MD08G1188600.v1.1

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
Physical Location & Seq
Reverse (-)
23807912 .. 23822800
14889 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1188600.v1.1.491

Sequence Viewer

Length: 1197 bp
ATGGCGCTTCGTTTCGAGGTTTTGGGGAGGTTTAACCGTGCTCGTGCAGCTAGATTGACCCTGCCTCACTTTGTGTGCCAGACACCACTTTTCATGCCCGTTGGAACGCAAGGAACTATTAAAGGGTTGACAACTGAACAGCTTGAAGATATTGGTGCTCAAATAATACTTGGCAATACTTATCATTTGGCACTTCGTCCGACTTCCGAGCTTCTTGATGAGTTGGGAGGTCTCCACAAATTTATGAACTGGCCAAGAGCATTGCTTACTGATTCCGGTGGCTTTCAGATGGTATCATTATTGCATTTAGCTGACATCACCGAAGAAGGTGTAACATTTCAGTCACCAGTTGATGGAAAGCCAATGCTTTTGACACCTGAGGAGTCAATTCAAATCCAAAACAGAATTGGAGCAGATATTATTATGGCCCTGGATGATGTAGTCAAAACCACCACCACTGGTCCAAGAGTTGAGGAGGCCATGTATCGCACTATACGATGGATAGACAGATGCATTGAAGCTCACAAGAGACCAACGGAGCAGAACTTATTTGGTATTGTACAAGGTGGCTTAGATCCTGTATTAAGAGATATATGTGTAAAGGGCTTGGTGAATCGAAACTTACCCGGGTATGCTATTGGCGGTCTTTCTGGTGGTGAAAGTAAGGATTCATTTTGGCGTGTTGTTGCACAGTGCACTGCTGCATTGCCTGAGGAGAAACCGCGATATGTTATGGGTGTTGGTTATCCTCTGGATATTGTAGTTTGCAGTGCTCTAGGTGCTGACATGTATGACTGTGTTTATCCCACACGTACGGCTCGCTTTGGTACAGCTCTTGTACCCGAGGGAGTGCTGAAACTCAAACAGAAAGCGATGGCTGAAGATACCCGCCCCGTTGATCCTACATGTGATTGTATGGTCTGCAAAAATTATACTAGAGCGTACATTCATTGCCTTGTTACAAAAGATGCTATGGGATCTCAGCTTCTGTCATATCACAATTTATATTACATGATGAAGCTAAGTAGAAATCTGCACTCTTCAATAATCAAGGGAAGGTTTCCAGAGTTTGTACGTGAATTTCTGCAACAAATGTTTCCGAAAGGTGATATCCCCGAGTGGGTCTGTGATGCCATGGAGGTTGCTGGAATCGACATTTCTTCCTGCTGCACTCCGTTTTCATCATCCCAAGATTAG

Protein Analysis

399

Amino Acids

44.25

Weight (kDa)

5.96

Isoelectric Point (pI)

