pycom17g26540

phospholipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0012940
Physical Location & Seq
Forward (+)
24828516 .. 24830017
1502 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1383 bp
ATGGGCAACAATAATGCTAAACATACAAAAACAACAAGCCATCCTCTCCCTCCGACGTCCATGGCCACCAACCAAACTGCTGATCATCCAGCAGCCGCTGCATGGTCAGAGCTTCTAGGCTCAAACAACTGGGATGGCCTCATGGACCCTCTAGACCTCAATCTCCGAACCCTCATCCTCCGCTGCGGCAACTTCTGCCAGGCCACCTACGACGCCTTCAACAACGACCAAAACTCCAAGTACTGCGGATCCAGCCGCTATGGCAAACACTCCTTCTTCGACAAGGTCATGCTCCAAGACGCCGCCAACTACCAAGTCTCTTGCTTCCTCTACGCCACAGCCCAAGTCAGCGTCCCCGAAGCCTTGCTTCTCAAATCCAAGTCCCGCGAGTCATGGGATCGGGAGTCTAACTGGATCGGCTACATCGCCGTCACCACCGATGAAGTCAGCAAAGCCATCGGTCGACGAGAAATATACGTCGCATGGCGCGGCACGATTAGAAACTACGAGTGGATTAATGTGATGGGAGCTGAGCTAGAGTCCGCGGAGATTATTTTAGGTTCTTCTCATGCCAGCCTAGCGAATGGTCATCAAAATCACGATGACAAGGATGACGATGAAGATGACGAAAAAGTGCCGAAAGTCATGAGGGGTTGGCTTACAATGTATATTTCCAACGACCCGAAGTCCCCGTTTACGAAAACAAGCGCCAGAGTCCAACTTGTGACAAAAATCAACGAGCTGAGGAACAAGTACAAGGACGAGAAGCTGAGCATAACCATGACAGGACACAGTCTCGGCGCGAGTTTAGCGGTTTTGAGCGCTTTCGATTTGGCCGAAAACGGGGTTTCCGATCTCCCAATTGCGGTTTTTGTATTTGGTTGTCCACAAATCGGAAACAGTGCACTGAAAAGACGGATTGAGTGGCACCCGAATCTGAAGATACTGCATATTAAGAACACCATCGATGTAATTCCGCACTATCCTGTCCGTGTAATGGGGTACCGGAAGGTCGGGACGGTGCTTTTGATTGATACGAGGAAGTCGCCGTTCTTGAAGGAGTCGAAGAATCCGAGCGACTGGCATAACTTGGAGGGGATTCTGCATGTGGTGGCGGGGTGGAAAGGGGAGGACGCAGAGTTTGAGCTGAGGGTGAAGAGGAGCGTGGCGTTGGTGAACAAGTCATGTGATTTTCTCAAGGAGGAGTGTAATGTGCCCGCGTTGTGGTGGGTGGAGAAGAACAGAGAAGTGGTGGGTTCCGCCTTCGTAAGGTCAAGTCAATGGTTCCATATACAGTACTGTAATGTTGTTTTGTCTTGTTTAAGTTGTTTGTGTCGGTCTCTTTTGTTTAATGTTTGTCATGTGCAGCTCTATTTTGTTTAA

Protein Analysis

461

Amino Acids

51.92

Weight (kDa)

7.22

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 159 - 331 9.5e-27 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012940)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G42690
fragaria_vesca FvH4_6g27580
malus_domestica MD00G1191600.v1.1 MD11G1004600.v1.1 MD17G1265700.v1.1
prunus_persica Prupe.3G116300_v2.0.a1
pyrus_communis pycom09g15480 pycom17g26540
rosa_laevigata RLG00000019119
rosa_multiflora Rmu_sc0005625.1_g000015 Rmu_sc0009900.1_g000007
rosa_roxburghii Rroxscaffold_2G00114140
rosa_rugosa Rorug02G0291000
rosa_samantha Rh2AG342400 Rh2BG350300 Rh2CG329500 Rh2DG368500
