pycom16g19460

Monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0017080
Physical Location & Seq
Forward (+)
16558875 .. 16561082
2208 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g19460.2

Sequence Viewer

Length: 1506 bp
ATGACCCTCAAAGGCTCAAACCATCATTTTCAAATCCCAGTCACATGCTTAGCTCTCAAGGCCAAGAACCCTCTCTCTCTATTTAAACCTCTCCATCCCTCTCTCCCCTCTCACACATCACTTCCTTCCAAAACATCTAACATTCCATTCTCTCTCTCTCTCTCTCTCTCGCTCTCCCCCTCCATTCCCAAAACACCAACTCTCCAATTTTCACTCTTTCTAATTTTGTGCAACAAGTACTCCTGTCTCCCTCACCACATACCCCAAATGGTTCACCAATCATTTGACCAAGATATTGATCTTTTTGCCAGGAAGTGCATTTGGGTGAATGGTCCTGTAATTGTAGGGGCAGGTCCTTCAGGCCTAGCAGTTGGTGCTGGCCTTAAAGAACAAGGCGTCCCCTTTATTGTACTAGAAAGACGCCTCAAACTTCACCTCCCCAAGCAATACTGCCAACTACCCAATTTCCCATTCCCTGACGACTTCCCAGAATACCCCACAAAAGACCAGTTCATCAACTACCTAGAGTCCTATGCACAACACTTTGACATAAGCCCAAATTTCAATGAGACTGTCCAGTCTGCCAAGTATGATGAAACCTTTGGTCATTGGAGGGTCAAGACGAGTATTTCAACGAGCGACTCAAACCCTTCTTCCTTTGCCGAAGTTGAGTACATCTGCCGGTGGCTCGTGGTGGCAACTGGAGAGAATTCGGAGAAAGTAGTGCCAGAATTCGAAGGCTTGGAAGAGTTTAAAGGGAATGTCGTACATGCCTGTGACTACAAGTCCGGCCTCGCTTACCGTGGCAAGCAAGTACTAGTTGTCGGATGCGGAAATTCAGGCATGGAAGTCTCTCTTGATCTTTGCAATCACAATGCAAGTCCTTCAATGGTGGTTCGAAGTTCGGTTCATGTCTTGCCAAGGGAGATTTTTGGAAAGTCAACCTTTGAATTGGCAGTTTTGTTGATGAAATGGCTGCCACTCTGGCTTGCTGACAAGATATTGCTGATGCTGGCATGGCTACTTTTTGGAAATCTTGAAAAATATGGCCTGAAAAGGCCATCTTTAGGTCCTTTGGAGCTCAAAAACATTTCAGGGAAAACTCCAGTGTTGGATACTGGTGCCTTGAAAAAAGTAAGATCTGGTAAGATCAAGGTGGTTCCTGGAATCTCAAGGTTCTCTCATGACAGAGTTGAACTTGTTGATGGACAAATTCTTGAGATCGATTCTGTTGTTTTGGCAACTGGGTATCGGAGCAATGTTCCTTCATGGCTCAAGGAAAATGAATTCTTTTCACGAGAAGGAATTCCTAAGAATGCATTTCCAAATGGGTGGAAAGGGAATGCAGGACTATATGCAGTAGGGTTTACAAGGAGAGGACTATCTGGAGCCTCATTGGATGCCATTGGTGTGTCACAGGACATTGCCAAGAGCTGGAAAGAAGAGACCAAGCAGAAGAGAAAATCTGTTGCAGCTCGCCATAGGAGATGCATTTCACATTTTTAA

Protein Analysis

502

Amino Acids

55.73

Weight (kDa)

9.15

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04610 AT2G33230
fragaria_vesca FvH4_4g05280
malus_domestica MD16G1232600.v1.1
prunus_persica Prupe.1G054300_v2.0.a1
pyrus_communis pycom16g19460
rosa_chinensis RchiOBHm_Chr4g0396251
rosa_laevigata RLG00000009557
rosa_multiflora Rmu_sc0006325.1_g000058
rosa_roxburghii Rroxscaffold_5G00341070
