RchiOBHm_Chr7g0179271

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0014744
Physical Location & Seq
Forward (+)
1602823 .. 1604961
2139 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 780 bp
ATGGTTAGCTTAGACTGTGTGCTAGTACTAGCGGGTCTGTTTGTGTTGGGAATATACCATGTTTGGCTATTCATCACAGTTCTGCACAATCCCATAAGAACTGTCATTGGCCTCAATGCTTACACTCGTCAACAATGGGTTTTCTCCCTGATGACTGACCCCTTGAAGAATGGTGTTTTGATTGTACAAACTTTACGCAACAACATAATGGCATCGACACTTTTGTCAACAGCAGCAATTACTCTTTGTTCTGTGATCAGTGTACTTGTGAGCAGCGAATCCAGCTCCCATAGCACTGTATCAGTACACCTAGTTTACTCCGATTACGCTCCCTCCTTACCTTCAATCAAGTACTTGTGCATTTTGCTTTGCTTTCTTGTTGCATTTCTCTGCAATGTGCAGTCTACTAGATATTATGCCCATGTCAGCTTCTTGGGTACCGTCCCAACATGGAAAGACAAGACGGGTTTCATCGAGTATGTTGCGGCAACTTTGAATAGAGGATATTACTTTTGGGCCCTTGGATTGCGAGCATTCTACCTCTCGATTCCTCTCTTTCTCTGGATCTTCGGGCCAATACCCATGTTTGTTAGTTGCTGTGTATTGTCAATTGTTCTCTATTTCTTGGACACTACCTCCAGCTTTGCACAACTTATTCATACCCAATTGTTCAAGGAGGAATCAAGAACCGCTGATGTGGAGTCCACCGCTCAATCATTGAACAATTCTGGTCGTGAGAACTCCCAACTTCGTGATCCTCTACTCACCGGGTCCGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

259

Amino Acids

28.85

Weight (kDa)

7.61

Isoelectric Point (pI)

