pycom16g10850

ribosomal small subunit assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0020228
Physical Location & Seq
Forward (+)
7486302 .. 7486815
514 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 291 bp
ATGGCTGGTTGTAAACAGGTGCTTCAGAACGATATCGATTTGCTTCACCCACCAGCTGAGCTCGAAAAGAGGAAGCACAAGCTCAAGAGGCTTGTGCAGACGCCCAACTCTTTTTTCATGGTAACTAATATGCCAAGGATGCTTCAGCATAACAACCGTGTTCAGTCACTCGCAAACAGTTGTGGTGTGTGGGAACTGCCAGACAGTGTTGTGCCAGCCTACTGGAGGCCGAGCCAGGCTCACCGAGGGTTGCTCCTTCAGAAGAAAGGGGGACTGATGCTCGTGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

97

Amino Acids

10.97

Weight (kDa)

10.02

Isoelectric Point (pI)

56.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020228)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61111
fragaria_vesca FvH4_1g27030
pyrus_communis pycom16g10850
rosa_samantha Rh4AG290200 Rh4BG295900 Rh4CG305600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 3 cut(s) 8, 128, 242
AcyI GRCGYC 1 cut(s) 101
AfiI CCNNNNNNNGG 1 cut(s) 225
AjnI CCWGG 1 cut(s) 234
AjuI GAANNNNNNNTTGG 2 cut(s) 98, 130
AluBI AGCT 3 cut(s) 56, 61, 82
AluI AGCT 3 cut(s) 56, 61, 82
Alw21I GWGCWC 1 cut(s) 63
AoxI GGCC 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 38, 233
BanII GRGCYC 1 cut(s) 63
BauI CACGAG 1 cut(s) 281
Bbv12I GWGCWC 1 cut(s) 63
BciT130I CCWGG 1 cut(s) 236
BlpI GCTNAGC 1 cut(s) 57
Bme1390I CCNGG 1 cut(s) 236
BmrFI CCNGG 1 cut(s) 236
BmsI GCATC 2 cut(s) 129, 267
BplI GAGNNNNNCTC 4 cut(s) 223, 255, 237, 269
BpmI CTGGAG 1 cut(s) 244
Bpu1102I GCTNAGC 1 cut(s) 57
BpuEI CTTGAG 1 cut(s) 68
Bsa29I ATCGAT 1 cut(s) 36
BsaHI GRCGYC 1 cut(s) 101
BsaJI CCNNGG 2 cut(s) 134, 244
Bsc4I CCNNNNNNNGG 1 cut(s) 225
Bse1I ACTGG 1 cut(s) 227
BseBI CCWGG 1 cut(s) 236
BseCI ATCGAT 1 cut(s) 36
BseDI CCNNGG 2 cut(s) 134, 244
BseGI GGATG 1 cut(s) 144
BseLI CCNNNNNNNGG 1 cut(s) 225
BseMII CTCAG 1 cut(s) 48
BseNI ACTGG 1 cut(s) 227
BsgI GTGCAG 1 cut(s) 116
BshFI GGCC 1 cut(s) 229
BshVI ATCGAT 1 cut(s) 36
BsiHKAI GWGCWC 1 cut(s) 63
BslFI GGGAC 1 cut(s) 285
BslI CCNNNNNNNGG 1 cut(s) 225
BsmFI GGGAC 1 cut(s) 285
BsnI GGCC 1 cut(s) 229
Bsp1286I GDGCHC 1 cut(s) 63
Bsp1720I GCTNAGC 1 cut(s) 57
BspANI GGCC 1 cut(s) 229
BspCNI CTCAG 1 cut(s) 49
BspDI ATCGAT 1 cut(s) 36
BsrI ACTGG 1 cut(s) 227
BssECI CCNNGG 2 cut(s) 134, 244
BssNI GRCGYC 1 cut(s) 101
BssSI CACGAG 1 cut(s) 281
BssT1I CCWWGG 1 cut(s) 134
Bst2BI CACGAG 1 cut(s) 281
Bst2UI CCWGG 1 cut(s) 236
Bst4CI ACNGT 3 cut(s) 158, 179, 206
BstACI GRCGYC 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 216
BstDEI CTNAG 1 cut(s) 57
BstENI CCTNNNNNAGG 1 cut(s) 223
