Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_5g36560 |
| rosa_chinensis | RchiOBHm_Chr7g0242231 |
| rosa_multiflora | Rmu_sc0000429.1_g000027 |
| rosa_samantha | Rh1DG069500 |
| rosa_wichuraiana | Rw0G009470 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 191 |
| AdeI | CACNNNGTG | 1 cut(s) | 218 |
| AgsI | TTSAA | 2 cut(s) | 34, 148 |
| AluBI | AGCT | 2 cut(s) | 50, 55 |
| AluI | AGCT | 2 cut(s) | 50, 55 |
| Alw21I | GWGCWC | 1 cut(s) | 43 |
| Alw26I | GTCTC | 2 cut(s) | 178, 227 |
| AlwI | GGATC | 1 cut(s) | 191 |
| ApeKI | GCWGC | 1 cut(s) | 47 |
| AspLEI | GCGC | 1 cut(s) | 7 |
| AsuHPI | GGTGA | 1 cut(s) | 109 |
| AsuII | TTCGAA | 1 cut(s) | 126 |
| BauI | CACGAG | 1 cut(s) | 42 |
| Bbv12I | GWGCWC | 1 cut(s) | 43 |
| BbvI | GCAGC | 1 cut(s) | 59 |
| BcoDI | GTCTC | 2 cut(s) | 178, 227 |
| BfaI | CTAG | 1 cut(s) | 194 |
| BfoI | RGCGCY | 1 cut(s) | 8 |
| BisI | GCNGC | 1 cut(s) | 48 |
| BlpI | GCTNAGC | 1 cut(s) | 51 |
| BlsI | GCNGC | 1 cut(s) | 49 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 134, 166 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 51 |
| Bpu14I | TTCGAA | 1 cut(s) | 126 |
| BsaI | GGTCTC | 1 cut(s) | 227 |
| BseMII | CTCAG | 1 cut(s) | 65 |
| BseRI | GAGGAG | 1 cut(s) | 75 |
| BseXI | GCAGC | 1 cut(s) | 59 |
| BsgI | GTGCAG | 1 cut(s) | 66 |
| BsiHKAI | GWGCWC | 1 cut(s) | 43 |
| BsiSI | CCGG | 1 cut(s) | 153 |
| BsmAI | GTCTC | 2 cut(s) | 178, 227 |
| BsmBI | CGTCTC | 1 cut(s) | 178 |
| Bso31I | GGTCTC | 1 cut(s) | 227 |
| Bsp119I | TTCGAA | 1 cut(s) | 126 |
| Bsp1286I | GDGCHC | 1 cut(s) | 43 |
| Bsp143I | GATC | 2 cut(s) | 133, 196 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 51 |
| BspCNI | CTCAG | 1 cut(s) | 64 |
| BspPI | GGATC | 1 cut(s) | 191 |
| BspT104I | TTCGAA | 1 cut(s) | 126 |
| BspTNI | GGTCTC | 1 cut(s) | 227 |
| BssMI | GATC | 2 cut(s) | 133, 196 |
| BssSI | CACGAG | 1 cut(s) | 42 |
| Bst2BI | CACGAG | 1 cut(s) | 42 |
| Bst4CI | ACNGT | 1 cut(s) | 218 |
| BstBI | TTCGAA | 1 cut(s) | 126 |
| BstC8I | GCNNGC | 1 cut(s) | 186 |
| BstDEI | CTNAG | 1 cut(s) | 51 |
| BstH2I | RGCGCY | 1 cut(s) | 8 |
| BstHHI | GCGC | 1 cut(s) | 7 |
| BstKTI | GATC | 2 cut(s) | 136, 199 |
| BstMAI | GTCTC | 2 cut(s) | 178, 227 |
| BstMBI | GATC | 2 cut(s) | 133, 196 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 47 |
| BstV1I | GCAGC | 1 cut(s) | 59 |
| BstX2I | RGATCY | 1 cut(s) | 196 |
| BstYI | RGATCY | 1 cut(s) | 196 |
| BtsIMutI | CAGTG | 1 cut(s) | 214 |
| Cac8I | GCNNGC | 1 cut(s) | 186 |
| CfoI | GCGC | 1 cut(s) | 7 |
| CviJI | RGCY | 3 cut(s) | 50, 55, 114 |
| CviKI_1 | RGCY | 3 cut(s) | 50, 55, 114 |
