Rw0G009470

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00371
OG0021090
Physical Location & Seq
Forward (+)
84563 .. 90628
6066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G009470.1

Sequence Viewer

Length: 513 bp
ATGGCGCTTCGTTTCGAGGTTTTAGGGAGGTTCAATCGCGCTCGTGCAGCTCAACTGACACTCCCTCACTTTGTGTGTCAGACTCCTCTTTTCATGCCTGTTGGAGCACAAGTTAGAACTATAAAAGGGTTGGCAACTGACCAGCTTGAGGAGATTGGTTGCCAAATAATTCTTGGGAATACATATCATTTGATGGGAGGTCTCCACAAATTTATGAACTGGCCAAGAGCGCAGCTTACTGATTCTGGTGGCTTTCAGATGGTATCCTTATTGCATTTAGCTGACATCACTGAGAAGGGTGTAACATTTCAGTCACCAGTCGATGGAAAGCCAATGCTCTTGACACCTGAGGAATTAATTCAAATCCAAAATAGAATTGGAGCAGATATTATAATGGCCCTTGATGATGTAGTGAAAACCACCATCACTGGTCCAAGAATTGAGGAGGCCATGTATCGTACTCTACGATGGATTGACAAATGTATAGCAGGCGGAACTTTCCCCAAAGTATGA

Protein Analysis

170

Amino Acids

18.94

Weight (kDa)

7.76

Isoelectric Point (pI)