rosa_rugosa Rorug03G0350800
rosa_samantha Rh4DG062600
rosa_wichuraiana Rw4G005430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 577
Acc36I ACCTGC 1 cut(s) 341
AccB1I GGYRCC 1 cut(s) 1121
AccB7I CCANNNNNTGG 1 cut(s) 1434
AciI CCGC 1 cut(s) 831
AcsI RAATTY 7 cut(s) 559, 709, 731, 835, 1212, 1286, 1305
AcuI CTGAAG 1 cut(s) 342
AcyI GRCGYC 2 cut(s) 396, 421
AfaI GTAC 5 cut(s) 239, 411, 674, 768, 816
AfiI CCNNNNNNNGG 2 cut(s) 1067, 1434
AgsI TTSAA 8 cut(s) 32, 565, 633, 888, 950, 1040, 1129, 1196
AhdI GACNNNNNGTC 1 cut(s) 784
AhlI ACTAGT 1 cut(s) 817
AjnI CCWGG 2 cut(s) 308, 1162
AjuI GAANNNNNNNTTGG 2 cut(s) 455, 487
AloI GAACNNNNNNTCC 2 cut(s) 381, 413
AluBI AGCT 4 cut(s) 53, 1081, 1434, 1475
AluI AGCT 4 cut(s) 53, 1081, 1434, 1475
Alw21I GWGCWC 1 cut(s) 1083
Alw26I GTCTC 4 cut(s) 251, 563, 856, 1439
AoxI GGCC 6 cut(s) 60, 361, 379, 790, 1048, 1058
ApeKI GCWGC 2 cut(s) 976, 1472
ApoI RAATTY 7 cut(s) 559, 709, 731, 835, 1212, 1286, 1305
Asp700I GAANNNNTTC 1 cut(s) 1305
AspS9I GGNCC 3 cut(s) 332, 353, 1070
AsuHPI GGTGA 4 cut(s) 245, 266, 337, 425
AsuII TTCGAA 2 cut(s) 735, 898
AvaII GGWCC 3 cut(s) 332, 353, 1070
BanI GGYRCC 1 cut(s) 1121
BanII GRGCYC 1 cut(s) 1083
BauI CACGAG 2 cut(s) 689, 1296
Bbv12I GWGCWC 1 cut(s) 1083
BbvI GCAGC 2 cut(s) 963, 1484
BccI CCATC 4 cut(s) 30, 102, 1069, 1199
BciT130I CCWGG 2 cut(s) 310, 1164
BciVI GTATCC 1 cut(s) 1108
BcoDI GTCTC 4 cut(s) 251, 563, 856, 1439
BcuI ACTAGT 1 cut(s) 817
BfaI CTAG 4 cut(s) 365, 413, 524, 818
BfuAI ACCTGC 1 cut(s) 341
BfuI GTATCC 1 cut(s) 1108
BglI GCCNNNNNGGC 1 cut(s) 985
BglII AGATCT 1 cut(s) 1139
BisI GCNGC 2 cut(s) 977, 1473
BlpI GCTNAGC 1 cut(s) 49
BlsI GCNGC 2 cut(s) 978, 1474
BmcAI AGTACT 2 cut(s) 239, 816
Bme1390I CCNGG 2 cut(s) 310, 1164
Bme18I GGWCC 3 cut(s) 332, 353, 1070
BmeRI GACNNNNNGTC 1 cut(s) 784
BmgT120I GGNCC 3 cut(s) 332, 353, 1070
BmiI GGNNCC 3 cut(s) 1123, 1161, 1390
BmrFI CCNGG 2 cut(s) 310, 1164
BmrI ACTGGG 2 cut(s) 32, 1254
BmsI GCATC 4 cut(s) 818, 999, 1390, 1478
BmuI ACTGGG 2 cut(s) 32, 1254
BpmI CTGGAG 3 cut(s) 723, 1089, 1407
Bpu1102I GCTNAGC 1 cut(s) 49
Bpu14I TTCGAA 2 cut(s) 735, 898
BpuEI CTTGAG 4 cut(s) 41, 1156, 1238, 1259