rosa_wichuraiana Rw2G027700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 59
Acc65I GGTACC 1 cut(s) 1002
AccB1I GGYRCC 2 cut(s) 927, 1002
AccI GTMKAC 1 cut(s) 463
AccII CGCG 5 cut(s) 387, 489, 545, 803, 1220
AclWI GGATC 4 cut(s) 243, 256, 405, 422
AcoI YGGCCR 2 cut(s) 63, 834
AcuI CTGAAG 1 cut(s) 959
AcyI GRCGYC 3 cut(s) 56, 213, 300
AfaI GTAC 4 cut(s) 242, 755, 1004, 1298
AfeI AGCGCT 1 cut(s) 823
AfiI CCNNNNNNNGG 7 cut(s) 102, 843, 865, 893, 997, 1224, 1269
AgsI TTSAA 2 cut(s) 220, 1057
AhdI GACNNNNNGTC 1 cut(s) 685
AjnI CCWGG 1 cut(s) 198
AluBI AGCT 7 cut(s) 112, 530, 535, 742, 769, 1147, 1369
AluI AGCT 7 cut(s) 112, 530, 535, 742, 769, 1147, 1369
Alw21I GWGCWC 1 cut(s) 907
Alw26I GTCTC 3 cut(s) 322, 800, 1344
Alw44I GTGCAC 1 cut(s) 903
AlwI GGATC 4 cut(s) 243, 256, 405, 422
AlwNI CAGNNNCTG 1 cut(s) 98
Aor51HI AGCGCT 1 cut(s) 823
AoxI GGCC 4 cut(s) 63, 136, 201, 834
ApaLI GTGCAC 1 cut(s) 903
ApeKI GCWGC 4 cut(s) 92, 98, 183, 1366
ArsI GACNNNNNNTTYG 4 cut(s) 1009, 1041, 1124, 1156
AseI ATTAAT 1 cut(s) 516
Asp718I GGTACC 1 cut(s) 1002
AspLEI GCGC 4 cut(s) 489, 710, 803, 824
AspS9I GGNCC 1 cut(s) 145
AsuHPI GGTGA 3 cut(s) 424, 1165, 1186
AvaII GGWCC 1 cut(s) 145
BaeGI GKGCMC 2 cut(s) 907, 1218
BalI TGGCCA 1 cut(s) 65
BamHI GGATCC 1 cut(s) 248
BanI GGYRCC 2 cut(s) 927, 1002
Bbv12I GWGCWC 1 cut(s) 907
BbvCI CCTCAGC 2 cut(s) 743, 1148
BbvI GCAGC 4 cut(s) 85, 104, 170, 1378
BccI CCATC 5 cut(s) 48, 128, 464, 517, 971
BceAI ACGGC 2 cut(s) 413, 1033
BcgI CGANNNNNNTGC 2 cut(s) 439, 473
BciT130I CCWGG 1 cut(s) 200
BclI TGATCA 1 cut(s) 82
BcoDI GTCTC 3 cut(s) 322, 800, 1344
BfaI CTAG 4 cut(s) 116, 152, 536, 578
BfoI RGCGCY 2 cut(s) 711, 825
BglI GCCNNNNNGGC 1 cut(s) 261
BisI GCNGC 9 cut(s) 93, 96, 99, 184, 187, 256, 303, 490, 1367
BlpI GCTNAGC 2 cut(s) 531, 770
BlsI GCNGC 9 cut(s) 94, 97, 100, 185, 188, 257, 304, 491, 1368
BmcAI AGTACT 2 cut(s) 242, 1298
Bme1390I CCNGG 1 cut(s) 200
Bme18I GGWCC 1 cut(s) 145
BmeRI GACNNNNNGTC 1 cut(s) 685
BmgT120I GGNCC 1 cut(s) 145
BmiI GGNNCC 6 cut(s) 147, 250, 929, 1004, 1258, 1286
BmrFI CCNGG 1 cut(s) 200
BmrI ACTGGG 1 cut(s) 139
BmuI ACTGGG 1 cut(s) 139
Bpu10I CCTNAGC 2 cut(s) 743, 1148
Bpu1102I GCTNAGC 2 cut(s) 531, 770
BpuEI CTTGAG 1 cut(s) 1181
