pycom17g22930
HSP70 Family

Heat shock 70 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0022574
Physical Location & Seq
Forward (+)
21351120 .. 21352799
1680 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g22930.1

Sequence Viewer

Length: 1569 bp
ATGTATTTTTTCGTTAAAGAAAACAGTATCGAGAACTTTTCGTTAAATACGTATCCCAATTCAAAATCCCTCCCAAACCCAAATCCAAATGTGGAAAGTTGCGCATTGGTTCGATGTTTCCAGATCTCTCTGGTTCCTTCCTTCTTCCCTCTTTTCTTCTTCTATACTTCTACATACCTCTCGAAATTTCTTGCCCTCCTCCTTCGTACCGGACACCCAATGCCTGCATATATGCATTGCCATGGTCTCCCAGACAGACCATCAGGCAAACAACAACAGAAAAGCTCACTGCACCCATTCAACAACCCTAATCCTTCTTTTAAATTTTTTCCTGATTTCTTCTTTTCTTATTCATGGCTTCAAAGACGCAAGACAAGGCCATCGGCATCGACCTCGGCACCACCTACAGCTGCGTCGGGGTGTGGCAGAACGACCGCGTTGAGATCGATAGCCAATGACCAGGGAAACAGGACCACCCCTTCCTATGTCGCCTTCACCGACACGGAGCGTTTGATCGGCGATGCAGCGAAGAACCAAGTCGCTATGAACCCCCAAAACACCGTTTTCGACGCCAAGAGACTCATCGGCAGGCGGTTCTCCGACCCTTCTGTCCAGAGCGACATGAAGCACTGGCCTTTCAAGGTCGTTGCTGGTCCCGGCGACAAGCCCATGATTGTGGTAAATTACAAAGGCGAAGAGAAGCAGTTTGCGGCGGAAGAGATTTCGTCAATGGTGCTGACTAAGATGAGGGAGATTGCCGAGGCTTATTTGGGTCACCCTTGCAACAACGCTGTGGTCACTGTACCTGCGTACTTCAATGACTCGCAGCGGCAGGCCACAAAGGATGCTGGCTCCATTGCCGGGCTCAATGTGATGAGGATTATCAACGAGCCTACTGCTGCTGCCATTGCTTACGGTCTTGCCATTGCTTACGGTCTTGACAAGAAGGGATCCAGTACTGGCGAGAAGAATGTTCTTATTTTCGATCTTGGTGGCGGGACTTTTGATGTTTCTTTGCTCACAATTGAGGAGGGGATCTTCGAAGTGAAGGCTACTGCTGGTGATACTCATCTTGGTGGTGAGGATTTTGACAACAGGCTTGTTAATCACTTTGTGCAGGAGTTTAAGAGAAAGCACAAGAAGGATGTTAGTAGCAACGCCAGAGCTTTGAGGAGGTTGAGGACTGCGTGCGAGAGGGCGAAAAGGACTTTGTCTTCCACCGCCCAGACCACCATTGAGATCGATTCGCTCTACGAAGGTATTGATTTCTATTCTACAATTACTAGGGCGAGATTTGAAGAGATGAATATGGATTTGTTTAGGAAGTGTATGGAACCTGTTGAAAAGTGTTTGAGGGATTCCAAAATCGACAAGAGTCGAGTTGATGAGGTTGTTTTGGTTGGTGGGTCGACGAGAATTCCCAAAGTTCAGCAGCTTTTGCAAGATTTCTTCAATGGCAAGGAATTGTGCAAGAGCATAAACCCTGACGAAGCCGTGGCTTATGGTGCTGCTGTTCAAGCTGCAATTTTAAGCGGCGACGGAAATGAGAAGGTCCAAGATCGATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko04010 ko04141 ko04144 ko04213 ko04612 ko04915 ko05134 ko05145 ko05162 ko05164 ko05169 map03040 map04010 map04141 map04144 map04213 map04612 map04915 map05134 map05145 map05162 map05164 map05169
Pfam Domains
Protein Families
HSP70

Protein Analysis

523

Amino Acids

58.07

Weight (kDa)

8.86

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 146 - 517 2.7e-169 Hsp70 protein
MreB_Mbl PF06723 259 - 504 1.5e-12 MreB/Mbl protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022574)