44.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 13 - 365 1.9e-155 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 353
AccII CGCG 1 cut(s) 724
AciI CCGC 3 cut(s) 642, 722, 889
AclWI GGATC 3 cut(s) 569, 893, 985
AcoI YGGCCR 1 cut(s) 251
AcsI RAATTY 2 cut(s) 239, 1079
AcuI CTGAAG 1 cut(s) 900
AdeI CACNNNGTG 1 cut(s) 73
AfaI GTAC 6 cut(s) 561, 814, 829, 840, 944, 1074
AfiI CCNNNNNNNGG 2 cut(s) 353, 1120
AflIII ACRYGT 3 cut(s) 786, 809, 905
AgsI TTSAA 4 cut(s) 146, 392, 518, 1044
AjnI CCWGG 1 cut(s) 429
AluBI AGCT 8 cut(s) 50, 142, 211, 311, 521, 833, 985, 1021
AluI AGCT 8 cut(s) 50, 142, 211, 311, 521, 833, 985, 1021
Alw21I GWGCWC 4 cut(s) 43, 160, 698, 775
Alw26I GTCTC 2 cut(s) 236, 523
Alw44I GTGCAC 1 cut(s) 694
AlwI GGATC 3 cut(s) 569, 893, 985
AlwNI CAGNNNCTG 1 cut(s) 988
Ama87I CYCGRG 3 cut(s) 626, 842, 1115
AoxI GGCC 3 cut(s) 251, 426, 477
ApaLI GTGCAC 1 cut(s) 694
ApeKI GCWGC 3 cut(s) 47, 701, 1167
ApoI RAATTY 2 cut(s) 239, 1079
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 427, 461
AsuC2I CCSGG 2 cut(s) 627, 628
AsuHPI GGTGA 5 cut(s) 310, 336, 622, 668, 1118
AvaI CYCGRG 3 cut(s) 626, 842, 1115
AvaII GGWCC 1 cut(s) 461
AxyI CCTNAGG 2 cut(s) 378, 711
BaeGI GKGCMC 1 cut(s) 698
BalI TGGCCA 1 cut(s) 253
BauI CACGAG 1 cut(s) 42
Bbv12I GWGCWC 4 cut(s) 43, 160, 698, 775
BbvI GCAGC 3 cut(s) 59, 688, 1154
BccI CCATC 4 cut(s) 283, 347, 492, 868
BceAI ACGGC 1 cut(s) 831
BciT130I CCWGG 1 cut(s) 431
BcnI CCSGG 2 cut(s) 627, 628
BcoDI GTCTC 2 cut(s) 236, 523
BfaI CTAG 3 cut(s) 51, 776, 936
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 3 cut(s) 48, 702, 1168
BlsI GCNGC 3 cut(s) 49, 703, 1169
Bme1390I CCNGG 3 cut(s) 431, 627, 628
Bme18I GGWCC 1 cut(s) 461
BmeT110I CYCGRG 3 cut(s) 626, 842, 1115
BmgT120I GGNCC 2 cut(s) 427, 461
BmrFI CCNGG 3 cut(s) 431, 627, 628
BmsI GCATC 3 cut(s) 500, 958, 1120
BpuMI CCSGG 2 cut(s) 627, 628
BsaAI YACGTR 2 cut(s) 812, 1076
BsaI GGTCTC 2 cut(s) 236, 523
BsaJI CCNNGG 4 cut(s) 429, 626, 843, 1134
BsaWI WCCGGW 1 cut(s) 275
BsaXI ACNNNNNCTCC 2 cut(s) 467, 497
Bsc4I CCNNNNNNNGG 2 cut(s) 353, 1120
Bse1I ACTGG 3 cut(s) 254, 347, 463
Bse21I CCTNAGG 2 cut(s) 378, 711
Bse3DI GCAATG 3 cut(s) 260, 704, 949
BseBI CCWGG 1 cut(s) 431
BseDI CCNNGG 4 cut(s) 429, 626, 843, 1134
BseGI GGATG 2 cut(s) 439, 1184
BseLI CCNNNNNNNGG 2 cut(s) 353, 1120
BseMI GCAATG 3 cut(s) 260, 704, 949
BseMII CTCAG 3 cut(s) 369, 702, 995
BseNI ACTGG 3 cut(s) 254, 347, 463
BseRI GAGGAG 3 cut(s) 395, 488, 728
