pycom16g03290

Protein of unknown function (DUF789)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0024163
Physical Location & Seq
Reverse (-)
2052606 .. 2053126
521 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g03290.2

Sequence Viewer

Length: 441 bp
ATGTTGAGTACGGGATTGCAGTTTACGCGCGGCCGCGGAGAGGATCGATTTTACAATCCGGCAAGAGCTCGTAGGGCCCACCAGAACCAGAAGGCGGAGCAACTTCGCAGACCTCAGAGCGATGTCACGCCCAGCCCGTCTCCTTCCTTGAAGGGTAACCCCAACCGGGAGCCGGAGAACCGGACCGGGTCCGATGAGCCGCCCAAGCCCGTCGCCGTGCCTGCTTTTGAGCCCGTCGTGGAGCCCTTGAGTAATCTCGAGCGGTTCTTGCAGTCGATCGATCTCTCCGTCCGTCCCCGCCCAGTACCTCTCCAAGAGGACGATGAGAGGATTGAGTACATGCGACGTGGAATATCAGCCATACTTTGTTCTAGGGGATTTATGGGAGTCCTTCAAGGAGTGGAGTGCTTATGGTGCAGGAGTGCCTCTGATTTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF789

Protein Analysis

147

Amino Acids

16.52

Weight (kDa)

7.73

Isoelectric Point (pI)

