pycom16g06060

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012524
Physical Location & Seq
Reverse (-)
3875813 .. 3877138
1326 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g06060.1

Sequence Viewer

Length: 1326 bp
ATGTGGGACACAGCTTTAGCGAAAATGAAATCTCTGTTAGAGGAGCAATTCTCCCATATGAATTCTGCAACCCATCTTCTCCTGGTGAAGGATTATGTGACTCTTCTTGGCTCTACTCTCAGACAATATGGGTATGAAGTGGGACCACTTCTTGAAACGCTTGACAAGAGCCGAGAGAAATACCACGAGCTTCTTTTAGAAGAGTGTCGTCAACAAATTGCAAATGTTATTGCCAATGATACATATGAACAGATGTCCTTGAAAAAGGACACCGACTATGAAAGTAATGTCCTGTCATTCAATCTCCAGACATCAGATATAATACCGGCTTTTCCGTACATTGCACCATTCTCCTCTGCAGTACCTGATACCTGCCGAATTGTCAGATCATTCATCAAAGGGTCTGTTGATTACTTGTCTCATGGGGCACATACTAGTTATTATGATGTTGTGAGGAAGTATTTGGACAAACTCTTGATAGACGTGTTAAATGAGGTGATACTTAATACAATTCATAGCGGTAACATTGGTGTCTCTCAAGCCATGCAGATTGCTGCAAACATTTCTGTAGTTGAAAGAGCTTGTGACTATTTTCTTCGTCATGCAGCCCAACTTTGTGGGATCGATCCAATCCGTTCAGTTGAGAGGCCTCAAGCTAGTTTAACTGCTAAGGTGGTTCTAAAGAGTTCAAGGGATGAAGCTTATCTTGCTTTGCTGAATTTGGTAAACACTAAGTTGGATGAGTTCATGTCACTTACAGAGAATATCAGTTGGACTATGGAGGAAATGCCGCAGAATGGAAATGAGTATATGAATGAAGTTGTTCTTTACCTCGATACTCTTATGTCGACAGCACAACAAATTTTACCTTTGGATGCTTTGTACAAGGTCGGGAGTGGGGCTTTCGATCACATTTCTAACACTATAGTGTCGACTCTTCTAAGTGATAGCGTCAAGAGGTTCACCGCTAATGCAGTTATGGGTATCAACAATGATTTGAAGACGTTGGAGAATTTTGCAGACGAGAGGTTTCAGAGTACCGGCTTGAGCGAAATTTACAAAGAAGGTAGTTTTAAAGGTTGCTTGATAGAAGCGCGGCAACTGATAAACCTTTTGTCGAGCAGTCAGCCAGAGAACTTCATGAATCCTGTGATAAGGGAGAAGAACTACAATGCTTTGGATTACAAAAAGGTGGCTAGTATCTGTGAGAAATTGAAGGATTCTGCTGATGGTATCTTCGGGAGCCTTTCAAACAGAAATAATAGGCAAAGTGCTCGTAAGAAATCAATGGATGTGCTTAAGAAAAGATTGAAGGACTTCAACTGA

Protein Analysis

442

Amino Acids

49.77

Weight (kDa)

5.62

Isoelectric Point (pI)

