Rmu_sc0031707.1_g000001

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0031707.1
Physical Location & Seq
Reverse (-)
1 .. 1712
1712 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0031707.1_g000001.1.cds

Sequence Viewer

Length: 423 bp
atggcccttgatgatgtagtgagaaccaccatcactggtccaagaattgaggaggccatgtatcgtactctacgatggattgacagatgtatagcagctcatacaagaccgagcgagcagaacttatttggtattgttcaaggtggcttagatcctgtactaagggatatatgcgtcagaggcttagtagaacggaatttgcctggctatgccataggcggtcttgcgggtggcgaaagtaaggattcattttggcgtgttgttgctcagtgcactgctgcactgcctgaggacaagccgcgatatgttatgggcgttggttatccattggacattgtagtttgcagtgcgttaggtgctgacatgtatgactgtgtttatccaacacgtactgctcgctttggcacagctcttatacctgag

Protein Analysis

141

Amino Acids

15.46

Weight (kDa)

5.43

Isoelectric Point (pI)

38.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 301
AciI CCGC 3 cut(s) 219, 227, 299
AclWI GGATC 1 cut(s) 146
AcsI RAATTY 1 cut(s) 196
AfaI GTAC 3 cut(s) 67, 159, 391
AflIII ACRYGT 2 cut(s) 363, 386
AgsI TTSAA 1 cut(s) 140
AjnI CCWGG 1 cut(s) 202
AluBI AGCT 2 cut(s) 98, 410
AluI AGCT 2 cut(s) 98, 410
Alw21I GWGCWC 1 cut(s) 275
Alw44I GTGCAC 1 cut(s) 271
AlwI GGATC 1 cut(s) 146
AoxI GGCC 2 cut(s) 3, 54
ApaLI GTGCAC 1 cut(s) 271
ApeKI GCWGC 2 cut(s) 95, 278
ApoI RAATTY 1 cut(s) 196
AspS9I GGNCC 2 cut(s) 4, 38
AvaII GGWCC 1 cut(s) 38
AxyI CCTNAGG 1 cut(s) 288
BaeGI GKGCMC 1 cut(s) 275
Bbv12I GWGCWC 1 cut(s) 275
BbvI GCAGC 2 cut(s) 107, 265
BccI CCATC 2 cut(s) 38, 69
BciT130I CCWGG 1 cut(s) 204
BisI GCNGC 3 cut(s) 96, 279, 299
BlsI GCNGC 3 cut(s) 97, 280, 300
Bme1390I CCNGG 1 cut(s) 204
Bme18I GGWCC 1 cut(s) 38
BmgT120I GGNCC 2 cut(s) 4, 38
BmrFI CCNGG 1 cut(s) 204
BsaAI YACGTR 1 cut(s) 389
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bse1I ACTGG 1 cut(s) 40
Bse21I CCTNAGG 1 cut(s) 288
BseBI CCWGG 1 cut(s) 204
BseMII CTCAG 3 cut(s) 279, 281, 411
BseNI ACTGG 1 cut(s) 40
BseRI GAGGAG 1 cut(s) 65
BseSI GKGCMC 1 cut(s) 275
BseXI GCAGC 2 cut(s) 107, 265
BsgI GTGCAG 1 cut(s) 264
Bsh1236I CGCG 1 cut(s) 301
BshFI GGCC 2 cut(s) 5, 56
BsiHKAI GWGCWC 1 cut(s) 275
BsnI GGCC 2 cut(s) 5, 56
Bsp1286I GDGCHC 1 cut(s) 275
Bsp143I GATC 1 cut(s) 151
BspACI CCGC 3 cut(s) 219, 227, 299
BspANI GGCC 2 cut(s) 5, 56
BspCNI CTCAG 3 cut(s) 280, 280, 412
BspFNI CGCG 1 cut(s) 301
BspPI GGATC 1 cut(s) 146
BsrI ACTGG 1 cut(s) 40
BssMI GATC 1 cut(s) 151
Bst2UI CCWGG 1 cut(s) 204
Bst4CI ACNGT 1 cut(s) 374
BstBAI YACGTR 1 cut(s) 389
BstC8I GCNNGC 2 cut(s) 116, 397
BstDEI CTNAG 6 cut(s) 148, 161, 184, 267, 288, 420
BstFNI CGCG 1 cut(s) 301
BstKTI GATC 1 cut(s) 154
BstMBI GATC 1 cut(s) 151
BstMWI GCNNNNNNNGC 2 cut(s) 180, 356
BstNI CCWGG 1 cut(s) 204
BstNSI RCATGY 1 cut(s) 367
BstSCI CCNGG 1 cut(s) 202
BstSLI GKGCMC 1 cut(s) 275
BstUI CGCG 1 cut(s) 301
BstV1I GCAGC 2 cut(s) 107, 265
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
Bsu36I CCTNAGG 1 cut(s) 288