Bsa29I ATCGAT 1 cut(s) 1224
BsaBI GATNNNNATC 1 cut(s) 297
BsaHI GRCGYC 2 cut(s) 396, 421
BsaI GGTCTC 1 cut(s) 1439
BsaJI CCNNGG 2 cut(s) 802, 920
BsaXI ACNNNNNCTCC 6 cut(s) 186, 216, 224, 254, 420, 450
Bsc4I CCNNNNNNNGG 2 cut(s) 1067, 1434
Bse118I RCCGGY 1 cut(s) 681
Bse1I ACTGG 7 cut(s) 38, 508, 577, 706, 1106, 1123, 1249
Bse3DI GCAATG 2 cut(s) 1264, 1422
Bse8I GATNNNNATC 1 cut(s) 297
BseBI CCWGG 2 cut(s) 310, 1164
BseCI ATCGAT 1 cut(s) 1224
BseDI CCNNGG 2 cut(s) 802, 920
BseGI GGATG 3 cut(s) 94, 833, 1405
BseJI GATNNNNATC 1 cut(s) 297
BseLI CCNNNNNNNGG 2 cut(s) 1067, 1434
BseMI GCAATG 2 cut(s) 1264, 1422
BseNI ACTGG 7 cut(s) 38, 508, 577, 706, 1106, 1123, 1249
BseXI GCAGC 2 cut(s) 963, 1484
BshFI GGCC 6 cut(s) 62, 363, 381, 792, 1050, 1060
BshNI GGYRCC 1 cut(s) 1121
BshVI ATCGAT 1 cut(s) 1224
BsiHKAI GWGCWC 1 cut(s) 1083
BsiSI CCGG 2 cut(s) 682, 789
BslFI GGGAC 1 cut(s) 383
BslI CCNNNNNNNGG 2 cut(s) 1067, 1434
BsmAI GTCTC 4 cut(s) 251, 563, 856, 1439
BsmFI GGGAC 1 cut(s) 383
BsmI GAATGC 2 cut(s) 1321, 1348
BsnI GGCC 6 cut(s) 62, 363, 381, 792, 1050, 1060
Bso31I GGTCTC 1 cut(s) 1439
Bsp119I TTCGAA 2 cut(s) 735, 898
Bsp1286I GDGCHC 1 cut(s) 1083
Bsp143I GATC 5 cut(s) 298, 859, 1139, 1149, 1221
Bsp1720I GCTNAGC 1 cut(s) 49
BspACI CCGC 1 cut(s) 831
BspANI GGCC 6 cut(s) 62, 363, 381, 792, 1050, 1060
BspDI ATCGAT 1 cut(s) 1224
BspHI TCATGA 1 cut(s) 1183
BspLI GGNNCC 3 cut(s) 1123, 1161, 1390
BspMI ACCTGC 1 cut(s) 341
BspT104I TTCGAA 2 cut(s) 735, 898
BspT107I GGYRCC 1 cut(s) 1121
BspTNI GGTCTC 1 cut(s) 1439
BsrDI GCAATG 2 cut(s) 1264, 1422
BsrFI RCCGGY 1 cut(s) 681
BsrI ACTGG 7 cut(s) 38, 508, 577, 706, 1106, 1123, 1249
BssAI RCCGGY 1 cut(s) 681
BssECI CCNNGG 2 cut(s) 802, 920
BssMI GATC 5 cut(s) 298, 859, 1139, 1149, 1221
BssNI GRCGYC 2 cut(s) 396, 421
BssSI CACGAG 2 cut(s) 689, 1296
BssT1I CCWWGG 1 cut(s) 920
Bst2BI CACGAG 2 cut(s) 689, 1296
Bst2UI CCWGG 2 cut(s) 310, 1164
Bst4CI ACNGT 2 cut(s) 574, 803
Bst6I CTCTTC 3 cut(s) 741, 1437, 1451
BstACI GRCGYC 2 cut(s) 396, 421
BstAPI GCANNNNNTGC 1 cut(s) 374
BstBI TTCGAA 2 cut(s) 735, 898
BstC8I GCNNGC 5 cut(s) 379, 809, 990, 1014, 1477
