Rmu_sc0003942.1_g000027

Dihydrolipoyl dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003942.1
OG0022567
Physical Location & Seq
Forward (+)
111491 .. 112963
1473 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003942.1_g000027.1.cds

Sequence Viewer

Length: 633 bp
atgcaggcctcgctttctctccctactctctcccccaccattcccagatcaaactacgtcgttcagtccacgctgcctctcgctccctccaagccgatcaatctccgcttctgcggtctccggcgagaggccttcgggttctcctcgctcggccagtccgactcgcaccgtttccggctcgcggcggcttcgcggtccaagaaggtttcggcggccttgtctgataatggaagccctccgaagtcgttcgactacgacttgctcatcatcggagccggcgtcggcggccacggcgctgctctccacgccgtcgagaagggtctgaaaaccgccatcgtagagggagatgtggtgggtggaacatgtgttaacagaggatgtgttccatctaaagctcttcttgcagttagtggtcggatgcgggagcttcaaaatgaacatcaccttaaggctctgggtttgcaggtttcagcagctggttatgacagacagggagtggccgatcatgcaaacaatcttgcatcaaaaatccgttctagtttgaccaattcaatgaagtcgcttggagtggacatactaacaggttttggtacaatcctggtaagtcatacgtatgattttgtggcaacatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004148 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006082 GO:0006084 GO:0006085 GO:0006086 GO:0006090 GO:0006139 GO:0006163 GO:0006164 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009259 GO:0009260 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0009987 GO:0016491 GO:0016651 GO:0016667 GO:0016668 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019752 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032787 GO:0033865 GO:0033866 GO:0033875 GO:0034030 GO:0034032 GO:0034033 GO:0034641 GO:0034654 GO:0035383 GO:0035384 GO:0042221 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046390 GO:0046483 GO:0046685 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0055114 GO:0070013 GO:0071616 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00020 ko00260 ko00280 ko00620 ko00630 ko00640 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00260 map00280 map00620 map00630 map00640 map01100 map01110 map01120 map01130 map01200
Pfam Domains
Protein Families
Pyr_redox_2 Pyr_redox_dim

Protein Analysis

210

Amino Acids

22.03

Weight (kDa)

9.67

Isoelectric Point (pI)

37.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022567)

Species Orthologous Gene IDs
pyrus_communis pycom16g13240
rosa_multiflora Rmu_co8199018.1_g000001 Rmu_sc0003885.1_g000007 Rmu_sc0003942.1_g000027