Bsa29I ATCGAT 1 cut(s) 966
BsaHI GRCGYC 3 cut(s) 56, 213, 300
BsaI GGTCTC 1 cut(s) 1344
BsaJI CCNNGG 2 cut(s) 60, 543
BsaWI WCCGGW 1 cut(s) 1005
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 7 cut(s) 102, 843, 865, 893, 997, 1224, 1269
Bse1I ACTGG 3 cut(s) 134, 416, 1085
BseBI CCWGG 1 cut(s) 200
BseCI ATCGAT 1 cut(s) 966
BseDI CCNNGG 2 cut(s) 60, 543
BseGI GGATG 5 cut(s) 40, 85, 139, 174, 616
BseLI CCNNNNNNNGG 7 cut(s) 102, 843, 865, 893, 997, 1224, 1269
BseMII CTCAG 4 cut(s) 522, 734, 761, 1139
BseNI ACTGG 3 cut(s) 134, 416, 1085
BseRI GAGGAG 2 cut(s) 1174, 1217
BseSI GKGCMC 2 cut(s) 907, 1218
BseXI GCAGC 4 cut(s) 85, 104, 170, 1378
Bsh1236I CGCG 5 cut(s) 387, 489, 545, 803, 1220
Bsh1285I CGRYCG 1 cut(s) 463
BshFI GGCC 4 cut(s) 65, 138, 203, 836
BshNI GGYRCC 2 cut(s) 927, 1002
BshVI ATCGAT 1 cut(s) 966
BsiEI CGRYCG 1 cut(s) 463
BsiHKAI GWGCWC 1 cut(s) 907
BsiSI CCGG 1 cut(s) 1006
BslFI GGGAC 4 cut(s) 338, 367, 673, 1030
BslI CCNNNNNNNGG 7 cut(s) 102, 843, 865, 893, 997, 1224, 1269
BsmAI GTCTC 3 cut(s) 322, 800, 1344
BsmFI GGGAC 4 cut(s) 338, 367, 673, 1030
BsnI GGCC 4 cut(s) 65, 138, 203, 836
Bso31I GGTCTC 1 cut(s) 1344
Bsp1286I GDGCHC 2 cut(s) 907, 1218
Bsp143I GATC 5 cut(s) 82, 248, 397, 414, 853
Bsp1720I GCTNAGC 2 cut(s) 531, 770
Bsp19I CCATGG 1 cut(s) 60
BspANI GGCC 4 cut(s) 65, 138, 203, 836
BspCNI CTCAG 4 cut(s) 523, 735, 762, 1140
BspDI ATCGAT 1 cut(s) 966
BspFNI CGCG 5 cut(s) 387, 489, 545, 803, 1220
BspHI TCATGA 1 cut(s) 645
BspLI GGNNCC 6 cut(s) 147, 250, 929, 1004, 1258, 1286
BspPI GGATC 4 cut(s) 243, 256, 405, 422
BspT107I GGYRCC 2 cut(s) 927, 1002
BspTNI GGTCTC 1 cut(s) 1344
BsrI ACTGG 3 cut(s) 134, 416, 1085
BssECI CCNNGG 2 cut(s) 60, 543
BssMI GATC 5 cut(s) 82, 248, 397, 414, 853
BssNI GRCGYC 3 cut(s) 56, 213, 300
BssT1I CCWWGG 1 cut(s) 60
Bst2UI CCWGG 1 cut(s) 200
Bst4CI ACNGT 5 cut(s) 794, 902, 1021, 1296, 1301
Bst6I CTCTTC 1 cut(s) 1151
BstACI GRCGYC 3 cut(s) 56, 213, 300
BstAPI GCANNNNNTGC 2 cut(s) 98, 195
BstC8I GCNNGC 2 cut(s) 574, 1218
BstDEI CTNAG 4 cut(s) 531, 743, 770, 1148
BstDSI CCRYGG 2 cut(s) 60, 543
BstENI CCTNNNNNAGG 1 cut(s) 1267
BstF5I GGATG 5 cut(s) 40, 85, 139, 174, 616
BstFNI CGCG 5 cut(s) 387, 489, 545, 803, 1220
BstH2I RGCGCY 2 cut(s) 711, 825