83.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024163)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g37821
pyrus_communis pycom16g03290 pycom17g21180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 262
AccII CGCG 3 cut(s) 28, 30, 36
AciI CCGC 7 cut(s) 30, 34, 36, 95, 200, 262, 298
AclWI GGATC 1 cut(s) 51
AcoI YGGCCR 1 cut(s) 31
AfaI GTAC 3 cut(s) 10, 306, 338
AfiI CCNNNNNNNGG 4 cut(s) 40, 94, 166, 172
AgsI TTSAA 3 cut(s) 151, 395, 437
AjiI CACGTC 1 cut(s) 347
AluBI AGCT 1 cut(s) 68
AluI AGCT 1 cut(s) 68
Alw21I GWGCWC 1 cut(s) 70
Alw26I GTCTC 1 cut(s) 144
AlwI GGATC 1 cut(s) 51
Ama87I CYCGRG 1 cut(s) 257
AoxI GGCC 2 cut(s) 31, 75
ApaI GGGCCC 1 cut(s) 79
AspLEI GCGC 1 cut(s) 30
AspS9I GGNCC 4 cut(s) 75, 76, 183, 189
AsuC2I CCSGG 2 cut(s) 167, 187
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 2 cut(s) 183, 189
BaeGI GKGCMC 1 cut(s) 79
BanII GRGCYC 4 cut(s) 70, 79, 234, 246
Bbv12I GWGCWC 1 cut(s) 70
BceAI ACGGC 1 cut(s) 200
BcnI CCSGG 2 cut(s) 167, 187
BcoDI GTCTC 1 cut(s) 144
BfaI CTAG 1 cut(s) 372
BisI GCNGC 3 cut(s) 31, 34, 200
BlsI GCNGC 3 cut(s) 32, 35, 201
Bme1390I CCNGG 2 cut(s) 167, 187
Bme18I GGWCC 2 cut(s) 183, 189
BmeT110I CYCGRG 1 cut(s) 257
BmgBI CACGTC 1 cut(s) 347
BmgT120I GGNCC 4 cut(s) 75, 76, 183, 189
BmiI GGNNCC 4 cut(s) 77, 171, 190, 243
BmrFI CCNGG 2 cut(s) 167, 187
BmrI ACTGGG 1 cut(s) 296
BmuI ACTGGG 1 cut(s) 296
BpuEI CTTGAG 1 cut(s) 268
BpuMI CCSGG 2 cut(s) 167, 187
Bsa29I ATCGAT 2 cut(s) 46, 279
BsaJI CCNNGG 1 cut(s) 34
BsaWI WCCGGW 1 cut(s) 180
Bsc4I CCNNNNNNNGG 4 cut(s) 40, 94, 166, 172
Bse1I ACTGG 1 cut(s) 302
BseCI ATCGAT 2 cut(s) 46, 279
BseDI CCNNGG 1 cut(s) 34
BseLI CCNNNNNNNGG 4 cut(s) 40, 94, 166, 172
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 1 cut(s) 302
BseSI GKGCMC 1 cut(s) 79
BseX3I CGGCCG 1 cut(s) 31
BseYI CCCAGC 1 cut(s) 131
BsgI GTGCAG 1 cut(s) 436
Bsh1236I CGCG 3 cut(s) 28, 30, 36
Bsh1285I CGRYCG 2 cut(s) 34, 279
BshFI GGCC 2 cut(s) 33, 77
BshVI ATCGAT 2 cut(s) 46, 279
BsiEI CGRYCG 2 cut(s) 34, 279
BsiHKAI GWGCWC 1 cut(s) 70
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 5 cut(s) 59, 166, 173, 181, 186
BslFI GGGAC 1 cut(s) 279
BslI CCNNNNNNNGG 4 cut(s) 40, 94, 166, 172
BsmAI GTCTC 1 cut(s) 144
BsmBI CGTCTC 1 cut(s) 144
BsmFI GGGAC 1 cut(s) 279
BsnI GGCC 2 cut(s) 33, 77
BsoBI CYCGRG 1 cut(s) 257
Bsp120I GGGCCC 1 cut(s) 75
Bsp1286I GDGCHC 4 cut(s) 70, 79, 234, 246
Bsp143I GATC 3 cut(s) 43, 276, 280
BspACI CCGC 7 cut(s) 30, 34, 36, 95, 200, 262, 298
BspANI GGCC 2 cut(s) 33, 77
BspCNI CTCAG 1 cut(s) 127
