pycom16g13240

Dihydrolipoyl dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0022567
Physical Location & Seq
Forward (+)
9420603 .. 9424153
3551 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 888 bp
ATGAATAGGATTTTCACCTGCGGTCGTCACACTAGTTTACACCCAGTCGGCTCCGTGCGCATTTTAGCAAACATGCCAACTAACCAGAAAGAGGTAGTTCATCCGCCGCACTTTGATATCTCCGTCATTCCTTCTACCTCTTTACTACTCAACGGACCATTCGAGGCCTCCTCCCTTTCCTTAATAACACACTCCCTCTCTCATTTCCTTCATTTTCTCTCTCTAGATCCTCTCTCTCCTCACATTCCCAAAATGCAGGCCTCCCTCTCTCTGCCCTCCCTCTCCCACGCCGTCCCCAGATCAAACTACGTCGTTCAATCCGCCCTCCCTCTCCCGCCGTCCAAGCCTATCAATCTCCGATTCTGCGGCCTCCGCCGCGAGGCCTTGGGATTCTCGCCTCTCGCCCACTCCGACTCGCACCGCGTCCGTCTCTCAGCTCCTTCCCGCTCTGCAACAATCTCGGCTTCGTCCTCCTCCGGCAATGGCAGCCCTCCCAAGTCGTTCGACTACGACTTGCTCATCATCGGCGCCGGCGTCGGTGGCCACGGCGCTGCTCTCCACGCCGTTGAGAAGGGTTTAAAAACTGCCATCGTGGAGGGAGATGTGGTGGGTGGAACATGTGTTAACAGGGGATGTGTTCCATCTAAAGCTCTTCTGGCAGTTAGTGGTCGGATGCGTGAGCTTCAGAATGAACATCACCTTAAGGCTTTAGGTTTGCAGGTTTCAGCAGCTGGATATGACAGACAGGGAGTAGCTGATCATGATAATAATCTTGCAACAAAAATTCGTAATAGTTTGACCAATTCTATGAATTCTCTTGGAGTGGACATTCTGACAGGTGTTGGTACAATTCTGGGCCCTCAAAAGGTGCAAATTGGATCGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004148 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006082 GO:0006084 GO:0006085 GO:0006086 GO:0006090 GO:0006139 GO:0006163 GO:0006164 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0009987 GO:0016491 GO:0016651 GO:0016667 GO:0016668 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019752 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032787 GO:0033865 GO:0033866 GO:0033875 GO:0034030 GO:0034032 GO:0034033 GO:0034641 GO:0034654 GO:0035383 GO:0035384 GO:0042221 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046390 GO:0046483 GO:0046685 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0055114 GO:0070013 GO:0071616 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00020 ko00260 ko00280 ko00620 ko00630 ko00640 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00260 map00280 map00620 map00630 map00640 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
Pyr_redox_2 Pyr_redox_dim

Protein Analysis

296

Amino Acids

31.12

Weight (kDa)

9.61

Isoelectric Point (pI)

37.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_2 PF07992 170 - 290 1.3e-13 Pyridine nucleotide-disulphide oxidoreductase
FAD_oxidored PF12831 171 - 234 2.9e-06 FAD dependent oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022567)

Species Orthologous Gene IDs
pyrus_communis pycom16g13240
rosa_multiflora Rmu_co8199018.1_g000001 Rmu_sc0003885.1_g000007 Rmu_sc0003942.1_g000027