BstDEI CTNAG 2 cut(s) 49, 1311
BstDSI CCRYGG 1 cut(s) 802
BstF5I GGATG 3 cut(s) 94, 833, 1405
BstKTI GATC 5 cut(s) 301, 862, 1142, 1152, 1224
BstMAI GTCTC 4 cut(s) 251, 563, 856, 1439
BstMBI GATC 5 cut(s) 298, 859, 1139, 1149, 1221
BstMWI GCNNNNNNNGC 4 cut(s) 59, 374, 985, 1018
BstNI CCWGG 2 cut(s) 310, 1164
BstNSI RCATGY 2 cut(s) 48, 773
BstSCI CCNGG 2 cut(s) 308, 1162
BstV1I GCAGC 2 cut(s) 963, 1484
BstX2I RGATCY 1 cut(s) 1139
BstXI CCANNNNNNTGG 1 cut(s) 1332
BstYI RGATCY 1 cut(s) 1139
Bsu15I ATCGAT 1 cut(s) 1224
BsuI GTATCC 1 cut(s) 1108
BsuRI GGCC 6 cut(s) 62, 363, 381, 792, 1050, 1060
BsuTUI ATCGAT 1 cut(s) 1224
BtgI CCRYGG 1 cut(s) 802
BtsCI GGATG 3 cut(s) 94, 833, 1405
BtsIMutI CAGTG 1 cut(s) 1113
BveI ACCTGC 1 cut(s) 341
Cac8I GCNNGC 5 cut(s) 379, 809, 990, 1014, 1477
CciI TCATGA 1 cut(s) 1183
Cfr10I RCCGGY 1 cut(s) 681
Cfr13I GGNCC 3 cut(s) 332, 353, 1070
ClaI ATCGAT 1 cut(s) 1224
CseI GACGC 2 cut(s) 385, 429
Csp6I GTAC 5 cut(s) 238, 410, 673, 767, 815
CviAII CATG 7 cut(s) 45, 770, 844, 911, 1017, 1184, 1269
CviQI GTAC 5 cut(s) 238, 410, 673, 767, 815
DdeI CTNAG 2 cut(s) 49, 1311
DpnI GATC 5 cut(s) 300, 861, 1141, 1151, 1223
DpnII GATC 5 cut(s) 298, 859, 1139, 1149, 1221
DraI TTTAAA 2 cut(s) 85, 754
DrdI GACNNNNNNGTC 1 cut(s) 577
DriI GACNNNNNGTC 1 cut(s) 784
DseDI GACNNNNNNGTC 1 cut(s) 577
Eam1104I CTCTTC 3 cut(s) 741, 1437, 1451
Eam1105I GACNNNNNGTC 1 cut(s) 784
EarI CTCTTC 3 cut(s) 741, 1437, 1451
Ecl136II GAGCTC 1 cut(s) 1081
Eco130I CCWWGG 1 cut(s) 920
Eco147I AGGCCT 1 cut(s) 363
Eco24I GRGCYC 1 cut(s) 1083
Eco31I GGTCTC 1 cut(s) 1439
Eco47I GGWCC 3 cut(s) 332, 353, 1070
Eco53kI GAGCTC 1 cut(s) 1081
Eco57I CTGAAG 1 cut(s) 342
EcoICRI GAGCTC 1 cut(s) 1081
EcoO109I RGGNCCY 2 cut(s) 353, 1070
EcoRI GAATTC 4 cut(s) 709, 731, 1286, 1305
EcoRII CCWGG 2 cut(s) 308, 1162
EcoT14I CCWWGG 1 cut(s) 920
EcoT22I ATGCAT 2 cut(s) 1321, 1493
EcoT38I GRGCYC 1 cut(s) 1083
ErhI CCWWGG 1 cut(s) 920
FaeI CATG 7 cut(s) 48, 773, 847, 914, 1020, 1187, 1272
FalI AAGNNNNNCTT 6 cut(s) 840, 872, 929, 961, 1048, 1080
FaqI GGGAC 1 cut(s) 383
FatI CATG 7 cut(s) 44, 769, 843, 910, 1016, 1183, 1268
Fnu4HI GCNGC 2 cut(s) 977, 1473
