pycom17g26150

Uncharacterized protein At4g22758-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0022415
Physical Location & Seq
Reverse (-)
24463435 .. 24464268
834 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 459 bp
ATGCTTAAGAGGAAGGTTTTGGAGAGAATTAATTTACAGAAGCATAAGAAGACCAGTACTGATGCTGCTCATACTGTGAAGACGAACAGGTTTTTGATCACAGTTAATGTTCTTGGAAGCGCAGGACCCATAAGGTTTGTGGTGAACGATGATGATCTCGTAGCTCGAGTCATCGACACTGCTCTTAAATCCTACGCCCGCGAAGGCCGGCTACCTGTTCTTGGTTCAGATGTCAAGGATTTCCTTCTACACTGTGCTAATGCAGGATCTGACGCTTTAAGTCCGTGGGAAACTATAGGATCTCGTGGGGGAAGGAACTTTGTGCTGTCCAAGAAGGAGAAGCAAGTGCAGAATAATATTGCTGAAAGAAAGTCGGAGATAACAGCTCCAAAGGGAACGGTGAGTAGCTGGAAGGCATGGTTTAACAAATCATTCACTTTCAAGATATCATCTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

16.92

Weight (kDa)

10.15

Isoelectric Point (pI)

28.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 27 - 109 2.8e-30 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022415)

