FvH4_5g36560

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004542
Physical Location & Seq
Forward (+)
26810035 .. 26816393
6359 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g36560.t3

Sequence Viewer

Length: 1203 bp
ATGGCGCTTCGTTTCGAGGTTTTGGCGAGGTTTAATCGCGCTCGCGCAGCTCAACTGACGCTCCCTCACTTTGTATGCCAGACTCCTCTTTTTATGCCTGTTGGGACACAAGGAACTATAAAGGGGTTGGCAACTGACCAACTTGAGGAGATTGGTTGCCAAATAATTCTAGGGAATACATATCATTTGGCACTTCGTCCTACCTCTGAGCTTATTGACGACATGGGAGGCCTCCACAAATTTATGAACTGGCCAAGAGCGATGCTTACCGATTCTGGTGGCTTTCAGATGGTATCCTTATTGCATTTAGCTGACATCACTGAGAAGGGTGTAACATTTCAGTCACCGGTCGATGGAAAGCCAATGCTCTTGACACCTGAGGAATCAATTCAAATCCAAAACAGAATCGGAGCAGATATTATAATGGCCCTTGATGATGTAGTGAAAACCACCATCACTGGTCCAAGAATCGAGGAGGCCATGTATCGTACTCTACGATGGATTGACAGATGTATAGCAGCTCATACAAGACCAAATGAACAGAACTTATTTGGTATTGTTCAAGGTGGCTTAGATCCTGTACTGAGGGATATATGCGTCAGAGGCTTGGTAGAAAGGAATTTGCCTGGCTATGCCATAGGCGGTCTTGCTGGTGGCGAAAGTAAGGATTCATTTTGGCGTGTTGTTGCTCAGTGCACTGCTGCACTGCCTGAGGACAAGCCGCGATATGTTATGGGAGTTGGTTATCCATTGGATATTGTAGTTTGCAGTGCTTTAGGTGCCGACATGTATGATTGCGTTTATCCAACACGTACTGCTCGCTTTGGCACAGCTTTGATACCTGAGGGAGTGCTGAAGCTGAAGCACAAAGCTATGGCTAAGGATACACGCCCTATCGATCCTACATGTGCTTGCATGGTATGCAAGAAATATACTAGAGCTTACATTCATTGCCTAGTTACAAAAGATGCTATGGGATCTCAGCTTCTGTCATATCACAATCTGTATTACATGCTGAAGCTAAGTAGAGATCTACACTCGTCAATAATCCAAGGAACATTTCCAGAGTTTGTATGCAACTTTCTGCAAACAATGTTTCCTAAAGGCGATGTCCCGGAATGGGTCTGCAATGCCATGGAGGTTGCGGGCATTGACATCTCTTCCTGTTGTACTCCATTTTCTTCATCCAAAGATTCGGATTAG

Protein Analysis

401

Amino Acids

44.26

Weight (kDa)

6.32

Isoelectric Point (pI)

