RchiOBHm_Chr7g0242231

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
Physical Location & Seq
Forward (+)
68395289 .. 68396646
1358 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 261 bp
ATGTTATTGCCGCCGCTCTCTTTTGACCGCTTCAACTTTCACTCAACTTTCCCGATGGCGCTTCGTTTCGAGGTTTTGGGGAGGTTCAATCGCGCTCGTGCAGCTCAACTGACACTCCCTCACTTTGTGTGTCAGACTCCTCTTTTCATGCCTGCTGGAGCACAAGGAACTATAAAAAGGTTGGCAACTGACCAGCTTGAGGAGATTGGTTGCCAAATAATTCTTGGGAATACATATCATTTGGCACTTCGACCTTATTGA

Protein Analysis

86

Amino Acids

9.79

Weight (kDa)

9.38

Isoelectric Point (pI)

39.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 31 - 85 2.8e-17 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Orthologous Genes (Group: OG0021090)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g36560
rosa_chinensis RchiOBHm_Chr7g0242231
rosa_multiflora Rmu_sc0000429.1_g000027
rosa_samantha Rh1DG069500
rosa_wichuraiana Rw0G009470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 16
AccII CGCG 1 cut(s) 93
AciI CCGC 3 cut(s) 11, 14, 28
AdeI CACNNNGTG 1 cut(s) 127
AfiI CCNNNNNNNGG 1 cut(s) 199
AgsI TTSAA 2 cut(s) 34, 88
AluBI AGCT 2 cut(s) 104, 196
AluI AGCT 2 cut(s) 104, 196
Alw21I GWGCWC 1 cut(s) 163
ApeKI GCWGC 1 cut(s) 101
AspLEI GCGC 2 cut(s) 61, 95
BauI CACGAG 1 cut(s) 96
Bbv12I GWGCWC 1 cut(s) 163
BbvI GCAGC 1 cut(s) 113
BccI CCATC 1 cut(s) 49
BfoI RGCGCY 1 cut(s) 62
BisI GCNGC 3 cut(s) 11, 14, 102
BlsI GCNGC 3 cut(s) 12, 15, 103
BpmI CTGGAG 1 cut(s) 177
BpuEI CTTGAG 1 cut(s) 218
BsaXI ACNNNNNCTCC 2 cut(s) 99, 129
Bsc4I CCNNNNNNNGG 1 cut(s) 199
BseLI CCNNNNNNNGG 1 cut(s) 199
BseRI GAGGAG 2 cut(s) 129, 215
BseXI GCAGC 1 cut(s) 113
BsgI GTGCAG 1 cut(s) 120
Bsh1236I CGCG 1 cut(s) 93
BsiHKAI GWGCWC 1 cut(s) 163
BslI CCNNNNNNNGG 1 cut(s) 199
Bsp1286I GDGCHC 1 cut(s) 163
BspACI CCGC 3 cut(s) 11, 14, 28
BspFNI CGCG 1 cut(s) 93
BsrBI CCGCTC 1 cut(s) 16
BssSI CACGAG 1 cut(s) 96
Bst2BI CACGAG 1 cut(s) 96
BstC8I GCNNGC 1 cut(s) 153
BstFNI CGCG 1 cut(s) 93
BstH2I RGCGCY 1 cut(s) 62
BstHHI GCGC 2 cut(s) 61, 95
BstMWI GCNNNNNNNGC 1 cut(s) 101
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 1 cut(s) 113
Cac8I GCNNGC 1 cut(s) 153
CfoI GCGC 2 cut(s) 61, 95
CviAII CATG 1 cut(s) 148
CviJI RGCY 2 cut(s) 104, 196
CviKI_1 RGCY 2 cut(s) 104, 196
DraIII CACNNNGTG 1 cut(s) 127
FaeI CATG 1 cut(s) 151
FaiI YATR 3 cut(s) 149, 173, 235
FatI CATG 1 cut(s) 147
Fnu4HI GCNGC 3 cut(s) 11, 14, 102
Fsp4HI GCNGC 3 cut(s) 11, 14, 102
GlaI GCGC 2 cut(s) 60, 94
GluI GCNGC 3 cut(s) 11, 14, 102
GsuI CTGGAG 1 cut(s) 177
HaeII RGCGCY 1 cut(s) 62
HhaI GCGC 2 cut(s) 61, 95
Hin1II CATG 1 cut(s) 151
Hin6I GCGC 2 cut(s) 59, 93
HinP1I GCGC 2 cut(s) 59, 93
HinfI GANTC 1 cut(s) 136
Hpy188I TCNGA 1 cut(s) 135
Hpy188III TCNNGA 1 cut(s) 52
HpyCH4V TGCA 1 cut(s) 101
HpyF10VI GCNNNNNNNGC 1 cut(s) 101
Hsp92II CATG 1 cut(s) 151
HspAI GCGC 2 cut(s) 59, 93
LmnI GCTCC 1 cut(s) 158
LpnPI CCDG 3 cut(s) 141, 165, 206
Lsp1109I GCAGC 1 cut(s) 113
MbiI CCGCTC 1 cut(s) 16
MhlI GDGCHC 1 cut(s) 163
MluCI AATT 1 cut(s) 219
MlyI GAGTC 1 cut(s) 130
MnlI CCTC 5 cut(s) 64, 75, 129, 150, 193
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 1 cut(s) 101
NlaIII CATG 1 cut(s) 151
PkrI GCNGC 3 cut(s) 12, 15, 103
PleI GAGTC 1 cut(s) 130
PpsI GAGTC 1 cut(s) 130
SatI GCNGC 3 cut(s) 11, 14, 102
SchI GAGTC 1 cut(s) 130
SduI GDGCHC 1 cut(s) 163
SetI ASST 6 cut(s) 75, 86, 106, 182, 198, 256
SmlI CTYRAG 1 cut(s) 197
SmoI CTYRAG 1 cut(s) 197
Sse9I AATT 1 cut(s) 219
SsiI CCGC 3 cut(s) 11, 14, 28
TaqI TCGA 2 cut(s) 69, 250
TasI AATT 1 cut(s) 219
TauI GCSGC 2 cut(s) 13, 16
TseI GCWGC 1 cut(s) 101
TspDTI ATGAA 1 cut(s) 136
XcmI CCANNNNNNNNNTGG 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.