RchiOBHm_Chr7g0242241

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
Physical Location & Seq
Forward (+)
68396766 .. 68399928
3163 bp
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UTR
Exon/CDS
Intron
PRQ21711

Sequence Viewer

Length: 414 bp
ATGGAATCATGTGTGTCCTTATTGCATTTAGCTGACATCACTGAGAAGGGTGTAACATTTCAGTCACCAGTCGATGGAAAGCCAATGCTATTGACACCTGAGGAATTAATTCAAATCCAAAATAGAATTGGAGCAGATATTATAATGGCCCTTGATGATGTAGTGAAAACCACCATCACTGGTCCAAGAATTGAGGAGGCCATGTATCGTATTCTACGATGGATTGACAAATGTATAGCAGCTCATACAAGACCGAGCGAGCAGAACTTATTTGGTATTGTTCAAGGTGGCTTAGATCCTGTACTAAGGGATATATGCGTCAGAGGCTTAGTAGAATGGAATTTGCCTGTCTATGCCATAGGCGGTCTTGCGGATGGCGAAAGTAAAGATTCATTTCGGCGCTGCACTGCCTGA

Protein Analysis

137

Amino Acids

15.15

Weight (kDa)

4.97

Isoelectric Point (pI)

51.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 4 - 134 1.3e-42 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 143
AccB7I CCANNNNNTGG 1 cut(s) 74
AciI CCGC 2 cut(s) 363, 371
AclWI GGATC 1 cut(s) 290
AcsI RAATTY 1 cut(s) 340
AfaI GTAC 1 cut(s) 303
AfiI CCNNNNNNNGG 1 cut(s) 74
AgsI TTSAA 2 cut(s) 113, 284
AluBI AGCT 2 cut(s) 32, 242
AluI AGCT 2 cut(s) 32, 242
AlwI GGATC 1 cut(s) 290
AoxI GGCC 2 cut(s) 147, 198
ApeKI GCWGC 2 cut(s) 239, 402
ApoI RAATTY 1 cut(s) 340
AseI ATTAAT 1 cut(s) 107
Asp700I GAANNNNTTC 1 cut(s) 108
AspLEI GCGC 1 cut(s) 402
AspS9I GGNCC 2 cut(s) 148, 182
AsuHPI GGTGA 1 cut(s) 57
AvaII GGWCC 1 cut(s) 182
AxyI CCTNAGG 1 cut(s) 99
BbvI GCAGC 2 cut(s) 251, 389
BccI CCATC 4 cut(s) 68, 182, 213, 368
BfoI RGCGCY 1 cut(s) 403
BisI GCNGC 2 cut(s) 240, 403
BlsI GCNGC 2 cut(s) 241, 404
Bme18I GGWCC 1 cut(s) 182
BmgT120I GGNCC 2 cut(s) 148, 182
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bsc4I CCNNNNNNNGG 1 cut(s) 74
Bse1I ACTGG 2 cut(s) 68, 184
Bse21I CCTNAGG 1 cut(s) 99
BseGI GGATG 1 cut(s) 379
BseLI CCNNNNNNNGG 1 cut(s) 74
BseMII CTCAG 2 cut(s) 33, 90
BseNI ACTGG 2 cut(s) 68, 184
BseRI GAGGAG 1 cut(s) 209
BseXI GCAGC 2 cut(s) 251, 389
BsgI GTGCAG 1 cut(s) 388
BshFI GGCC 2 cut(s) 149, 200
BslI CCNNNNNNNGG 1 cut(s) 74
BsnI GGCC 2 cut(s) 149, 200
Bsp143I GATC 1 cut(s) 295
BspACI CCGC 2 cut(s) 363, 371
BspANI GGCC 2 cut(s) 149, 200
BspCNI CTCAG 2 cut(s) 34, 91
BspPI GGATC 1 cut(s) 290
BsrI ACTGG 2 cut(s) 68, 184
BssMI GATC 1 cut(s) 295
BstC8I GCNNGC 1 cut(s) 260
BstDEI CTNAG 5 cut(s) 42, 99, 292, 305, 328
BstF5I GGATG 1 cut(s) 379
BstH2I RGCGCY 1 cut(s) 403
BstHHI GCGC 1 cut(s) 402
BstKTI GATC 1 cut(s) 298
BstMBI GATC 1 cut(s) 295
BstMWI GCNNNNNNNGC 1 cut(s) 324
BstV1I GCAGC 2 cut(s) 251, 389
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
Bsu36I CCTNAGG 1 cut(s) 99
BsuRI GGCC 2 cut(s) 149, 200
BtsCI GGATG 1 cut(s) 379
BtsI GCAGTG 1 cut(s) 405
BtsIMutI CAGTG 3 cut(s) 39, 177, 405
