pycom16g11020

Light-regulated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014393
Physical Location & Seq
Reverse (-)
7629539 .. 7630629
1091 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 747 bp
ATGGAGCTTTTTGTCACCCTCATGTATAATAAACATGATGTGCGAAAAGAGAAAAAAATAATATTGCATAAATTAATTCCCAAAAAAGGGAAGATATGGAGAAGAAATGAGGAAGAAAAGTCCAATTTCATGGGTATTTTTCAACGTTTAATGGTGCACCAAGAAGTTATGACCTTATCTTTTCTCAAAAAATCTTTGCCACCTTATCCAATATCCCATCCAAGTCATGTCCCAAAGCTGATGCTATATAGTTTGGCTGGAACTTCGCCCCTCTTTAGACTCAGCAAATTAAACCCAACTTCTAAATTACAAGCCATCAAAATGCAGGCCACTCTACGTCTCTCACCAATTTCATCAGTATCACCTCTTGCTTTTGTAACCACCAAGTCCCCGGCCTTGCCATGCGGCTCCCTTCCGAGGCCACTACAGCCCTCAAGGTGCCCTCCCCTGAGGGCAACCAATGTGACAGGCGGCGATCCTGCATCTGTCGATTACAGCTCCATCACTTCTGTTTTCCCGGCAGAGGCTTGCGAAACAGTCGGAGGAGAAGCTTGTGATGCCGAAATGTATCCCGAAGTGAAGCTCAAACCGGAAGAGGGCAGGAGCAACACAGCCAGGACTGTTTCTGAGCAGCTGGATAGAGATTATCTAGAGTATGACAGTCCCAAGACGGTCTTTCCTGGGGAGGCTTGTGATGATCTGGGAGGAGAATTCTGTGAGCCAGAGTATCAGAGAGGGGTAAACTAG

Protein Analysis

249

Amino Acids

27.7

Weight (kDa)

8.65

Isoelectric Point (pI)