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 26
Acc16I TGCGCA 1 cut(s) 59
Acc36I ACCTGC 2 cut(s) 26, 709
AccB1I GGYRCC 1 cut(s) 527
AccBSI CCGCTC 1 cut(s) 447
AccII CGCG 2 cut(s) 378, 423
AclWI GGATC 2 cut(s) 221, 886
AcoI YGGCCR 1 cut(s) 541
AcsI RAATTY 2 cut(s) 783, 811
AcuI CTGAAG 1 cut(s) 668
AcyI GRCGYC 2 cut(s) 528, 534
AfaI GTAC 1 cut(s) 847
AfiI CCNNNNNNNGG 3 cut(s) 91, 379, 865
AflII CTTAAG 1 cut(s) 701
AflIII ACRYGT 1 cut(s) 617
AgsI TTSAA 1 cut(s) 317
AhlI ACTAGT 1 cut(s) 32
AluBI AGCT 5 cut(s) 437, 650, 682, 731, 755
AluI AGCT 5 cut(s) 437, 650, 682, 731, 755
Alw26I GTCTC 1 cut(s) 434
AlwI GGATC 2 cut(s) 221, 886
AlwNI CAGNNNCTG 1 cut(s) 731
AoxI GGCC 6 cut(s) 165, 258, 367, 381, 541, 856
ApaI GGGCCC 1 cut(s) 860
ApeKI GCWGC 3 cut(s) 486, 551, 728
ApoI RAATTY 2 cut(s) 783, 811
AspLEI GCGC 3 cut(s) 60, 530, 551
AspS9I GGNCC 3 cut(s) 155, 856, 857
AsuHPI GGTGA 2 cut(s) 7, 689
AvaII GGWCC 1 cut(s) 155
BaeGI GKGCMC 1 cut(s) 860
BalI TGGCCA 1 cut(s) 543
BanI GGYRCC 1 cut(s) 527
BanII GRGCYC 1 cut(s) 860
BbvI GCAGC 3 cut(s) 498, 538, 740
BccI CCATC 2 cut(s) 596, 649
BceAI ACGGC 4 cut(s) 275, 322, 548, 562
BcgI CGANNNNNNTGC 2 cut(s) 441, 475
BclI TGATCA 1 cut(s) 757
BcoDI GTCTC 1 cut(s) 434
BcuI ACTAGT 1 cut(s) 32
BfaI CTAG 2 cut(s) 33, 224
BfoI RGCGCY 2 cut(s) 531, 552
BfrI CTTAAG 1 cut(s) 701
BfuAI ACCTGC 2 cut(s) 26, 709
BisI GCNGC 6 cut(s) 107, 367, 376, 487, 552, 729
BlsI GCNGC 6 cut(s) 108, 368, 377, 488, 553, 730
Bme18I GGWCC 1 cut(s) 155
BmgT120I GGNCC 3 cut(s) 155, 856, 857
BmiI GGNNCC 3 cut(s) 52, 529, 858
BmrI ACTGGG 1 cut(s) 38
BmsI GCATC 1 cut(s) 663
BmuI ACTGGG 1 cut(s) 38
BplI GAGNNNNNCTC 2 cut(s) 155, 187
Bsa29I ATCGAT 1 cut(s) 881
BsaBI GATNNNNATC 1 cut(s) 768
BsaHI GRCGYC 2 cut(s) 528, 534
BsaJI CCNNGG 2 cut(s) 384, 544
BsaXI ACNNNNNCTCC 2 cut(s) 591, 621
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 379, 865
Bse118I RCCGGY 1 cut(s) 530
Bse1I ACTGG 1 cut(s) 44
Bse3DI GCAATG 1 cut(s) 487
Bse8I GATNNNNATC 1 cut(s) 768
BseCI ATCGAT 1 cut(s) 881
BseDI CCNNGG 2 cut(s) 384, 544
BseGI GGATG 3 cut(s) 100, 638, 678
BseJI GATNNNNATC 1 cut(s) 768
BseLI CCNNNNNNNGG 3 cut(s) 91, 379, 865
BseMI GCAATG 1 cut(s) 487
BseMII CTCAG 1 cut(s) 447
BseNI ACTGG 1 cut(s) 44
BseRI GAGGAG 3 cut(s) 160, 228, 463
BseSI GKGCMC 1 cut(s) 860
BseXI GCAGC 3 cut(s) 498, 538, 740
Bsh1236I CGCG 2 cut(s) 378, 423
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 6 cut(s) 167, 260, 369, 383, 543, 858
BshNI GGYRCC 1 cut(s) 527
BshVI ATCGAT 1 cut(s) 881
BsiEI CGRYCG 1 cut(s) 25
