 94, 97, 190, 365, 398, 695, 962
HpyF3I CTNAG 5 cut(s) 273, 375, 393, 779, 904
HpySE526I ACGT 1 cut(s) 870
Ksp22I TGATCA 1 cut(s) 930
Kzo9I GATC 5 cut(s) 478, 521, 930, 967, 1046
LmnI GCTCC 6 cut(s) 64, 269, 327, 692, 858, 911
Lsp1109I GCAGC 9 cut(s) 23, 79, 100, 103, 106, 109, 168, 682, 837
LweI GCATC 3 cut(s) 516, 615, 987
MaeI CTAG 1 cut(s) 623
MaeII ACGT 1 cut(s) 870
MaeIII GTNAC 1 cut(s) 349
MalI GATC 5 cut(s) 480, 523, 932, 969, 1048
MboI GATC 5 cut(s) 478, 521, 930, 967, 1046
MboII GAAGA 1 cut(s) 1134
MfeI CAATTG 1 cut(s) 1179
MflI RGATCY 2 cut(s) 478, 1046
MhlI GDGCHC 3 cut(s) 274, 305, 916
MlsI TGGCCA 1 cut(s) 188
MluCI AATT 4 cut(s) 230, 307, 995, 1179
MluNI TGGCCA 1 cut(s) 188
MlyI GAGTC 1 cut(s) 736
MmeI TCCRAC 1 cut(s) 598
Mox20I TGGCCA 1 cut(s) 188
MscI TGGCCA 1 cut(s) 188
MseI TTAA 3 cut(s) 54, 632, 723
MslI CAYNNNNRTG 4 cut(s) 83, 827, 983, 1021
Msp20I TGGCCA 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 908
MspCI CTTAAG 1 cut(s) 722
MspI CCGG 1 cut(s) 690
MspR9I CCNGG 1 cut(s) 62
MunI CAATTG 1 cut(s) 1179
Mva1269I GAATGC 1 cut(s) 956
MvaI CCWGG 1 cut(s) 62
MwoI GCNNNNNNNGC 7 cut(s) 94, 97, 190, 365, 398, 695, 962
NcoI CCATGG 2 cut(s) 973, 1042
NdeII GATC 5 cut(s) 478, 521, 930, 967, 1046
NlaIV GGNNCC 4 cut(s) 302, 303, 913, 1048
NmuCI GTSAC 1 cut(s) 349
NspI RCATGY 2 cut(s) 88, 1033
PagI TCATGA 1 cut(s) 793
PciI ACATGT 1 cut(s) 1029
PcsI WCGNNNNNNNCGW 1 cut(s) 867
PctI GAATGC 1 cut(s) 956
PfeI GAWTC 4 cut(s) 262, 381, 403, 738
PfoI TCCNGGA 1 cut(s) 60
PkrI GCNGC 9 cut(s) 38, 69, 90, 93, 96, 99, 183, 697, 852
Ple19I CGATCG 1 cut(s) 970
PleI GAGTC 1 cut(s) 735
PpsI GAGTC 1 cut(s) 735
PpuMI RGGWCCY 1 cut(s) 491
PscI ACATGT 1 cut(s) 1029
PsiI TTATAA 1 cut(s) 647
Psp124BI GAGCTC 1 cut(s) 274
Psp5II RGGWCCY 1 cut(s) 491
Psp6I CCWGG 1 cut(s) 60
PspGI CCWGG 1 cut(s) 60
PspN4I GGNNCC 4 cut(s) 302, 303, 913, 1048
PspOMI GGGCCC 1 cut(s) 301
PspPI GGNCC 3 cut(s) 301, 302, 491
PspPPI RGGWCCY 1 cut(s) 491
PsuI RGATCY 2 cut(s) 478, 1046
PvuI CGATCG 1 cut(s) 970
PvuII CAGCTG 1 cut(s) 908
RseI CAYNNNNRTG 4 cut(s) 83, 827, 983, 1021
SacI GAGCTC 1 cut(s) 274
SaqAI TTAA 3 cut(s) 54, 632, 723
SatI GCNGC 9 cut(s) 37, 68, 89, 92, 95, 98, 182, 696, 851
Sau3AI GATC 5 cut(s) 478, 521, 930, 967, 1046
Sau96I GGNCC 3 cut(s) 301, 302, 491
SchI GAGTC 1 cut(s) 736
ScrFI CCNGG 1 cut(s) 62
SduI GDGCHC 3 cut(s) 274, 305, 916
SfaNI GCATC 3 cut(s) 516, 615, 987
SinI GGWCC 1 cut(s) 491
SmiMI CAYNNNNRTG 4 cut(s) 83, 827, 983, 1021
SmlI CTYRAG 2 cut(s) 293, 722
SmoI CTYRAG 2 cut(s) 293, 722
Sse9I AATT 4 cut(s) 230, 307, 995, 1179
SsiI CCGC 1 cut(s) 155
SspMI CTAG 1 cut(s) 623
SstI GAGCTC 1 cut(s) 274
StyD4I CCNGG 1 cut(s) 60
StyI CCWWGG 5 cut(s) 463, 622, 733, 973, 1042
TaaI ACNGT 1 cut(s) 943
TaiI ACGT 1 cut(s) 873
TaqI TCGA 2 cut(s) 524, 651
TasI AATT 4 cut(s) 230, 307, 995, 1179
TfiI GAWTC 4 cut(s) 262, 381, 403, 738
Tru1I TTAA 3 cut(s) 54, 632, 723
Tru9I TTAA 3 cut(s) 54, 632, 723
TscAI CASTG 5 cut(s) 113, 946, 988, 1090, 1108
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 9 cut(s) 36, 67, 88, 91, 94, 97, 181, 695, 850
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 3 cut(s) 673, 782, 810
TspGWI ACGGA 1 cut(s) 95
TspRI CASTG 5 cut(s) 113, 946, 988, 1090, 1108
Vha464I CTTAAG 1 cut(s) 722
VpaK11BI GGWCC 1 cut(s) 491
XceI RCATGY 2 cut(s) 88, 1033
XcmI CCANNNNNNNNNTGG 3 cut(s) 255, 294, 894
XmaJI CCTAGG 1 cut(s) 622
XspI CTAG 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.