Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_5g36560 FvH4_5g36560 FvH4_5g36560 FvH4_5g36560 FvH4_5g36560 FvH4_5g36560 |
| malus_domestica | MD08G1188600.v1.1 MD15G1376400.v1.1 |
| prunus_persica | Prupe.1G523100_v2.0.a1 Prupe.1G523100_v2.0.a1 |
| pyrus_communis | pycom08g16230 pycom15g33630 |
| rosa_chinensis | RchiOBHm_Chr3g0492071 RchiOBHm_Chr7g0242241 |
| rosa_laevigata | RLG00000000904 RLG00000022795 |
| rosa_multiflora | Rmu_sc0003846.1_g000008 Rmu_sc0031707.1_g000001 |
| rosa_roxburghii | Rroxscaffold_6G00392560 |
| rosa_rugosa | Rorug03G0251600 Rorug03G0251700 Rorug07G0314300 |
| rosa_samantha | Rh3AG301800 Rh3BG337600 Rh3BG337700 Rh3CG335200 Rh3DG337300 Rh7AG469700 Rh7BG439500 Rh7CG486300 Rh7DG454700 |
| rosa_wichuraiana | Rw3G026640 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 301 |
| AciI | CCGC | 3 cut(s) | 219, 227, 299 |
| AclWI | GGATC | 1 cut(s) | 146 |
| AcsI | RAATTY | 1 cut(s) | 196 |
| AfaI | GTAC | 3 cut(s) | 67, 159, 391 |
| AflIII | ACRYGT | 2 cut(s) | 363, 386 |
| AgsI | TTSAA | 1 cut(s) | 140 |
| AjnI | CCWGG | 1 cut(s) | 202 |
| AluBI | AGCT | 2 cut(s) | 98, 410 |
| AluI | AGCT | 2 cut(s) | 98, 410 |
| Alw21I | GWGCWC | 1 cut(s) | 275 |
| Alw44I | GTGCAC | 1 cut(s) | 271 |
| AlwI | GGATC | 1 cut(s) | 146 |
| AoxI | GGCC | 2 cut(s) | 3, 54 |
| ApaLI | GTGCAC | 1 cut(s) | 271 |
| ApeKI | GCWGC | 2 cut(s) | 95, 278 |
| ApoI | RAATTY | 1 cut(s) | 196 |
| AspS9I | GGNCC | 2 cut(s) | 4, 38 |
| AvaII | GGWCC | 1 cut(s) | 38 |
| AxyI | CCTNAGG | 1 cut(s) | 288 |
| BaeGI | GKGCMC | 1 cut(s) | 275 |
| Bbv12I | GWGCWC | 1 cut(s) | 275 |
| BbvI | GCAGC | 2 cut(s) | 107, 265 |
| BccI | CCATC | 2 cut(s) | 38, 69 |
| BciT130I | CCWGG | 1 cut(s) | 204 |
| BisI | GCNGC | 3 cut(s) | 96, 279, 299 |
| BlsI | GCNGC | 3 cut(s) | 97, 280, 300 |
| Bme1390I | CCNGG | 1 cut(s) | 204 |
| Bme18I | GGWCC | 1 cut(s) | 38 |
| BmgT120I | GGNCC | 2 cut(s) | 4, 38 |
| BmrFI | CCNGG | 1 cut(s) | 204 |
| BsaAI | YACGTR | 1 cut(s) | 389 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 44, 74 |
| Bse1I | ACTGG | 1 cut(s) | 40 |
| Bse21I | CCTNAGG | 1 cut(s) | 288 |
| BseBI | CCWGG | 1 cut(s) | 204 |
| BseMII | CTCAG | 3 cut(s) | 279, 281, 411 |
| BseNI | ACTGG | 1 cut(s) | 40 |
| BseRI | GAGGAG | 1 cut(s) | 65 |
| BseSI | GKGCMC | 1 cut(s) | 275 |
| BseXI | GCAGC | 2 cut(s) | 107, 265 |
| BsgI | GTGCAG | 1 cut(s) | 264 |
| Bsh1236I | CGCG | 1 cut(s) | 301 |
| BshFI | GGCC | 2 cut(s) | 5, 56 |