| DdeI | CTNAG | 1 cut(s) | 51 |
| DpnI | GATC | 2 cut(s) | 135, 198 |
| DpnII | GATC | 2 cut(s) | 133, 196 |
| DraIII | CACNNNGTG | 1 cut(s) | 218 |
| Eco31I | GGTCTC | 1 cut(s) | 227 |
| Esp3I | CGTCTC | 1 cut(s) | 178 |
| FaiI | YATR | 1 cut(s) | 76 |
| Fnu4HI | GCNGC | 1 cut(s) | 48 |
| Fsp4HI | GCNGC | 1 cut(s) | 48 |
| FspBI | CTAG | 1 cut(s) | 194 |
| GlaI | GCGC | 1 cut(s) | 6 |
| GluI | GCNGC | 1 cut(s) | 48 |
| HaeII | RGCGCY | 1 cut(s) | 8 |
| HapII | CCGG | 1 cut(s) | 153 |
| HhaI | GCGC | 1 cut(s) | 7 |
| Hin6I | GCGC | 1 cut(s) | 5 |
| HinP1I | GCGC | 1 cut(s) | 5 |
| HinfI | GANTC | 1 cut(s) | 82 |
| HpaII | CCGG | 1 cut(s) | 153 |
| HphI | GGTGA | 1 cut(s) | 109 |
| Hpy188I | TCNGA | 1 cut(s) | 81 |
| HpyAV | CCTTC | 1 cut(s) | 172 |
| HpyCH4III | ACNGT | 1 cut(s) | 218 |
| HpyCH4V | TGCA | 1 cut(s) | 47 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 47 |
| HpyF3I | CTNAG | 1 cut(s) | 51 |
| HspAI | GCGC | 1 cut(s) | 5 |
| Kzo9I | GATC | 2 cut(s) | 133, 196 |
| LpnPI | CCDG | 2 cut(s) | 111, 166 |
| Lsp1109I | GCAGC | 1 cut(s) | 59 |
| MaeI | CTAG | 1 cut(s) | 194 |
| MalI | GATC | 2 cut(s) | 135, 198 |
| MboI | GATC | 2 cut(s) | 133, 196 |
| MboII | GAAGA | 2 cut(s) | 157, 160 |
| MflI | RGATCY | 1 cut(s) | 196 |
| MhlI | GDGCHC | 1 cut(s) | 43 |
| MluCI | AATT | 2 cut(s) | 68, 138 |
| MlyI | GAGTC | 1 cut(s) | 76 |
| MmeI | TCCRAC | 2 cut(s) | 82, 224 |
| MnlI | CCTC | 4 cut(s) | 10, 21, 75, 96 |
| MseI | TTAA | 1 cut(s) | 209 |
| MspA1I | CMGCKG | 1 cut(s) | 55 |
| MspI | CCGG | 1 cut(s) | 153 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 47 |
| NdeII | GATC | 2 cut(s) | 133, 196 |
| NspV | TTCGAA | 1 cut(s) | 126 |
| PkrI | GCNGC | 1 cut(s) | 49 |
| PleI | GAGTC | 1 cut(s) | 76 |
| PpsI | GAGTC | 1 cut(s) | 76 |
| PsuI | RGATCY | 1 cut(s) | 196 |
| PvuII | CAGCTG | 1 cut(s) | 55 |
| SaqAI | TTAA | 1 cut(s) | 209 |
| SatI | GCNGC | 1 cut(s) | 48 |
| Sau3AI | GATC | 2 cut(s) | 133, 196 |
| SchI | GAGTC | 1 cut(s) | 76 |
| SduI | GDGCHC | 1 cut(s) | 43 |
| SetI | ASST | 4 cut(s) | 21, 32, 52, 57 |
| SfuI | TTCGAA | 1 cut(s) | 126 |
| SgeI | CNNG | 9 cut(s) | 28, 50, 54, 56, 110, 122, 165, 197, 206 |
| Sse9I | AATT | 2 cut(s) | 68, 138 |
| SspMI | CTAG | 1 cut(s) | 194 |
| TaaI | ACNGT | 1 cut(s) | 218 |
| TaqI | TCGA | 3 cut(s) | 15, 126, 136 |
| TasI | AATT | 2 cut(s) | 68, 138 |
| Tru1I | TTAA | 1 cut(s) | 209 |
| Tru9I | TTAA | 1 cut(s) | 209 |
| TscAI | CASTG | 1 cut(s) | 221 |
| TseI | GCWGC | 1 cut(s) | 47 |
| TspDTI | ATGAA | 1 cut(s) | 82 |
| TspRI | CASTG | 1 cut(s) | 221 |
| XspI | CTAG | 1 cut(s) | 194 |