BseSI GKGCMC 1 cut(s) 698
BseXI GCAGC 3 cut(s) 59, 688, 1154
BsgI GTGCAG 3 cut(s) 66, 1019, 1153
Bsh1236I CGCG 1 cut(s) 724
BshFI GGCC 3 cut(s) 253, 428, 479
BsiHKAI GWGCWC 4 cut(s) 43, 160, 698, 775
BsiHKCI CYCGRG 3 cut(s) 626, 842, 1115
BsiSI CCGG 2 cut(s) 276, 627
BsiWI CGTACG 1 cut(s) 812
BslI CCNNNNNNNGG 2 cut(s) 353, 1120
BsmAI GTCTC 2 cut(s) 236, 523
BsnI GGCC 3 cut(s) 253, 428, 479
Bso31I GGTCTC 2 cut(s) 236, 523
BsoBI CYCGRG 3 cut(s) 626, 842, 1115
Bsp1286I GDGCHC 4 cut(s) 43, 160, 698, 775
Bsp1407I TGTACA 1 cut(s) 559
Bsp143I GATC 3 cut(s) 574, 898, 977
Bsp19I CCATGG 1 cut(s) 1134
BspACI CCGC 3 cut(s) 642, 722, 889
BspANI GGCC 3 cut(s) 253, 428, 479
BspCNI CTCAG 3 cut(s) 370, 703, 994
BspFNI CGCG 1 cut(s) 724
BspPI GGATC 3 cut(s) 569, 893, 985
BspTNI GGTCTC 2 cut(s) 236, 523
BsrDI GCAATG 3 cut(s) 260, 704, 949
BsrGI TGTACA 1 cut(s) 559
BsrI ACTGG 3 cut(s) 254, 347, 463
BssECI CCNNGG 4 cut(s) 429, 626, 843, 1134
BssMI GATC 3 cut(s) 574, 898, 977
BssSI CACGAG 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 1134
Bst2BI CACGAG 1 cut(s) 42
Bst2UI CCWGG 1 cut(s) 431
Bst4CI ACNGT 3 cut(s) 38, 693, 797
Bst6I CTCTTC 1 cut(s) 1045
BstAUI TGTACA 1 cut(s) 559
BstBAI YACGTR 2 cut(s) 812, 1076
BstC8I GCNNGC 1 cut(s) 820
BstDEI CTNAG 5 cut(s) 378, 571, 711, 981, 1022
BstDSI CCRYGG 1 cut(s) 1134
BstF5I GGATG 2 cut(s) 439, 1184
BstFNI CGCG 1 cut(s) 724
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 3 cut(s) 577, 901, 980
BstMAI GTCTC 2 cut(s) 236, 523
BstMBI GATC 3 cut(s) 574, 898, 977
BstMWI GCNNNNNNNGC 2 cut(s) 47, 779
BstNI CCWGG 1 cut(s) 431
BstNSI RCATGY 2 cut(s) 790, 909
BstSCI CCNGG 3 cut(s) 429, 625, 626
BstSLI GKGCMC 1 cut(s) 698
BstUI CGCG 1 cut(s) 724
BstV1I GCAGC 3 cut(s) 59, 688, 1154
BstX2I RGATCY 2 cut(s) 574, 977
BstYI RGATCY 2 cut(s) 574, 977
Bsu36I CCTNAGG 2 cut(s) 378, 711
BsuRI GGCC 3 cut(s) 253, 428, 479
BtgI CCRYGG 1 cut(s) 1134
BtgZI GCGATG 1 cut(s) 887
BtsCI GGATG 2 cut(s) 439, 1184
BtsI GCAGTG 2 cut(s) 696, 775
BtsIMutI CAGTG 4 cut(s) 456, 696, 698, 775
Cac8I GCNNGC 1 cut(s) 820
CaiI CAGNNNCTG 1 cut(s) 988
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 2 cut(s) 427, 461
Cfr9I CCCGGG 1 cut(s) 626
Csp6I GTAC 6 cut(s) 560, 813, 828, 839, 943, 1073
CviAII CATG 6 cut(s) 94, 481, 787, 906, 1012, 1135
CviQI GTAC 6 cut(s) 560, 813, 828, 839, 943, 1073
DdeI CTNAG 5 cut(s) 378, 571, 711, 981, 1022
DpnI GATC 3 cut(s) 576, 900, 979
DpnII GATC 3 cut(s) 574, 898, 977