39.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 13 - 358 4e-151 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 422
AccB1I GGYRCC 1 cut(s) 779
AccII CGCG 3 cut(s) 39, 45, 724
AciI CCGC 3 cut(s) 642, 722, 1145
AclWI GGATC 3 cut(s) 569, 893, 985
AcoI YGGCCR 1 cut(s) 251
AcsI RAATTY 2 cut(s) 239, 619
AcuI CTGAAG 3 cut(s) 875, 881, 1037
AfaI GTAC 4 cut(s) 490, 582, 814, 1171
AfiI CCNNNNNNNGG 3 cut(s) 145, 353, 1120
AflIII ACRYGT 3 cut(s) 786, 809, 905
AgeI ACCGGT 1 cut(s) 346
AgsI TTSAA 2 cut(s) 392, 563
AjnI CCWGG 1 cut(s) 625
AjuI GAANNNNNNNTTGG 2 cut(s) 1044, 1076
Alw21I GWGCWC 1 cut(s) 698
Alw44I GTGCAC 1 cut(s) 694
AlwI GGATC 3 cut(s) 569, 893, 985
AlwNI CAGNNNCTG 1 cut(s) 988
AoxI GGCC 4 cut(s) 229, 251, 426, 477
ApaLI GTGCAC 1 cut(s) 694
ApeKI GCWGC 3 cut(s) 47, 518, 701
ApoI RAATTY 2 cut(s) 239, 619
AsiGI ACCGGT 1 cut(s) 346
Asp700I GAANNNNTTC 1 cut(s) 387
AspLEI GCGC 3 cut(s) 7, 41, 47
AspS9I GGNCC 2 cut(s) 427, 461
AsuC2I CCSGG 1 cut(s) 1115
AsuHPI GGTGA 1 cut(s) 336
AvaII GGWCC 1 cut(s) 461
AxyI CCTNAGG 3 cut(s) 378, 711, 843
BaeGI GKGCMC 1 cut(s) 698
BalI TGGCCA 1 cut(s) 253
BanI GGYRCC 1 cut(s) 779
Bbv12I GWGCWC 1 cut(s) 698
BbvI GCAGC 3 cut(s) 59, 530, 688
BccI CCATC 4 cut(s) 283, 347, 461, 492
BciT130I CCWGG 1 cut(s) 627
BciVI GTATCC 2 cut(s) 304, 877
BcnI CCSGG 1 cut(s) 1115
BfaI CTAG 3 cut(s) 170, 936, 956
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 2 cut(s) 304, 877
BglII AGATCT 1 cut(s) 1030
BisI GCNGC 4 cut(s) 48, 519, 702, 722
BlsI GCNGC 4 cut(s) 49, 520, 703, 723
Bme1390I CCNGG 2 cut(s) 627, 1115
Bme18I GGWCC 1 cut(s) 461
BmgT120I GGNCC 2 cut(s) 427, 461
BmiI GGNNCC 1 cut(s) 781
BmrFI CCNGG 2 cut(s) 627, 1115
BmsI GCATC 2 cut(s) 252, 958
Bpu10I CCTNAGC 1 cut(s) 879
BpuEI CTTGAG 1 cut(s) 164
BpuMI CCSGG 1 cut(s) 1115
Bsa29I ATCGAT 1 cut(s) 897
BsaAI YACGTR 1 cut(s) 812
BsaJI CCNNGG 2 cut(s) 1051, 1134
BsaWI WCCGGW 1 cut(s) 346
BsaXI ACNNNNNCTCC 4 cut(s) 45, 75, 467, 497
Bsc4I CCNNNNNNNGG 3 cut(s) 145, 353, 1120
Bse118I RCCGGY 1 cut(s) 346
Bse1I ACTGG 2 cut(s) 254, 463
Bse21I CCTNAGG 3 cut(s) 378, 711, 843
Bse3DI GCAATG 2 cut(s) 949, 1135
BseBI CCWGG 1 cut(s) 627
BseCI ATCGAT 1 cut(s) 897
BseDI CCNNGG 2 cut(s) 1051, 1134
BseGI GGATG 1 cut(s) 1184
BseLI CCNNNNNNNGG 3 cut(s) 145, 353, 1120
BseMI GCAATG 2 cut(s) 949, 1135
BseMII CTCAG 8 cut(s) 198, 312, 369, 575, 702, 704, 834, 995
BseNI ACTGG 2 cut(s) 254, 463