BspDI ATCGAT 2 cut(s) 46, 279
BspFNI CGCG 3 cut(s) 28, 30, 36
BspLI GGNNCC 4 cut(s) 77, 171, 190, 243
BspPI GGATC 1 cut(s) 51
BsrBI CCGCTC 1 cut(s) 262
BsrI ACTGG 1 cut(s) 302
BssECI CCNNGG 1 cut(s) 34
BssMI GATC 3 cut(s) 43, 276, 280
BstC8I GCNNGC 1 cut(s) 222
BstDEI CTNAG 1 cut(s) 114
BstDSI CCRYGG 1 cut(s) 34
BstEII GGTNACC 1 cut(s) 155
BstFNI CGCG 3 cut(s) 28, 30, 36
BstHHI GCGC 1 cut(s) 30
BstKTI GATC 3 cut(s) 46, 279, 283
BstMAI GTCTC 1 cut(s) 144
BstMBI GATC 3 cut(s) 43, 276, 280
BstMCI CGRYCG 2 cut(s) 34, 279
BstMWI GCNNNNNNNGC 6 cut(s) 25, 74, 205, 221, 268, 414
BstNSI RCATGY 1 cut(s) 343
BstPI GGTNACC 1 cut(s) 155
BstSCI CCNGG 2 cut(s) 165, 185
BstSLI GKGCMC 1 cut(s) 79
BstUI CGCG 3 cut(s) 28, 30, 36
BstZI CGGCCG 1 cut(s) 31
Bsu15I ATCGAT 2 cut(s) 46, 279
BsuRI GGCC 2 cut(s) 33, 77
BsuTUI ATCGAT 2 cut(s) 46, 279
BtgI CCRYGG 1 cut(s) 34
BtgZI GCGATG 1 cut(s) 135
BtrI CACGTC 1 cut(s) 347
Cac8I GCNNGC 1 cut(s) 222
CciNI GCGGCCGC 1 cut(s) 31
CfoI GCGC 1 cut(s) 30
Cfr13I GGNCC 4 cut(s) 75, 76, 183, 189
Cfr42I CCGCGG 1 cut(s) 37
ClaI ATCGAT 2 cut(s) 46, 279
CpoI CGGWCCG 1 cut(s) 183
Csp6I GTAC 3 cut(s) 9, 305, 337
CspI CGGWCCG 1 cut(s) 183
CviAII CATG 1 cut(s) 340
CviQI GTAC 3 cut(s) 9, 305, 337
DdeI CTNAG 1 cut(s) 114
DpnI GATC 3 cut(s) 45, 278, 282
DpnII GATC 3 cut(s) 43, 276, 280
EaeI YGGCCR 1 cut(s) 31
EagI CGGCCG 1 cut(s) 31
EciI GGCGGA 1 cut(s) 110
Ecl136II GAGCTC 1 cut(s) 68
EclXI CGGCCG 1 cut(s) 31
Eco24I GRGCYC 4 cut(s) 70, 79, 234, 246
Eco47I GGWCC 2 cut(s) 183, 189
Eco52I CGGCCG 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 68
Eco88I CYCGRG 1 cut(s) 257
Eco91I GGTNACC 1 cut(s) 155
EcoICRI GAGCTC 1 cut(s) 68
EcoO109I RGGNCCY 1 cut(s) 75
EcoO65I GGTNACC 1 cut(s) 155
EcoT38I GRGCYC 4 cut(s) 70, 79, 234, 246
Esp3I CGTCTC 1 cut(s) 144
FaeI CATG 1 cut(s) 343
FaiI YATR 4 cut(s) 341, 362, 383, 412
FaqI GGGAC 1 cut(s) 279
FatI CATG 1 cut(s) 339
FauI CCCGC 1 cut(s) 305
Fnu4HI GCNGC 3 cut(s) 31, 34, 200
FriOI GRGCYC 4 cut(s) 70, 79, 234, 246
Fsp4HI GCNGC 3 cut(s) 31, 34, 200
FspBI CTAG 1 cut(s) 372
GlaI GCGC 1 cut(s) 29
GluI GCNGC 3 cut(s) 31, 34, 200
GsaI CCCAGC 1 cut(s) 135
HaeIII GGCC 2 cut(s) 33, 77
HapII CCGG 5 cut(s) 59, 166, 173, 181, 186
HhaI GCGC 1 cut(s) 30
Hin1II CATG 1 cut(s) 343
Hin6I GCGC 1 cut(s) 28
HinP1I GCGC 1 cut(s) 28
HinfI GANTC 1 cut(s) 387
HpaII CCGG 5 cut(s) 59, 166, 173, 181, 186
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 3 cut(s) 117, 193, 430
Hpy188III TCNNGA 1 cut(s) 257
Hpy8I GTNNAC 1 cut(s) 24
Hpy99I CGWCG 3 cut(s) 215, 239, 348
