Rh4BG295900

ribosomal small subunit assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0020228
Physical Location & Seq
Reverse (-)
46757264 .. 46757935
672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG295900.1

Sequence Viewer

Length: 303 bp
ATGGTGCTTCAGAACGATATCGATTTGCTTCACCCACCGGCTGAGCTCGAGAAGAGGAAGCACAAGCTCAAGCGACTCGTGCAGACCCCGAACTCTTTCTTCATGTACGACTGTGTTCAGTCACTCCCAGACCGTTGTGGTGTGTGGGAATTGCCAGACTGTGCTGTGCCAGCCCACTGGTGGCCGTGCCAGACTCACCGAGGGATGCTCTTTCCGAAAGAAGGGGGATTGATTCATGCTGATATTGCTCTTGCAAGAGGCTTGATTTTGAGGATGGTTCTCATTTTTGTAATGGGTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

100

Amino Acids

11.52

Weight (kDa)

7.74

Isoelectric Point (pI)

40.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020228)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61111
fragaria_vesca FvH4_1g27030
pyrus_communis pycom16g10850
rosa_samantha Rh4AG290200 Rh4BG295900 Rh4CG305600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 182
AfaI GTAC 1 cut(s) 107
AfiI CCNNNNNNNGG 2 cut(s) 180, 221
AluBI AGCT 2 cut(s) 46, 67
AluI AGCT 2 cut(s) 46, 67
Alw21I GWGCWC 1 cut(s) 48
Ama87I CYCGRG 1 cut(s) 47
AoxI GGCC 1 cut(s) 182
Asp700I GAANNNNTTC 1 cut(s) 95
AsuHPI GGTGA 2 cut(s) 23, 188
AvaI CYCGRG 1 cut(s) 47
BanII GRGCYC 1 cut(s) 48
BauI CACGAG 1 cut(s) 77
Bbv12I GWGCWC 1 cut(s) 48
BccI CCATC 1 cut(s) 268
BceAI ACGGC 1 cut(s) 169
BfaI CTAG 1 cut(s) 301
BlpI GCTNAGC 1 cut(s) 42
BmeT110I CYCGRG 1 cut(s) 47
BmsI GCATC 1 cut(s) 195
BplI GAGNNNNNCTC 2 cut(s) 192, 224
Bpu1102I GCTNAGC 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 53
Bsa29I ATCGAT 1 cut(s) 21
BsaJI CCNNGG 1 cut(s) 199
Bsc4I CCNNNNNNNGG 2 cut(s) 180, 221
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 1 cut(s) 182
BseCI ATCGAT 1 cut(s) 21
BseDI CCNNGG 1 cut(s) 199
BseGI GGATG 2 cut(s) 210, 279
BseLI CCNNNNNNNGG 2 cut(s) 180, 221
BseMII CTCAG 1 cut(s) 33
BseNI ACTGG 1 cut(s) 182
BsgI GTGCAG 1 cut(s) 101
BshFI GGCC 1 cut(s) 184
BshVI ATCGAT 1 cut(s) 21
BsiHKAI GWGCWC 1 cut(s) 48
BsiHKCI CYCGRG 1 cut(s) 47
BsiSI CCGG 1 cut(s) 38
BslI CCNNNNNNNGG 2 cut(s) 180, 221
BsnI GGCC 1 cut(s) 184
BsoBI CYCGRG 1 cut(s) 47
Bsp1286I GDGCHC 1 cut(s) 48
Bsp1720I GCTNAGC 1 cut(s) 42
BspANI GGCC 1 cut(s) 184
BspCNI CTCAG 1 cut(s) 34
BspDI ATCGAT 1 cut(s) 21
BsrFI RCCGGY 1 cut(s) 37
BsrI ACTGG 1 cut(s) 182
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 1 cut(s) 199
BssSI CACGAG 1 cut(s) 77
Bst2BI CACGAG 1 cut(s) 77
Bst4CI ACNGT 3 cut(s) 113, 134, 161
Bst6I CTCTTC 1 cut(s) 47
BstC8I GCNNGC 1 cut(s) 171
BstDEI CTNAG 1 cut(s) 42
BstF5I GGATG 2 cut(s) 210, 279
BstMWI GCNNNNNNNGC 3 cut(s) 79, 170, 245
BstXI CCANNNNNNTGG 1 cut(s) 177