37.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec15_C PF04091 49 - 404 1.1e-101 Exocyst complex subunit Sec15 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012524)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56640
fragaria_vesca FvH4_5g19480 FvH4_5g19480 FvH4_5g19480
malus_domestica MD13G1069100.v1.1 MD16G1069400.v1.1
prunus_persica Prupe.1G277600_v2.0.a1
pyrus_communis pycom13g06190 pycom16g06060
rosa_chinensis RchiOBHm_Chr7g0204061
rosa_laevigata RLG00000003491
rosa_multiflora Rmu_sc0001311.1_g000011
rosa_roxburghii Rroxscaffold_3G00253390
rosa_rugosa Rorug07G0080300
rosa_samantha Rh7AG210300 Rh7BG208900 Rh7CG222700 Rh7DG217000
rosa_wichuraiana Rw7G018310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 380
AccI GTMKAC 2 cut(s) 848, 932
AccII CGCG 1 cut(s) 1096
AciI CCGC 4 cut(s) 519, 791, 966, 1096
AclWI GGATC 2 cut(s) 620, 629
AcsI RAATTY 5 cut(s) 61, 718, 861, 1012, 1053
AfaI GTAC 4 cut(s) 338, 363, 884, 1039
AfiI CCNNNNNNNGG 2 cut(s) 88, 797
AflII CTTAAG 1 cut(s) 1298
AflIII ACRYGT 1 cut(s) 483
AhlI ACTAGT 1 cut(s) 434
AjiI CACGTC 1 cut(s) 484
AjnI CCWGG 1 cut(s) 81
AleI CACNNNNGTG 1 cut(s) 926
AluBI AGCT 5 cut(s) 14, 190, 581, 656, 701
AluI AGCT 5 cut(s) 14, 190, 581, 656, 701
Alw21I GWGCWC 1 cut(s) 1276
Alw26I GTCTC 2 cut(s) 423, 538
AlwI GGATC 2 cut(s) 620, 629
AlwNI CAGNNNCTG 1 cut(s) 365
AoxI GGCC 1 cut(s) 647
ApeKI GCWGC 2 cut(s) 554, 605
ApoI RAATTY 5 cut(s) 61, 718, 861, 1012, 1053
Asp700I GAANNNNTTC 2 cut(s) 822, 1316
AspLEI GCGC 1 cut(s) 1096
AspS9I GGNCC 1 cut(s) 143
AsuHPI GGTGA 3 cut(s) 97, 508, 955
AvaII GGWCC 1 cut(s) 143
BaeGI GKGCMC 1 cut(s) 430
BauI CACGAG 1 cut(s) 185
BbsI GAAGAC 1 cut(s) 1007
Bbv12I GWGCWC 1 cut(s) 1276
BbvI GCAGC 2 cut(s) 541, 617
BccI CCATC 2 cut(s) 81, 1223
BcgI CGANNNNNNTGC 2 cut(s) 1256, 1290
BciT130I CCWGG 1 cut(s) 83
BcoDI GTCTC 2 cut(s) 423, 538
BcuI ACTAGT 1 cut(s) 434
BfaI CTAG 3 cut(s) 435, 657, 1197
BfmI CTRYAG 3 cut(s) 357, 567, 924
BfrI CTTAAG 1 cut(s) 1298
BfuAI ACCTGC 1 cut(s) 380
BisI GCNGC 4 cut(s) 555, 606, 791, 1097
BlsI GCNGC 4 cut(s) 556, 607, 792, 1098
Bme1390I CCNGG 1 cut(s) 83
Bme18I GGWCC 1 cut(s) 143
BmgBI CACGTC 1 cut(s) 484
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 2 cut(s) 144, 1244
BmrFI CCNGG 1 cut(s) 83
BmsI GCATC 1 cut(s) 865
BpiI GAAGAC 1 cut(s) 1007
BplI GAGNNNNNCTC 2 cut(s) 35, 67
