RchiOBHm_Chr7g0199591

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0021483
Physical Location & Seq
Forward (+)
17679117 .. 17679658
542 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 495 bp
ATGCATGGTGGAGCACAGGAGTATTTTGGCATTACTCCTGATCTAACAACACTCGGCAAGATCATTGGTGGCGGTCTGCCAATTGGTGCATATGGCGGAAGGAGGGAGATAATGGAAATGGTAGCACCAGCAGGTCCAGTGTACCAAGCCGGGACCTTCTGCGGCAACCCATTGTCAATGGCTGCAGGGATTCAGACTCTTAAGAGGTTAAAGGAGCCAGGAAGTTGCGAACACCTGAATAGGATTACAAGTGAACTAATTCAAGGTATCATAGATGCCGGGAAGAGGGCTGGGCATGAAATCTGCGGTGGATGCATAAGTGGGATCTTGGGGAGTGACAGTGCAAAGTACGGGAGGTTTCATACGGGAATGCTGGAGGAAGGTGTATACTTTGCTCCTTCACAGTTTGAGGCCGGGCTTACAAGCATGGTACATACTCCCGAAGACATCCGAATCACAATAGCTGCTGCAGAGAAAGTTTTTAGGCAGACTTAG

Protein Analysis

164

Amino Acids

17.31

Weight (kDa)

6.19

Isoelectric Point (pI)