HpyAV CCTTC 4 cut(s) 85, 145, 153, 401
HpyCH4IV ACGT 1 cut(s) 346
HpyCH4V TGCA 3 cut(s) 19, 271, 417
HpyF10VI GCNNNNNNNGC 6 cut(s) 25, 74, 205, 221, 268, 414
HpyF3I CTNAG 1 cut(s) 114
HpySE526I ACGT 1 cut(s) 346
Hsp92II CATG 1 cut(s) 343
HspAI GCGC 1 cut(s) 28
KspI CCGCGG 1 cut(s) 37
Kzo9I GATC 3 cut(s) 43, 276, 280
LmnI GCTCC 3 cut(s) 97, 169, 241
MaeI CTAG 1 cut(s) 372
MaeII ACGT 1 cut(s) 346
MaeIII GTNAC 2 cut(s) 124, 155
MalI GATC 3 cut(s) 45, 278, 282
MbiI CCGCTC 1 cut(s) 262
MboI GATC 3 cut(s) 43, 276, 280
MhlI GDGCHC 4 cut(s) 70, 79, 234, 246
MlyI GAGTC 1 cut(s) 396
MnlI CCTC 6 cut(s) 34, 123, 310, 318, 321, 436
MspA1I CMGCKG 1 cut(s) 36
MspI CCGG 5 cut(s) 59, 166, 173, 181, 186
MspR9I CCNGG 2 cut(s) 167, 187
MvnI CGCG 3 cut(s) 28, 30, 36
MwoI GCNNNNNNNGC 6 cut(s) 25, 74, 205, 221, 268, 414
NciI CCSGG 2 cut(s) 167, 187
NdeII GATC 3 cut(s) 43, 276, 280
NlaIII CATG 1 cut(s) 343
NlaIV GGNNCC 4 cut(s) 77, 171, 190, 243
NmuCI GTSAC 1 cut(s) 124
NotI GCGGCCGC 1 cut(s) 31
NspI RCATGY 1 cut(s) 343
PaeR7I CTCGAG 1 cut(s) 257
PcsI WCGNNNNNNNCGW 2 cut(s) 134, 285
PflFI GACNNNGTC 1 cut(s) 187
PkrI GCNGC 3 cut(s) 32, 35, 201
Ple19I CGATCG 1 cut(s) 279
PleI GAGTC 1 cut(s) 395
PpsI GAGTC 1 cut(s) 395
Psp124BI GAGCTC 1 cut(s) 70
PspEI GGTNACC 1 cut(s) 155
PspFI CCCAGC 1 cut(s) 131
PspN4I GGNNCC 4 cut(s) 77, 171, 190, 243
PspOMI GGGCCC 1 cut(s) 75
PspPI GGNCC 4 cut(s) 75, 76, 183, 189
PsyI GACNNNGTC 1 cut(s) 187
PvuI CGATCG 1 cut(s) 279
RsaI GTAC 3 cut(s) 10, 306, 338
RsaNI GTAC 3 cut(s) 9, 305, 337
Rsr2I CGGWCCG 1 cut(s) 183
RsrII CGGWCCG 1 cut(s) 183
SacI GAGCTC 1 cut(s) 70
SacII CCGCGG 1 cut(s) 37
SatI GCNGC 3 cut(s) 31, 34, 200
Sau3AI GATC 3 cut(s) 43, 276, 280
Sau96I GGNCC 4 cut(s) 75, 76, 183, 189
SchI GAGTC 1 cut(s) 396
ScrFI CCNGG 2 cut(s) 167, 187
SduI GDGCHC 4 cut(s) 70, 79, 234, 246
SetI ASST 4 cut(s) 70, 115, 310, 349
Sfr274I CTCGAG 1 cut(s) 257
Sfr303I CCGCGG 1 cut(s) 37
SgrBI CCGCGG 1 cut(s) 37
SinI GGWCC 2 cut(s) 183, 189
SlaI CTCGAG 1 cut(s) 257
SmlI CTYRAG 2 cut(s) 247, 257
SmoI CTYRAG 2 cut(s) 247, 257
SsiI CCGC 7 cut(s) 30, 34, 36, 95, 200, 262, 298
SspMI CTAG 1 cut(s) 372
SstI GAGCTC 1 cut(s) 70
StyD4I CCNGG 2 cut(s) 165, 185
TaiI ACGT 1 cut(s) 349
TaqI TCGA 4 cut(s) 46, 258, 275, 279
TatI WGTACW 1 cut(s) 336
TauI GCSGC 3 cut(s) 33, 36, 202
TseFI GTSAC 1 cut(s) 124
Tsp45I GTSAC 1 cut(s) 124
TspGWI ACGGA 2 cut(s) 277, 281
Tth111I GACNNNGTC 1 cut(s) 187
VpaK11BI GGWCC 2 cut(s) 183, 189
XceI RCATGY 1 cut(s) 343
XhoI CTCGAG 1 cut(s) 257
XspI CTAG 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.