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 454
AccII CGCG 2 cut(s) 182, 193
AciI CCGC 9 cut(s) 106, 114, 182, 185, 193, 212, 285, 330, 421
AcoI YGGCCR 3 cut(s) 151, 286, 498
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 1 cut(s) 592
AfiI CCNNNNNNNGG 2 cut(s) 127, 181
AflII CTTAAG 1 cut(s) 446
AflIII ACRYGT 1 cut(s) 362
AgsI TTSAA 2 cut(s) 431, 552
AjnI CCWGG 1 cut(s) 597
AluBI AGCT 3 cut(s) 395, 427, 476
AluI AGCT 3 cut(s) 395, 427, 476
Alw26I GTCTC 1 cut(s) 122
AlwNI CAGNNNCTG 1 cut(s) 476
AoxI GGCC 6 cut(s) 6, 129, 151, 213, 286, 498
ApeKI GCWGC 3 cut(s) 73, 296, 473
Asp700I GAANNNNTTC 1 cut(s) 245
AspLEI GCGC 1 cut(s) 296
AspS9I GGNCC 1 cut(s) 195
AsuHPI GGTGA 1 cut(s) 434
AvaII GGWCC 1 cut(s) 195
BbvI GCAGC 3 cut(s) 60, 283, 485
BccI CCATC 2 cut(s) 341, 394
BceAI ACGGC 2 cut(s) 293, 307
BciT130I CCWGG 1 cut(s) 599
BcoDI GTCTC 1 cut(s) 122
BfaI CTAG 1 cut(s) 537
BfoI RGCGCY 1 cut(s) 297
BfrI CTTAAG 1 cut(s) 446
BfuAI ACCTGC 1 cut(s) 454
BisI GCNGC 7 cut(s) 74, 183, 186, 213, 286, 297, 474
BlsI GCNGC 7 cut(s) 75, 184, 187, 214, 287, 298, 475
Bme1390I CCNGG 1 cut(s) 599
Bme18I GGWCC 1 cut(s) 195
BmgT120I GGNCC 1 cut(s) 195
BmiI GGNNCC 1 cut(s) 274
BmrFI CCNGG 1 cut(s) 599
BmsI GCATC 2 cut(s) 408, 530
BsaAI YACGTR 1 cut(s) 612
BsaHI GRCGYC 1 cut(s) 279
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 1 cut(s) 289
BsaXI ACNNNNNCTCC 4 cut(s) 264, 294, 336, 366
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 181
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 1 cut(s) 154
BseBI CCWGG 1 cut(s) 599
BseDI CCNNGG 1 cut(s) 289
BseGI GGATG 2 cut(s) 383, 423
BseLI CCNNNNNNNGG 2 cut(s) 127, 181
BseNI ACTGG 1 cut(s) 154
BseRI GAGGAG 1 cut(s) 133
BseXI GCAGC 3 cut(s) 60, 283, 485
Bsh1236I CGCG 2 cut(s) 182, 193
BshFI GGCC 6 cut(s) 8, 131, 153, 215, 288, 500
BsiSI CCGG 3 cut(s) 121, 175, 276
BslI CCNNNNNNNGG 2 cut(s) 127, 181
BsmAI GTCTC 1 cut(s) 122
BsnI GGCC 6 cut(s) 8, 131, 153, 215, 288, 500
Bso31I GGTCTC 1 cut(s) 122
Bsp143I GATC 3 cut(s) 47, 96, 502
BspACI CCGC 9 cut(s) 106, 114, 182, 185, 193, 212, 285, 330, 421
BspANI GGCC 6 cut(s) 8, 131, 153, 215, 288, 500
BspFNI CGCG 2 cut(s) 182, 193
BspLI GGNNCC 1 cut(s) 274
BspMI ACCTGC 1 cut(s) 454
BspQI GCTCTTC 1 cut(s) 402
BspTI CTTAAG 1 cut(s) 446
BspTNI GGTCTC 1 cut(s) 122
BsrFI RCCGGY 1 cut(s) 275
BsrI ACTGG 1 cut(s) 154
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 1 cut(s) 289
BssMI GATC 3 cut(s) 47, 96, 502
BssNI GRCGYC 1 cut(s) 279
Bst2UI CCWGG 1 cut(s) 599
Bst4CI ACNGT 1 cut(s) 170
Bst6I CTCTTC 1 cut(s) 402
BstACI GRCGYC 1 cut(s) 279
BstAFI CTTAAG 1 cut(s) 446
BstBAI YACGTR 1 cut(s) 612
BstC8I GCNNGC 3 cut(s) 6, 180, 277