37.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_3 PF00202 5 - 133 4.8e-19 Aminotransferase class-III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0021483)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr7g0199591
rosa_multiflora Rmu_sc0009191.1_g000013
rosa_samantha Rh7AG180200 Rh7CG189800 Rh7DG183100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 122
AccI GTMKAC 1 cut(s) 387
AciI CCGC 4 cut(s) 72, 96, 162, 306
AclWI GGATC 1 cut(s) 332
AfaI GTAC 3 cut(s) 143, 350, 432
AfiI CCNNNNNNNGG 1 cut(s) 285
AflII CTTAAG 1 cut(s) 200
AgsI TTSAA 1 cut(s) 263
AjnI CCWGG 1 cut(s) 217
AluBI AGCT 1 cut(s) 464
AluI AGCT 1 cut(s) 464
Alw21I GWGCWC 1 cut(s) 16
AlwI GGATC 1 cut(s) 332
AoxI GGCC 1 cut(s) 411
ApeKI GCWGC 3 cut(s) 182, 464, 467
Asp700I GAANNNNTTC 1 cut(s) 258
AspS9I GGNCC 2 cut(s) 134, 153
AsuC2I CCSGG 3 cut(s) 151, 280, 415
AvaII GGWCC 2 cut(s) 134, 153
BbsI GAAGAC 1 cut(s) 450
Bbv12I GWGCWC 1 cut(s) 16
BbvI GCAGC 3 cut(s) 169, 451, 454
BciT130I CCWGG 1 cut(s) 219
BcnI CCSGG 3 cut(s) 151, 280, 415
BfmI CTRYAG 2 cut(s) 183, 468
BfrI CTTAAG 1 cut(s) 200
BfuAI ACCTGC 1 cut(s) 122
BisI GCNGC 4 cut(s) 163, 183, 465, 468
BlsI GCNGC 4 cut(s) 164, 184, 466, 469
Bme1390I CCNGG 4 cut(s) 151, 219, 280, 415
Bme18I GGWCC 2 cut(s) 134, 153
BmgT120I GGNCC 2 cut(s) 134, 153
BmiI GGNNCC 2 cut(s) 154, 216
BmrFI CCNGG 4 cut(s) 151, 219, 280, 415
BmsI GCATC 2 cut(s) 265, 302
BpiI GAAGAC 1 cut(s) 450
BpmI CTGGAG 1 cut(s) 395
BpuMI CCSGG 3 cut(s) 151, 280, 415
BsaXI ACNNNNNCTCC 2 cut(s) 325, 355
Bsc4I CCNNNNNNNGG 1 cut(s) 285
Bse1I ACTGG 1 cut(s) 137
BseBI CCWGG 1 cut(s) 219
BseGI GGATG 2 cut(s) 317, 447
BseLI CCNNNNNNNGG 1 cut(s) 285
BseNI ACTGG 1 cut(s) 137
BseXI GCAGC 3 cut(s) 169, 451, 454
BseYI CCCAGC 1 cut(s) 290
BshFI GGCC 1 cut(s) 413
BsiHKAI GWGCWC 1 cut(s) 16
BsiSI CCGG 3 cut(s) 150, 279, 414
BslFI GGGAC 1 cut(s) 166
BslI CCNNNNNNNGG 1 cut(s) 285
BsmFI GGGAC 1 cut(s) 166
BsmI GAATGC 1 cut(s) 375
BsnI GGCC 1 cut(s) 413
Bsp1286I GDGCHC 1 cut(s) 16
Bsp143I GATC 3 cut(s) 40, 60, 324
BspACI CCGC 4 cut(s) 72, 96, 162, 306
BspANI GGCC 1 cut(s) 413
BspLI GGNNCC 2 cut(s) 154, 216
BspMAI CTGCAG 2 cut(s) 187, 472
BspMI ACCTGC 1 cut(s) 122
BspPI GGATC 1 cut(s) 332
BspTI CTTAAG 1 cut(s) 200
BsrI ACTGG 1 cut(s) 137
BssMI GATC 3 cut(s) 40, 60, 324
BssNAI GTATAC 1 cut(s) 388
Bst1107I GTATAC 1 cut(s) 388
Bst2UI CCWGG 1 cut(s) 219
Bst4CI ACNGT 2 cut(s) 341, 405
Bst6I CTCTTC 1 cut(s) 278
BstAFI CTTAAG 1 cut(s) 200
BstDEI CTNAG 1 cut(s) 492
BstF5I GGATG 2 cut(s) 317, 447
BstKTI GATC 3 cut(s) 43, 63, 327
BstMBI GATC 3 cut(s) 40, 60, 324
BstMWI GCNNNNNNNGC 1 cut(s) 312
BstNI CCWGG 1 cut(s) 219
BstSCI CCNGG 4 cut(s) 149, 217, 278, 413
BstSFI CTRYAG 2 cut(s) 183, 468
BstV1I GCAGC 3 cut(s) 169, 451, 454
BstV2I GAAGAC 1 cut(s) 450
BstX2I RGATCY 1 cut(s) 324
BstYI RGATCY 1 cut(s) 324
BstZ17I GTATAC 1 cut(s) 388
BsuRI GGCC 1 cut(s) 413
BtsCI GGATG 2 cut(s) 317, 447
BtsIMutI CAGTG 2 cut(s) 144, 346
BveI ACCTGC 1 cut(s) 122
Cfr13I GGNCC 2 cut(s) 134, 153
Csp6I GTAC 3 cut(s) 142, 349, 431
CviAII CATG 3 cut(s) 5, 296, 427
CviJI RGCY 7 cut(s) 149, 182, 217, 290, 413, 418, 464
CviKI_1 RGCY 7 cut(s) 149, 182, 217, 290, 413, 418, 464
CviQI GTAC 3 cut(s) 142, 349, 431
DdeI CTNAG 1 cut(s) 492
DpnI GATC 3 cut(s) 42, 62, 326
DpnII GATC 3 cut(s) 40, 60, 324
Eam1104I CTCTTC 1 cut(s) 278
EarI CTCTTC 1 cut(s) 278
EciI GGCGGA 1 cut(s) 111
Eco47I GGWCC 2 cut(s) 134, 153
EcoO109I RGGNCCY 1 cut(s) 153
