Rh7DG454700

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0004542
Physical Location & Seq
Forward (+)
64710395 .. 64717085
6691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG454700.1

Sequence Viewer

Length: 1203 bp
ATGGCGCTTCGTTTCGAGGTTTTGGGGAGGTTCAATCGCGCTCGTGCAGCTCAACTAACACTCCCTCACTTTGTATGTCAGACTCCTCTTTTCATGCCTGTTGGAACACAAGGAACTATAAAAGGGTTGGCAACTGACCAGCTTGAGGAGATTGGTTGCCAAATAATTCTTGGGAATACATATCATTTGGCACTTCGACCTACCTCTGAGCTTATTGACGAGATGGGAGGCCTCCACAAATTTATGAACTGGCCAAGAGCGCTGCTTACTGATTCTGGTGGCTTTCAGATGGTATCCTTATTGCATTTAGCTGACATCACTGAGAAGGGTGTAACATTTCAGTCACCAGTCGATGGAAAGCCAATGCTCTTGACACCTGAGGAATCAATTCAAATCCAAAATAGAATTGGAGCAGATATTATAATGGCCCTTGATGATGTAGTGAAAACCACCATCACTGGTCCAAGAATTGAGGAGGCCATGTATCGTACTCTACGATGGATTGACAGATGTATAGCAGCTCATACAAGACCGAGCGAGCAGAACTTATTTGGTATTGTTCAAGGTGGCTTAGATCCTGTACTAAGGGATATATGCGTCAGAGGCTTAGTAGAACGGAATTTGCCTGGCTATGCCATAGGCGGTCTTGCGGGTGGCGAAGGTAAAGATTCATTTTGGCGCGTTGTTGCTCAGTGCACTGCTGCACTGCCTGAGGACAAGCCGCGATATGTTATGGGTGTTGGTTATCCATTGGACATTGTAGTTTGCAGTGCTTTAGGTGCTGACATGTATGACTGTGTTTATCCAACACGTACTGCTCGCTTTGGCACAGCTCTTATACCTGAGGGAGTGCTGAAGCTGAAGCACAAAGCTATGGCTGAGGATATCCGCCCTATCGATCCTACATGTGCTTGTATGGTATGCAAGAATTATACTAGAGCTTACATTCATTGCCTAGTTACAAAAGATGCTATGGGATCTCAGCTTCTGTCATATCACAATCTGTATTACATGCTAAAGCTAAGTAGAGATCTACACTCGTCAATAATCCAAGGAACATTTCCAGAGTTTGTATGCAACTTTCTGCAAACAATGTTTCCTAAAGGCGATGTTCCAGAATGGGTCTGTAATGCCATGGAGGTTGCTGGAATTGACATCACTTCCTGTTGTACTGCGTTTTCTTCATCTCAAGATTCAGATTAG

Protein Analysis

400

Amino Acids

44.17

Weight (kDa)

5.75

Isoelectric Point (pI)