FokI GGATG 3 cut(s) 81, 840, 1412
FriOI GRGCYC 1 cut(s) 1083
Fsp4HI GCNGC 2 cut(s) 977, 1473
FspBI CTAG 4 cut(s) 365, 413, 524, 818
GluI GCNGC 2 cut(s) 977, 1473
GsuI CTGGAG 3 cut(s) 723, 1089, 1407
HaeIII GGCC 6 cut(s) 62, 363, 381, 792, 1050, 1060
HapII CCGG 2 cut(s) 682, 789
HgaI GACGC 2 cut(s) 385, 429
Hin1I GRCGYC 2 cut(s) 396, 421
Hin1II CATG 7 cut(s) 48, 773, 847, 914, 1020, 1187, 1272
HincII GTYRAC 1 cut(s) 942
HindII GTYRAC 1 cut(s) 942
HinfI GANTC 4 cut(s) 527, 641, 1167, 1226
HpaII CCGG 2 cut(s) 682, 789
HphI GGTGA 4 cut(s) 245, 266, 337, 425
Hpy166II GTNNAC 3 cut(s) 274, 942, 1368
Hpy188I TCNGA 3 cut(s) 715, 827, 1254
Hpy188III TCNNGA 7 cut(s) 619, 857, 1037, 1184, 1217, 1296, 1386
Hpy8I GTNNAC 3 cut(s) 274, 942, 1368
HpyAV CCTTC 7 cut(s) 135, 366, 660, 731, 894, 1275, 1295
HpyCH4III ACNGT 2 cut(s) 574, 803
HpyF10VI GCNNNNNNNGC 4 cut(s) 59, 374, 985, 1018
HpyF3I CTNAG 2 cut(s) 49, 1311
Hsp92I GRCGYC 2 cut(s) 396, 421
Hsp92II CATG 7 cut(s) 48, 773, 847, 914, 1020, 1187, 1272
Kzo9I GATC 5 cut(s) 298, 859, 1139, 1149, 1221
LmnI GCTCC 3 cut(s) 1078, 1254, 1388
Lsp1109I GCAGC 2 cut(s) 963, 1484
LweI GCATC 4 cut(s) 818, 999, 1390, 1478
MaeI CTAG 4 cut(s) 365, 413, 524, 818
MaeIII GTNAC 3 cut(s) 40, 776, 1413
MalI GATC 5 cut(s) 300, 861, 1141, 1151, 1223
MboI GATC 5 cut(s) 298, 859, 1139, 1149, 1221
MboII GAAGA 4 cut(s) 645, 758, 1454, 1468
MflI RGATCY 1 cut(s) 1139
MhlI GDGCHC 1 cut(s) 1083
MlyI GAGTC 2 cut(s) 536, 635
MmeI TCCRAC 2 cut(s) 805, 1092
Mph1103I ATGCAT 2 cut(s) 1321, 1493
MroXI GAANNNNTTC 1 cut(s) 1305
MseI TTAA 4 cut(s) 84, 384, 753, 1504
MslI CAYNNNNRTG 3 cut(s) 323, 774, 1409
MspI CCGG 2 cut(s) 682, 789
MspR9I CCNGG 2 cut(s) 310, 1164
Mva1269I GAATGC 2 cut(s) 1321, 1348
MvaI CCWGG 2 cut(s) 310, 1164
MwoI GCNNNNNNNGC 4 cut(s) 59, 374, 985, 1018
NdeII GATC 5 cut(s) 298, 859, 1139, 1149, 1221
NlaIII CATG 7 cut(s) 48, 773, 847, 914, 1020, 1187, 1272
NlaIV GGNNCC 3 cut(s) 1123, 1161, 1390
NmuCI GTSAC 3 cut(s) 40, 776, 1413
NsiI ATGCAT 2 cut(s) 1321, 1493
NspI RCATGY 2 cut(s) 48, 773
NspV TTCGAA 2 cut(s) 735, 898
PagI TCATGA 1 cut(s) 1183
PceI AGGCCT 1 cut(s) 363
PctI GAATGC 2 cut(s) 1321, 1348