Species Orthologous Gene IDs
pyrus_communis pycom17g22930
rosa_chinensis RchiOBHm_Chr7g0201441
rosa_multiflora Rmu_ssc0000250.1_g000022
rosa_rugosa Rorug07G0107700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 608
Acc16I TGCGCA 1 cut(s) 103
Acc36I ACCTGC 1 cut(s) 814
AccB1I GGYRCC 1 cut(s) 397
AccI GTMKAC 1 cut(s) 1409
AccII CGCG 1 cut(s) 437
AciI CCGC 8 cut(s) 435, 592, 710, 713, 829, 996, 1221, 1533
AclWI GGATC 4 cut(s) 945, 958, 1043, 1557
AcsI RAATTY 3 cut(s) 185, 323, 1416
AcyI GRCGYC 1 cut(s) 570
AdeI CACNNNGTG 1 cut(s) 1114
AfaI GTAC 4 cut(s) 208, 804, 812, 958
AfiI CCNNNNNNNGG 1 cut(s) 861
AgsI TTSAA 9 cut(s) 63, 301, 362, 640, 817, 1298, 1343, 1453, 1517
AjnI CCWGG 1 cut(s) 459
AluBI AGCT 5 cut(s) 285, 410, 1166, 1435, 1520
AluI AGCT 5 cut(s) 285, 410, 1166, 1435, 1520
Alw26I GTCTC 2 cut(s) 251, 571
AlwI GGATC 4 cut(s) 945, 958, 1043, 1557
AoxI GGCC 3 cut(s) 377, 632, 834
ApeKI GCWGC 8 cut(s) 410, 524, 826, 899, 902, 1432, 1508, 1520
ApoI RAATTY 3 cut(s) 185, 323, 1416
AspLEI GCGC 1 cut(s) 104
AspS9I GGNCC 3 cut(s) 471, 653, 1552
AsuC2I CCSGG 2 cut(s) 657, 862
AsuHPI GGTGA 4 cut(s) 487, 767, 1073, 1091
AsuII TTCGAA 1 cut(s) 1041
AvaII GGWCC 3 cut(s) 471, 653, 1552
BamHI GGATCC 1 cut(s) 950
BanI GGYRCC 1 cut(s) 397
BanII GRGCYC 1 cut(s) 867
BbsI GAAGAC 1 cut(s) 1206
BbvI GCAGC 8 cut(s) 397, 536, 838, 886, 889, 1444, 1495, 1507
BccI CCATC 2 cut(s) 268, 388
BceAI ACGGC 1 cut(s) 1478
BcgI CGANNNNNNTGC 4 cut(s) 375, 409, 878, 912
BciT130I CCWGG 1 cut(s) 461
BciVI GTATCC 1 cut(s) 63
BcnI CCSGG 2 cut(s) 657, 862
BcoDI GTCTC 2 cut(s) 251, 571
BfaI CTAG 1 cut(s) 1284
BfmI CTRYAG 1 cut(s) 405
BfuAI ACCTGC 1 cut(s) 814
BfuI GTATCC 1 cut(s) 63
BglII AGATCT 1 cut(s) 123
BmcAI AGTACT 1 cut(s) 958
Bme1390I CCNGG 3 cut(s) 461, 657, 862
Bme18I GGWCC 3 cut(s) 471, 653, 1552
BmgT120I GGNCC 3 cut(s) 471, 653, 1552
BmiI GGNNCC 6 cut(s) 135, 399, 655, 853, 952, 1335
BmrFI CCNGG 3 cut(s) 461, 657, 862
BmsI GCATC 3 cut(s) 395, 511, 835
BoxI GACNNNNGTC 1 cut(s) 1374
BpiI GAAGAC 1 cut(s) 1206
Bpu14I TTCGAA 1 cut(s) 1041
BpuMI CCSGG 2 cut(s) 657, 862
Bsa29I ATCGAT 3 cut(s) 446, 1242, 1561
BsaAI YACGTR 1 cut(s) 51
BsaBI GATNNNNATC 1 cut(s) 1068
BsaHI GRCGYC 1 cut(s) 570
BsaI GGTCTC 1 cut(s) 251