BpmI CTGGAG 1 cut(s) 290
Bpu10I CCTNAGC 1 cut(s) 669
BpuEI CTTGAG 3 cut(s) 522, 636, 1066
Bsa29I ATCGAT 1 cut(s) 624
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 797
Bse118I RCCGGY 2 cut(s) 325, 1040
Bse3DI GCAATG 1 cut(s) 339
BseBI CCWGG 1 cut(s) 83
BseCI ATCGAT 1 cut(s) 624
BseGI GGATG 4 cut(s) 700, 745, 880, 1297
BseLI CCNNNNNNNGG 2 cut(s) 88, 797
BseMI GCAATG 1 cut(s) 339
BseMII CTCAG 1 cut(s) 133
BseRI GAGGAG 2 cut(s) 56, 343
BseSI GKGCMC 1 cut(s) 430
BseXI GCAGC 2 cut(s) 541, 617
Bsh1236I CGCG 1 cut(s) 1096
BshFI GGCC 1 cut(s) 649
BshVI ATCGAT 1 cut(s) 624
BsiHKAI GWGCWC 1 cut(s) 1276
BsiSI CCGG 2 cut(s) 326, 1041
BslFI GGGAC 2 cut(s) 20, 156
BslI CCNNNNNNNGG 2 cut(s) 88, 797
BsmAI GTCTC 2 cut(s) 423, 538
BsmFI GGGAC 2 cut(s) 20, 156
BsnI GGCC 1 cut(s) 649
Bsp1286I GDGCHC 2 cut(s) 430, 1276
Bsp1407I TGTACA 1 cut(s) 882
Bsp143I GATC 4 cut(s) 386, 621, 625, 907
BspACI CCGC 4 cut(s) 519, 791, 966, 1096
BspANI GGCC 1 cut(s) 649
BspCNI CTCAG 1 cut(s) 132
BspDI ATCGAT 1 cut(s) 624
BspFNI CGCG 1 cut(s) 1096
BspHI TCATGA 1 cut(s) 1140
BspLI GGNNCC 2 cut(s) 144, 1244
BspMAI CTGCAG 1 cut(s) 361
BspMI ACCTGC 1 cut(s) 380
BspPI GGATC 2 cut(s) 620, 629
BspTI CTTAAG 1 cut(s) 1298
BsrDI GCAATG 1 cut(s) 339
BsrFI RCCGGY 2 cut(s) 325, 1040
BsrGI TGTACA 1 cut(s) 882
BssAI RCCGGY 2 cut(s) 325, 1040
BssMI GATC 4 cut(s) 386, 621, 625, 907
BssSI CACGAG 1 cut(s) 185
Bst2BI CACGAG 1 cut(s) 185
Bst2UI CCWGG 1 cut(s) 83
Bst6I CTCTTC 3 cut(s) 108, 195, 942
BstAFI CTTAAG 1 cut(s) 1298
BstAUI TGTACA 1 cut(s) 882
BstDEI CTNAG 4 cut(s) 119, 669, 732, 941
BstENI CCTNNNNNAGG 1 cut(s) 86
BstF5I GGATG 4 cut(s) 700, 745, 880, 1297
BstFNI CGCG 1 cut(s) 1096
BstHHI GCGC 1 cut(s) 1096
BstKTI GATC 4 cut(s) 389, 624, 628, 910
BstMAI GTCTC 2 cut(s) 423, 538
BstMBI GATC 4 cut(s) 386, 621, 625, 907
BstMWI GCNNNNNNNGC 1 cut(s) 707
BstNI CCWGG 1 cut(s) 83
BstSCI CCNGG 1 cut(s) 81
BstSFI CTRYAG 3 cut(s) 357, 567, 924
BstSLI GKGCMC 1 cut(s) 430
BstUI CGCG 1 cut(s) 1096
BstV1I GCAGC 2 cut(s) 541, 617
BstV2I GAAGAC 1 cut(s) 1007
BstXI CCANNNNNNTGG 1 cut(s) 617
Bsu15I ATCGAT 1 cut(s) 624
BsuRI GGCC 1 cut(s) 649
BsuTUI ATCGAT 1 cut(s) 624
BtrI CACGTC 1 cut(s) 484
BtsCI GGATG 4 cut(s) 700, 745, 880, 1297
BveI ACCTGC 1 cut(s) 380
CaiI CAGNNNCTG 1 cut(s) 365
CciI TCATGA 1 cut(s) 1140
CfoI GCGC 1 cut(s) 1096
Cfr10I RCCGGY 2 cut(s) 325, 1040
Cfr13I GGNCC 1 cut(s) 143