PdmI GAANNNNTTC 1 cut(s) 1305
PfeI GAWTC 2 cut(s) 1167, 1226
PflMI CCANNNNNTGG 1 cut(s) 1434
PfoI TCCNGGA 1 cut(s) 1162
PkrI GCNGC 2 cut(s) 978, 1474
PleI GAGTC 2 cut(s) 535, 635
PpsI GAGTC 2 cut(s) 535, 635
PpuMI RGGWCCY 2 cut(s) 353, 1070
Psp124BI GAGCTC 1 cut(s) 1083
Psp5II RGGWCCY 2 cut(s) 353, 1070
Psp6I CCWGG 2 cut(s) 308, 1162
PspGI CCWGG 2 cut(s) 308, 1162
PspN4I GGNNCC 3 cut(s) 1123, 1161, 1390
PspPI GGNCC 3 cut(s) 332, 353, 1070
PspPPI RGGWCCY 2 cut(s) 353, 1070
PsuI RGATCY 1 cut(s) 1139
RsaI GTAC 5 cut(s) 239, 411, 674, 768, 816
RsaNI GTAC 5 cut(s) 238, 410, 673, 767, 815
RseI CAYNNNNRTG 3 cut(s) 323, 774, 1409
SacI GAGCTC 1 cut(s) 1083
SaqAI TTAA 4 cut(s) 84, 384, 753, 1504
SatI GCNGC 2 cut(s) 977, 1473
Sau3AI GATC 5 cut(s) 298, 859, 1139, 1149, 1221
Sau96I GGNCC 3 cut(s) 332, 353, 1070
ScaI AGTACT 2 cut(s) 239, 816
SchI GAGTC 2 cut(s) 536, 635
ScrFI CCNGG 2 cut(s) 310, 1164
SduI GDGCHC 1 cut(s) 1083
SfaNI GCATC 4 cut(s) 818, 999, 1390, 1478
SfuI TTCGAA 2 cut(s) 735, 898
SinI GGWCC 3 cut(s) 332, 353, 1070
SmiMI CAYNNNNRTG 3 cut(s) 323, 774, 1409
SmlI CTYRAG 4 cut(s) 56, 1171, 1217, 1274
SmoI CTYRAG 4 cut(s) 56, 1171, 1217, 1274
SpeI ACTAGT 1 cut(s) 817
SseBI AGGCCT 1 cut(s) 363
SsiI CCGC 1 cut(s) 831
SspMI CTAG 4 cut(s) 365, 413, 524, 818
SstI GAGCTC 1 cut(s) 1083
StuI AGGCCT 1 cut(s) 363
StyD4I CCNGG 2 cut(s) 308, 1162
StyI CCWWGG 1 cut(s) 920
TaaI ACNGT 2 cut(s) 574, 803
TaqI TCGA 3 cut(s) 735, 898, 1224
TatI WGTACW 4 cut(s) 237, 409, 672, 814
TfiI GAWTC 2 cut(s) 1167, 1226
Tru1I TTAA 4 cut(s) 84, 384, 753, 1504
Tru9I TTAA 4 cut(s) 84, 384, 753, 1504
TscAI CASTG 1 cut(s) 1113
TseFI GTSAC 3 cut(s) 40, 776, 1413
TseI GCWGC 2 cut(s) 976, 1472
Tsp45I GTSAC 3 cut(s) 40, 776, 1413
TspDTI ATGAA 6 cut(s) 502, 609, 899, 983, 1257, 1299
TspRI CASTG 1 cut(s) 1113
Van91I CCANNNNNTGG 1 cut(s) 1434
VpaK11BI GGWCC 3 cut(s) 332, 353, 1070
XapI RAATTY 7 cut(s) 559, 709, 731, 835, 1212, 1286, 1305
XceI RCATGY 2 cut(s) 48, 773
XmnI GAANNNNTTC 1 cut(s) 1305
XspI CTAG 4 cut(s) 365, 413, 524, 818
ZrmI AGTACT 2 cut(s) 239, 816
Zsp2I ATGCAT 2 cut(s) 1321, 1493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.