Cac8I GCNNGC 1 cut(s) 260
CfoI GCGC 1 cut(s) 402
Cfr13I GGNCC 2 cut(s) 148, 182
CseI GACGC 1 cut(s) 307
Csp6I GTAC 1 cut(s) 302
CviAII CATG 2 cut(s) 9, 202
CviJI RGCY 7 cut(s) 32, 82, 149, 200, 242, 291, 327
CviKI_1 RGCY 7 cut(s) 32, 82, 149, 200, 242, 291, 327
CviQI GTAC 1 cut(s) 302
DdeI CTNAG 5 cut(s) 42, 99, 292, 305, 328
DpnI GATC 1 cut(s) 297
DpnII GATC 1 cut(s) 295
Eco47I GGWCC 1 cut(s) 182
Eco81I CCTNAGG 1 cut(s) 99
FaeI CATG 2 cut(s) 12, 205
FaiI YATR 9 cut(s) 10, 143, 203, 236, 246, 314, 316, 354, 359
FatI CATG 2 cut(s) 8, 201
Fnu4HI GCNGC 2 cut(s) 240, 403
FokI GGATG 1 cut(s) 386
Fsp4HI GCNGC 2 cut(s) 240, 403
GlaI GCGC 1 cut(s) 401
GluI GCNGC 2 cut(s) 240, 403
HaeII RGCGCY 1 cut(s) 403
HaeIII GGCC 2 cut(s) 149, 200
HgaI GACGC 1 cut(s) 307
HhaI GCGC 1 cut(s) 402
Hin1II CATG 2 cut(s) 12, 205
Hin6I GCGC 1 cut(s) 400
HinP1I GCGC 1 cut(s) 400
HinfI GANTC 2 cut(s) 5, 389
HphI GGTGA 1 cut(s) 57
Hpy188I TCNGA 1 cut(s) 323
HpyAV CCTTC 1 cut(s) 40
HpyCH4V TGCA 2 cut(s) 25, 405
HpyF10VI GCNNNNNNNGC 1 cut(s) 324
HpyF3I CTNAG 5 cut(s) 42, 99, 292, 305, 328
Hsp92II CATG 2 cut(s) 12, 205
HspAI GCGC 1 cut(s) 400
Kzo9I GATC 1 cut(s) 295
LmnI GCTCC 1 cut(s) 131
LpnPI CCDG 5 cut(s) 81, 111, 165, 312, 360
Lsp1109I GCAGC 2 cut(s) 251, 389
MaeIII GTNAC 2 cut(s) 52, 63
MalI GATC 1 cut(s) 297
MboI GATC 1 cut(s) 295
MflI RGATCY 1 cut(s) 295
MluCI AATT 5 cut(s) 104, 108, 126, 189, 340
MnlI CCTC 4 cut(s) 94, 187, 190, 317
MroXI GAANNNNTTC 1 cut(s) 108
MseI TTAA 1 cut(s) 107
MwoI GCNNNNNNNGC 1 cut(s) 324
NdeII GATC 1 cut(s) 295
NlaIII CATG 2 cut(s) 12, 205
NmuCI GTSAC 1 cut(s) 63
PcsI WCGNNNNNNNCGW 1 cut(s) 214
PdmI GAANNNNTTC 1 cut(s) 108
PfeI GAWTC 2 cut(s) 5, 389
PflMI CCANNNNNTGG 1 cut(s) 74
PkrI GCNGC 2 cut(s) 241, 404
PshBI ATTAAT 1 cut(s) 107
PsiI TTATAA 1 cut(s) 143
PspPI GGNCC 2 cut(s) 148, 182
PsuI RGATCY 1 cut(s) 295
RsaI GTAC 1 cut(s) 303
RsaNI GTAC 1 cut(s) 302
SaqAI TTAA 1 cut(s) 107
SatI GCNGC 2 cut(s) 240, 403
Sau3AI GATC 1 cut(s) 295
Sau96I GGNCC 2 cut(s) 148, 182
SetI ASST 4 cut(s) 34, 100, 244, 289
SinI GGWCC 1 cut(s) 182
Sse9I AATT 5 cut(s) 104, 108, 126, 189, 340
SsiI CCGC 2 cut(s) 363, 371
TaqI TCGA 1 cut(s) 72
TaqII GACCGA 1 cut(s) 268
TasI AATT 5 cut(s) 104, 108, 126, 189, 340
TatI WGTACW 1 cut(s) 301
TfiI GAWTC 2 cut(s) 5, 389
Tru1I TTAA 1 cut(s) 107
Tru9I TTAA 1 cut(s) 107
TscAI CASTG 3 cut(s) 46, 184, 412
TseFI GTSAC 1 cut(s) 63
TseI GCWGC 2 cut(s) 239, 402
Tsp45I GTSAC 1 cut(s) 63
TspDTI ATGAA 1 cut(s) 381
TspRI CASTG 3 cut(s) 46, 184, 412
Van91I CCANNNNNTGG 1 cut(s) 74
VpaK11BI GGWCC 1 cut(s) 182
VspI ATTAAT 1 cut(s) 107
XapI RAATTY 1 cut(s) 340
XcmI CCANNNNNNNNNTGG 1 cut(s) 125
XmnI GAANNNNTTC 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.