40.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 13 - 365 3.2e-152 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 422
AccB7I CCANNNNNTGG 1 cut(s) 353
AccII CGCG 3 cut(s) 39, 681, 724
AciI CCGC 4 cut(s) 642, 650, 722, 889
AclWI GGATC 3 cut(s) 569, 893, 985
AcoI YGGCCR 1 cut(s) 251
AcsI RAATTY 2 cut(s) 239, 619
AcuI CTGAAG 2 cut(s) 875, 881
AfaI GTAC 4 cut(s) 490, 582, 814, 1171
AfeI AGCGCT 1 cut(s) 261
AfiI CCNNNNNNNGG 2 cut(s) 145, 353
AflIII ACRYGT 3 cut(s) 786, 809, 905
AgsI TTSAA 3 cut(s) 34, 392, 563
AjnI CCWGG 1 cut(s) 625
AjuI GAANNNNNNNTTGG 2 cut(s) 1044, 1076
Alw21I GWGCWC 1 cut(s) 698
Alw44I GTGCAC 1 cut(s) 694
AlwI GGATC 3 cut(s) 569, 893, 985
AlwNI CAGNNNCTG 1 cut(s) 988
Aor51HI AGCGCT 1 cut(s) 261
AoxI GGCC 4 cut(s) 229, 251, 426, 477
ApaLI GTGCAC 1 cut(s) 694
ApeKI GCWGC 4 cut(s) 47, 262, 518, 701
ApoI RAATTY 2 cut(s) 239, 619
Asp700I GAANNNNTTC 1 cut(s) 387
AspLEI GCGC 4 cut(s) 7, 41, 262, 681
AspS9I GGNCC 2 cut(s) 427, 461
AsuHPI GGTGA 1 cut(s) 336
AvaII GGWCC 1 cut(s) 461
AxyI CCTNAGG 3 cut(s) 378, 711, 843
BaeGI GKGCMC 1 cut(s) 698
BalI TGGCCA 1 cut(s) 253
BauI CACGAG 1 cut(s) 42
Bbv12I GWGCWC 1 cut(s) 698
BbvCI CCTCAGC 1 cut(s) 879
BbvI GCAGC 4 cut(s) 59, 249, 530, 688
BccI CCATC 5 cut(s) 217, 283, 347, 461, 492
BciT130I CCWGG 1 cut(s) 627
BciVI GTATCC 1 cut(s) 304
BfaI CTAG 2 cut(s) 936, 956
BfoI RGCGCY 2 cut(s) 8, 263
BfuI GTATCC 1 cut(s) 304
BglII AGATCT 1 cut(s) 1030
BisI GCNGC 5 cut(s) 48, 263, 519, 702, 722
BlsI GCNGC 5 cut(s) 49, 264, 520, 703, 723
Bme1390I CCNGG 1 cut(s) 627
Bme18I GGWCC 1 cut(s) 461
BmgT120I GGNCC 2 cut(s) 427, 461
BmrFI CCNGG 1 cut(s) 627
BmsI GCATC 1 cut(s) 958
Bpu10I CCTNAGC 1 cut(s) 879
BpuEI CTTGAG 2 cut(s) 164, 1173
Bsa29I ATCGAT 1 cut(s) 897
BsaAI YACGTR 1 cut(s) 812
BsaJI CCNNGG 2 cut(s) 1051, 1134
BsaXI ACNNNNNCTCC 4 cut(s) 45, 75, 467, 497
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 353
Bse1I ACTGG 3 cut(s) 254, 347, 463
Bse21I CCTNAGG 3 cut(s) 378, 711, 843
Bse3DI GCAATG 1 cut(s) 949
BseBI CCWGG 1 cut(s) 627
BseCI ATCGAT 1 cut(s) 897
BseDI CCNNGG 2 cut(s) 1051, 1134
BseLI CCNNNNNNNGG 2 cut(s) 145, 353
BseMI GCAATG 1 cut(s) 949
BseMII CTCAG 8 cut(s) 198, 312, 369, 702, 704, 834, 870, 995