BsaJI CCNNGG 5 cut(s) 241, 393, 460, 759, 1494
BsaWI WCCGGW 1 cut(s) 209
Bsc4I CCNNNNNNNGG 1 cut(s) 861
Bse1I ACTGG 3 cut(s) 635, 954, 964
Bse3DI GCAATG 4 cut(s) 235, 855, 906, 924
Bse8I GATNNNNATC 1 cut(s) 1068
BseBI CCWGG 1 cut(s) 461
BseCI ATCGAT 3 cut(s) 446, 1242, 1561
BseDI CCNNGG 5 cut(s) 241, 393, 460, 759, 1494
BseGI GGATG 2 cut(s) 850, 1150
BseJI GATNNNNATC 1 cut(s) 1068
BseLI CCNNNNNNNGG 1 cut(s) 861
BseMI GCAATG 4 cut(s) 235, 855, 906, 924
BseNI ACTGG 3 cut(s) 635, 954, 964
BseRI GAGGAG 3 cut(s) 188, 1043, 1186
BseXI GCAGC 8 cut(s) 397, 536, 838, 886, 889, 1444, 1495, 1507
BsgI GTGCAG 2 cut(s) 275, 1136
Bsh1236I CGCG 1 cut(s) 437
Bsh1285I CGRYCG 1 cut(s) 435
BshFI GGCC 3 cut(s) 379, 634, 836
BshNI GGYRCC 1 cut(s) 397
BshVI ATCGAT 3 cut(s) 446, 1242, 1561
BsiEI CGRYCG 1 cut(s) 435
BsiSI CCGG 3 cut(s) 210, 657, 861
BslFI GGGAC 2 cut(s) 639, 1012
BslI CCNNNNNNNGG 1 cut(s) 861
BsmAI GTCTC 2 cut(s) 251, 571
BsmFI GGGAC 2 cut(s) 639, 1012
BsnI GGCC 3 cut(s) 379, 634, 836
Bso31I GGTCTC 1 cut(s) 251
Bsp119I TTCGAA 1 cut(s) 1041
Bsp1286I GDGCHC 1 cut(s) 867
Bsp143I GATC 9 cut(s) 123, 443, 513, 950, 985, 1035, 1239, 1558, 1562
Bsp19I CCATGG 1 cut(s) 241
BspACI CCGC 8 cut(s) 435, 592, 710, 713, 829, 996, 1221, 1533
BspANI GGCC 3 cut(s) 379, 634, 836
BspDI ATCGAT 3 cut(s) 446, 1242, 1561
BspFNI CGCG 1 cut(s) 437
BspLI GGNNCC 6 cut(s) 135, 399, 655, 853, 952, 1335
BspMI ACCTGC 1 cut(s) 814
BspPI GGATC 4 cut(s) 945, 958, 1043, 1557
BspT104I TTCGAA 1 cut(s) 1041
BspT107I GGYRCC 1 cut(s) 397
BspTNI GGTCTC 1 cut(s) 251
BsrDI GCAATG 4 cut(s) 235, 855, 906, 924
BsrI ACTGG 3 cut(s) 635, 954, 964
BssECI CCNNGG 5 cut(s) 241, 393, 460, 759, 1494
BssMI GATC 9 cut(s) 123, 443, 513, 950, 985, 1035, 1239, 1558, 1562
BssNI GRCGYC 1 cut(s) 570
BssT1I CCWWGG 1 cut(s) 241
Bst2UI CCWGG 1 cut(s) 461
Bst4CI ACNGT 5 cut(s) 26, 562, 802, 917, 935
Bst6I CTCTTC 3 cut(s) 690, 711, 1293
BstACI GRCGYC 1 cut(s) 570
BstAPI GCANNNNNTGC 1 cut(s) 1438
BstBAI YACGTR 1 cut(s) 51
BstBI TTCGAA 1 cut(s) 1041
BstC8I GCNNGC 5 cut(s) 225, 590, 834, 850, 1189
BstDEI CTNAG 1 cut(s) 741
BstDSI CCRYGG 2 cut(s) 241, 1494
BstEII GGTNACC 1 cut(s) 773
BstF5I GGATG 2 cut(s) 850, 1150
BstFNI CGCG 1 cut(s) 437
BstHHI GCGC 1 cut(s) 104