Bsu15I ATCGAT 1 cut(s) 21
BsuRI GGCC 1 cut(s) 184
BsuTUI ATCGAT 1 cut(s) 21
BtsCI GGATG 2 cut(s) 210, 279
BtsIMutI CAGTG 1 cut(s) 175
Cac8I GCNNGC 1 cut(s) 171
Cfr10I RCCGGY 1 cut(s) 37
ClaI ATCGAT 1 cut(s) 21
Csp6I GTAC 1 cut(s) 106
CviAII CATG 2 cut(s) 103, 236
CviJI RGCY 6 cut(s) 41, 46, 67, 173, 184, 261
CviKI_1 RGCY 6 cut(s) 41, 46, 67, 173, 184, 261
CviQI GTAC 1 cut(s) 106
DdeI CTNAG 1 cut(s) 42
EaeI YGGCCR 1 cut(s) 182
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
Ecl136II GAGCTC 1 cut(s) 46
Eco24I GRGCYC 1 cut(s) 48
Eco32I GATATC 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 46
Eco88I CYCGRG 1 cut(s) 47
EcoICRI GAGCTC 1 cut(s) 46
EcoRV GATATC 1 cut(s) 19
EcoT38I GRGCYC 1 cut(s) 48
FaeI CATG 2 cut(s) 106, 239
FaiI YATR 2 cut(s) 104, 237
FatI CATG 2 cut(s) 102, 235
FokI GGATG 2 cut(s) 217, 286
FriOI GRGCYC 1 cut(s) 48
FspBI CTAG 1 cut(s) 301
HaeIII GGCC 1 cut(s) 184
HapII CCGG 1 cut(s) 38
Hin1II CATG 2 cut(s) 106, 239
HinfI GANTC 3 cut(s) 75, 193, 232
HpaII CCGG 1 cut(s) 38
HphI GGTGA 2 cut(s) 23, 188
Hpy188I TCNGA 2 cut(s) 12, 216
Hpy188III TCNNGA 1 cut(s) 49
HpyAV CCTTC 1 cut(s) 215
HpyCH4III ACNGT 3 cut(s) 113, 134, 161
HpyCH4V TGCA 2 cut(s) 82, 254
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 170, 245
HpyF3I CTNAG 1 cut(s) 42
Hsp92II CATG 2 cut(s) 106, 239
LpnPI CCDG 6 cut(s) 51, 141, 163, 168, 183, 203
LweI GCATC 1 cut(s) 195
MaeI CTAG 1 cut(s) 301
MaeIII GTNAC 1 cut(s) 120
MboII GAAGA 2 cut(s) 64, 91
MhlI GDGCHC 1 cut(s) 48
MluCI AATT 1 cut(s) 149
MlyI GAGTC 2 cut(s) 69, 187
MnlI CCTC 4 cut(s) 48, 194, 251, 264
MroXI GAANNNNTTC 1 cut(s) 95
MspI CCGG 1 cut(s) 38
MwoI GCNNNNNNNGC 3 cut(s) 79, 170, 245
NlaIII CATG 2 cut(s) 106, 239
NmuCI GTSAC 1 cut(s) 120
PaeR7I CTCGAG 1 cut(s) 47
PdmI GAANNNNTTC 1 cut(s) 95
PfeI GAWTC 1 cut(s) 232
PleI GAGTC 2 cut(s) 69, 187
PpsI GAGTC 2 cut(s) 69, 187
Psp124BI GAGCTC 1 cut(s) 48
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
SacI GAGCTC 1 cut(s) 48
SchI GAGTC 2 cut(s) 69, 187
SduI GDGCHC 1 cut(s) 48
SetI ASST 2 cut(s) 48, 69
SfaNI GCATC 1 cut(s) 195
Sfr274I CTCGAG 1 cut(s) 47
SlaI CTCGAG 1 cut(s) 47
SmlI CTYRAG 2 cut(s) 47, 68
SmoI CTYRAG 2 cut(s) 47, 68
Sse9I AATT 1 cut(s) 149
SspMI CTAG 1 cut(s) 301
SstI GAGCTC 1 cut(s) 48
TaaI ACNGT 3 cut(s) 113, 134, 161
TaqI TCGA 2 cut(s) 21, 48
TasI AATT 1 cut(s) 149
TfiI GAWTC 1 cut(s) 232
TscAI CASTG 1 cut(s) 182
TseFI GTSAC 1 cut(s) 120
Tsp45I GTSAC 1 cut(s) 120
TspDTI ATGAA 2 cut(s) 91, 224
TspRI CASTG 1 cut(s) 182
XcmI CCANNNNNNNNNTGG 1 cut(s) 177
XhoI CTCGAG 1 cut(s) 47
XmnI GAANNNNTTC 1 cut(s) 95
XspI CTAG 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.