pycom420g00680

Ankyrin repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_420
OG0019748
Physical Location & Seq
Reverse (-)
811443 .. 811948
506 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom420g00680.2

Sequence Viewer

Length: 399 bp
ATGGCTGGAATCGTTGTGTCGAAGTATGATCATAGCCCTGTACACAAGGCCATAGTCACGAGGGATTATGCCGGTCTCAGGAGGATTCTAGCTGGCCTCCCGCGGCTTGGTGACCCTGCTGAGATTCGAACTGAATCAGCTTCATTAGCTGAGGAAGAGAAGGCTGATGCCATTGCTGCCGTCATTGACCGGCGAGATGTCCGAAACCGTGAAACCCCTCTTCACTTGGCTGTAAAACTTGGTGACCAGAAAAAGGAACATGGATGGAGTGCACTCCAGGAAGCAATTTGCAATAGAGAAGAAGCAATTTCCATGATCATAGTTAGGCCCTACCGGCCACTGGCTTGGGCAAAATGGTGCAGAAGGTTACCTCAGTTCTGTTATACCTTTCATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_5 GPCR_chapero_1

Protein Analysis

133

Amino Acids

15.05

Weight (kDa)

9.12

Isoelectric Point (pI)

52.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019748)

Species Orthologous Gene IDs
malus_domestica MD13G1284900.v1.1
prunus_persica Prupe.7G222900_v2.0.a1
pyrus_communis pycom420g00680
rosa_chinensis RchiOBHm_Chr2g0105151 RchiOBHm_Chr2g0107541 RchiOBHm_Chr3g0491521
rosa_samantha Rh2AG221900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 103
AciI CCGC 2 cut(s) 101, 103
AcoI YGGCCR 1 cut(s) 335
AfaI GTAC 1 cut(s) 42
AfiI CCNNNNNNNGG 4 cut(s) 78, 107, 253, 340
AjnI CCWGG 1 cut(s) 276
AluBI AGCT 3 cut(s) 92, 140, 149
AluI AGCT 3 cut(s) 92, 140, 149
Alw21I GWGCWC 1 cut(s) 274
Alw26I GTCTC 1 cut(s) 80
Alw44I GTGCAC 1 cut(s) 270
AoxI GGCC 4 cut(s) 48, 94, 326, 335
ApaLI GTGCAC 1 cut(s) 270
ApeKI GCWGC 1 cut(s) 176
AspS9I GGNCC 1 cut(s) 327
AsuHPI GGTGA 2 cut(s) 122, 254
AsuII TTCGAA 1 cut(s) 127
BaeGI GKGCMC 1 cut(s) 274
BauI CACGAG 1 cut(s) 58
Bbv12I GWGCWC 1 cut(s) 274
BbvCI CCTCAGC 1 cut(s) 150
BbvI GCAGC 1 cut(s) 163
BccI CCATC 1 cut(s) 258
BceAI ACGGC 1 cut(s) 164
BciT130I CCWGG 1 cut(s) 278
BclI TGATCA 2 cut(s) 28, 315
BcoDI GTCTC 1 cut(s) 80
BfaI CTAG 2 cut(s) 89, 397
BglI GCCNNNNNGGC 1 cut(s) 334
BisI GCNGC 2 cut(s) 104, 177
BlsI GCNGC 2 cut(s) 105, 178
Bme1390I CCNGG 1 cut(s) 278
BmgT120I GGNCC 1 cut(s) 327
BmrFI CCNGG 1 cut(s) 278
BmsI GCATC 1 cut(s) 157
BpmI CTGGAG 1 cut(s) 260
Bpu10I CCTNAGC 1 cut(s) 150
Bpu14I TTCGAA 1 cut(s) 127
BsaI GGTCTC 1 cut(s) 80
BsaJI CCNNGG 1 cut(s) 101
Bsc4I CCNNNNNNNGG 4 cut(s) 78, 107, 253, 340
Bse118I RCCGGY 3 cut(s) 71, 189, 333
Bse1I ACTGG 1 cut(s) 345
Bse3DI GCAATG 1 cut(s) 171
BseBI CCWGG 1 cut(s) 278
BseDI CCNNGG 1 cut(s) 101
BseGI GGATG 1 cut(s) 269
BseLI CCNNNNNNNGG 4 cut(s) 78, 107, 253, 340