BstKTI GATC 9 cut(s) 126, 446, 516, 953, 988, 1038, 1242, 1561, 1565
BstMAI GTCTC 2 cut(s) 251, 571
BstMBI GATC 9 cut(s) 123, 443, 513, 950, 985, 1035, 1239, 1558, 1562
BstMCI CGRYCG 1 cut(s) 435
BstMWI GCNNNNNNNGC 4 cut(s) 908, 1438, 1505, 1517
BstNI CCWGG 1 cut(s) 461
BstPAI GACNNNNGTC 1 cut(s) 1374
BstPI GGTNACC 1 cut(s) 773
BstSCI CCNGG 3 cut(s) 459, 655, 860
BstSFI CTRYAG 1 cut(s) 405
BstSNI TACGTA 1 cut(s) 51
BstUI CGCG 1 cut(s) 437
BstV1I GCAGC 8 cut(s) 397, 536, 838, 886, 889, 1444, 1495, 1507
BstV2I GAAGAC 1 cut(s) 1206
BstX2I RGATCY 3 cut(s) 123, 950, 1035
BstXI CCANNNNNNTGG 1 cut(s) 676
BstYI RGATCY 3 cut(s) 123, 950, 1035
Bsu15I ATCGAT 3 cut(s) 446, 1242, 1561
BsuI GTATCC 1 cut(s) 63
BsuRI GGCC 3 cut(s) 379, 634, 836
BsuTUI ATCGAT 3 cut(s) 446, 1242, 1561
BtgI CCRYGG 2 cut(s) 241, 1494
BtgZI GCGATG 1 cut(s) 534
BtsCI GGATG 2 cut(s) 850, 1150
BtsI GCAGTG 1 cut(s) 287
BtsIMutI CAGTG 3 cut(s) 287, 628, 798
BveI ACCTGC 1 cut(s) 814
Cac8I GCNNGC 5 cut(s) 225, 590, 834, 850, 1189
CfoI GCGC 1 cut(s) 104
Cfr13I GGNCC 3 cut(s) 471, 653, 1552
ClaI ATCGAT 3 cut(s) 446, 1242, 1561
CseI GACGC 3 cut(s) 375, 402, 578
Csp6I GTAC 4 cut(s) 207, 803, 811, 957
CviAII CATG 4 cut(s) 242, 354, 622, 670
CviQI GTAC 4 cut(s) 207, 803, 811, 957
DdeI CTNAG 1 cut(s) 741
DpnI GATC 9 cut(s) 125, 445, 515, 952, 987, 1037, 1241, 1560, 1564
DpnII GATC 9 cut(s) 123, 443, 513, 950, 985, 1035, 1239, 1558, 1562
DraI TTTAAA 1 cut(s) 322
DraIII CACNNNGTG 1 cut(s) 1114
DrdI GACNNNNNNGTC 1 cut(s) 608
DseDI GACNNNNNNGTC 1 cut(s) 608
Eam1104I CTCTTC 3 cut(s) 690, 711, 1293
EarI CTCTTC 3 cut(s) 690, 711, 1293
EciI GGCGGA 1 cut(s) 728
Eco105I TACGTA 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 241
Eco24I GRGCYC 1 cut(s) 867
Eco31I GGTCTC 1 cut(s) 251
Eco47I GGWCC 3 cut(s) 471, 653, 1552
Eco91I GGTNACC 1 cut(s) 773
EcoO65I GGTNACC 1 cut(s) 773
EcoRI GAATTC 1 cut(s) 1416
EcoRII CCWGG 1 cut(s) 459
EcoT14I CCWWGG 1 cut(s) 241
EcoT22I ATGCAT 1 cut(s) 237
EcoT38I GRGCYC 1 cut(s) 867
ErhI CCWWGG 1 cut(s) 241
FaeI CATG 4 cut(s) 245, 357, 625, 673
FaqI GGGAC 2 cut(s) 639, 1012
FatI CATG 4 cut(s) 241, 353, 621, 669
FauI CCCGC 1 cut(s) 989
FblI GTMKAC 1 cut(s) 1409
FokI GGATG 2 cut(s) 857, 1157
FriOI GRGCYC 1 cut(s) 867
FspBI CTAG 1 cut(s) 1284