Species Orthologous Gene IDs
malus_domestica MD17G1260800.v1.1
prunus_persica Prupe.3G162600_v2.0.a1
pyrus_communis pycom09g17850 pycom17g26150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 201
AciI CCGC 1 cut(s) 199
AclWI GGATC 2 cut(s) 274, 307
AfaI GTAC 1 cut(s) 58
AfiI CCNNNNNNNGG 1 cut(s) 221
AflII CTTAAG 1 cut(s) 5
AgsI TTSAA 1 cut(s) 442
AluBI AGCT 3 cut(s) 164, 386, 408
AluI AGCT 3 cut(s) 164, 386, 408
AlwI GGATC 2 cut(s) 274, 307
AlwNI CAGNNNCTG 1 cut(s) 269
Ama87I CYCGRG 1 cut(s) 165
AoxI GGCC 1 cut(s) 205
ApeKI GCWGC 1 cut(s) 65
AseI ATTAAT 1 cut(s) 30
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 125
AsuHPI GGTGA 2 cut(s) 154, 412
AvaI CYCGRG 1 cut(s) 165
AvaII GGWCC 1 cut(s) 125
BarI GAAGNNNNNNTAC 2 cut(s) 195, 227
BauI CACGAG 1 cut(s) 303
BbsI GAAGAC 2 cut(s) 56, 86
BbvI GCAGC 1 cut(s) 52
BclI TGATCA 1 cut(s) 96
BfmI CTRYAG 1 cut(s) 294
BfrI CTTAAG 1 cut(s) 5
BisI GCNGC 1 cut(s) 66
BlsI GCNGC 1 cut(s) 67
BmcAI AGTACT 1 cut(s) 58
Bme18I GGWCC 1 cut(s) 125
BmeT110I CYCGRG 1 cut(s) 165
BmgT120I GGNCC 1 cut(s) 125
BmiI GGNNCC 1 cut(s) 127
BmsI GCATC 1 cut(s) 52
BpiI GAAGAC 2 cut(s) 56, 86
BsaBI GATNNNNATC 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 284
Bsc4I CCNNNNNNNGG 1 cut(s) 221
Bse118I RCCGGY 1 cut(s) 207
Bse1I ACTGG 1 cut(s) 54
Bse8I GATNNNNATC 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 284
BseJI GATNNNNATC 1 cut(s) 153
BseLI CCNNNNNNNGG 1 cut(s) 221
BseNI ACTGG 1 cut(s) 54
BseXI GCAGC 1 cut(s) 52
BsgI GTGCAG 1 cut(s) 368
Bsh1236I CGCG 1 cut(s) 201
BshFI GGCC 1 cut(s) 207
BsiHKCI CYCGRG 1 cut(s) 165
BsiSI CCGG 1 cut(s) 208
BslI CCNNNNNNNGG 1 cut(s) 221
BsnI GGCC 1 cut(s) 207
BsoBI CYCGRG 1 cut(s) 165
Bsp143I GATC 4 cut(s) 96, 154, 266, 299
BspACI CCGC 1 cut(s) 199
BspANI GGCC 1 cut(s) 207
BspFNI CGCG 1 cut(s) 201
BspLI GGNNCC 1 cut(s) 127
BspPI GGATC 2 cut(s) 274, 307
BspTI CTTAAG 1 cut(s) 5
BsrFI RCCGGY 1 cut(s) 207
BsrI ACTGG 1 cut(s) 54
BssAI RCCGGY 1 cut(s) 207
BssECI CCNNGG 1 cut(s) 284
BssMI GATC 4 cut(s) 96, 154, 266, 299
BssSI CACGAG 1 cut(s) 303
Bst2BI CACGAG 1 cut(s) 303
Bst4CI ACNGT 4 cut(s) 76, 103, 254, 400
BstAFI CTTAAG 1 cut(s) 5
BstC8I GCNNGC 2 cut(s) 199, 209
BstDSI CCRYGG 1 cut(s) 284
BstFNI CGCG 1 cut(s) 201
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 4 cut(s) 99, 157, 269, 302
BstMBI GATC 4 cut(s) 96, 154, 266, 299
BstSFI CTRYAG 1 cut(s) 294
BstUI CGCG 1 cut(s) 201
BstV1I GCAGC 1 cut(s) 52
BstV2I GAAGAC 2 cut(s) 56, 86
BstX2I RGATCY 2 cut(s) 266, 299
BstYI RGATCY 2 cut(s) 266, 299
BsuRI GGCC 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 284
BtsI GCAGTG 1 cut(s) 177
BtsIMutI CAGTG 2 cut(s) 177, 250
Cac8I GCNNGC 2 cut(s) 199, 209
CaiI CAGNNNCTG 1 cut(s) 269
CfoI GCGC 1 cut(s) 122
Cfr10I RCCGGY 1 cut(s) 207
Cfr13I GGNCC 1 cut(s) 125
CseI GACGC 1 cut(s) 281
Csp6I GTAC 1 cut(s) 57
CviAII CATG 1 cut(s) 417
CviJI RGCY 5 cut(s) 164, 207, 211, 386, 408
CviKI_1 RGCY 5 cut(s) 164, 207, 211, 386, 408
CviQI GTAC 1 cut(s) 57
DpnI GATC 4 cut(s) 98, 156, 268, 301
DpnII GATC 4 cut(s) 96, 154, 266, 299
Eco32I GATATC 1 cut(s) 447
Eco47I GGWCC 1 cut(s) 125