BstF5I GGATG 1 cut(s) 144
BstMWI GCNNNNNNNGC 2 cut(s) 88, 139
BstNI CCWGG 1 cut(s) 236
BstSCI CCNGG 1 cut(s) 234
BstXI CCANNNNNNTGG 1 cut(s) 222
Bsu15I ATCGAT 1 cut(s) 36
BsuRI GGCC 1 cut(s) 229
BsuTUI ATCGAT 1 cut(s) 36
BtsCI GGATG 1 cut(s) 144
BtsIMutI CAGTG 1 cut(s) 211
Cac8I GCNNGC 1 cut(s) 216
ClaI ATCGAT 1 cut(s) 36
CseI GACGC 1 cut(s) 109
CviAII CATG 1 cut(s) 118
CviJI RGCY 9 cut(s) 5, 56, 61, 82, 91, 218, 229, 234, 239
CviKI_1 RGCY 9 cut(s) 5, 56, 61, 82, 91, 218, 229, 234, 239
DdeI CTNAG 1 cut(s) 57
Ecl136II GAGCTC 1 cut(s) 61
Eco130I CCWWGG 1 cut(s) 134
Eco24I GRGCYC 1 cut(s) 63
Eco32I GATATC 1 cut(s) 34
Eco53kI GAGCTC 1 cut(s) 61
Eco57I CTGAAG 3 cut(s) 8, 128, 242
EcoICRI GAGCTC 1 cut(s) 61
EcoNI CCTNNNNNAGG 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 234
EcoRV GATATC 1 cut(s) 34
EcoT14I CCWWGG 1 cut(s) 134
EcoT38I GRGCYC 1 cut(s) 63
ErhI CCWWGG 1 cut(s) 134
FaeI CATG 1 cut(s) 121
FaiI YATR 3 cut(s) 119, 131, 150
FaqI GGGAC 1 cut(s) 285
FatI CATG 1 cut(s) 117
FokI GGATG 1 cut(s) 151
FriOI GRGCYC 1 cut(s) 63
GsuI CTGGAG 1 cut(s) 244
HaeIII GGCC 1 cut(s) 229
HgaI GACGC 1 cut(s) 109
Hin1I GRCGYC 1 cut(s) 101
Hin1II CATG 1 cut(s) 121
HphI GGTGA 2 cut(s) 38, 233
Hpy166II GTNNAC 1 cut(s) 14
Hpy188I TCNGA 2 cut(s) 27, 261
Hpy188III TCNNGA 1 cut(s) 85
Hpy8I GTNNAC 1 cut(s) 14
HpyAV CCTTC 1 cut(s) 266
HpyCH4III ACNGT 3 cut(s) 158, 179, 206
HpyCH4V TGCA 1 cut(s) 97
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 139
HpyF3I CTNAG 1 cut(s) 57
Hsp92I GRCGYC 1 cut(s) 101
Hsp92II CATG 1 cut(s) 121
LmnI GCTCC 1 cut(s) 258
LpnPI CCDG 7 cut(s) 2, 66, 208, 213, 221, 228, 248
LweI GCATC 2 cut(s) 129, 267
MaeIII GTNAC 2 cut(s) 121, 165
MboII GAAGA 1 cut(s) 274
MhlI GDGCHC 1 cut(s) 63
MnlI CCTC 4 cut(s) 63, 81, 219, 239
MspA1I CMGCKG 1 cut(s) 56
MspR9I CCNGG 1 cut(s) 236
MvaI CCWGG 1 cut(s) 236
MwoI GCNNNNNNNGC 2 cut(s) 88, 139
NlaIII CATG 1 cut(s) 121
NmeAIII GCCGAG 1 cut(s) 255
NmuCI GTSAC 1 cut(s) 165
Psp124BI GAGCTC 1 cut(s) 63
Psp6I CCWGG 1 cut(s) 234
PspGI CCWGG 1 cut(s) 234
PvuII CAGCTG 1 cut(s) 56
SacI GAGCTC 1 cut(s) 63
ScrFI CCNGG 1 cut(s) 236
SduI GDGCHC 1 cut(s) 63
SetI ASST 4 cut(s) 21, 58, 63, 84
SfaNI GCATC 2 cut(s) 129, 267
SmlI CTYRAG 1 cut(s) 83
SmoI CTYRAG 1 cut(s) 83
SstI GAGCTC 1 cut(s) 63
StyD4I CCNGG 1 cut(s) 234
StyI CCWWGG 1 cut(s) 134
TaaI ACNGT 3 cut(s) 158, 179, 206
TaqI TCGA 2 cut(s) 36, 63
TscAI CASTG 1 cut(s) 211
TseFI GTSAC 1 cut(s) 165
Tsp45I GTSAC 1 cut(s) 165
TspDTI ATGAA 1 cut(s) 106
TspRI CASTG 1 cut(s) 211
XagI CCTNNNNNAGG 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.