FspI TGCGCA 1 cut(s) 103
GlaI GCGC 1 cut(s) 103
HaeIII GGCC 3 cut(s) 379, 634, 836
HapII CCGG 3 cut(s) 210, 657, 861
HgaI GACGC 3 cut(s) 375, 402, 578
HhaI GCGC 1 cut(s) 104
Hin1I GRCGYC 1 cut(s) 570
Hin1II CATG 4 cut(s) 245, 357, 625, 673
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HincII GTYRAC 1 cut(s) 1410
HindII GTYRAC 1 cut(s) 1410
HinfI GANTC 5 cut(s) 579, 821, 1244, 1358, 1375
HpaII CCGG 3 cut(s) 210, 657, 861
HphI GGTGA 4 cut(s) 487, 767, 1073, 1091
Hpy166II GTNNAC 1 cut(s) 1410
Hpy188I TCNGA 1 cut(s) 601
Hpy188III TCNNGA 7 cut(s) 31, 121, 181, 332, 613, 938, 1566
Hpy8I GTNNAC 1 cut(s) 1410
Hpy99I CGWCG 4 cut(s) 418, 572, 1414, 1541
HpyCH4III ACNGT 5 cut(s) 26, 562, 802, 917, 935
HpyCH4IV ACGT 1 cut(s) 50
HpyCH4V TGCA 9 cut(s) 227, 235, 292, 524, 783, 1117, 1441, 1470, 1523
HpyF10VI GCNNNNNNNGC 4 cut(s) 908, 1438, 1505, 1517
HpyF3I CTNAG 1 cut(s) 741
HpySE526I ACGT 1 cut(s) 50
Hsp92I GRCGYC 1 cut(s) 570
Hsp92II CATG 4 cut(s) 245, 357, 625, 673
HspAI GCGC 1 cut(s) 102
Kzo9I GATC 9 cut(s) 123, 443, 513, 950, 985, 1035, 1239, 1558, 1562
LmnI GCTCC 2 cut(s) 505, 857
Lsp1109I GCAGC 8 cut(s) 397, 536, 838, 886, 889, 1444, 1495, 1507
LweI GCATC 3 cut(s) 395, 511, 835
MaeI CTAG 1 cut(s) 1284
MaeII ACGT 1 cut(s) 50
MaeIII GTNAC 2 cut(s) 773, 796
MalI GATC 9 cut(s) 125, 445, 515, 952, 987, 1037, 1241, 1560, 1564
MboI GATC 9 cut(s) 123, 443, 513, 950, 985, 1035, 1239, 1558, 1562
MfeI CAATTG 1 cut(s) 1023
MflI RGATCY 3 cut(s) 123, 950, 1035
MhlI GDGCHC 1 cut(s) 867
MluCI AATT 9 cut(s) 58, 185, 323, 682, 1023, 1278, 1416, 1463, 1524
MlyI GAGTC 3 cut(s) 573, 815, 1384
MmeI TCCRAC 1 cut(s) 624
Mph1103I ATGCAT 1 cut(s) 237
MseI TTAA 6 cut(s) 15, 44, 321, 1104, 1125, 1529
MslI CAYNNNNRTG 3 cut(s) 240, 674, 1074
MspA1I CMGCKG 2 cut(s) 410, 829
MspI CCGG 3 cut(s) 210, 657, 861
MspR9I CCNGG 3 cut(s) 461, 657, 862
MunI CAATTG 1 cut(s) 1023
MvaI CCWGG 1 cut(s) 461
MvnI CGCG 1 cut(s) 437
MwoI GCNNNNNNNGC 4 cut(s) 908, 1438, 1505, 1517
NciI CCSGG 2 cut(s) 657, 862
NcoI CCATGG 1 cut(s) 241
NdeII GATC 9 cut(s) 123, 443, 513, 950, 985, 1035, 1239, 1558, 1562
NlaIII CATG 4 cut(s) 245, 357, 625, 673
NlaIV GGNNCC 6 cut(s) 135, 399, 655, 853, 952, 1335
NmeAIII GCCGAG 2 cut(s) 374, 784
NmuCI GTSAC 2 cut(s) 773, 796