32.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 7 - 214 1.8e-64 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 223
Acc65I GGTACC 1 cut(s) 437
AccB1I GGYRCC 1 cut(s) 437
AccBSI CCGCTC 1 cut(s) 710
AccI GTMKAC 1 cut(s) 404
AciI CCGC 4 cut(s) 32, 485, 690, 708
AclWI GGATC 2 cut(s) 572, 749
AfaI GTAC 6 cut(s) 27, 186, 264, 306, 353, 439
AgsI TTSAA 5 cut(s) 166, 345, 496, 673, 721
AluBI AGCT 4 cut(s) 9, 285, 429, 642
AluI AGCT 4 cut(s) 9, 285, 429, 642
AlwI GGATC 2 cut(s) 572, 749
AoxI GGCC 3 cut(s) 109, 516, 572
ApaI GGGCCC 1 cut(s) 520
ApeKI GCWGC 2 cut(s) 233, 273
Asp718I GGTACC 1 cut(s) 437
AspS9I GGNCC 4 cut(s) 516, 517, 572, 771
AsuC2I CCSGG 1 cut(s) 769
AsuHPI GGTGA 1 cut(s) 757
AvaII GGWCC 1 cut(s) 771
BaeGI GKGCMC 1 cut(s) 520
BanI GGYRCC 1 cut(s) 437
BanII GRGCYC 1 cut(s) 520
BbvI GCAGC 2 cut(s) 245, 285
BcgI CGANNNNNNTGC 2 cut(s) 464, 498
BclI TGATCA 1 cut(s) 255
BcnI CCSGG 1 cut(s) 769
BfaI CTAG 5 cut(s) 23, 29, 311, 408, 778
BisI GCNGC 3 cut(s) 234, 274, 486
BlsI GCNGC 3 cut(s) 235, 275, 487
BmcAI AGTACT 2 cut(s) 27, 353
Bme1390I CCNGG 1 cut(s) 769
Bme18I GGWCC 1 cut(s) 771
BmgT120I GGNCC 4 cut(s) 516, 517, 572, 771
BmiI GGNNCC 3 cut(s) 439, 518, 772
BmrFI CCNGG 1 cut(s) 769
BmsI GCATC 1 cut(s) 221
BpmI CTGGAG 1 cut(s) 622
BpuMI CCSGG 1 cut(s) 769
BsaJI CCNNGG 1 cut(s) 520
BsaXI ACNNNNNCTCC 4 cut(s) 317, 347, 620, 650
Bse3DI GCAATG 1 cut(s) 400
BseDI CCNNGG 1 cut(s) 520
BseMI GCAATG 1 cut(s) 400
BseSI GKGCMC 1 cut(s) 520
BseXI GCAGC 2 cut(s) 245, 285
BsgI GTGCAG 2 cut(s) 68, 419
BshFI GGCC 3 cut(s) 111, 518, 574
BshNI GGYRCC 1 cut(s) 437
BsiSI CCGG 2 cut(s) 768, 774
BslFI GGGAC 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 428
BsmI GAATGC 1 cut(s) 533
BsnI GGCC 3 cut(s) 111, 518, 574
Bsp120I GGGCCC 1 cut(s) 516
Bsp1286I GDGCHC 1 cut(s) 520
Bsp1407I TGTACA 1 cut(s) 184
Bsp143I GATC 3 cut(s) 255, 564, 754
BspACI CCGC 4 cut(s) 32, 485, 690, 708
BspANI GGCC 3 cut(s) 111, 518, 574
BspLI GGNNCC 3 cut(s) 439, 518, 772
BspPI GGATC 2 cut(s) 572, 749
BspT107I GGYRCC 1 cut(s) 437
BsrBI CCGCTC 1 cut(s) 710
BsrDI GCAATG 1 cut(s) 400
BsrGI TGTACA 1 cut(s) 184
BssECI CCNNGG 1 cut(s) 520
BssMI GATC 3 cut(s) 255, 564, 754
BssT1I CCWWGG 1 cut(s) 520
Bst4CI ACNGT 5 cut(s) 17, 79, 103, 298, 442
BstAUI TGTACA 1 cut(s) 184
BstC8I GCNNGC 1 cut(s) 531
BstDEI CTNAG 1 cut(s) 10
BstKTI GATC 3 cut(s) 258, 567, 757
BstMBI GATC 3 cut(s) 255, 564, 754
BstMWI GCNNNNNNNGC 2 cut(s) 282, 291
BstSCI CCNGG 1 cut(s) 767
BstSLI GKGCMC 1 cut(s) 520
BstV1I GCAGC 2 cut(s) 245, 285
BstX2I RGATCY 1 cut(s) 564
BstYI RGATCY 1 cut(s) 564
BsuRI GGCC 3 cut(s) 111, 518, 574
BtsIMutI CAGTG 2 cut(s) 265, 294
Cac8I GCNNGC 1 cut(s) 531
Cfr13I GGNCC 4 cut(s) 516, 517, 572, 771
Csp6I GTAC 6 cut(s) 26, 185, 263, 305, 352, 438
CviAII CATG 4 cut(s) 59, 422, 450, 583
CviJI RGCY 9 cut(s) 9, 67, 111, 285, 429, 518, 574, 642, 777
CviKI_1 RGCY 9 cut(s) 9, 67, 111, 285, 429, 518, 574, 642, 777
CviQI GTAC 6 cut(s) 26, 185, 263, 305, 352, 438
DdeI CTNAG 1 cut(s) 10
DpnI GATC 3 cut(s) 257, 566, 756
DpnII GATC 3 cut(s) 255, 564, 754
DrdI GACNNNNNNGTC 1 cut(s) 223
DseDI GACNNNNNNGTC 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 520
Eco24I GRGCYC 1 cut(s) 520
Eco47I GGWCC 1 cut(s) 771
EcoO109I RGGNCCY 1 cut(s) 517
EcoT14I CCWWGG 1 cut(s) 520
EcoT38I GRGCYC 1 cut(s) 520