BseRI GAGGAG 3 cut(s) 75, 161, 488
BseSI GKGCMC 1 cut(s) 698
BseXI GCAGC 3 cut(s) 59, 530, 688
BsgI GTGCAG 1 cut(s) 687
Bsh1236I CGCG 3 cut(s) 39, 45, 724
Bsh1285I CGRYCG 1 cut(s) 351
BshFI GGCC 4 cut(s) 231, 253, 428, 479
BshNI GGYRCC 1 cut(s) 779
BshTI ACCGGT 1 cut(s) 346
BshVI ATCGAT 1 cut(s) 897
BsiEI CGRYCG 1 cut(s) 351
BsiHKAI GWGCWC 1 cut(s) 698
BsiSI CCGG 2 cut(s) 347, 1115
BslFI GGGAC 2 cut(s) 118, 1097
BslI CCNNNNNNNGG 3 cut(s) 145, 353, 1120
BsmFI GGGAC 2 cut(s) 118, 1097
BsnI GGCC 4 cut(s) 231, 253, 428, 479
Bsp1286I GDGCHC 1 cut(s) 698
Bsp143I GATC 4 cut(s) 574, 898, 977, 1030
Bsp19I CCATGG 1 cut(s) 1134
BspACI CCGC 3 cut(s) 642, 722, 1145
BspANI GGCC 4 cut(s) 231, 253, 428, 479
BspCNI CTCAG 8 cut(s) 199, 313, 370, 576, 703, 703, 835, 994
BspDI ATCGAT 1 cut(s) 897
BspFNI CGCG 3 cut(s) 39, 45, 724
BspLI GGNNCC 1 cut(s) 781
BspPI GGATC 3 cut(s) 569, 893, 985
BspT107I GGYRCC 1 cut(s) 779
BsrDI GCAATG 2 cut(s) 949, 1135
BsrFI RCCGGY 1 cut(s) 346
BsrI ACTGG 2 cut(s) 254, 463
BssAI RCCGGY 1 cut(s) 346
BssECI CCNNGG 2 cut(s) 1051, 1134
BssMI GATC 4 cut(s) 574, 898, 977, 1030
BssT1I CCWWGG 2 cut(s) 1051, 1134
Bst2UI CCWGG 1 cut(s) 627
Bst6I CTCTTC 1 cut(s) 1165
BstAPI GCANNNNNTGC 1 cut(s) 921
BstBAI YACGTR 1 cut(s) 812
BstC8I GCNNGC 4 cut(s) 43, 820, 913, 1147
BstDSI CCRYGG 1 cut(s) 1134
BstF5I GGATG 1 cut(s) 1184
BstFNI CGCG 3 cut(s) 39, 45, 724
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 41, 47
BstKTI GATC 4 cut(s) 577, 901, 980, 1033
BstMBI GATC 4 cut(s) 574, 898, 977, 1030
BstMCI CGRYCG 1 cut(s) 351
BstMWI GCNNNNNNNGC 4 cut(s) 47, 603, 779, 921
BstNI CCWGG 1 cut(s) 627
BstNSI RCATGY 3 cut(s) 790, 909, 1015
BstSCI CCNGG 2 cut(s) 625, 1113
BstSLI GKGCMC 1 cut(s) 698
BstUI CGCG 3 cut(s) 39, 45, 724
BstV1I GCAGC 3 cut(s) 59, 530, 688
BstX2I RGATCY 3 cut(s) 574, 977, 1030
BstYI RGATCY 3 cut(s) 574, 977, 1030
Bsu15I ATCGAT 1 cut(s) 897
Bsu36I CCTNAGG 3 cut(s) 378, 711, 843
BsuI GTATCC 2 cut(s) 304, 877
BsuRI GGCC 4 cut(s) 231, 253, 428, 479
BsuTUI ATCGAT 1 cut(s) 897
BtgI CCRYGG 1 cut(s) 1134
BtgZI GCGATG 2 cut(s) 275, 1122
BtsCI GGATG 1 cut(s) 1184
BtsI GCAGTG 3 cut(s) 696, 704, 775
BtsIMutI CAGTG 6 cut(s) 318, 456, 696, 698, 704, 775
Cac8I GCNNGC 4 cut(s) 43, 820, 913, 1147
CaiI CAGNNNCTG 1 cut(s) 988
CfoI GCGC 3 cut(s) 7, 41, 47
Cfr10I RCCGGY 1 cut(s) 346
Cfr13I GGNCC 2 cut(s) 427, 461
ClaI ATCGAT 1 cut(s) 897