BstDSI CCRYGG 1 cut(s) 289
BstF5I GGATG 2 cut(s) 383, 423
BstFNI CGCG 2 cut(s) 182, 193
BstH2I RGCGCY 1 cut(s) 297
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 3 cut(s) 50, 99, 505
BstMAI GTCTC 1 cut(s) 122
BstMBI GATC 3 cut(s) 47, 96, 502
BstMWI GCNNNNNNNGC 6 cut(s) 10, 285, 291, 305, 401, 506
BstNI CCWGG 1 cut(s) 599
BstNSI RCATGY 1 cut(s) 366
BstSCI CCNGG 1 cut(s) 597
BstSNI TACGTA 1 cut(s) 612
BstUI CGCG 2 cut(s) 182, 193
BstV1I GCAGC 3 cut(s) 60, 283, 485
BsuRI GGCC 6 cut(s) 8, 131, 153, 215, 288, 500
BtgI CCRYGG 1 cut(s) 289
BtsCI GGATG 2 cut(s) 383, 423
BveI ACCTGC 1 cut(s) 454
Cac8I GCNNGC 3 cut(s) 6, 180, 277
CaiI CAGNNNCTG 1 cut(s) 476
CfoI GCGC 1 cut(s) 296
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 1 cut(s) 195
CseI GACGC 1 cut(s) 268
Csp6I GTAC 1 cut(s) 591
CviAII CATG 3 cut(s) 363, 506, 630
CviQI GTAC 1 cut(s) 591
DpnI GATC 3 cut(s) 49, 98, 504
DpnII GATC 3 cut(s) 47, 96, 502
EaeI YGGCCR 3 cut(s) 151, 286, 498
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
Eco105I TACGTA 1 cut(s) 612
Eco147I AGGCCT 2 cut(s) 8, 131
Eco31I GGTCTC 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 195
EcoRII CCWGG 1 cut(s) 597
FaeI CATG 3 cut(s) 366, 509, 633
FaiI YATR 7 cut(s) 364, 483, 507, 575, 609, 615, 631
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FatI CATG 3 cut(s) 362, 505, 629
FauI CCCGC 1 cut(s) 414
Fnu4HI GCNGC 7 cut(s) 74, 183, 186, 213, 286, 297, 474
FokI GGATG 2 cut(s) 390, 430
Fsp4HI GCNGC 7 cut(s) 74, 183, 186, 213, 286, 297, 474
FspBI CTAG 1 cut(s) 537
GlaI GCGC 1 cut(s) 295
GluI GCNGC 7 cut(s) 74, 183, 186, 213, 286, 297, 474
HaeII RGCGCY 1 cut(s) 297
HaeIII GGCC 6 cut(s) 8, 131, 153, 215, 288, 500
HapII CCGG 3 cut(s) 121, 175, 276
HgaI GACGC 1 cut(s) 268
HhaI GCGC 1 cut(s) 296
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 3 cut(s) 366, 509, 633
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HinfI GANTC 1 cut(s) 161
HpaI GTTAAC 1 cut(s) 370
HpaII CCGG 3 cut(s) 121, 175, 276
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 3 cut(s) 69, 370, 571
Hpy188I TCNGA 6 cut(s) 160, 223, 240, 272, 324, 417
Hpy188III TCNNGA 1 cut(s) 313
Hpy8I GTNNAC 3 cut(s) 69, 370, 571
Hpy99I CGWCG 3 cut(s) 62, 284, 314
HpyAV CCTTC 3 cut(s) 142, 196, 310
HpyCH4III ACNGT 1 cut(s) 170
HpyCH4IV ACGT 2 cut(s) 57, 611
HpyCH4V TGCA 5 cut(s) 4, 404, 463, 509, 521
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 285, 291, 305, 401, 506
HpySE526I ACGT 2 cut(s) 57, 611
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 3 cut(s) 366, 509, 633
HspAI GCGC 1 cut(s) 294
KroI GCCGGC 1 cut(s) 275
KroNI GCCGGC 1 cut(s) 277
KspAI GTTAAC 1 cut(s) 370