ClaI ATCGAT 1 cut(s) 624
CseI GACGC 1 cut(s) 940
Csp6I GTAC 4 cut(s) 337, 362, 883, 1038
CviAII CATG 5 cut(s) 422, 544, 602, 748, 1141
CviQI GTAC 4 cut(s) 337, 362, 883, 1038
DdeI CTNAG 4 cut(s) 119, 669, 732, 941
DpnI GATC 4 cut(s) 388, 623, 627, 909
DpnII GATC 4 cut(s) 386, 621, 625, 907
DraI TTTAAA 1 cut(s) 1075
Eam1104I CTCTTC 3 cut(s) 108, 195, 942
EarI CTCTTC 3 cut(s) 108, 195, 942
Eco147I AGGCCT 1 cut(s) 649
Eco47I GGWCC 1 cut(s) 143
EcoNI CCTNNNNNAGG 1 cut(s) 86
EcoRI GAATTC 1 cut(s) 61
EcoRII CCWGG 1 cut(s) 81
FaeI CATG 5 cut(s) 425, 547, 605, 751, 1144
FalI AAGNNNNNCTT 4 cut(s) 690, 722, 810, 842
FaqI GGGAC 2 cut(s) 20, 156
FatI CATG 5 cut(s) 421, 543, 601, 747, 1140
FauNDI CATATG 2 cut(s) 57, 244
FblI GTMKAC 2 cut(s) 848, 932
Fnu4HI GCNGC 4 cut(s) 555, 606, 791, 1097
FokI GGATG 4 cut(s) 707, 752, 887, 1304
Fsp4HI GCNGC 4 cut(s) 555, 606, 791, 1097
FspBI CTAG 3 cut(s) 435, 657, 1197
GlaI GCGC 1 cut(s) 1095
GluI GCNGC 4 cut(s) 555, 606, 791, 1097
GsuI CTGGAG 1 cut(s) 290
HaeIII GGCC 1 cut(s) 649
HapII CCGG 2 cut(s) 326, 1041
HgaI GACGC 1 cut(s) 940
HhaI GCGC 1 cut(s) 1096
Hin1II CATG 5 cut(s) 425, 547, 605, 751, 1144
Hin6I GCGC 1 cut(s) 1094
HinP1I GCGC 1 cut(s) 1094
HincII GTYRAC 3 cut(s) 212, 849, 933
HindII GTYRAC 3 cut(s) 212, 849, 933
HindIII AAGCTT 1 cut(s) 699
HinfI GANTC 4 cut(s) 100, 934, 1144, 1220
HpaII CCGG 2 cut(s) 326, 1041
HphI GGTGA 3 cut(s) 97, 508, 955
Hpy166II GTNNAC 5 cut(s) 212, 727, 849, 933, 963
Hpy188I TCNGA 4 cut(s) 122, 316, 386, 1035
Hpy188III TCNNGA 7 cut(s) 152, 307, 475, 892, 955, 1141, 1240
Hpy8I GTNNAC 5 cut(s) 212, 727, 849, 933, 963
HpyAV CCTTC 4 cut(s) 82, 1058, 1210, 1306
HpyCH4IV ACGT 2 cut(s) 483, 1004
HpyCH4V TGCA 9 cut(s) 68, 221, 344, 359, 547, 557, 605, 974, 1019
HpyF10VI GCNNNNNNNGC 1 cut(s) 707
HpyF3I CTNAG 4 cut(s) 119, 669, 732, 941
HpySE526I ACGT 2 cut(s) 483, 1004
Hsp92II CATG 5 cut(s) 425, 547, 605, 751, 1144
HspAI GCGC 1 cut(s) 1094
Kzo9I GATC 4 cut(s) 386, 621, 625, 907
LmnI GCTCC 2 cut(s) 43, 1242
Lsp1109I GCAGC 2 cut(s) 541, 617
LweI GCATC 1 cut(s) 865
MaeI CTAG 3 cut(s) 435, 657, 1197
MaeII ACGT 2 cut(s) 483, 1004
MaeIII GTNAC 4 cut(s) 97, 521, 584, 750
MalI GATC 4 cut(s) 388, 623, 627, 909
MboI GATC 4 cut(s) 386, 621, 625, 907
MboII GAAGA 8 cut(s) 68, 95, 212, 587, 929, 1012, 1174, 1228
MhlI GDGCHC 2 cut(s) 430, 1276
MlyI GAGTC 2 cut(s) 94, 928