56.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lir1 PF07207 141 - 240 2.6e-43 Light regulated protein Lir1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26740
fragaria_vesca FvH4_4g22790 FvH4_4g22790 FvH4_4g22790
malus_domestica MD16G1129900.v1.1
prunus_persica Prupe.1G216700_v2.0.a1
pyrus_communis pycom16g11020
rosa_chinensis RchiOBHm_Chr4g0429011
rosa_laevigata RLG00000007104
rosa_multiflora Rmu_sc0008629.1_g000017
rosa_rugosa Rorug04G0232000
rosa_samantha Rh4AG288000 Rh4BG294200 Rh4CG309700 Rh4DG291100
rosa_wichuraiana Rw0G014070 Rw4G024960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 438
AciI CCGC 2 cut(s) 405, 471
AclI AACGTT 1 cut(s) 145
AclWI GGATC 1 cut(s) 470
AcsI RAATTY 1 cut(s) 710
AfiI CCNNNNNNNGG 5 cut(s) 86, 87, 417, 523, 596
AgsI TTSAA 1 cut(s) 143
AjnI CCWGG 2 cut(s) 614, 679
AluBI AGCT 6 cut(s) 7, 238, 498, 551, 583, 634
AluI AGCT 6 cut(s) 7, 238, 498, 551, 583, 634
Alw21I GWGCWC 1 cut(s) 159
Alw26I GTCTC 1 cut(s) 344
Alw44I GTGCAC 1 cut(s) 155
AlwI GGATC 1 cut(s) 470
AoxI GGCC 3 cut(s) 327, 393, 419
ApaLI GTGCAC 1 cut(s) 155
ApeKI GCWGC 1 cut(s) 631
ApoI RAATTY 1 cut(s) 710
AseI ATTAAT 1 cut(s) 74
AsuC2I CCSGG 2 cut(s) 392, 518
AsuHPI GGTGA 3 cut(s) 7, 336, 354
AxyI CCTNAGG 1 cut(s) 449
BaeGI GKGCMC 2 cut(s) 159, 443
BanI GGYRCC 1 cut(s) 438
Bbv12I GWGCWC 1 cut(s) 159
BbvI GCAGC 1 cut(s) 643
BccI CCATC 3 cut(s) 225, 323, 509
BciT130I CCWGG 2 cut(s) 616, 681
BciVI GTATCC 1 cut(s) 579
BcnI CCSGG 2 cut(s) 392, 518
BcoDI GTCTC 1 cut(s) 344
BfaI CTAG 2 cut(s) 650, 745
BfmI CTRYAG 1 cut(s) 425
BfuI GTATCC 1 cut(s) 579
BisI GCNGC 3 cut(s) 406, 472, 632
BlsI GCNGC 3 cut(s) 407, 473, 633
Bme1390I CCNGG 4 cut(s) 392, 518, 616, 681
BmiI GGNNCC 2 cut(s) 409, 440
BmrFI CCNGG 4 cut(s) 392, 518, 616, 681
BmsI GCATC 3 cut(s) 231, 491, 547
BpuEI CTTGAG 1 cut(s) 418
BpuMI CCSGG 2 cut(s) 392, 518
BsaJI CCNNGG 3 cut(s) 390, 416, 680
BsaWI WCCGGW 1 cut(s) 589
Bsc4I CCNNNNNNNGG 5 cut(s) 86, 87, 417, 523, 596
Bse21I CCTNAGG 1 cut(s) 449
BseBI CCWGG 2 cut(s) 616, 681
BseDI CCNNGG 3 cut(s) 390, 416, 680
BseGI GGATG 1 cut(s) 217
BseLI CCNNNNNNNGG 5 cut(s) 86, 87, 417, 523, 596
BseMII CTCAG 3 cut(s) 295, 440, 618
BseRI GAGGAG 2 cut(s) 558, 720
BseSI GKGCMC 2 cut(s) 159, 443
BseXI GCAGC 1 cut(s) 643
BshFI GGCC 3 cut(s) 329, 395, 421
BshNI GGYRCC 1 cut(s) 438
BsiHKAI GWGCWC 1 cut(s) 159
BsiSI CCGG 3 cut(s) 392, 518, 590
BslFI GGGAC 3 cut(s) 215, 373, 648
BslI CCNNNNNNNGG 5 cut(s) 86, 87, 417, 523, 596
BsmAI GTCTC 1 cut(s) 344
BsmBI CGTCTC 1 cut(s) 344
BsmFI GGGAC 3 cut(s) 215, 373, 648
BsnI GGCC 3 cut(s) 329, 395, 421
Bsp1286I GDGCHC 2 cut(s) 159, 443
Bsp143I GATC 2 cut(s) 475, 697
BspACI CCGC 2 cut(s) 405, 471
BspANI GGCC 3 cut(s) 329, 395, 421
BspCNI CTCAG 3 cut(s) 294, 441, 619
BspLI GGNNCC 2 cut(s) 409, 440
BspPI GGATC 1 cut(s) 470
BspT107I GGYRCC 1 cut(s) 438
BssECI CCNNGG 3 cut(s) 390, 416, 680
BssMI GATC 2 cut(s) 475, 697
Bst2UI CCWGG 2 cut(s) 616, 681
Bst4CI ACNGT 4 cut(s) 538, 622, 662, 673
Bst6I CTCTTC 1 cut(s) 588
BstC8I GCNNGC 2 cut(s) 327, 529
BstDEI CTNAG 3 cut(s) 281, 449, 627
BstF5I GGATG 1 cut(s) 217
BstKTI GATC 2 cut(s) 478, 700
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 2 cut(s) 475, 697
BstMWI GCNNNNNNNGC 2 cut(s) 427, 557
BstNI CCWGG 2 cut(s) 616, 681
BstSCI CCNGG 4 cut(s) 390, 516, 614, 679
BstSFI CTRYAG 1 cut(s) 425
BstSLI GKGCMC 2 cut(s) 159, 443
BstV1I GCAGC 1 cut(s) 643
BstXI CCANNNNNNTGG 1 cut(s) 130
Bsu36I CCTNAGG 1 cut(s) 449
BsuI GTATCC 1 cut(s) 579
BsuRI GGCC 3 cut(s) 329, 395, 421