BsuRI GGCC 2 cut(s) 5, 56
BtsI GCAGTG 3 cut(s) 273, 281, 352
BtsIMutI CAGTG 5 cut(s) 33, 273, 275, 281, 352
Cac8I GCNNGC 2 cut(s) 116, 397
Cfr13I GGNCC 2 cut(s) 4, 38
CseI GACGC 1 cut(s) 163
Csp6I GTAC 3 cut(s) 66, 158, 390
CviAII CATG 2 cut(s) 58, 364
CviJI RGCY 8 cut(s) 5, 56, 98, 147, 183, 207, 298, 410
CviKI_1 RGCY 8 cut(s) 5, 56, 98, 147, 183, 207, 298, 410
CviQI GTAC 3 cut(s) 66, 158, 390
DdeI CTNAG 6 cut(s) 148, 161, 184, 267, 288, 420
DpnI GATC 1 cut(s) 153
DpnII GATC 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 38
Eco81I CCTNAGG 1 cut(s) 288
EcoRII CCWGG 1 cut(s) 202
FaeI CATG 2 cut(s) 61, 367
FatI CATG 2 cut(s) 57, 363
FauI CCCGC 1 cut(s) 220
Fnu4HI GCNGC 3 cut(s) 96, 279, 299
Fsp4HI GCNGC 3 cut(s) 96, 279, 299
GluI GCNGC 3 cut(s) 96, 279, 299
HaeIII GGCC 2 cut(s) 5, 56
HgaI GACGC 1 cut(s) 163
Hin1II CATG 2 cut(s) 61, 367
HinfI GANTC 1 cut(s) 245
Hpy166II GTNNAC 1 cut(s) 273
Hpy188I TCNGA 1 cut(s) 179
Hpy8I GTNNAC 1 cut(s) 273
HpyCH4III ACNGT 1 cut(s) 374
HpyCH4IV ACGT 1 cut(s) 388
HpyCH4V TGCA 3 cut(s) 273, 281, 345
HpyF10VI GCNNNNNNNGC 2 cut(s) 180, 356
HpyF3I CTNAG 6 cut(s) 148, 161, 184, 267, 288, 420
HpySE526I ACGT 1 cut(s) 388
Hsp92II CATG 2 cut(s) 61, 367
Kzo9I GATC 1 cut(s) 151
LpnPI CCDG 5 cut(s) 21, 168, 189, 216, 300
Lsp1109I GCAGC 2 cut(s) 107, 265
MaeII ACGT 1 cut(s) 388
MalI GATC 1 cut(s) 153
MboI GATC 1 cut(s) 151
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 1 cut(s) 275
MluCI AATT 2 cut(s) 45, 196
MmeI TCCRAC 1 cut(s) 407
MnlI CCTC 4 cut(s) 43, 46, 173, 283
MspR9I CCNGG 1 cut(s) 204
MvaI CCWGG 1 cut(s) 204
MvnI CGCG 1 cut(s) 301
MwoI GCNNNNNNNGC 2 cut(s) 180, 356
NdeII GATC 1 cut(s) 151
NlaIII CATG 2 cut(s) 61, 367
NspI RCATGY 1 cut(s) 367
PciI ACATGT 1 cut(s) 363
PcsI WCGNNNNNNNCGW 1 cut(s) 70
PfeI GAWTC 1 cut(s) 245
PkrI GCNGC 3 cut(s) 97, 280, 300
Ppu21I YACGTR 1 cut(s) 389
PscI ACATGT 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 202
PspGI CCWGG 1 cut(s) 202
PspPI GGNCC 2 cut(s) 4, 38
PsuI RGATCY 1 cut(s) 151
RsaI GTAC 3 cut(s) 67, 159, 391
RsaNI GTAC 3 cut(s) 66, 158, 390
SatI GCNGC 3 cut(s) 96, 279, 299
Sau3AI GATC 1 cut(s) 151
Sau96I GGNCC 2 cut(s) 4, 38
ScrFI CCNGG 1 cut(s) 204
SduI GDGCHC 1 cut(s) 275
SetI ASST 6 cut(s) 100, 145, 358, 391, 412, 421
SinI GGWCC 1 cut(s) 38
Sse9I AATT 2 cut(s) 45, 196
SsiI CCGC 3 cut(s) 219, 227, 299
StyD4I CCNGG 1 cut(s) 202
TaaI ACNGT 1 cut(s) 374
TaiI ACGT 1 cut(s) 391
TaqII GACCGA 1 cut(s) 124
TasI AATT 2 cut(s) 45, 196
TatI WGTACW 1 cut(s) 157
TauI GCSGC 1 cut(s) 301
TfiI GAWTC 1 cut(s) 245
TscAI CASTG 5 cut(s) 40, 275, 280, 288, 352
TseI GCWGC 2 cut(s) 95, 278
TspDTI ATGAA 1 cut(s) 237
TspGWI ACGGA 1 cut(s) 208
TspRI CASTG 5 cut(s) 40, 275, 280, 288, 352
VneI GTGCAC 1 cut(s) 271
VpaK11BI GGWCC 1 cut(s) 38
XapI RAATTY 1 cut(s) 196
XceI RCATGY 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.