pycom16g25490

Protein BUD31 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0024707
Physical Location & Seq
Forward (+)
27451567 .. 27451866
300 bp
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UTR
Exon/CDS
Intron
pycom16g25490.1

Sequence Viewer

Length: 186 bp
ATGCCCAAGGTGAAGACTAACCGAGTGAAATACCCAGAAGGTTGGGAACTGATTGAGCCTACTCTTCGCGAGCTACAAGCTAAGATGAGAGAAGCTGAGAATGATACTCATGATGGTAAGAGAAAATGTGAGACCTTATGGCCTATTTTCAAAATTGCACATCAAAAGAGCCGCTATATTTTTTGA

Protein Analysis

62

Amino Acids

7.39

Weight (kDa)

9.46

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BUD31 PF01125 1 - 61 2e-19 Pre-mRNA-splicing factor BUD31
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0024707)

Species Orthologous Gene IDs
pyrus_communis pycom16g25490
rosa_samantha Rh6BG152900 Rh6CG148800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 69
AciI CCGC 1 cut(s) 172
AgsI TTSAA 1 cut(s) 151
AluBI AGCT 3 cut(s) 73, 80, 95
AluI AGCT 3 cut(s) 73, 80, 95
Alw26I GTCTC 1 cut(s) 125
AoxI GGCC 1 cut(s) 140
AsuHPI GGTGA 1 cut(s) 22
BbsI GAAGAC 1 cut(s) 20
BccI CCATC 1 cut(s) 107
BcoDI GTCTC 1 cut(s) 125
BisI GCNGC 1 cut(s) 172
BlsI GCNGC 1 cut(s) 173
BpiI GAAGAC 1 cut(s) 20
BsaI GGTCTC 1 cut(s) 125
BsaJI CCNNGG 1 cut(s) 6
BseDI CCNNGG 1 cut(s) 6
BseMII CTCAG 1 cut(s) 87
Bsh1236I CGCG 1 cut(s) 69
BshFI GGCC 1 cut(s) 142
BsmAI GTCTC 1 cut(s) 125
BsnI GGCC 1 cut(s) 142
Bso31I GGTCTC 1 cut(s) 125
Bsp68I TCGCGA 1 cut(s) 69
BspACI CCGC 1 cut(s) 172
BspANI GGCC 1 cut(s) 142
BspCNI CTCAG 1 cut(s) 88
BspFNI CGCG 1 cut(s) 69
BspHI TCATGA 1 cut(s) 109
BspTNI GGTCTC 1 cut(s) 125
BssECI CCNNGG 1 cut(s) 6
BssT1I CCWWGG 1 cut(s) 6
Bst6I CTCTTC 1 cut(s) 69
BstC8I GCNNGC 1 cut(s) 71
BstDEI CTNAG 2 cut(s) 81, 96
BstFNI CGCG 1 cut(s) 69
BstMAI GTCTC 1 cut(s) 125
BstUI CGCG 1 cut(s) 69
BstV2I GAAGAC 1 cut(s) 20
BstXI CCANNNNNNTGG 1 cut(s) 42
BsuRI GGCC 1 cut(s) 142
BtuMI TCGCGA 1 cut(s) 69
Cac8I GCNNGC 1 cut(s) 71
CciI TCATGA 1 cut(s) 109
CviAII CATG 1 cut(s) 110
CviJI RGCY 6 cut(s) 58, 73, 80, 95, 142, 171
CviKI_1 RGCY 6 cut(s) 58, 73, 80, 95, 142, 171
DdeI CTNAG 2 cut(s) 81, 96
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 6
Eco31I GGTCTC 1 cut(s) 125
EcoT14I CCWWGG 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 6
FaeI CATG 1 cut(s) 113
FaiI YATR 3 cut(s) 111, 139, 177
FatI CATG 1 cut(s) 109
Fnu4HI GCNGC 1 cut(s) 172
Fsp4HI GCNGC 1 cut(s) 172
GluI GCNGC 1 cut(s) 172
HaeIII GGCC 1 cut(s) 142
Hin1II CATG 1 cut(s) 113
HphI GGTGA 1 cut(s) 22
Hpy188III TCNNGA 2 cut(s) 68, 110
HpyAV CCTTC 1 cut(s) 32
HpyCH4V TGCA 1 cut(s) 158
HpyF3I CTNAG 2 cut(s) 81, 96
Hsp92II CATG 1 cut(s) 113
LpnPI CCDG 1 cut(s) 48
MboII GAAGA 2 cut(s) 25, 56
MluCI AATT 1 cut(s) 153
MvnI CGCG 1 cut(s) 69
NlaIII CATG 1 cut(s) 113
NruI TCGCGA 1 cut(s) 69
PagI TCATGA 1 cut(s) 109
PkrI GCNGC 1 cut(s) 173
RruI TCGCGA 1 cut(s) 69
SatI GCNGC 1 cut(s) 172
SetI ASST 6 cut(s) 12, 43, 75, 82, 97, 137
SgeI CNNG 7 cut(s) 19, 35, 47, 80, 82, 89, 122
Sse9I AATT 1 cut(s) 153
SsiI CCGC 1 cut(s) 172
StyI CCWWGG 1 cut(s) 6
TasI AATT 1 cut(s) 153
TauI GCSGC 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.