BseMI GCAATG 1 cut(s) 171
BseMII CTCAG 4 cut(s) 91, 111, 141, 386
BseNI ACTGG 1 cut(s) 345
BseSI GKGCMC 1 cut(s) 274
BseXI GCAGC 1 cut(s) 163
BsgI GTGCAG 1 cut(s) 379
Bsh1236I CGCG 1 cut(s) 103
BshFI GGCC 4 cut(s) 50, 96, 328, 337
BsiHKAI GWGCWC 1 cut(s) 274
BsiSI CCGG 3 cut(s) 72, 190, 334
BslI CCNNNNNNNGG 4 cut(s) 78, 107, 253, 340
BsmAI GTCTC 1 cut(s) 80
BsnI GGCC 4 cut(s) 50, 96, 328, 337
Bso31I GGTCTC 1 cut(s) 80
Bsp119I TTCGAA 1 cut(s) 127
Bsp1286I GDGCHC 1 cut(s) 274
Bsp1407I TGTACA 1 cut(s) 40
Bsp143I GATC 2 cut(s) 28, 315
BspACI CCGC 2 cut(s) 101, 103
BspANI GGCC 4 cut(s) 50, 96, 328, 337
BspCNI CTCAG 4 cut(s) 90, 112, 142, 385
BspFNI CGCG 1 cut(s) 103
BspT104I TTCGAA 1 cut(s) 127
BspTNI GGTCTC 1 cut(s) 80
BsrDI GCAATG 1 cut(s) 171
BsrFI RCCGGY 3 cut(s) 71, 189, 333
BsrGI TGTACA 1 cut(s) 40
BsrI ACTGG 1 cut(s) 345
BssAI RCCGGY 3 cut(s) 71, 189, 333
BssECI CCNNGG 1 cut(s) 101
BssMI GATC 2 cut(s) 28, 315
BssSI CACGAG 1 cut(s) 58
Bst2BI CACGAG 1 cut(s) 58
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 1 cut(s) 209
Bst6I CTCTTC 2 cut(s) 150, 225
BstAUI TGTACA 1 cut(s) 40
BstBI TTCGAA 1 cut(s) 127
BstC8I GCNNGC 1 cut(s) 94
BstDEI CTNAG 4 cut(s) 77, 120, 150, 372
BstDSI CCRYGG 1 cut(s) 101
BstEII GGTNACC 3 cut(s) 110, 242, 366
BstF5I GGATG 1 cut(s) 269
BstFNI CGCG 1 cut(s) 103
BstKTI GATC 2 cut(s) 31, 318
BstMAI GTCTC 1 cut(s) 80
BstMBI GATC 2 cut(s) 28, 315
BstMWI GCNNNNNNNGC 3 cut(s) 146, 176, 334
BstNI CCWGG 1 cut(s) 278
BstPI GGTNACC 3 cut(s) 110, 242, 366
BstSCI CCNGG 1 cut(s) 276
BstSLI GKGCMC 1 cut(s) 274
BstUI CGCG 1 cut(s) 103
BstV1I GCAGC 1 cut(s) 163
BstXI CCANNNNNNTGG 1 cut(s) 345
BsuRI GGCC 4 cut(s) 50, 96, 328, 337
BtgI CCRYGG 1 cut(s) 101
BtsCI GGATG 1 cut(s) 269
BtsIMutI CAGTG 1 cut(s) 338
Cac8I GCNNGC 1 cut(s) 94
Cfr10I RCCGGY 3 cut(s) 71, 189, 333
Cfr13I GGNCC 1 cut(s) 327
Cfr42I CCGCGG 1 cut(s) 104
Csp6I GTAC 1 cut(s) 41
CviAII CATG 2 cut(s) 260, 313
CviQI GTAC 1 cut(s) 41
DdeI CTNAG 4 cut(s) 77, 120, 150, 372
DpnI GATC 2 cut(s) 30, 317
DpnII GATC 2 cut(s) 28, 315
EaeI YGGCCR 1 cut(s) 335
Eam1104I CTCTTC 2 cut(s) 150, 225
EarI CTCTTC 2 cut(s) 150, 225
Eco31I GGTCTC 1 cut(s) 80
Eco91I GGTNACC 3 cut(s) 110, 242, 366
EcoO109I RGGNCCY 1 cut(s) 327
EcoO65I GGTNACC 3 cut(s) 110, 242, 366
EcoRII CCWGG 1 cut(s) 276
FaeI CATG 2 cut(s) 263, 316
FaiI YATR 8 cut(s) 27, 33, 53, 69, 261, 314, 320, 384
FatI CATG 2 cut(s) 259, 312
FauI CCCGC 1 cut(s) 108
FbaI TGATCA 2 cut(s) 28, 315
Fnu4HI GCNGC 2 cut(s) 104, 177
FokI GGATG 1 cut(s) 276