BsiSI CCGG 2 cut(s) 477, 531
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 3 cut(s) 91, 379, 865
BsmAI GTCTC 1 cut(s) 434
BsmBI CGTCTC 1 cut(s) 434
BsmFI GGGAC 1 cut(s) 278
BsnI GGCC 6 cut(s) 167, 260, 369, 383, 543, 858
Bsp120I GGGCCC 1 cut(s) 856
Bsp1286I GDGCHC 1 cut(s) 860
Bsp143I GATC 5 cut(s) 226, 299, 757, 878, 882
BspANI GGCC 6 cut(s) 167, 260, 369, 383, 543, 858
BspCNI CTCAG 1 cut(s) 446
BspDI ATCGAT 1 cut(s) 881
BspFNI CGCG 2 cut(s) 378, 423
BspHI TCATGA 1 cut(s) 760
BspLI GGNNCC 3 cut(s) 52, 529, 858
BspMI ACCTGC 2 cut(s) 26, 709
BspPI GGATC 2 cut(s) 221, 886
BspQI GCTCTTC 1 cut(s) 657
BspT107I GGYRCC 1 cut(s) 527
BspTI CTTAAG 1 cut(s) 701
BsrBI CCGCTC 1 cut(s) 447
BsrDI GCAATG 1 cut(s) 487
BsrFI RCCGGY 1 cut(s) 530
BsrI ACTGG 1 cut(s) 44
BssAI RCCGGY 1 cut(s) 530
BssECI CCNNGG 2 cut(s) 384, 544
BssMI GATC 5 cut(s) 226, 299, 757, 878, 882
BssNI GRCGYC 2 cut(s) 528, 534
BssT1I CCWWGG 1 cut(s) 384
Bst6I CTCTTC 1 cut(s) 657
BstACI GRCGYC 2 cut(s) 528, 534
BstAFI CTTAAG 1 cut(s) 701
BstC8I GCNNGC 2 cut(s) 258, 532
BstDEI CTNAG 1 cut(s) 433
BstDSI CCRYGG 1 cut(s) 544
BstF5I GGATG 3 cut(s) 100, 638, 678
BstFNI CGCG 2 cut(s) 378, 423
BstH2I RGCGCY 2 cut(s) 531, 552
BstHHI GCGC 3 cut(s) 60, 530, 551
BstKTI GATC 5 cut(s) 229, 302, 760, 881, 885
BstMAI GTCTC 1 cut(s) 434
BstMBI GATC 5 cut(s) 226, 299, 757, 878, 882
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 8 cut(s) 57, 343, 372, 375, 486, 540, 560, 656
BstNSI RCATGY 2 cut(s) 76, 621
BstSLI GKGCMC 1 cut(s) 860
BstUI CGCG 2 cut(s) 378, 423
BstV1I GCAGC 3 cut(s) 498, 538, 740
BstX2I RGATCY 1 cut(s) 226
BstYI RGATCY 1 cut(s) 226
Bsu15I ATCGAT 1 cut(s) 881
BsuRI GGCC 6 cut(s) 167, 260, 369, 383, 543, 858
BsuTUI ATCGAT 1 cut(s) 881
BtgI CCRYGG 1 cut(s) 544
BtsCI GGATG 3 cut(s) 100, 638, 678
BveI ACCTGC 2 cut(s) 26, 709
Cac8I GCNNGC 2 cut(s) 258, 532
CaiI CAGNNNCTG 1 cut(s) 731
CciI TCATGA 1 cut(s) 760
CfoI GCGC 3 cut(s) 60, 530, 551
Cfr10I RCCGGY 1 cut(s) 530
Cfr13I GGNCC 3 cut(s) 155, 856, 857
ClaI ATCGAT 1 cut(s) 881
CseI GACGC 2 cut(s) 412, 523
Csp6I GTAC 1 cut(s) 846
CviAII CATG 3 cut(s) 73, 618, 761
CviQI GTAC 1 cut(s) 846
DdeI CTNAG 1 cut(s) 433
DinI GGCGCC 1 cut(s) 529
DpnI GATC 5 cut(s) 228, 301, 759, 880, 884
DpnII GATC 5 cut(s) 226, 299, 757, 878, 882
DraI TTTAAA 1 cut(s) 579
EaeI YGGCCR 1 cut(s) 541
Eam1104I CTCTTC 1 cut(s) 657
EarI CTCTTC 1 cut(s) 657
EciI GGCGGA 3 cut(s) 93, 310, 362
Eco130I CCWWGG 1 cut(s) 384
Eco147I AGGCCT 3 cut(s) 167, 260, 383
Eco24I GRGCYC 1 cut(s) 860