| BsiHKAI | GWGCWC | 1 cut(s) | 275 |
| BsnI | GGCC | 2 cut(s) | 5, 56 |
| Bsp1286I | GDGCHC | 1 cut(s) | 275 |
| Bsp143I | GATC | 1 cut(s) | 151 |
| BspACI | CCGC | 3 cut(s) | 219, 227, 299 |
| BspANI | GGCC | 2 cut(s) | 5, 56 |
| BspCNI | CTCAG | 3 cut(s) | 280, 280, 412 |
| BspFNI | CGCG | 1 cut(s) | 301 |
| BspPI | GGATC | 1 cut(s) | 146 |
| BsrI | ACTGG | 1 cut(s) | 40 |
| BssMI | GATC | 1 cut(s) | 151 |
| Bst2UI | CCWGG | 1 cut(s) | 204 |
| Bst4CI | ACNGT | 1 cut(s) | 374 |
| BstBAI | YACGTR | 1 cut(s) | 389 |
| BstC8I | GCNNGC | 2 cut(s) | 116, 397 |
| BstDEI | CTNAG | 6 cut(s) | 148, 161, 184, 267, 288, 420 |
| BstFNI | CGCG | 1 cut(s) | 301 |
| BstKTI | GATC | 1 cut(s) | 154 |
| BstMBI | GATC | 1 cut(s) | 151 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 180, 356 |
| BstNI | CCWGG | 1 cut(s) | 204 |
| BstNSI | RCATGY | 1 cut(s) | 367 |
| BstSCI | CCNGG | 1 cut(s) | 202 |
| BstSLI | GKGCMC | 1 cut(s) | 275 |
| BstUI | CGCG | 1 cut(s) | 301 |
| BstV1I | GCAGC | 2 cut(s) | 107, 265 |
| BstX2I | RGATCY | 1 cut(s) | 151 |
| BstYI | RGATCY | 1 cut(s) | 151 |
| Bsu36I | CCTNAGG | 1 cut(s) | 288 |
| BsuRI | GGCC | 2 cut(s) | 5, 56 |
| BtsI | GCAGTG | 3 cut(s) | 273, 281, 352 |
| BtsIMutI | CAGTG | 5 cut(s) | 33, 273, 275, 281, 352 |
| Cac8I | GCNNGC | 2 cut(s) | 116, 397 |
| Cfr13I | GGNCC | 2 cut(s) | 4, 38 |
| CseI | GACGC | 1 cut(s) | 163 |
| Csp6I | GTAC | 3 cut(s) | 66, 158, 390 |
| CviAII | CATG | 2 cut(s) | 58, 364 |
| CviJI | RGCY | 8 cut(s) | 5, 56, 98, 147, 183, 207, 298, 410 |
| CviKI_1 | RGCY | 8 cut(s) | 5, 56, 98, 147, 183, 207, 298, 410 |
| CviQI | GTAC | 3 cut(s) | 66, 158, 390 |
| DdeI | CTNAG | 6 cut(s) | 148, 161, 184, 267, 288, 420 |
| DpnI | GATC | 1 cut(s) | 153 |
| DpnII | GATC | 1 cut(s) | 151 |
| Eco47I | GGWCC | 1 cut(s) | 38 |
| Eco81I | CCTNAGG | 1 cut(s) | 288 |
| EcoRII | CCWGG | 1 cut(s) | 202 |
| FaeI | CATG | 2 cut(s) | 61, 367 |
| FatI | CATG | 2 cut(s) | 57, 363 |
| FauI | CCCGC | 1 cut(s) | 220 |
| Fnu4HI | GCNGC | 3 cut(s) | 96, 279, 299 |
| Fsp4HI | GCNGC | 3 cut(s) | 96, 279, 299 |
| GluI | GCNGC | 3 cut(s) | 96, 279, 299 |
| HaeIII | GGCC | 2 cut(s) | 5, 56 |
| HgaI | GACGC | 1 cut(s) | 163 |
| Hin1II | CATG | 2 cut(s) | 61, 367 |
| HinfI | GANTC | 1 cut(s) | 245 |
| Hpy166II | GTNNAC | 1 cut(s) | 273 |
| Hpy188I | TCNGA | 1 cut(s) | 179 |
| Hpy8I | GTNNAC | 1 cut(s) | 273 |
| HpyCH4III | ACNGT | 1 cut(s) | 374 |