Fsp4HI GCNGC 2 cut(s) 104, 177
FspBI CTAG 2 cut(s) 89, 397
GluI GCNGC 2 cut(s) 104, 177
GsuI CTGGAG 1 cut(s) 260
HaeIII GGCC 4 cut(s) 50, 96, 328, 337
HapII CCGG 3 cut(s) 72, 190, 334
Hin1II CATG 2 cut(s) 263, 316
HinfI GANTC 4 cut(s) 9, 85, 124, 134
HpaII CCGG 3 cut(s) 72, 190, 334
HphI GGTGA 2 cut(s) 122, 254
Hpy166II GTNNAC 2 cut(s) 43, 272
Hpy188I TCNGA 1 cut(s) 203
Hpy188III TCNNGA 2 cut(s) 58, 79
Hpy8I GTNNAC 2 cut(s) 43, 272
HpyAV CCTTC 2 cut(s) 154, 357
HpyCH4III ACNGT 1 cut(s) 209
HpyCH4V TGCA 3 cut(s) 272, 291, 360
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 176, 334
HpyF3I CTNAG 4 cut(s) 77, 120, 150, 372
Hsp92II CATG 2 cut(s) 263, 316
Ksp22I TGATCA 2 cut(s) 28, 315
KspI CCGCGG 1 cut(s) 104
Kzo9I GATC 2 cut(s) 28, 315
Lsp1109I GCAGC 1 cut(s) 163
LweI GCATC 1 cut(s) 157
MaeI CTAG 2 cut(s) 89, 397
MaeIII GTNAC 4 cut(s) 55, 110, 242, 366
MalI GATC 2 cut(s) 30, 317
MboI GATC 2 cut(s) 28, 315
MboII GAAGA 3 cut(s) 167, 212, 311
MhlI GDGCHC 1 cut(s) 274
MluCI AATT 2 cut(s) 285, 306
MnlI CCTC 6 cut(s) 54, 75, 107, 145, 228, 381
MspA1I CMGCKG 1 cut(s) 103
MspI CCGG 3 cut(s) 72, 190, 334
MspR9I CCNGG 1 cut(s) 278
MvaI CCWGG 1 cut(s) 278
MvnI CGCG 1 cut(s) 103
MwoI GCNNNNNNNGC 3 cut(s) 146, 176, 334
NdeII GATC 2 cut(s) 28, 315
NlaIII CATG 2 cut(s) 263, 316
NmuCI GTSAC 3 cut(s) 55, 110, 242
NspV TTCGAA 1 cut(s) 127
PfeI GAWTC 4 cut(s) 9, 85, 124, 134
PfoI TCCNGGA 1 cut(s) 276
PkrI GCNGC 2 cut(s) 105, 178
Psp6I CCWGG 1 cut(s) 276
PspEI GGTNACC 3 cut(s) 110, 242, 366
PspGI CCWGG 1 cut(s) 276
PspPI GGNCC 1 cut(s) 327
RsaI GTAC 1 cut(s) 42
RsaNI GTAC 1 cut(s) 41
SacII CCGCGG 1 cut(s) 104
SatI GCNGC 2 cut(s) 104, 177
Sau3AI GATC 2 cut(s) 28, 315
Sau96I GGNCC 1 cut(s) 327
ScrFI CCNGG 1 cut(s) 278
SduI GDGCHC 1 cut(s) 274
SetI ASST 6 cut(s) 94, 142, 151, 368, 373, 389
SfaNI GCATC 1 cut(s) 157
SfiI GGCCNNNNNGGCC 1 cut(s) 334
Sfr303I CCGCGG 1 cut(s) 104
SfuI TTCGAA 1 cut(s) 127
SgrBI CCGCGG 1 cut(s) 104
Sse9I AATT 2 cut(s) 285, 306
SsiI CCGC 2 cut(s) 101, 103
SspMI CTAG 2 cut(s) 89, 397
StyD4I CCNGG 1 cut(s) 276
TaaI ACNGT 1 cut(s) 209
TaqI TCGA 2 cut(s) 20, 127
TasI AATT 2 cut(s) 285, 306
TatI WGTACW 1 cut(s) 40
TauI GCSGC 1 cut(s) 106
TfiI GAWTC 4 cut(s) 9, 85, 124, 134
TscAI CASTG 1 cut(s) 345
TseFI GTSAC 3 cut(s) 55, 110, 242
TseI GCWGC 1 cut(s) 176
Tsp45I GTSAC 3 cut(s) 55, 110, 242
TspDTI ATGAA 2 cut(s) 132, 380
TspRI CASTG 1 cut(s) 345
VneI GTGCAC 1 cut(s) 270
XspI CTAG 2 cut(s) 89, 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.