Eco32I GATATC 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 155
Eco57I CTGAAG 1 cut(s) 668
EcoO109I RGGNCCY 1 cut(s) 857
EcoRI GAATTC 1 cut(s) 811
EcoRV GATATC 1 cut(s) 118
EcoT14I CCWWGG 1 cut(s) 384
EcoT38I GRGCYC 1 cut(s) 860
EgeI GGCGCC 1 cut(s) 529
EheI GGCGCC 1 cut(s) 529
ErhI CCWWGG 1 cut(s) 384
Esp3I CGTCTC 1 cut(s) 434
FaeI CATG 3 cut(s) 76, 621, 764
FaiI YATR 5 cut(s) 74, 619, 738, 762, 809
FaqI GGGAC 1 cut(s) 278
FatI CATG 3 cut(s) 72, 617, 760
FauI CCCGC 2 cut(s) 342, 452
FbaI TGATCA 1 cut(s) 757
Fnu4HI GCNGC 6 cut(s) 107, 367, 376, 487, 552, 729
FokI GGATG 3 cut(s) 87, 645, 685
FriOI GRGCYC 1 cut(s) 860
Fsp4HI GCNGC 6 cut(s) 107, 367, 376, 487, 552, 729
FspAI RTGCGCAY 1 cut(s) 59
FspBI CTAG 2 cut(s) 33, 224
FspI TGCGCA 1 cut(s) 59
GlaI GCGC 3 cut(s) 59, 529, 550
GluI GCNGC 6 cut(s) 107, 367, 376, 487, 552, 729
HaeII RGCGCY 2 cut(s) 531, 552
HaeIII GGCC 6 cut(s) 167, 260, 369, 383, 543, 858
HapII CCGG 2 cut(s) 477, 531
HgaI GACGC 2 cut(s) 412, 523
HhaI GCGC 3 cut(s) 60, 530, 551
Hin1I GRCGYC 2 cut(s) 528, 534
Hin1II CATG 3 cut(s) 76, 621, 764
Hin6I GCGC 3 cut(s) 58, 528, 549
HinP1I GCGC 3 cut(s) 58, 528, 549
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HinfI GANTC 3 cut(s) 360, 390, 413
HpaI GTTAAC 1 cut(s) 625
HpaII CCGG 2 cut(s) 477, 531
HphI GGTGA 2 cut(s) 7, 689
Hpy166II GTNNAC 3 cut(s) 38, 625, 826
Hpy188I TCNGA 6 cut(s) 359, 412, 672, 687, 834, 887
Hpy188III TCNNGA 2 cut(s) 224, 761
Hpy8I GTNNAC 3 cut(s) 38, 625, 826
Hpy99I CGWCG 2 cut(s) 314, 539
HpyAV CCTTC 4 cut(s) 141, 218, 450, 565
HpyCH4IV ACGT 1 cut(s) 309
HpyCH4V TGCA 5 cut(s) 256, 452, 718, 776, 871
HpyF10VI GCNNNNNNNGC 8 cut(s) 57, 343, 372, 375, 486, 540, 560, 656
HpyF3I CTNAG 1 cut(s) 433
HpySE526I ACGT 1 cut(s) 309
Hsp92I GRCGYC 2 cut(s) 528, 534
Hsp92II CATG 3 cut(s) 76, 621, 764
HspAI GCGC 3 cut(s) 58, 528, 549
KasI GGCGCC 1 cut(s) 527
KroI GCCGGC 1 cut(s) 530
KroNI GCCGGC 1 cut(s) 532
Ksp22I TGATCA 1 cut(s) 757
KspAI GTTAAC 1 cut(s) 625
Kzo9I GATC 5 cut(s) 226, 299, 757, 878, 882
LguI GCTCTTC 1 cut(s) 657
LmnI GCTCC 2 cut(s) 56, 442
Lsp1109I GCAGC 3 cut(s) 498, 538, 740
LweI GCATC 1 cut(s) 663
MaeI CTAG 2 cut(s) 33, 224
MaeII ACGT 1 cut(s) 309
MaeIII GTNAC 1 cut(s) 26
MalI GATC 5 cut(s) 228, 301, 759, 880, 884
MbiI CCGCTC 1 cut(s) 447
MboI GATC 5 cut(s) 226, 299, 757, 878, 882
MboII GAAGA 1 cut(s) 644
MflI RGATCY 1 cut(s) 226
MhlI GDGCHC 1 cut(s) 860
MlsI TGGCCA 1 cut(s) 543
MluCI AATT 5 cut(s) 783, 802, 811, 849, 873
MluNI TGGCCA 1 cut(s) 543
Mly113I GGCGCC 1 cut(s) 528