CseI GACGC 2 cut(s) 67, 586
Csp6I GTAC 4 cut(s) 489, 581, 813, 1170
CspAI ACCGGT 1 cut(s) 346
CviAII CATG 7 cut(s) 223, 481, 787, 906, 916, 1012, 1135
CviQI GTAC 4 cut(s) 489, 581, 813, 1170
DpnI GATC 4 cut(s) 576, 900, 979, 1032
DpnII GATC 4 cut(s) 574, 898, 977, 1030
EaeI YGGCCR 1 cut(s) 251
Eam1104I CTCTTC 1 cut(s) 1165
EarI CTCTTC 1 cut(s) 1165
Eco130I CCWWGG 2 cut(s) 1051, 1134
Eco147I AGGCCT 1 cut(s) 231
Eco47I GGWCC 1 cut(s) 461
Eco57I CTGAAG 3 cut(s) 875, 881, 1037
Eco81I CCTNAGG 3 cut(s) 378, 711, 843
EcoRII CCWGG 1 cut(s) 625
EcoT14I CCWWGG 2 cut(s) 1051, 1134
ErhI CCWWGG 2 cut(s) 1051, 1134
FaeI CATG 7 cut(s) 226, 484, 790, 909, 919, 1015, 1138
FaqI GGGAC 2 cut(s) 118, 1097
FatI CATG 7 cut(s) 222, 480, 786, 905, 915, 1011, 1134
FauI CCCGC 1 cut(s) 1138
Fnu4HI GCNGC 4 cut(s) 48, 519, 702, 722
FokI GGATG 1 cut(s) 1171
Fsp4HI GCNGC 4 cut(s) 48, 519, 702, 722
FspBI CTAG 3 cut(s) 170, 936, 956
GlaI GCGC 3 cut(s) 6, 40, 46
GluI GCNGC 4 cut(s) 48, 519, 702, 722
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 4 cut(s) 231, 253, 428, 479
HapII CCGG 2 cut(s) 347, 1115
HgaI GACGC 2 cut(s) 67, 586
HhaI GCGC 3 cut(s) 7, 41, 47
Hin1II CATG 7 cut(s) 226, 484, 790, 909, 919, 1015, 1138
Hin6I GCGC 3 cut(s) 5, 39, 45
HinP1I GCGC 3 cut(s) 5, 39, 45
HinfI GANTC 7 cut(s) 82, 272, 383, 405, 468, 668, 1193
HpaII CCGG 2 cut(s) 347, 1115
HphI GGTGA 1 cut(s) 336
Hpy166II GTNNAC 1 cut(s) 696
Hpy188I TCNGA 5 cut(s) 208, 288, 410, 602, 1198
Hpy188III TCNNGA 2 cut(s) 370, 1064
Hpy8I GTNNAC 1 cut(s) 696
HpyAV CCTTC 1 cut(s) 319
HpyCH4IV ACGT 1 cut(s) 811
HpyCH4V TGCA 9 cut(s) 304, 696, 704, 768, 915, 924, 1077, 1087, 1128
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 603, 779, 921
HpySE526I ACGT 1 cut(s) 811
Hsp92II CATG 7 cut(s) 226, 484, 790, 909, 919, 1015, 1138
HspAI GCGC 3 cut(s) 5, 39, 45
Kzo9I GATC 4 cut(s) 574, 898, 977, 1030
LmnI GCTCC 2 cut(s) 66, 410
Lsp1109I GCAGC 3 cut(s) 59, 530, 688
LweI GCATC 2 cut(s) 252, 958
MaeI CTAG 3 cut(s) 170, 936, 956
MaeII ACGT 1 cut(s) 811
MaeIII GTNAC 3 cut(s) 331, 342, 958
MalI GATC 4 cut(s) 576, 900, 979, 1032
MboI GATC 4 cut(s) 574, 898, 977, 1030
MboII GAAGA 2 cut(s) 1152, 1173
MflI RGATCY 3 cut(s) 574, 977, 1030
MhlI GDGCHC 1 cut(s) 698
MlsI TGGCCA 1 cut(s) 253
MluCI AATT 4 cut(s) 165, 239, 387, 619
MluNI TGGCCA 1 cut(s) 253
MlyI GAGTC 1 cut(s) 76
MmeI TCCRAC 1 cut(s) 830
Mox20I TGGCCA 1 cut(s) 253