MmeI TCCRAC 3 cut(s) 717, 752, 987
MroXI GAANNNNTTC 2 cut(s) 822, 1316
MseI TTAA 5 cut(s) 488, 504, 662, 1074, 1299
MslI CAYNNNNRTG 1 cut(s) 926
MspCI CTTAAG 1 cut(s) 1298
MspI CCGG 2 cut(s) 326, 1041
MspR9I CCNGG 1 cut(s) 83
MvaI CCWGG 1 cut(s) 83
MvnI CGCG 1 cut(s) 1096
MwoI GCNNNNNNNGC 1 cut(s) 707
NdeI CATATG 2 cut(s) 57, 244
NdeII GATC 4 cut(s) 386, 621, 625, 907
NlaIII CATG 5 cut(s) 425, 547, 605, 751, 1144
NlaIV GGNNCC 2 cut(s) 144, 1244
NmeAIII GCCGAG 1 cut(s) 197
NmuCI GTSAC 3 cut(s) 97, 584, 750
OliI CACNNNNGTG 1 cut(s) 926
PagI TCATGA 1 cut(s) 1140
PceI AGGCCT 1 cut(s) 649
PdmI GAANNNNTTC 2 cut(s) 822, 1316
PfeI GAWTC 2 cut(s) 1144, 1220
PkrI GCNGC 4 cut(s) 556, 607, 792, 1098
PleI GAGTC 2 cut(s) 94, 928
PpsI GAGTC 2 cut(s) 94, 928
Psp6I CCWGG 1 cut(s) 81
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 2 cut(s) 144, 1244
PspPI GGNCC 1 cut(s) 143
PstI CTGCAG 1 cut(s) 361
PstNI CAGNNNCTG 1 cut(s) 365
RsaI GTAC 4 cut(s) 338, 363, 884, 1039
RsaNI GTAC 4 cut(s) 337, 362, 883, 1038
RseI CAYNNNNRTG 1 cut(s) 926
SalI GTCGAC 2 cut(s) 847, 931
SaqAI TTAA 5 cut(s) 488, 504, 662, 1074, 1299
SatI GCNGC 4 cut(s) 555, 606, 791, 1097
Sau3AI GATC 4 cut(s) 386, 621, 625, 907
Sau96I GGNCC 1 cut(s) 143
SchI GAGTC 2 cut(s) 94, 928
ScrFI CCNGG 1 cut(s) 83
SduI GDGCHC 2 cut(s) 430, 1276
SfaNI GCATC 1 cut(s) 865
SfcI CTRYAG 3 cut(s) 357, 567, 924
SinI GGWCC 1 cut(s) 143
SmiMI CAYNNNNRTG 1 cut(s) 926
SmlI CTYRAG 4 cut(s) 537, 651, 1045, 1298
SmoI CTYRAG 4 cut(s) 537, 651, 1045, 1298
SpeI ACTAGT 1 cut(s) 434
SseBI AGGCCT 1 cut(s) 649
SsiI CCGC 4 cut(s) 519, 791, 966, 1096
SspMI CTAG 3 cut(s) 435, 657, 1197
StuI AGGCCT 1 cut(s) 649
StyD4I CCNGG 1 cut(s) 81
TaiI ACGT 2 cut(s) 486, 1007
TaqI TCGA 6 cut(s) 624, 834, 848, 906, 932, 1118
TatI WGTACW 1 cut(s) 882
TauI GCSGC 2 cut(s) 793, 1099
TfiI GAWTC 2 cut(s) 1144, 1220
Tru1I TTAA 5 cut(s) 488, 504, 662, 1074, 1299
Tru9I TTAA 5 cut(s) 488, 504, 662, 1074, 1299
TseFI GTSAC 3 cut(s) 97, 584, 750
TseI GCWGC 2 cut(s) 554, 605
Tsp45I GTSAC 3 cut(s) 97, 584, 750
TspGWI ACGGA 2 cut(s) 324, 623
Vha464I CTTAAG 1 cut(s) 1298
VpaK11BI GGWCC 1 cut(s) 143
XagI CCTNNNNNAGG 1 cut(s) 86
XapI RAATTY 5 cut(s) 61, 718, 861, 1012, 1053
XmiI GTMKAC 2 cut(s) 848, 932
XmnI GAANNNNTTC 2 cut(s) 822, 1316
XspI CTAG 3 cut(s) 435, 657, 1197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.