NsbI TGCGCA 1 cut(s) 103
NsiI ATGCAT 1 cut(s) 237
NspV TTCGAA 1 cut(s) 1041
PcsI WCGNNNNNNNCGW 2 cut(s) 47, 495
PfeI GAWTC 2 cut(s) 1244, 1358
PflFI GACNNNGTC 1 cut(s) 1210
PleI GAGTC 3 cut(s) 573, 815, 1383
PpsI GAGTC 3 cut(s) 573, 815, 1383
Ppu21I YACGTR 1 cut(s) 51
PshAI GACNNNNGTC 1 cut(s) 1374
Psp6I CCWGG 1 cut(s) 459
PspEI GGTNACC 1 cut(s) 773
PspGI CCWGG 1 cut(s) 459
PspN4I GGNNCC 6 cut(s) 135, 399, 655, 853, 952, 1335
PspPI GGNCC 3 cut(s) 471, 653, 1552
PsuI RGATCY 3 cut(s) 123, 950, 1035
PsyI GACNNNGTC 1 cut(s) 1210
PvuII CAGCTG 1 cut(s) 410
RsaI GTAC 4 cut(s) 208, 804, 812, 958
RsaNI GTAC 4 cut(s) 207, 803, 811, 957
RseI CAYNNNNRTG 3 cut(s) 240, 674, 1074
SalI GTCGAC 1 cut(s) 1408
SaqAI TTAA 6 cut(s) 15, 44, 321, 1104, 1125, 1529
Sau3AI GATC 9 cut(s) 123, 443, 513, 950, 985, 1035, 1239, 1558, 1562
Sau96I GGNCC 3 cut(s) 471, 653, 1552
ScaI AGTACT 1 cut(s) 958
SchI GAGTC 3 cut(s) 573, 815, 1384
ScrFI CCNGG 3 cut(s) 461, 657, 862
SduI GDGCHC 1 cut(s) 867
SfaNI GCATC 3 cut(s) 395, 511, 835
SfcI CTRYAG 1 cut(s) 405
SfuI TTCGAA 1 cut(s) 1041
SinI GGWCC 3 cut(s) 471, 653, 1552
SmiMI CAYNNNNRTG 3 cut(s) 240, 674, 1074
SnaBI TACGTA 1 cut(s) 51
Sse9I AATT 9 cut(s) 58, 185, 323, 682, 1023, 1278, 1416, 1463, 1524
SsiI CCGC 8 cut(s) 435, 592, 710, 713, 829, 996, 1221, 1533
SspMI CTAG 1 cut(s) 1284
StyD4I CCNGG 3 cut(s) 459, 655, 860
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 5 cut(s) 26, 562, 802, 917, 935
TaiI ACGT 1 cut(s) 53
TasI AATT 9 cut(s) 58, 185, 323, 682, 1023, 1278, 1416, 1463, 1524
TatI WGTACW 1 cut(s) 956
TauI GCSGC 3 cut(s) 713, 832, 1536
TfiI GAWTC 2 cut(s) 1244, 1358
Tru1I TTAA 6 cut(s) 15, 44, 321, 1104, 1125, 1529
Tru9I TTAA 6 cut(s) 15, 44, 321, 1104, 1125, 1529
TscAI CASTG 3 cut(s) 294, 635, 805
TseFI GTSAC 2 cut(s) 773, 796
TseI GCWGC 8 cut(s) 410, 524, 826, 899, 902, 1432, 1508, 1520
Tsp45I GTSAC 2 cut(s) 773, 796
TspDTI ATGAA 4 cut(s) 342, 560, 638, 1319
TspGWI ACGGA 2 cut(s) 518, 1554
TspRI CASTG 3 cut(s) 294, 635, 805
Tth111I GACNNNGTC 1 cut(s) 1210
VpaK11BI GGWCC 3 cut(s) 471, 653, 1552
XapI RAATTY 3 cut(s) 185, 323, 1416
XmiI GTMKAC 1 cut(s) 1409
XspI CTAG 1 cut(s) 1284
ZrmI AGTACT 1 cut(s) 958
Zsp2I ATGCAT 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.