| HpyCH4IV | ACGT | 1 cut(s) | 388 |
| HpyCH4V | TGCA | 3 cut(s) | 273, 281, 345 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 180, 356 |
| HpyF3I | CTNAG | 6 cut(s) | 148, 161, 184, 267, 288, 420 |
| HpySE526I | ACGT | 1 cut(s) | 388 |
| Hsp92II | CATG | 2 cut(s) | 61, 367 |
| Kzo9I | GATC | 1 cut(s) | 151 |
| LpnPI | CCDG | 5 cut(s) | 21, 168, 189, 216, 300 |
| Lsp1109I | GCAGC | 2 cut(s) | 107, 265 |
| MaeII | ACGT | 1 cut(s) | 388 |
| MalI | GATC | 1 cut(s) | 153 |
| MboI | GATC | 1 cut(s) | 151 |
| MflI | RGATCY | 1 cut(s) | 151 |
| MhlI | GDGCHC | 1 cut(s) | 275 |
| MluCI | AATT | 2 cut(s) | 45, 196 |
| MmeI | TCCRAC | 1 cut(s) | 407 |
| MnlI | CCTC | 4 cut(s) | 43, 46, 173, 283 |
| MspR9I | CCNGG | 1 cut(s) | 204 |
| MvaI | CCWGG | 1 cut(s) | 204 |
| MvnI | CGCG | 1 cut(s) | 301 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 180, 356 |
| NdeII | GATC | 1 cut(s) | 151 |
| NlaIII | CATG | 2 cut(s) | 61, 367 |
| NspI | RCATGY | 1 cut(s) | 367 |
| PciI | ACATGT | 1 cut(s) | 363 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 70 |
| PfeI | GAWTC | 1 cut(s) | 245 |
| PkrI | GCNGC | 3 cut(s) | 97, 280, 300 |
| Ppu21I | YACGTR | 1 cut(s) | 389 |
| PscI | ACATGT | 1 cut(s) | 363 |
| Psp6I | CCWGG | 1 cut(s) | 202 |
| PspGI | CCWGG | 1 cut(s) | 202 |
| PspPI | GGNCC | 2 cut(s) | 4, 38 |
| PsuI | RGATCY | 1 cut(s) | 151 |
| RsaI | GTAC | 3 cut(s) | 67, 159, 391 |
| RsaNI | GTAC | 3 cut(s) | 66, 158, 390 |
| SatI | GCNGC | 3 cut(s) | 96, 279, 299 |
| Sau3AI | GATC | 1 cut(s) | 151 |
| Sau96I | GGNCC | 2 cut(s) | 4, 38 |
| ScrFI | CCNGG | 1 cut(s) | 204 |
| SduI | GDGCHC | 1 cut(s) | 275 |
| SetI | ASST | 6 cut(s) | 100, 145, 358, 391, 412, 421 |
| SinI | GGWCC | 1 cut(s) | 38 |
| Sse9I | AATT | 2 cut(s) | 45, 196 |
| SsiI | CCGC | 3 cut(s) | 219, 227, 299 |
| StyD4I | CCNGG | 1 cut(s) | 202 |
| TaaI | ACNGT | 1 cut(s) | 374 |
| TaiI | ACGT | 1 cut(s) | 391 |
| TaqII | GACCGA | 1 cut(s) | 124 |
| TasI | AATT | 2 cut(s) | 45, 196 |
| TatI | WGTACW | 1 cut(s) | 157 |
| TauI | GCSGC | 1 cut(s) | 301 |
| TfiI | GAWTC | 1 cut(s) | 245 |
| TscAI | CASTG | 5 cut(s) | 40, 275, 280, 288, 352 |
| TseI | GCWGC | 2 cut(s) | 95, 278 |
| TspDTI | ATGAA | 1 cut(s) | 237 |
| TspGWI | ACGGA | 1 cut(s) | 208 |
| TspRI | CASTG | 5 cut(s) | 40, 275, 280, 288, 352 |
| VneI | GTGCAC | 1 cut(s) | 271 |
| VpaK11BI | GGWCC | 1 cut(s) | 38 |
| XapI | RAATTY | 1 cut(s) | 196 |
| XceI | RCATGY | 1 cut(s) | 367 |