BseNI ACTGG 3 cut(s) 254, 347, 463
BseRI GAGGAG 3 cut(s) 75, 161, 488
BseSI GKGCMC 1 cut(s) 698
BseXI GCAGC 4 cut(s) 59, 249, 530, 688
BsgI GTGCAG 2 cut(s) 66, 687
Bsh1236I CGCG 3 cut(s) 39, 681, 724
BshFI GGCC 4 cut(s) 231, 253, 428, 479
BshVI ATCGAT 1 cut(s) 897
BsiHKAI GWGCWC 1 cut(s) 698
BslI CCNNNNNNNGG 2 cut(s) 145, 353
BsnI GGCC 4 cut(s) 231, 253, 428, 479
Bsp1286I GDGCHC 1 cut(s) 698
Bsp143I GATC 4 cut(s) 574, 898, 977, 1030
Bsp19I CCATGG 1 cut(s) 1134
BspACI CCGC 4 cut(s) 642, 650, 722, 889
BspANI GGCC 4 cut(s) 231, 253, 428, 479
BspCNI CTCAG 8 cut(s) 199, 313, 370, 703, 703, 835, 871, 994
BspDI ATCGAT 1 cut(s) 897
BspFNI CGCG 3 cut(s) 39, 681, 724
BspPI GGATC 3 cut(s) 569, 893, 985
BsrDI GCAATG 1 cut(s) 949
BsrI ACTGG 3 cut(s) 254, 347, 463
BssECI CCNNGG 2 cut(s) 1051, 1134
BssMI GATC 4 cut(s) 574, 898, 977, 1030
BssSI CACGAG 1 cut(s) 42
BssT1I CCWWGG 2 cut(s) 1051, 1134
Bst2BI CACGAG 1 cut(s) 42
Bst2UI CCWGG 1 cut(s) 627
Bst4CI ACNGT 1 cut(s) 797
BstBAI YACGTR 1 cut(s) 812
BstC8I GCNNGC 2 cut(s) 539, 820
BstDSI CCRYGG 1 cut(s) 1134
BstFNI CGCG 3 cut(s) 39, 681, 724
BstH2I RGCGCY 2 cut(s) 8, 263
BstHHI GCGC 4 cut(s) 7, 41, 262, 681
BstKTI GATC 4 cut(s) 577, 901, 980, 1033
BstMBI GATC 4 cut(s) 574, 898, 977, 1030
BstMWI GCNNNNNNNGC 4 cut(s) 47, 259, 603, 779
BstNI CCWGG 1 cut(s) 627
BstNSI RCATGY 3 cut(s) 790, 909, 1015
BstSCI CCNGG 1 cut(s) 625
BstSLI GKGCMC 1 cut(s) 698
BstUI CGCG 3 cut(s) 39, 681, 724
BstV1I GCAGC 4 cut(s) 59, 249, 530, 688
BstX2I RGATCY 3 cut(s) 574, 977, 1030
BstYI RGATCY 3 cut(s) 574, 977, 1030
Bsu15I ATCGAT 1 cut(s) 897
Bsu36I CCTNAGG 3 cut(s) 378, 711, 843
BsuI GTATCC 1 cut(s) 304
BsuRI GGCC 4 cut(s) 231, 253, 428, 479
BsuTUI ATCGAT 1 cut(s) 897
BtgI CCRYGG 1 cut(s) 1134
BtgZI GCGATG 1 cut(s) 1122
BtsI GCAGTG 3 cut(s) 696, 704, 775
BtsIMutI CAGTG 6 cut(s) 318, 456, 696, 698, 704, 775
Cac8I GCNNGC 2 cut(s) 539, 820
CaiI CAGNNNCTG 1 cut(s) 988
CfoI GCGC 4 cut(s) 7, 41, 262, 681
Cfr13I GGNCC 2 cut(s) 427, 461
ClaI ATCGAT 1 cut(s) 897
CseI GACGC 1 cut(s) 586
Csp6I GTAC 4 cut(s) 489, 581, 813, 1170
CviAII CATG 6 cut(s) 94, 481, 787, 906, 1012, 1135
CviQI GTAC 4 cut(s) 489, 581, 813, 1170
DpnI GATC 4 cut(s) 576, 900, 979, 1032
DpnII GATC 4 cut(s) 574, 898, 977, 1030