35.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 13 - 163 1.4e-55 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0021090)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g36560
rosa_chinensis RchiOBHm_Chr7g0242231
rosa_multiflora Rmu_sc0000429.1_g000027
rosa_samantha Rh1DG069500
rosa_wichuraiana Rw0G009470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 392
AccB7I CCANNNNNTGG 1 cut(s) 323
AccII CGCG 1 cut(s) 39
AciI CCGC 1 cut(s) 492
AcoI YGGCCR 1 cut(s) 221
AcsI RAATTY 1 cut(s) 209
AdeI CACNNNGTG 1 cut(s) 73
AfaI GTAC 1 cut(s) 460
AfiI CCNNNNNNNGG 2 cut(s) 148, 323
AgsI TTSAA 2 cut(s) 34, 362
AluBI AGCT 4 cut(s) 50, 145, 235, 281
AluI AGCT 4 cut(s) 50, 145, 235, 281
Alw21I GWGCWC 1 cut(s) 109
Alw26I GTCTC 1 cut(s) 206
AoxI GGCC 3 cut(s) 221, 396, 447
ApeKI GCWGC 2 cut(s) 47, 232
ApoI RAATTY 1 cut(s) 209
AseI ATTAAT 1 cut(s) 356
Asp700I GAANNNNTTC 1 cut(s) 357
AspLEI GCGC 3 cut(s) 7, 41, 232
AspS9I GGNCC 2 cut(s) 397, 431
AsuHPI GGTGA 1 cut(s) 306
AvaII GGWCC 1 cut(s) 431
AxyI CCTNAGG 1 cut(s) 348
BalI TGGCCA 1 cut(s) 223
BauI CACGAG 1 cut(s) 42
Bbv12I GWGCWC 1 cut(s) 109
BbvI GCAGC 2 cut(s) 59, 244
BccI CCATC 5 cut(s) 187, 253, 317, 431, 462
BciVI GTATCC 1 cut(s) 274
BcoDI GTCTC 1 cut(s) 206
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 1 cut(s) 274
BisI GCNGC 2 cut(s) 48, 233
BlsI GCNGC 2 cut(s) 49, 234
Bme18I GGWCC 1 cut(s) 431
BmgT120I GGNCC 2 cut(s) 397, 431
BpuEI CTTGAG 1 cut(s) 167
BsaI GGTCTC 1 cut(s) 206
BsaXI ACNNNNNCTCC 6 cut(s) 45, 75, 96, 126, 437, 467
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 323
Bse1I ACTGG 3 cut(s) 224, 317, 433
Bse21I CCTNAGG 1 cut(s) 348
BseLI CCNNNNNNNGG 2 cut(s) 148, 323
BseMII CTCAG 2 cut(s) 282, 339
BseNI ACTGG 3 cut(s) 224, 317, 433
BseRI GAGGAG 3 cut(s) 75, 164, 458
BseXI GCAGC 2 cut(s) 59, 244
BsgI GTGCAG 1 cut(s) 66
Bsh1236I CGCG 1 cut(s) 39
BshFI GGCC 3 cut(s) 223, 398, 449
BsiHKAI GWGCWC 1 cut(s) 109
BslI CCNNNNNNNGG 2 cut(s) 148, 323
BsmAI GTCTC 1 cut(s) 206
BsnI GGCC 3 cut(s) 223, 398, 449
Bso31I GGTCTC 1 cut(s) 206
Bsp1286I GDGCHC 1 cut(s) 109
BspACI CCGC 1 cut(s) 492
BspANI GGCC 3 cut(s) 223, 398, 449
BspCNI CTCAG 2 cut(s) 283, 340
BspFNI CGCG 1 cut(s) 39
BspTNI GGTCTC 1 cut(s) 206
BsrI ACTGG 3 cut(s) 224, 317, 433
BssSI CACGAG 1 cut(s) 42
Bst2BI CACGAG 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 490
BstDEI CTNAG 2 cut(s) 291, 348
BstFNI CGCG 1 cut(s) 39
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 41, 232
BstMAI GTCTC 1 cut(s) 206
BstMWI GCNNNNNNNGC 2 cut(s) 47, 229
BstUI CGCG 1 cut(s) 39
BstV1I GCAGC 2 cut(s) 59, 244
Bsu36I CCTNAGG 1 cut(s) 348
BsuI GTATCC 1 cut(s) 274
BsuRI GGCC 3 cut(s) 223, 398, 449
BtsIMutI CAGTG 2 cut(s) 288, 426
Cac8I GCNNGC 1 cut(s) 490
CfoI GCGC 3 cut(s) 7, 41, 232
Cfr13I GGNCC 2 cut(s) 397, 431
Csp6I GTAC 1 cut(s) 459
CviAII CATG 2 cut(s) 94, 451
CviJI RGCY 9 cut(s) 50, 145, 223, 235, 252, 281, 331, 398, 449
CviKI_1 RGCY 9 cut(s) 50, 145, 223, 235, 252, 281, 331, 398, 449
CviQI GTAC 1 cut(s) 459
DdeI CTNAG 2 cut(s) 291, 348
DraIII CACNNNGTG 1 cut(s) 73
EaeI YGGCCR 1 cut(s) 221
EciI GGCGGA 1 cut(s) 507
Eco31I GGTCTC 1 cut(s) 206
Eco47I GGWCC 1 cut(s) 431
Eco81I CCTNAGG 1 cut(s) 348
FaeI CATG 2 cut(s) 97, 454
FaiI YATR 8 cut(s) 95, 122, 184, 215, 392, 452, 485, 511
FatI CATG 2 cut(s) 93, 450
Fnu4HI GCNGC 2 cut(s) 48, 233
Fsp4HI GCNGC 2 cut(s) 48, 233
GlaI GCGC 3 cut(s) 6, 40, 231
GluI GCNGC 2 cut(s) 48, 233
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 3 cut(s) 223, 398, 449
HhaI GCGC 3 cut(s) 7, 41, 232
Hin1II CATG 2 cut(s) 97, 454
Hin6I GCGC 3 cut(s) 5, 39, 230
HinP1I GCGC 3 cut(s) 5, 39, 230
HinfI GANTC 2 cut(s) 82, 242
HphI GGTGA 1 cut(s) 306
Hpy188I TCNGA 2 cut(s) 81, 258
Hpy188III TCNNGA 1 cut(s) 340
HpyAV CCTTC 1 cut(s) 289
HpyCH4V TGCA 2 cut(s) 47, 274
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 229
HpyF3I CTNAG 2 cut(s) 291, 348
Hsp92II CATG 2 cut(s) 97, 454
HspAI GCGC 3 cut(s) 5, 39, 230
LmnI GCTCC 2 cut(s) 104, 380
LpnPI CCDG 8 cut(s) 111, 155, 205, 231, 330, 360, 414, 474
Lsp1109I GCAGC 2 cut(s) 59, 244
MaeIII GTNAC 2 cut(s) 301, 312
MhlI GDGCHC 1 cut(s) 109
MlsI TGGCCA 1 cut(s) 223
MluCI AATT 6 cut(s) 168, 209, 353, 357, 375, 438
MluNI TGGCCA 1 cut(s) 223
MlyI GAGTC 1 cut(s) 76
MmeI TCCRAC 1 cut(s) 82
MnlI CCTC 9 cut(s) 10, 21, 75, 96, 142, 191, 343, 436, 439
Mox20I TGGCCA 1 cut(s) 223
MroXI GAANNNNTTC 1 cut(s) 357
MscI TGGCCA 1 cut(s) 223
MseI TTAA 1 cut(s) 356
Msp20I TGGCCA 1 cut(s) 223
MvnI CGCG 1 cut(s) 39
MwoI GCNNNNNNNGC 2 cut(s) 47, 229
NlaIII CATG 2 cut(s) 97, 454
NmuCI GTSAC 1 cut(s) 312
PcsI WCGNNNNNNNCGW 1 cut(s) 463
PdmI GAANNNNTTC 1 cut(s) 357
PfeI GAWTC 1 cut(s) 242
PflMI CCANNNNNTGG 1 cut(s) 323
PkrI GCNGC 2 cut(s) 49, 234
PleI GAGTC 1 cut(s) 76
PpsI GAGTC 1 cut(s) 76
PshBI ATTAAT 1 cut(s) 356
PsiI TTATAA 1 cut(s) 392
PspPI GGNCC 2 cut(s) 397, 431
RsaI GTAC 1 cut(s) 460
RsaNI GTAC 1 cut(s) 459
SaqAI TTAA 1 cut(s) 356
SatI GCNGC 2 cut(s) 48, 233
Sau96I GGNCC 2 cut(s) 397, 431
SchI GAGTC 1 cut(s) 76
SduI GDGCHC 1 cut(s) 109
SetI ASST 8 cut(s) 21, 32, 52, 147, 202, 237, 283, 349
SinI GGWCC 1 cut(s) 431
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
Sse9I AATT 6 cut(s) 168, 209, 353, 357, 375, 438
SsiI CCGC 1 cut(s) 492
TaqI TCGA 2 cut(s) 15, 321
TasI AATT 6 cut(s) 168, 209, 353, 357, 375, 438
TfiI GAWTC 1 cut(s) 242
Tru1I TTAA 1 cut(s) 356
Tru9I TTAA 1 cut(s) 356
TscAI CASTG 2 cut(s) 295, 433
TseFI GTSAC 1 cut(s) 312
TseI GCWGC 2 cut(s) 47, 232
Tsp45I GTSAC 1 cut(s) 312
TspDTI ATGAA 2 cut(s) 82, 230
TspRI CASTG 2 cut(s) 295, 433
Van91I CCANNNNNTGG 1 cut(s) 323
VpaK11BI GGWCC 1 cut(s) 431
VspI ATTAAT 1 cut(s) 356
XapI RAATTY 1 cut(s) 209
XcmI CCANNNNNNNNNTGG 2 cut(s) 170, 374
XmnI GAANNNNTTC 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.