Eco88I CYCGRG 1 cut(s) 165
EcoO109I RGGNCCY 1 cut(s) 125
EcoRV GATATC 1 cut(s) 447
FaeI CATG 1 cut(s) 420
FaiI YATR 5 cut(s) 45, 72, 131, 296, 418
FatI CATG 1 cut(s) 416
FauI CCCGC 1 cut(s) 206
FbaI TGATCA 1 cut(s) 96
Fnu4HI GCNGC 1 cut(s) 66
Fsp4HI GCNGC 1 cut(s) 66
GlaI GCGC 1 cut(s) 121
GluI GCNGC 1 cut(s) 66
HaeIII GGCC 1 cut(s) 207
HapII CCGG 1 cut(s) 208
HgaI GACGC 1 cut(s) 281
HhaI GCGC 1 cut(s) 122
Hin1II CATG 1 cut(s) 420
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HinfI GANTC 1 cut(s) 168
HpaII CCGG 1 cut(s) 208
HphI GGTGA 2 cut(s) 154, 412
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 3 cut(s) 229, 271, 376
Hpy188III TCNNGA 1 cut(s) 442
Hpy8I GTNNAC 1 cut(s) 145
HpyAV CCTTC 6 cut(s) 7, 197, 254, 306, 328, 406
HpyCH4III ACNGT 4 cut(s) 76, 103, 254, 400
HpyCH4V TGCA 2 cut(s) 263, 349
Hsp92II CATG 1 cut(s) 420
HspAI GCGC 1 cut(s) 120
KroI GCCGGC 1 cut(s) 207
KroNI GCCGGC 1 cut(s) 209
Ksp22I TGATCA 1 cut(s) 96
Kzo9I GATC 4 cut(s) 96, 154, 266, 299
LmnI GCTCC 1 cut(s) 391
LpnPI CCDG 7 cut(s) 67, 73, 108, 221, 228, 249, 394
Lsp1109I GCAGC 1 cut(s) 52
LweI GCATC 1 cut(s) 52
MalI GATC 4 cut(s) 98, 156, 268, 301
MboI GATC 4 cut(s) 96, 154, 266, 299
MboII GAAGA 2 cut(s) 61, 91
MflI RGATCY 2 cut(s) 266, 299
MluCI AATT 2 cut(s) 27, 31
MlyI GAGTC 1 cut(s) 177
MmeI TCCRAC 1 cut(s) 354
MnlI CCTC 1 cut(s) 3
MroNI GCCGGC 1 cut(s) 207
MseI TTAA 6 cut(s) 6, 30, 105, 186, 278, 423
MspCI CTTAAG 1 cut(s) 5
MspI CCGG 1 cut(s) 208
MvnI CGCG 1 cut(s) 201
NaeI GCCGGC 1 cut(s) 209
NdeII GATC 4 cut(s) 96, 154, 266, 299
NgoMIV GCCGGC 1 cut(s) 207
NlaIII CATG 1 cut(s) 420
NlaIV GGNNCC 1 cut(s) 127
PaeR7I CTCGAG 1 cut(s) 165
PdiI GCCGGC 1 cut(s) 209
PkrI GCNGC 1 cut(s) 67
PleI GAGTC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 176
PpuMI RGGWCCY 1 cut(s) 125
PshBI ATTAAT 1 cut(s) 30
Psp5II RGGWCCY 1 cut(s) 125
PspN4I GGNNCC 1 cut(s) 127
PspPI GGNCC 1 cut(s) 125
PspPPI RGGWCCY 1 cut(s) 125
PspXI VCTCGAGB 1 cut(s) 165
PstNI CAGNNNCTG 1 cut(s) 269
PsuI RGATCY 2 cut(s) 266, 299
RsaI GTAC 1 cut(s) 58
RsaNI GTAC 1 cut(s) 57
SaqAI TTAA 6 cut(s) 6, 30, 105, 186, 278, 423
SatI GCNGC 1 cut(s) 66
Sau3AI GATC 4 cut(s) 96, 154, 266, 299
Sau96I GGNCC 1 cut(s) 125
ScaI AGTACT 1 cut(s) 58
SchI GAGTC 1 cut(s) 177
SetI ASST 7 cut(s) 18, 92, 137, 166, 217, 388, 410
SfaNI GCATC 1 cut(s) 52
SfcI CTRYAG 1 cut(s) 294
Sfr274I CTCGAG 1 cut(s) 165
SinI GGWCC 1 cut(s) 125
SlaI CTCGAG 1 cut(s) 165
SmlI CTYRAG 2 cut(s) 5, 165
SmoI CTYRAG 2 cut(s) 5, 165
Sse9I AATT 2 cut(s) 27, 31
SsiI CCGC 1 cut(s) 199
SspI AATATT 1 cut(s) 358
TaaI ACNGT 4 cut(s) 76, 103, 254, 400
TaqI TCGA 2 cut(s) 166, 174
TasI AATT 2 cut(s) 27, 31
TatI WGTACW 1 cut(s) 56
Tru1I TTAA 6 cut(s) 6, 30, 105, 186, 278, 423
Tru9I TTAA 6 cut(s) 6, 30, 105, 186, 278, 423
TscAI CASTG 2 cut(s) 184, 257
TseI GCWGC 1 cut(s) 65
TspGWI ACGGA 1 cut(s) 273
TspRI CASTG 2 cut(s) 184, 257
Vha464I CTTAAG 1 cut(s) 5
VpaK11BI GGWCC 1 cut(s) 125
VspI ATTAAT 1 cut(s) 30
XcmI CCANNNNNNNNNTGG 1 cut(s) 136
XhoI CTCGAG 1 cut(s) 165
ZrmI AGTACT 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.