pycom849g00010

3beta-hydroxysteroid-dehydrogenase decarboxylase isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000849
OG0018331
Physical Location & Seq
Reverse (-)
23785 .. 25545
1761 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 414 bp
ATGATAAAATACAATGATTCTATGTCAGCGCAATACATTCGATTATCTTCACGCACTAGAACGTTCGACTGCACTGCGGCTCAAAATTTGCAATGGCATGATGAGAAGAAATCTTTTACATATTTCCTTGCTTTGAGTATGTTGTATTACTGGTTTTTCCTTTCTGGAAGAACTTTTGCCTCATCTATGTCCAAGCTTCTGCTGTTTGTTTCATTTGTGAACAGGTTGGGTTTTACCCTTAAGACGCTATCTTTATCTTGTTTTGAGATTCCTGAAACGGTAGTGAAAGATAAGATAGCAAGCATAGCATATTTGTGGAACAAAGGGGTTTGTTATTTGAGATTACTGGCCCGAGGACATGGCTGGGATATTTTCTTCAAGGTATGGACCATAATAACTCATTCCCTTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01130 map00100 map01100 map01130
Pfam Domains
Protein Families
3Beta_HSD Reticulon

Protein Analysis

138

Amino Acids

16.11

Weight (kDa)

9.71

Isoelectric Point (pI)

35.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 30 - 131 1.5e-12 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 77
AclI AACGTT 1 cut(s) 62
AcsI RAATTY 1 cut(s) 85
AflII CTTAAG 1 cut(s) 239
AgsI TTSAA 1 cut(s) 379
AluBI AGCT 1 cut(s) 196
AluI AGCT 1 cut(s) 196
Ama87I CYCGRG 1 cut(s) 351
AoxI GGCC 1 cut(s) 348
ApoI RAATTY 1 cut(s) 85
AspLEI GCGC 1 cut(s) 31
AspS9I GGNCC 2 cut(s) 349, 387
AvaI CYCGRG 1 cut(s) 351
AvaII GGWCC 1 cut(s) 387
BcgI CGANNNNNNTGC 4 cut(s) 20, 54, 56, 90
BfaI CTAG 1 cut(s) 57
BfrI CTTAAG 1 cut(s) 239
BisI GCNGC 1 cut(s) 78
BlsI GCNGC 1 cut(s) 79
Bme18I GGWCC 1 cut(s) 387
BmeT110I CYCGRG 1 cut(s) 351
BmgT120I GGNCC 2 cut(s) 349, 387
BsaJI CCNNGG 1 cut(s) 352
Bse1I ACTGG 2 cut(s) 155, 351
Bse3DI GCAATG 1 cut(s) 98
BseDI CCNNGG 1 cut(s) 352
BseMI GCAATG 1 cut(s) 98
BseNI ACTGG 2 cut(s) 155, 351
BseYI CCCAGC 1 cut(s) 363
BsgI GTGCAG 1 cut(s) 55
BshFI GGCC 1 cut(s) 350
BsiHKCI CYCGRG 1 cut(s) 351
BsnI GGCC 1 cut(s) 350
BsoBI CYCGRG 1 cut(s) 351
BspACI CCGC 1 cut(s) 77
BspANI GGCC 1 cut(s) 350
BspTI CTTAAG 1 cut(s) 239
BsrDI GCAATG 1 cut(s) 98
BsrI ACTGG 2 cut(s) 155, 351
BssECI CCNNGG 1 cut(s) 352
Bst4CI ACNGT 1 cut(s) 280
BstAFI CTTAAG 1 cut(s) 239
BstC8I GCNNGC 1 cut(s) 301
BstHHI GCGC 1 cut(s) 31
BstMWI GCNNNNNNNGC 1 cut(s) 305
BsuRI GGCC 1 cut(s) 350
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 1 cut(s) 72
Cac8I GCNNGC 1 cut(s) 301
CfoI GCGC 1 cut(s) 31
Cfr13I GGNCC 2 cut(s) 349, 387
CseI GACGC 1 cut(s) 253
CviAII CATG 2 cut(s) 98, 359
CviJI RGCY 4 cut(s) 80, 196, 350, 363
CviKI_1 RGCY 4 cut(s) 80, 196, 350, 363
Eco47I GGWCC 1 cut(s) 387
Eco88I CYCGRG 1 cut(s) 351
FaeI CATG 2 cut(s) 101, 362
FatI CATG 2 cut(s) 97, 358
Fnu4HI GCNGC 1 cut(s) 78
Fsp4HI GCNGC 1 cut(s) 78
FspBI CTAG 1 cut(s) 57
GlaI GCGC 1 cut(s) 30
GluI GCNGC 1 cut(s) 78
GsaI CCCAGC 1 cut(s) 367
HaeIII GGCC 1 cut(s) 350
HgaI GACGC 1 cut(s) 253
HhaI GCGC 1 cut(s) 31
Hin1II CATG 2 cut(s) 101, 362
Hin6I GCGC 1 cut(s) 29
HinP1I GCGC 1 cut(s) 29
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 2 cut(s) 17, 268
Hpy166II GTNNAC 1 cut(s) 220
Hpy188III TCNNGA 2 cut(s) 165, 272
Hpy8I GTNNAC 1 cut(s) 220
HpyCH4III ACNGT 1 cut(s) 280
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 2 cut(s) 72, 91
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpySE526I ACGT 1 cut(s) 62
Hsp92II CATG 2 cut(s) 101, 362
HspAI GCGC 1 cut(s) 29
LpnPI CCDG 6 cut(s) 136, 150, 208, 285, 332, 349
MaeI CTAG 1 cut(s) 57
MaeII ACGT 1 cut(s) 62
MboII GAAGA 4 cut(s) 39, 118, 180, 367
MluCI AATT 1 cut(s) 85
MnlI CCTC 2 cut(s) 190, 347
MseI TTAA 1 cut(s) 240
MslI CAYNNNNRTG 1 cut(s) 313
MspCI CTTAAG 1 cut(s) 239
MwoI GCNNNNNNNGC 1 cut(s) 305
NlaIII CATG 2 cut(s) 101, 362
PfeI GAWTC 2 cut(s) 17, 268
PkrI GCNGC 1 cut(s) 79
Psp1406I AACGTT 1 cut(s) 62
PspFI CCCAGC 1 cut(s) 363
PspPI GGNCC 2 cut(s) 349, 387
RseI CAYNNNNRTG 1 cut(s) 313
SaqAI TTAA 1 cut(s) 240
SatI GCNGC 1 cut(s) 78
Sau96I GGNCC 2 cut(s) 349, 387
SetI ASST 4 cut(s) 65, 198, 227, 384
SinI GGWCC 1 cut(s) 387
SmiMI CAYNNNNRTG 1 cut(s) 313
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 1 cut(s) 85
SsiI CCGC 1 cut(s) 77
SspMI CTAG 1 cut(s) 57
TaaI ACNGT 1 cut(s) 280
TaiI ACGT 1 cut(s) 65
TaqI TCGA 2 cut(s) 40, 66
TasI AATT 1 cut(s) 85
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 2 cut(s) 17, 268
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TscAI CASTG 1 cut(s) 79
TspDTI ATGAA 1 cut(s) 201
TspRI CASTG 1 cut(s) 79
Vha464I CTTAAG 1 cut(s) 239
VpaK11BI GGWCC 1 cut(s) 387
XapI RAATTY 1 cut(s) 85
XspI CTAG 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.