EaeI YGGCCR 1 cut(s) 251
EciI GGCGGA 1 cut(s) 878
Eco130I CCWWGG 2 cut(s) 1051, 1134
Eco147I AGGCCT 1 cut(s) 231
Eco32I GATATC 1 cut(s) 886
Eco47I GGWCC 1 cut(s) 461
Eco47III AGCGCT 1 cut(s) 261
Eco57I CTGAAG 2 cut(s) 875, 881
Eco81I CCTNAGG 3 cut(s) 378, 711, 843
EcoRII CCWGG 1 cut(s) 625
EcoRV GATATC 1 cut(s) 886
EcoT14I CCWWGG 2 cut(s) 1051, 1134
ErhI CCWWGG 2 cut(s) 1051, 1134
FaeI CATG 6 cut(s) 97, 484, 790, 909, 1015, 1138
FatI CATG 6 cut(s) 93, 480, 786, 905, 1011, 1134
FauI CCCGC 1 cut(s) 643
Fnu4HI GCNGC 5 cut(s) 48, 263, 519, 702, 722
Fsp4HI GCNGC 5 cut(s) 48, 263, 519, 702, 722
FspBI CTAG 2 cut(s) 936, 956
GlaI GCGC 4 cut(s) 6, 40, 261, 680
GluI GCNGC 5 cut(s) 48, 263, 519, 702, 722
HaeII RGCGCY 2 cut(s) 8, 263
HaeIII GGCC 4 cut(s) 231, 253, 428, 479
HgaI GACGC 1 cut(s) 586
HhaI GCGC 4 cut(s) 7, 41, 262, 681
Hin1II CATG 6 cut(s) 97, 484, 790, 909, 1015, 1138
Hin6I GCGC 4 cut(s) 5, 39, 260, 679
HinP1I GCGC 4 cut(s) 5, 39, 260, 679
HinfI GANTC 5 cut(s) 82, 272, 383, 668, 1193
HphI GGTGA 1 cut(s) 336
Hpy166II GTNNAC 1 cut(s) 696
Hpy188I TCNGA 5 cut(s) 81, 208, 288, 602, 1198
Hpy188III TCNNGA 4 cut(s) 370, 1064, 1115, 1190
Hpy8I GTNNAC 1 cut(s) 696
HpyAV CCTTC 2 cut(s) 319, 653
HpyCH4III ACNGT 1 cut(s) 797
HpyCH4IV ACGT 1 cut(s) 811
HpyCH4V TGCA 8 cut(s) 47, 304, 696, 704, 768, 924, 1077, 1087
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 259, 603, 779
HpySE526I ACGT 1 cut(s) 811
Hsp92II CATG 6 cut(s) 97, 484, 790, 909, 1015, 1138
HspAI GCGC 4 cut(s) 5, 39, 260, 679
Kzo9I GATC 4 cut(s) 574, 898, 977, 1030
LmnI GCTCC 1 cut(s) 410
Lsp1109I GCAGC 4 cut(s) 59, 249, 530, 688
LweI GCATC 1 cut(s) 958
MaeI CTAG 2 cut(s) 936, 956
MaeII ACGT 1 cut(s) 811
MaeIII GTNAC 3 cut(s) 331, 342, 958
MalI GATC 4 cut(s) 576, 900, 979, 1032
MboI GATC 4 cut(s) 574, 898, 977, 1030
MboII GAAGA 1 cut(s) 1173
MflI RGATCY 3 cut(s) 574, 977, 1030
MhlI GDGCHC 1 cut(s) 698
MlsI TGGCCA 1 cut(s) 253
MluCI AATT 8 cut(s) 165, 239, 387, 405, 468, 619, 928, 1149
MluNI TGGCCA 1 cut(s) 253
MlyI GAGTC 1 cut(s) 76
MmeI TCCRAC 2 cut(s) 82, 830
Mox20I TGGCCA 1 cut(s) 253
MroXI GAANNNNTTC 1 cut(s) 387
MscI TGGCCA 1 cut(s) 253
Msp20I TGGCCA 1 cut(s) 253
MspR9I CCNGG 1 cut(s) 627