BtsCI GGATG 1 cut(s) 217
Cac8I GCNNGC 2 cut(s) 327, 529
CviAII CATG 5 cut(s) 22, 35, 130, 227, 402
DdeI CTNAG 3 cut(s) 281, 449, 627
DpnI GATC 2 cut(s) 477, 699
DpnII GATC 2 cut(s) 475, 697
Eam1104I CTCTTC 1 cut(s) 588
EarI CTCTTC 1 cut(s) 588
Eco81I CCTNAGG 1 cut(s) 449
EcoRI GAATTC 1 cut(s) 710
EcoRII CCWGG 2 cut(s) 614, 679
Esp3I CGTCTC 1 cut(s) 344
FaeI CATG 5 cut(s) 25, 38, 133, 230, 405
FalI AAGNNNNNCTT 2 cut(s) 659, 691
FaqI GGGAC 3 cut(s) 215, 373, 648
FatI CATG 5 cut(s) 21, 34, 129, 226, 401
Fnu4HI GCNGC 3 cut(s) 406, 472, 632
FokI GGATG 1 cut(s) 204
Fsp4HI GCNGC 3 cut(s) 406, 472, 632
FspBI CTAG 2 cut(s) 650, 745
GluI GCNGC 3 cut(s) 406, 472, 632
HaeIII GGCC 3 cut(s) 329, 395, 421
HapII CCGG 3 cut(s) 392, 518, 590
Hin1II CATG 5 cut(s) 25, 38, 133, 230, 405
HindIII AAGCTT 1 cut(s) 549
HinfI GANTC 1 cut(s) 279
HpaII CCGG 3 cut(s) 392, 518, 590
HphI GGTGA 3 cut(s) 7, 336, 354
Hpy166II GTNNAC 2 cut(s) 157, 742
Hpy188I TCNGA 4 cut(s) 417, 542, 628, 732
Hpy188III TCNNGA 2 cut(s) 572, 650
Hpy8I GTNNAC 2 cut(s) 157, 742
HpyAV CCTTC 1 cut(s) 422
HpyCH4III ACNGT 4 cut(s) 538, 622, 662, 673
HpyCH4IV ACGT 2 cut(s) 145, 337
HpyCH4V TGCA 4 cut(s) 67, 157, 325, 482
HpyF10VI GCNNNNNNNGC 2 cut(s) 427, 557
HpyF3I CTNAG 3 cut(s) 281, 449, 627
HpySE526I ACGT 2 cut(s) 145, 337
Hsp92II CATG 5 cut(s) 25, 38, 133, 230, 405
Kzo9I GATC 2 cut(s) 475, 697
LmnI GCTCC 4 cut(s) 4, 413, 503, 603
Lsp1109I GCAGC 1 cut(s) 643
LweI GCATC 3 cut(s) 231, 491, 547
MaeI CTAG 2 cut(s) 650, 745
MaeII ACGT 2 cut(s) 145, 337
MaeIII GTNAC 3 cut(s) 13, 376, 463
MalI GATC 2 cut(s) 477, 699
MboI GATC 2 cut(s) 475, 697
MboII GAAGA 4 cut(s) 103, 114, 125, 605
MhlI GDGCHC 2 cut(s) 159, 443
MluCI AATT 7 cut(s) 71, 75, 124, 287, 305, 348, 710
MlyI GAGTC 1 cut(s) 273
MmeI TCCRAC 1 cut(s) 520
MseI TTAA 3 cut(s) 74, 149, 290
MslI CAYNNNNRTG 2 cut(s) 20, 320
MspA1I CMGCKG 1 cut(s) 634
MspI CCGG 3 cut(s) 392, 518, 590
MspR9I CCNGG 4 cut(s) 392, 518, 616, 681
MvaI CCWGG 2 cut(s) 616, 681
MwoI GCNNNNNNNGC 2 cut(s) 427, 557
NciI CCSGG 2 cut(s) 392, 518
NdeII GATC 2 cut(s) 475, 697
NlaIII CATG 5 cut(s) 25, 38, 133, 230, 405
NlaIV GGNNCC 2 cut(s) 409, 440
NmuCI GTSAC 2 cut(s) 13, 463
PkrI GCNGC 3 cut(s) 407, 473, 633
PleI GAGTC 1 cut(s) 273
PpsI GAGTC 1 cut(s) 273
PshBI ATTAAT 1 cut(s) 74
Psp1406I AACGTT 1 cut(s) 145
Psp6I CCWGG 2 cut(s) 614, 679
PspGI CCWGG 2 cut(s) 614, 679
PspN4I GGNNCC 2 cut(s) 409, 440
PvuII CAGCTG 1 cut(s) 634
RseI CAYNNNNRTG 2 cut(s) 20, 320
SaqAI TTAA 3 cut(s) 74, 149, 290
SatI GCNGC 3 cut(s) 406, 472, 632
Sau3AI GATC 2 cut(s) 475, 697
SchI GAGTC 1 cut(s) 273
ScrFI CCNGG 4 cut(s) 392, 518, 616, 681
SduI GDGCHC 2 cut(s) 159, 443
SfaNI GCATC 3 cut(s) 231, 491, 547
SfcI CTRYAG 1 cut(s) 425
SmiMI CAYNNNNRTG 2 cut(s) 20, 320
SmlI CTYRAG 1 cut(s) 433
SmoI CTYRAG 1 cut(s) 433
Sse9I AATT 7 cut(s) 71, 75, 124, 287, 305, 348, 710
SsiI CCGC 2 cut(s) 405, 471
SspI AATATT 1 cut(s) 63
SspMI CTAG 2 cut(s) 650, 745
StyD4I CCNGG 4 cut(s) 390, 516, 614, 679
TaaI ACNGT 4 cut(s) 538, 622, 662, 673
TaiI ACGT 2 cut(s) 148, 340
TaqI TCGA 1 cut(s) 489
TasI AATT 7 cut(s) 71, 75, 124, 287, 305, 348, 710
TauI GCSGC 2 cut(s) 408, 474
Tru1I TTAA 3 cut(s) 74, 149, 290
Tru9I TTAA 3 cut(s) 74, 149, 290
TseFI GTSAC 2 cut(s) 13, 463
TseI GCWGC 1 cut(s) 631
Tsp45I GTSAC 2 cut(s) 13, 463
TspDTI ATGAA 2 cut(s) 118, 342
VneI GTGCAC 1 cut(s) 155
VspI ATTAAT 1 cut(s) 74
XapI RAATTY 1 cut(s) 710
XbaI TCTAGA 1 cut(s) 649
XspI CTAG 2 cut(s) 650, 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.