BstHHI GCGC 4 cut(s) 489, 710, 803, 824
BstKTI GATC 5 cut(s) 85, 251, 400, 417, 856
BstMAI GTCTC 3 cut(s) 322, 800, 1344
BstMBI GATC 5 cut(s) 82, 248, 397, 414, 853
BstMCI CGRYCG 1 cut(s) 463
BstMWI GCNNNNNNNGC 6 cut(s) 98, 195, 252, 261, 578, 809
BstNI CCWGG 1 cut(s) 200
BstNSI RCATGY 1 cut(s) 1109
BstSCI CCNGG 1 cut(s) 198
BstSLI GKGCMC 2 cut(s) 907, 1218
BstUI CGCG 5 cut(s) 387, 489, 545, 803, 1220
BstV1I GCAGC 4 cut(s) 85, 104, 170, 1378
BstX2I RGATCY 1 cut(s) 248
BstYI RGATCY 1 cut(s) 248
Bsu15I ATCGAT 1 cut(s) 966
BsuRI GGCC 4 cut(s) 65, 138, 203, 836
BsuTUI ATCGAT 1 cut(s) 966
BtgI CCRYGG 2 cut(s) 60, 543
BtgZI GCGATG 1 cut(s) 409
BtsCI GGATG 5 cut(s) 40, 85, 139, 174, 616
BtsIMutI CAGTG 2 cut(s) 905, 907
Cac8I GCNNGC 2 cut(s) 574, 1218
CaiI CAGNNNCTG 1 cut(s) 98
CciI TCATGA 1 cut(s) 645
CfoI GCGC 4 cut(s) 489, 710, 803, 824
Cfr13I GGNCC 1 cut(s) 145
Cfr42I CCGCGG 1 cut(s) 546
ClaI ATCGAT 1 cut(s) 966
CseI GACGC 4 cut(s) 221, 308, 340, 1142
Csp6I GTAC 4 cut(s) 241, 754, 1003, 1297
CviQI GTAC 4 cut(s) 241, 754, 1003, 1297
DdeI CTNAG 4 cut(s) 531, 743, 770, 1148
DpnI GATC 5 cut(s) 84, 250, 399, 416, 855
DpnII GATC 5 cut(s) 82, 248, 397, 414, 853
DriI GACNNNNNGTC 1 cut(s) 685
EaeI YGGCCR 2 cut(s) 63, 834
Eam1104I CTCTTC 1 cut(s) 1151
Eam1105I GACNNNNNGTC 1 cut(s) 685
EarI CTCTTC 1 cut(s) 1151
EciI GGCGGA 1 cut(s) 1249
Eco130I CCWWGG 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 1344
Eco47I GGWCC 1 cut(s) 145
Eco47III AGCGCT 1 cut(s) 823
Eco57I CTGAAG 1 cut(s) 959
EcoNI CCTNNNNNAGG 1 cut(s) 1267
EcoRII CCWGG 1 cut(s) 198
EcoT14I CCWWGG 1 cut(s) 60
ErhI CCWWGG 1 cut(s) 60
FalI AAGNNNNNCTT 2 cut(s) 351, 383
FaqI GGGAC 4 cut(s) 338, 367, 673, 1030
FauI CCCGC 3 cut(s) 392, 1108, 1225
FbaI TGATCA 1 cut(s) 82
FblI GTMKAC 1 cut(s) 463
Fnu4HI GCNGC 9 cut(s) 93, 96, 99, 184, 187, 256, 303, 490, 1367
FokI GGATG 5 cut(s) 27, 72, 146, 161, 623
Fsp4HI GCNGC 9 cut(s) 93, 96, 99, 184, 187, 256, 303, 490, 1367
FspBI CTAG 4 cut(s) 116, 152, 536, 578
GlaI GCGC 4 cut(s) 488, 709, 802, 823
GluI GCNGC 9 cut(s) 93, 96, 99, 184, 187, 256, 303, 490, 1367
HaeII RGCGCY 2 cut(s) 711, 825
HaeIII GGCC 4 cut(s) 65, 138, 203, 836
HapII CCGG 1 cut(s) 1006
HgaI GACGC 4 cut(s) 221, 308, 340, 1142
HhaI GCGC 4 cut(s) 489, 710, 803, 824