MvaI CCWGG 1 cut(s) 627
MvnI CGCG 3 cut(s) 39, 681, 724
MwoI GCNNNNNNNGC 4 cut(s) 47, 259, 603, 779
NcoI CCATGG 1 cut(s) 1134
NdeII GATC 4 cut(s) 574, 898, 977, 1030
NlaIII CATG 6 cut(s) 97, 484, 790, 909, 1015, 1138
NmuCI GTSAC 1 cut(s) 342
NspI RCATGY 3 cut(s) 790, 909, 1015
PceI AGGCCT 1 cut(s) 231
PciI ACATGT 2 cut(s) 786, 905
PcsI WCGNNNNNNNCGW 1 cut(s) 493
PdmI GAANNNNTTC 1 cut(s) 387
PfeI GAWTC 4 cut(s) 272, 383, 668, 1193
PflMI CCANNNNNTGG 1 cut(s) 353
PkrI GCNGC 5 cut(s) 49, 264, 520, 703, 723
PleI GAGTC 1 cut(s) 76
PpsI GAGTC 1 cut(s) 76
Ppu21I YACGTR 1 cut(s) 812
PscI ACATGT 2 cut(s) 786, 905
PsiI TTATAA 1 cut(s) 422
Psp6I CCWGG 1 cut(s) 625
PspGI CCWGG 1 cut(s) 625
PspPI GGNCC 2 cut(s) 427, 461
PstNI CAGNNNCTG 1 cut(s) 988
PsuI RGATCY 3 cut(s) 574, 977, 1030
RsaI GTAC 4 cut(s) 490, 582, 814, 1171
RsaNI GTAC 4 cut(s) 489, 581, 813, 1170
SatI GCNGC 5 cut(s) 48, 263, 519, 702, 722
Sau3AI GATC 4 cut(s) 574, 898, 977, 1030
Sau96I GGNCC 2 cut(s) 427, 461
SchI GAGTC 1 cut(s) 76
ScrFI CCNGG 1 cut(s) 627
SduI GDGCHC 1 cut(s) 698
SfaNI GCATC 1 cut(s) 958
SinI GGWCC 1 cut(s) 461
SmlI CTYRAG 2 cut(s) 143, 1188
SmoI CTYRAG 2 cut(s) 143, 1188
Sse9I AATT 8 cut(s) 165, 239, 387, 405, 468, 619, 928, 1149
SseBI AGGCCT 1 cut(s) 231
SsiI CCGC 4 cut(s) 642, 650, 722, 889
SspMI CTAG 2 cut(s) 936, 956
StuI AGGCCT 1 cut(s) 231
StyD4I CCNGG 1 cut(s) 625
StyI CCWWGG 2 cut(s) 1051, 1134
TaaI ACNGT 1 cut(s) 797
TaiI ACGT 1 cut(s) 814
TaqI TCGA 4 cut(s) 15, 196, 351, 897
TaqII GACCGA 1 cut(s) 547
TasI AATT 8 cut(s) 165, 239, 387, 405, 468, 619, 928, 1149
TatI WGTACW 2 cut(s) 580, 1169
TauI GCSGC 1 cut(s) 724
TfiI GAWTC 4 cut(s) 272, 383, 668, 1193
TscAI CASTG 6 cut(s) 325, 463, 698, 703, 711, 775
TseFI GTSAC 1 cut(s) 342
TseI GCWGC 4 cut(s) 47, 262, 518, 701
Tsp45I GTSAC 1 cut(s) 342
TspDTI ATGAA 5 cut(s) 82, 260, 660, 938, 1173
TspGWI ACGGA 1 cut(s) 631
TspRI CASTG 6 cut(s) 325, 463, 698, 703, 711, 775
Van91I CCANNNNNTGG 1 cut(s) 353
VneI GTGCAC 1 cut(s) 694
VpaK11BI GGWCC 1 cut(s) 461
XapI RAATTY 2 cut(s) 239, 619
XceI RCATGY 3 cut(s) 790, 909, 1015
XcmI CCANNNNNNNNNTGG 2 cut(s) 167, 404
XmnI GAANNNNTTC 1 cut(s) 387
XspI CTAG 2 cut(s) 936, 956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.