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 2 cut(s) 435, 650
Mox20I TGGCCA 1 cut(s) 543
MreI CGCCGGCG 1 cut(s) 530
MroNI GCCGGC 1 cut(s) 530
MscI TGGCCA 1 cut(s) 543
MseI TTAA 4 cut(s) 182, 578, 624, 702
Msp20I TGGCCA 1 cut(s) 543
MspA1I CMGCKG 1 cut(s) 731
MspCI CTTAAG 1 cut(s) 701
MspI CCGG 2 cut(s) 477, 531
MvnI CGCG 2 cut(s) 378, 423
MwoI GCNNNNNNNGC 8 cut(s) 57, 343, 372, 375, 486, 540, 560, 656
NaeI GCCGGC 1 cut(s) 532
NarI GGCGCC 1 cut(s) 528
NdeII GATC 5 cut(s) 226, 299, 757, 878, 882
NgoMIV GCCGGC 1 cut(s) 530
NlaIII CATG 3 cut(s) 76, 621, 764
NlaIV GGNNCC 3 cut(s) 52, 529, 858
NmeAIII GCCGAG 1 cut(s) 440
NmuCI GTSAC 1 cut(s) 26
NsbI TGCGCA 1 cut(s) 59
NspI RCATGY 2 cut(s) 76, 621
PagI TCATGA 1 cut(s) 760
PaqCI CACCTGC 1 cut(s) 26
PceI AGGCCT 3 cut(s) 167, 260, 383
PciI ACATGT 1 cut(s) 617
PciSI GCTCTTC 1 cut(s) 657
PcsI WCGNNNNNNNCGW 3 cut(s) 159, 408, 531
PdiI GCCGGC 1 cut(s) 532
PfeI GAWTC 2 cut(s) 360, 390
PkrI GCNGC 6 cut(s) 108, 368, 377, 488, 553, 730
PleI GAGTC 1 cut(s) 407
PluTI GGCGCC 1 cut(s) 531
PpsI GAGTC 1 cut(s) 407
PscI ACATGT 1 cut(s) 617
PspN4I GGNNCC 3 cut(s) 52, 529, 858
PspOMI GGGCCC 1 cut(s) 856
PspPI GGNCC 3 cut(s) 155, 856, 857
PstNI CAGNNNCTG 1 cut(s) 731
PsuI RGATCY 1 cut(s) 226
PvuII CAGCTG 1 cut(s) 731
RsaI GTAC 1 cut(s) 847
RsaNI GTAC 1 cut(s) 846
SapI GCTCTTC 1 cut(s) 657
SaqAI TTAA 4 cut(s) 182, 578, 624, 702
SatI GCNGC 6 cut(s) 107, 367, 376, 487, 552, 729
Sau3AI GATC 5 cut(s) 226, 299, 757, 878, 882
Sau96I GGNCC 3 cut(s) 155, 856, 857
SchI GAGTC 1 cut(s) 407
SduI GDGCHC 1 cut(s) 860
SfaNI GCATC 1 cut(s) 663
SfoI GGCGCC 1 cut(s) 529
SgrAI CRCCGGYG 1 cut(s) 530
SinI GGWCC 1 cut(s) 155
SmlI CTYRAG 1 cut(s) 701
SmoI CTYRAG 1 cut(s) 701
SpeI ACTAGT 1 cut(s) 32
Sse9I AATT 5 cut(s) 783, 802, 811, 849, 873
SseBI AGGCCT 3 cut(s) 167, 260, 383
SspDI GGCGCC 1 cut(s) 527
SspMI CTAG 2 cut(s) 33, 224
StuI AGGCCT 3 cut(s) 167, 260, 383
StyI CCWWGG 1 cut(s) 384
TaiI ACGT 1 cut(s) 312
TaqI TCGA 3 cut(s) 162, 504, 881
TasI AATT 5 cut(s) 783, 802, 811, 849, 873
TauI GCSGC 3 cut(s) 109, 369, 378
TfiI GAWTC 2 cut(s) 360, 390
Tru1I TTAA 4 cut(s) 182, 578, 624, 702
Tru9I TTAA 4 cut(s) 182, 578, 624, 702
TseFI GTSAC 1 cut(s) 26
TseI GCWGC 3 cut(s) 486, 551, 728
Tsp45I GTSAC 1 cut(s) 26
TspDTI ATGAA 5 cut(s) 17, 89, 200, 705, 824
TspGWI ACGGA 4 cut(s) 43, 112, 168, 416
Vha464I CTTAAG 1 cut(s) 701
VpaK11BI GGWCC 1 cut(s) 155
XapI RAATTY 2 cut(s) 783, 811
XbaI TCTAGA 1 cut(s) 223
XceI RCATGY 2 cut(s) 76, 621
XspI CTAG 2 cut(s) 33, 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.