Hin1I GRCGYC 3 cut(s) 56, 213, 300
Hin6I GCGC 4 cut(s) 487, 708, 801, 822
HinP1I GCGC 4 cut(s) 487, 708, 801, 822
HincII GTYRAC 1 cut(s) 464
HindII GTYRAC 1 cut(s) 464
HinfI GANTC 8 cut(s) 389, 404, 539, 714, 934, 1061, 1069, 1099
HpaII CCGG 1 cut(s) 1006
HphI GGTGA 3 cut(s) 424, 1165, 1186
Hpy166II GTNNAC 5 cut(s) 464, 696, 887, 905, 1177
Hpy188I TCNGA 7 cut(s) 54, 109, 167, 853, 896, 939, 1074
Hpy188III TCNNGA 6 cut(s) 152, 401, 599, 646, 1015, 1054
Hpy8I GTNNAC 5 cut(s) 464, 696, 887, 905, 1177
Hpy99I CGWCG 4 cut(s) 58, 215, 468, 482
HpyAV CCTTC 5 cut(s) 226, 283, 1003, 1051, 1273
HpyCH4III ACNGT 5 cut(s) 794, 902, 1021, 1296, 1301
HpyCH4IV ACGT 2 cut(s) 56, 477
HpyCH4V TGCA 5 cut(s) 101, 905, 949, 1105, 1366
HpyF10VI GCNNNNNNNGC 6 cut(s) 98, 195, 252, 261, 578, 809
HpyF3I CTNAG 4 cut(s) 531, 743, 770, 1148
HpySE526I ACGT 2 cut(s) 56, 477
Hsp92I GRCGYC 3 cut(s) 56, 213, 300
HspAI GCGC 4 cut(s) 487, 708, 801, 822
KpnI GGTACC 1 cut(s) 1006
Ksp22I TGATCA 1 cut(s) 82
KspI CCGCGG 1 cut(s) 546
Kzo9I GATC 5 cut(s) 82, 248, 397, 414, 853
LmnI GCTCC 3 cut(s) 297, 527, 1161
Lsp1109I GCAGC 4 cut(s) 85, 104, 170, 1378
MaeI CTAG 4 cut(s) 116, 152, 536, 578
MaeII ACGT 2 cut(s) 56, 477
MaeIII GTNAC 2 cut(s) 430, 724
MalI GATC 5 cut(s) 84, 250, 399, 416, 855
MboI GATC 5 cut(s) 82, 248, 397, 414, 853
MboII GAAGA 7 cut(s) 268, 555, 632, 952, 1078, 1168, 1249
MfeI CAATTG 1 cut(s) 861
MflI RGATCY 1 cut(s) 248
MhlI GDGCHC 2 cut(s) 907, 1218
MlsI TGGCCA 1 cut(s) 65
MluCI AATT 2 cut(s) 861, 972
MluNI TGGCCA 1 cut(s) 65
MlyI GAGTC 5 cut(s) 398, 413, 548, 723, 1070
MmeI TCCRAC 3 cut(s) 77, 699, 742
Mox20I TGGCCA 1 cut(s) 65
MscI TGGCCA 1 cut(s) 65
MseI TTAA 5 cut(s) 516, 954, 1322, 1350, 1381
MslI CAYNNNNRTG 1 cut(s) 779
Msp20I TGGCCA 1 cut(s) 65
MspA1I CMGCKG 3 cut(s) 98, 183, 545
MspI CCGG 1 cut(s) 1006
MspR9I CCNGG 1 cut(s) 200
MunI CAATTG 1 cut(s) 861
MvaI CCWGG 1 cut(s) 200
MvnI CGCG 5 cut(s) 387, 489, 545, 803, 1220
MwoI GCNNNNNNNGC 6 cut(s) 98, 195, 252, 261, 578, 809
NcoI CCATGG 1 cut(s) 60
NdeII GATC 5 cut(s) 82, 248, 397, 414, 853
NlaIV GGNNCC 6 cut(s) 147, 250, 929, 1004, 1258, 1286
NmeAIII GCCGAG 1 cut(s) 777
NmuCI GTSAC 2 cut(s) 430, 724
NspI RCATGY 1 cut(s) 1109
PagI TCATGA 1 cut(s) 645