Kzo9I GATC 3 cut(s) 47, 96, 502
LguI GCTCTTC 1 cut(s) 402
LmnI GCTCC 3 cut(s) 88, 272, 424
Lsp1109I GCAGC 3 cut(s) 60, 283, 485
LweI GCATC 2 cut(s) 408, 530
MaeI CTAG 1 cut(s) 537
MaeII ACGT 2 cut(s) 57, 611
MalI GATC 3 cut(s) 49, 98, 504
MboI GATC 3 cut(s) 47, 96, 502
MboII GAAGA 1 cut(s) 389
MluCI AATT 1 cut(s) 547
MlyI GAGTC 1 cut(s) 155
MmeI TCCRAC 2 cut(s) 183, 395
MnlI CCTC 8 cut(s) 19, 87, 97, 121, 154, 246, 334, 368
MroNI GCCGGC 1 cut(s) 275
MroXI GAANNNNTTC 1 cut(s) 245
MseI TTAA 2 cut(s) 369, 447
MslI CAYNNNNRTG 1 cut(s) 612
MspA1I CMGCKG 1 cut(s) 476
MspCI CTTAAG 1 cut(s) 446
MspI CCGG 3 cut(s) 121, 175, 276
MspR9I CCNGG 1 cut(s) 599
MvaI CCWGG 1 cut(s) 599
MvnI CGCG 2 cut(s) 182, 193
MwoI GCNNNNNNNGC 6 cut(s) 10, 285, 291, 305, 401, 506
NaeI GCCGGC 1 cut(s) 277
NdeII GATC 3 cut(s) 47, 96, 502
NgoMIV GCCGGC 1 cut(s) 275
NlaIII CATG 3 cut(s) 366, 509, 633
NlaIV GGNNCC 1 cut(s) 274
NmeAIII GCCGAG 1 cut(s) 129
NspI RCATGY 1 cut(s) 366
PceI AGGCCT 2 cut(s) 8, 131
PciI ACATGT 1 cut(s) 362
PciSI GCTCTTC 1 cut(s) 402
PcsI WCGNNNNNNNCGW 2 cut(s) 156, 276
PdiI GCCGGC 1 cut(s) 277
PdmI GAANNNNTTC 1 cut(s) 245
PkrI GCNGC 7 cut(s) 75, 184, 187, 214, 287, 298, 475
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
Ppu21I YACGTR 1 cut(s) 612
PscI ACATGT 1 cut(s) 362
Psp6I CCWGG 1 cut(s) 597
PspGI CCWGG 1 cut(s) 597
PspN4I GGNNCC 1 cut(s) 274
PspPI GGNCC 1 cut(s) 195
PstNI CAGNNNCTG 1 cut(s) 476
PvuII CAGCTG 1 cut(s) 476
RsaI GTAC 1 cut(s) 592
RsaNI GTAC 1 cut(s) 591
RseI CAYNNNNRTG 1 cut(s) 612
SapI GCTCTTC 1 cut(s) 402
SaqAI TTAA 2 cut(s) 369, 447
SatI GCNGC 7 cut(s) 74, 183, 186, 213, 286, 297, 474
Sau3AI GATC 3 cut(s) 47, 96, 502
Sau96I GGNCC 1 cut(s) 195
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 1 cut(s) 599
SetI ASST 9 cut(s) 60, 207, 397, 429, 447, 468, 478, 586, 614
SfaNI GCATC 2 cut(s) 408, 530
SinI GGWCC 1 cut(s) 195
SmiMI CAYNNNNRTG 1 cut(s) 612
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
SnaBI TACGTA 1 cut(s) 612
Sse9I AATT 1 cut(s) 547
SseBI AGGCCT 2 cut(s) 8, 131
SsiI CCGC 9 cut(s) 106, 114, 182, 185, 193, 212, 285, 330, 421
SspMI CTAG 1 cut(s) 537
StuI AGGCCT 2 cut(s) 8, 131
StyD4I CCNGG 1 cut(s) 597
TaaI ACNGT 1 cut(s) 170
TaiI ACGT 2 cut(s) 60, 614
TaqI TCGA 2 cut(s) 249, 312
TasI AATT 1 cut(s) 547
TauI GCSGC 4 cut(s) 185, 188, 215, 288
Tru1I TTAA 2 cut(s) 369, 447
Tru9I TTAA 2 cut(s) 369, 447
TseI GCWGC 3 cut(s) 73, 296, 473
TspDTI ATGAA 2 cut(s) 450, 569
TspGWI ACGGA 1 cut(s) 521
Vha464I CTTAAG 1 cut(s) 446
VpaK11BI GGWCC 1 cut(s) 195
XceI RCATGY 1 cut(s) 366
XmnI GAANNNNTTC 1 cut(s) 245
XspI CTAG 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.