MroXI GAANNNNTTC 1 cut(s) 387
MscI TGGCCA 1 cut(s) 253
MseI TTAA 1 cut(s) 33
Msp20I TGGCCA 1 cut(s) 253
MspI CCGG 2 cut(s) 347, 1115
MspR9I CCNGG 2 cut(s) 627, 1115
MvaI CCWGG 1 cut(s) 627
MvnI CGCG 3 cut(s) 39, 45, 724
MwoI GCNNNNNNNGC 4 cut(s) 47, 603, 779, 921
NciI CCSGG 1 cut(s) 1115
NcoI CCATGG 1 cut(s) 1134
NdeII GATC 4 cut(s) 574, 898, 977, 1030
NlaIII CATG 7 cut(s) 226, 484, 790, 909, 919, 1015, 1138
NlaIV GGNNCC 1 cut(s) 781
NmuCI GTSAC 1 cut(s) 342
NspI RCATGY 3 cut(s) 790, 909, 1015
PceI AGGCCT 1 cut(s) 231
PciI ACATGT 2 cut(s) 786, 905
PcsI WCGNNNNNNNCGW 1 cut(s) 493
PdmI GAANNNNTTC 1 cut(s) 387
PfeI GAWTC 6 cut(s) 272, 383, 405, 468, 668, 1193
PfoI TCCNGGA 1 cut(s) 1113
PinAI ACCGGT 1 cut(s) 346
PkrI GCNGC 4 cut(s) 49, 520, 703, 723
PleI GAGTC 1 cut(s) 76
PpsI GAGTC 1 cut(s) 76
Ppu21I YACGTR 1 cut(s) 812
PscI ACATGT 2 cut(s) 786, 905
PsiI TTATAA 1 cut(s) 422
Psp6I CCWGG 1 cut(s) 625
PspGI CCWGG 1 cut(s) 625
PspN4I GGNNCC 1 cut(s) 781
PspPI GGNCC 2 cut(s) 427, 461
PstNI CAGNNNCTG 1 cut(s) 988
PsuI RGATCY 3 cut(s) 574, 977, 1030
RsaI GTAC 4 cut(s) 490, 582, 814, 1171
RsaNI GTAC 4 cut(s) 489, 581, 813, 1170
SaqAI TTAA 1 cut(s) 33
SatI GCNGC 4 cut(s) 48, 519, 702, 722
Sau3AI GATC 4 cut(s) 574, 898, 977, 1030
Sau96I GGNCC 2 cut(s) 427, 461
SchI GAGTC 1 cut(s) 76
ScrFI CCNGG 2 cut(s) 627, 1115
SduI GDGCHC 1 cut(s) 698
SfaNI GCATC 2 cut(s) 252, 958
SinI GGWCC 1 cut(s) 461
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
Sse9I AATT 4 cut(s) 165, 239, 387, 619
SseBI AGGCCT 1 cut(s) 231
SsiI CCGC 3 cut(s) 642, 722, 1145
SspMI CTAG 3 cut(s) 170, 936, 956
StuI AGGCCT 1 cut(s) 231
StyD4I CCNGG 2 cut(s) 625, 1113
StyI CCWWGG 2 cut(s) 1051, 1134
TaiI ACGT 1 cut(s) 814
TaqI TCGA 4 cut(s) 15, 351, 471, 897
TasI AATT 4 cut(s) 165, 239, 387, 619
TatI WGTACW 2 cut(s) 580, 1169
TauI GCSGC 1 cut(s) 724
TfiI GAWTC 6 cut(s) 272, 383, 405, 468, 668, 1193
Tru1I TTAA 1 cut(s) 33
Tru9I TTAA 1 cut(s) 33
TscAI CASTG 6 cut(s) 325, 463, 698, 703, 711, 775
TseFI GTSAC 1 cut(s) 342
TseI GCWGC 3 cut(s) 47, 518, 701
Tsp45I GTSAC 1 cut(s) 342
TspDTI ATGAA 5 cut(s) 260, 552, 660, 938, 1173
TspRI CASTG 6 cut(s) 325, 463, 698, 703, 711, 775
VneI GTGCAC 1 cut(s) 694
VpaK11BI GGWCC 1 cut(s) 461
XapI RAATTY 2 cut(s) 239, 619
XceI RCATGY 3 cut(s) 790, 909, 1015
XmnI GAANNNNTTC 1 cut(s) 387
XspI CTAG 3 cut(s) 170, 936, 956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.