PcsI WCGNNNNNNNCGW 3 cut(s) 834, 849, 1070
PfeI GAWTC 3 cut(s) 934, 1069, 1099
PflFI GACNNNGTC 2 cut(s) 284, 792
PkrI GCNGC 9 cut(s) 94, 97, 100, 185, 188, 257, 304, 491, 1368
PleI GAGTC 5 cut(s) 397, 412, 547, 722, 1069
PpsI GAGTC 5 cut(s) 397, 412, 547, 722, 1069
PshBI ATTAAT 1 cut(s) 516
Psp6I CCWGG 1 cut(s) 198
PspGI CCWGG 1 cut(s) 198
PspN4I GGNNCC 6 cut(s) 147, 250, 929, 1004, 1258, 1286
PspPI GGNCC 1 cut(s) 145
PstNI CAGNNNCTG 1 cut(s) 98
PsuI RGATCY 1 cut(s) 248
PsyI GACNNNGTC 2 cut(s) 284, 792
RsaI GTAC 4 cut(s) 242, 755, 1004, 1298
RsaNI GTAC 4 cut(s) 241, 754, 1003, 1297
RseI CAYNNNNRTG 1 cut(s) 779
SacII CCGCGG 1 cut(s) 546
SalI GTCGAC 1 cut(s) 462
SaqAI TTAA 5 cut(s) 516, 954, 1322, 1350, 1381
SatI GCNGC 9 cut(s) 93, 96, 99, 184, 187, 256, 303, 490, 1367
Sau3AI GATC 5 cut(s) 82, 248, 397, 414, 853
Sau96I GGNCC 1 cut(s) 145
ScaI AGTACT 2 cut(s) 242, 1298
SchI GAGTC 5 cut(s) 398, 413, 548, 723, 1070
ScrFI CCNGG 1 cut(s) 200
SduI GDGCHC 2 cut(s) 907, 1218
Sfr303I CCGCGG 1 cut(s) 546
SgrBI CCGCGG 1 cut(s) 546
SinI GGWCC 1 cut(s) 145
SmiMI CAYNNNNRTG 1 cut(s) 779
SmlI CTYRAG 1 cut(s) 1196
SmoI CTYRAG 1 cut(s) 1196
Sse9I AATT 2 cut(s) 861, 972
SspMI CTAG 4 cut(s) 116, 152, 536, 578
StyD4I CCNGG 1 cut(s) 198
StyI CCWWGG 1 cut(s) 60
TaaI ACNGT 5 cut(s) 794, 902, 1021, 1296, 1301
TaiI ACGT 2 cut(s) 59, 480
TaqI TCGA 5 cut(s) 279, 463, 828, 966, 1064
TaqII GACCGA 2 cut(s) 449, 1326
TasI AATT 2 cut(s) 861, 972
TatI WGTACW 3 cut(s) 240, 753, 1296
TauI GCSGC 5 cut(s) 98, 189, 258, 305, 492
TfiI GAWTC 3 cut(s) 934, 1069, 1099
Tru1I TTAA 5 cut(s) 516, 954, 1322, 1350, 1381
Tru9I TTAA 5 cut(s) 516, 954, 1322, 1350, 1381
TscAI CASTG 2 cut(s) 907, 912
TseFI GTSAC 2 cut(s) 430, 724
TseI GCWGC 4 cut(s) 92, 98, 183, 1366
Tsp45I GTSAC 2 cut(s) 430, 724
TspDTI ATGAA 2 cut(s) 456, 633
TspGWI ACGGA 2 cut(s) 931, 980
TspRI CASTG 2 cut(s) 907, 912
Tth111I GACNNNGTC 2 cut(s) 284, 792
VneI GTGCAC 1 cut(s) 903
VpaK11BI GGWCC 1 cut(s) 145
VspI ATTAAT 1 cut(s) 516
XagI CCTNNNNNAGG 1 cut(s) 1267
XbaI TCTAGA 1 cut(s) 151
XceI RCATGY 1 cut(s) 1109
XmiI GTMKAC 1 cut(s) 463
XspI CTAG 4 cut(s) 116, 152, 536, 578
ZraI GACGTC 1 cut(s) 57
ZrmI AGTACT 2 cut(s) 242, 1298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.