ErhI CCWWGG 1 cut(s) 520
FaeI CATG 4 cut(s) 62, 425, 453, 586
FaqI GGGAC 1 cut(s) 428
FatI CATG 4 cut(s) 58, 421, 449, 582
FauI CCCGC 1 cut(s) 25
FbaI TGATCA 1 cut(s) 255
FblI GTMKAC 1 cut(s) 404
Fnu4HI GCNGC 3 cut(s) 234, 274, 486
FriOI GRGCYC 1 cut(s) 520
Fsp4HI GCNGC 3 cut(s) 234, 274, 486
FspBI CTAG 5 cut(s) 23, 29, 311, 408, 778
GluI GCNGC 3 cut(s) 234, 274, 486
GsuI CTGGAG 1 cut(s) 622
HaeIII GGCC 3 cut(s) 111, 518, 574
HapII CCGG 2 cut(s) 768, 774
Hin1II CATG 4 cut(s) 62, 425, 453, 586
HincII GTYRAC 2 cut(s) 131, 228
HindII GTYRAC 2 cut(s) 131, 228
HinfI GANTC 4 cut(s) 278, 547, 680, 701
HpaII CCGG 2 cut(s) 768, 774
HphI GGTGA 1 cut(s) 757
Hpy166II GTNNAC 7 cut(s) 131, 228, 263, 307, 316, 405, 705
Hpy188I TCNGA 1 cut(s) 322
Hpy188III TCNNGA 5 cut(s) 544, 562, 684, 734, 752
Hpy8I GTNNAC 7 cut(s) 131, 228, 263, 307, 316, 405, 705
HpyAV CCTTC 1 cut(s) 351
HpyCH4III ACNGT 5 cut(s) 17, 79, 103, 298, 442
HpyCH4V TGCA 6 cut(s) 85, 360, 383, 393, 400, 647
HpyF10VI GCNNNNNNNGC 2 cut(s) 282, 291
HpyF3I CTNAG 1 cut(s) 10
Hsp92II CATG 4 cut(s) 62, 425, 453, 586
KpnI GGTACC 1 cut(s) 441
Ksp22I TGATCA 1 cut(s) 255
Kzo9I GATC 3 cut(s) 255, 564, 754
LmnI GCTCC 2 cut(s) 290, 334
LpnPI CCDG 5 cut(s) 161, 295, 547, 652, 714
Lsp1109I GCAGC 2 cut(s) 245, 285
LweI GCATC 1 cut(s) 221
MaeI CTAG 5 cut(s) 23, 29, 311, 408, 778
MalI GATC 3 cut(s) 257, 566, 756
MbiI CCGCTC 1 cut(s) 710
MboI GATC 3 cut(s) 255, 564, 754
MboII GAAGA 2 cut(s) 178, 559
MfeI CAATTG 2 cut(s) 609, 665
MflI RGATCY 1 cut(s) 564
MhlI GDGCHC 1 cut(s) 520
MluCI AATT 4 cut(s) 237, 609, 665, 724
MlyI GAGTC 1 cut(s) 710
MnlI CCTC 8 cut(s) 122, 343, 494, 551, 561, 646, 670, 768
MspA1I CMGCKG 1 cut(s) 692
MspI CCGG 2 cut(s) 768, 774
MspR9I CCNGG 1 cut(s) 769
MunI CAATTG 2 cut(s) 609, 665
Mva1269I GAATGC 1 cut(s) 533
MwoI GCNNNNNNNGC 2 cut(s) 282, 291
NciI CCSGG 1 cut(s) 769
NdeII GATC 3 cut(s) 255, 564, 754
NlaIII CATG 4 cut(s) 62, 425, 453, 586
NlaIV GGNNCC 3 cut(s) 439, 518, 772
PctI GAATGC 1 cut(s) 533
PfeI GAWTC 3 cut(s) 278, 547, 680
PkrI GCNGC 3 cut(s) 235, 275, 487
PleI GAGTC 1 cut(s) 709
PpsI GAGTC 1 cut(s) 709
PspN4I GGNNCC 3 cut(s) 439, 518, 772
PspOMI GGGCCC 1 cut(s) 516
PspPI GGNCC 4 cut(s) 516, 517, 572, 771
PsuI RGATCY 1 cut(s) 564
RsaI GTAC 6 cut(s) 27, 186, 264, 306, 353, 439
RsaNI GTAC 6 cut(s) 26, 185, 263, 305, 352, 438
SatI GCNGC 3 cut(s) 234, 274, 486
Sau3AI GATC 3 cut(s) 255, 564, 754
Sau96I GGNCC 4 cut(s) 516, 517, 572, 771
ScaI AGTACT 2 cut(s) 27, 353
SchI GAGTC 1 cut(s) 710
ScrFI CCNGG 1 cut(s) 769
SduI GDGCHC 1 cut(s) 520
SetI ASST 8 cut(s) 11, 287, 312, 343, 431, 543, 638, 644
SfaNI GCATC 1 cut(s) 221
SinI GGWCC 1 cut(s) 771
Sse9I AATT 4 cut(s) 237, 609, 665, 724
SsiI CCGC 4 cut(s) 32, 485, 690, 708
SspMI CTAG 5 cut(s) 23, 29, 311, 408, 778
StyD4I CCNGG 1 cut(s) 767
StyI CCWWGG 1 cut(s) 520
TaaI ACNGT 5 cut(s) 17, 79, 103, 298, 442
TaqI TCGA 3 cut(s) 215, 474, 545
TasI AATT 4 cut(s) 237, 609, 665, 724
TatI WGTACW 5 cut(s) 25, 184, 262, 304, 351
TauI GCSGC 1 cut(s) 488
TfiI GAWTC 3 cut(s) 278, 547, 680
TscAI CASTG 2 cut(s) 265, 301
TseI GCWGC 2 cut(s) 233, 273
TspDTI ATGAA 3 cut(s) 61, 460, 647
TspRI CASTG 2 cut(s) 265, 301
VpaK11BI GGWCC 1 cut(s) 771
XmiI GTMKAC 1 cut(s) 404
XspI CTAG 5 cut(s) 23, 29, 311, 408, 778
ZrmI AGTACT 2 cut(s) 27, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.