EcoRII CCWGG 1 cut(s) 217
EcoT22I ATGCAT 2 cut(s) 6, 317
FaeI CATG 3 cut(s) 8, 299, 430
FaqI GGGAC 1 cut(s) 166
FatI CATG 3 cut(s) 4, 295, 426
FauNDI CATATG 1 cut(s) 91
FblI GTMKAC 1 cut(s) 387
Fnu4HI GCNGC 4 cut(s) 163, 183, 465, 468
FokI GGATG 2 cut(s) 324, 434
Fsp4HI GCNGC 4 cut(s) 163, 183, 465, 468
GluI GCNGC 4 cut(s) 163, 183, 465, 468
GsaI CCCAGC 1 cut(s) 294
GsuI CTGGAG 1 cut(s) 395
HaeIII GGCC 1 cut(s) 413
HapII CCGG 3 cut(s) 150, 279, 414
Hin1II CATG 3 cut(s) 8, 299, 430
HinfI GANTC 3 cut(s) 190, 196, 453
HpaII CCGG 3 cut(s) 150, 279, 414
Hpy166II GTNNAC 3 cut(s) 142, 254, 388
Hpy188I TCNGA 2 cut(s) 195, 452
Hpy188III TCNNGA 2 cut(s) 38, 440
Hpy8I GTNNAC 3 cut(s) 142, 254, 388
HpyAV CCTTC 4 cut(s) 93, 166, 374, 408
HpyCH4III ACNGT 2 cut(s) 341, 405
HpyCH4V TGCA 6 cut(s) 4, 89, 185, 315, 344, 470
HpyF10VI GCNNNNNNNGC 1 cut(s) 312
HpyF3I CTNAG 1 cut(s) 492
Hsp92II CATG 3 cut(s) 8, 299, 430
Kzo9I GATC 3 cut(s) 40, 60, 324
LmnI GCTCC 3 cut(s) 11, 214, 400
Lsp1109I GCAGC 3 cut(s) 169, 451, 454
LweI GCATC 2 cut(s) 265, 302
MaeIII GTNAC 1 cut(s) 335
MalI GATC 3 cut(s) 42, 62, 326
MboI GATC 3 cut(s) 40, 60, 324
MboII GAAGA 2 cut(s) 295, 455
MfeI CAATTG 1 cut(s) 81
MflI RGATCY 1 cut(s) 324
MhlI GDGCHC 1 cut(s) 16
MluCI AATT 2 cut(s) 81, 258
MlyI GAGTC 1 cut(s) 190
MnlI CCTC 6 cut(s) 96, 198, 279, 348, 370, 403
Mph1103I ATGCAT 2 cut(s) 6, 317
MroXI GAANNNNTTC 1 cut(s) 258
MseI TTAA 2 cut(s) 201, 209
MspCI CTTAAG 1 cut(s) 200
MspI CCGG 3 cut(s) 150, 279, 414
MspR9I CCNGG 4 cut(s) 151, 219, 280, 415
MunI CAATTG 1 cut(s) 81
Mva1269I GAATGC 1 cut(s) 375
MvaI CCWGG 1 cut(s) 219
MwoI GCNNNNNNNGC 1 cut(s) 312
NciI CCSGG 3 cut(s) 151, 280, 415
NdeI CATATG 1 cut(s) 91
NdeII GATC 3 cut(s) 40, 60, 324
NlaIII CATG 3 cut(s) 8, 299, 430
NlaIV GGNNCC 2 cut(s) 154, 216
NmeAIII GCCGAG 1 cut(s) 33
NmuCI GTSAC 1 cut(s) 335
NsiI ATGCAT 2 cut(s) 6, 317
PctI GAATGC 1 cut(s) 375
PdmI GAANNNNTTC 1 cut(s) 258
PfeI GAWTC 2 cut(s) 190, 453
PkrI GCNGC 4 cut(s) 164, 184, 466, 469
PleI GAGTC 1 cut(s) 190
PpsI GAGTC 1 cut(s) 190
PpuMI RGGWCCY 1 cut(s) 153
Psp5II RGGWCCY 1 cut(s) 153
Psp6I CCWGG 1 cut(s) 217
PspFI CCCAGC 1 cut(s) 290
PspGI CCWGG 1 cut(s) 217
PspN4I GGNNCC 2 cut(s) 154, 216
PspPI GGNCC 2 cut(s) 134, 153
PspPPI RGGWCCY 1 cut(s) 153
PstI CTGCAG 2 cut(s) 187, 472
PsuI RGATCY 1 cut(s) 324
RsaI GTAC 3 cut(s) 143, 350, 432
RsaNI GTAC 3 cut(s) 142, 349, 431
SaqAI TTAA 2 cut(s) 201, 209
SatI GCNGC 4 cut(s) 163, 183, 465, 468
Sau3AI GATC 3 cut(s) 40, 60, 324
Sau96I GGNCC 2 cut(s) 134, 153
SchI GAGTC 1 cut(s) 190
ScrFI CCNGG 4 cut(s) 151, 219, 280, 415
SduI GDGCHC 1 cut(s) 16
SetI ASST 8 cut(s) 136, 158, 209, 237, 268, 359, 385, 466
SfaNI GCATC 2 cut(s) 265, 302
SfcI CTRYAG 2 cut(s) 183, 468
SinI GGWCC 2 cut(s) 134, 153
SmlI CTYRAG 1 cut(s) 200
SmoI CTYRAG 1 cut(s) 200
Sse9I AATT 2 cut(s) 81, 258
SsiI CCGC 4 cut(s) 72, 96, 162, 306
StyD4I CCNGG 4 cut(s) 149, 217, 278, 413
TaaI ACNGT 2 cut(s) 341, 405
TasI AATT 2 cut(s) 81, 258
TauI GCSGC 1 cut(s) 165
TfiI GAWTC 2 cut(s) 190, 453
Tru1I TTAA 2 cut(s) 201, 209
Tru9I TTAA 2 cut(s) 201, 209
TscAI CASTG 2 cut(s) 144, 346
TseFI GTSAC 1 cut(s) 335
TseI GCWGC 3 cut(s) 182, 464, 467
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 2 cut(s) 312, 350
TspRI CASTG 2 cut(s) 144, 346
Vha464I CTTAAG 1 cut(s) 200
VpaK11BI GGWCC 2 cut(s) 134, 153
XmiI GTMKAC 1 cut(s) 387
XmnI GAANNNNTTC 1 cut(s) 258
Zsp2I ATGCAT 2 cut(s) 6, 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.