DraIII CACNNNGTG 1 cut(s) 73
EaeI YGGCCR 1 cut(s) 251
Eam1104I CTCTTC 1 cut(s) 1045
EarI CTCTTC 1 cut(s) 1045
Eco130I CCWWGG 1 cut(s) 1134
Eco31I GGTCTC 2 cut(s) 236, 523
Eco32I GATATC 1 cut(s) 1111
Eco47I GGWCC 1 cut(s) 461
Eco57I CTGAAG 1 cut(s) 900
Eco81I CCTNAGG 2 cut(s) 378, 711
Eco88I CYCGRG 3 cut(s) 626, 842, 1115
EcoRII CCWGG 1 cut(s) 429
EcoRV GATATC 1 cut(s) 1111
EcoT14I CCWWGG 1 cut(s) 1134
EcoT22I ATGCAT 1 cut(s) 515
ErhI CCWWGG 1 cut(s) 1134
FaeI CATG 6 cut(s) 97, 484, 790, 909, 1015, 1138
FatI CATG 6 cut(s) 93, 480, 786, 905, 1011, 1134
FauI CCCGC 1 cut(s) 896
Fnu4HI GCNGC 3 cut(s) 48, 702, 1168
FokI GGATG 2 cut(s) 446, 1171
Fsp4HI GCNGC 3 cut(s) 48, 702, 1168
FspBI CTAG 3 cut(s) 51, 776, 936
GlaI GCGC 1 cut(s) 6
GluI GCNGC 3 cut(s) 48, 702, 1168
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 3 cut(s) 253, 428, 479
HapII CCGG 2 cut(s) 276, 627
HhaI GCGC 1 cut(s) 7
Hin1II CATG 6 cut(s) 97, 484, 790, 909, 1015, 1138
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 129
HindII GTYRAC 1 cut(s) 129
HinfI GANTC 5 cut(s) 272, 383, 613, 668, 1149
HpaII CCGG 2 cut(s) 276, 627
HphI GGTGA 5 cut(s) 310, 336, 622, 668, 1118
Hpy166II GTNNAC 2 cut(s) 129, 696
Hpy188I TCNGA 4 cut(s) 201, 208, 288, 1101
Hpy188III TCNNGA 3 cut(s) 215, 752, 1064
Hpy8I GTNNAC 2 cut(s) 129, 696
HpyAV CCTTC 2 cut(s) 320, 1050
HpyCH4III ACNGT 3 cut(s) 38, 693, 797
HpyCH4IV ACGT 2 cut(s) 811, 1075
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 779
HpyF3I CTNAG 5 cut(s) 378, 571, 711, 981, 1022
HpySE526I ACGT 2 cut(s) 811, 1075
Hsp92II CATG 6 cut(s) 97, 484, 790, 909, 1015, 1138
HspAI GCGC 1 cut(s) 5
Kzo9I GATC 3 cut(s) 574, 898, 977
LmnI GCTCC 2 cut(s) 410, 538
Lsp1109I GCAGC 3 cut(s) 59, 688, 1154
LweI GCATC 3 cut(s) 500, 958, 1120
MaeI CTAG 3 cut(s) 51, 776, 936
MaeII ACGT 2 cut(s) 811, 1075
MaeIII GTNAC 3 cut(s) 331, 342, 958
MalI GATC 3 cut(s) 576, 900, 979
MboI GATC 3 cut(s) 574, 898, 977
MboII GAAGA 5 cut(s) 158, 335, 893, 1032, 1152
MflI RGATCY 2 cut(s) 574, 977
MhlI GDGCHC 4 cut(s) 43, 160, 698, 775
MlsI TGGCCA 1 cut(s) 253
MluCI AATT 6 cut(s) 239, 387, 405, 928, 1000, 1079
MluNI TGGCCA 1 cut(s) 253
MlyI GAGTC 1 cut(s) 392
MmeI TCCRAC 2 cut(s) 82, 224
Mox20I TGGCCA 1 cut(s) 253
Mph1103I ATGCAT 1 cut(s) 515
MscI TGGCCA 1 cut(s) 253
MseI TTAA 3 cut(s) 33, 120, 584
Msp20I TGGCCA 1 cut(s) 253
MspI CCGG 2 cut(s) 276, 627
MspR9I CCNGG 3 cut(s) 431, 627, 628
MvaI CCWGG 1 cut(s) 431
MvnI CGCG 1 cut(s) 724
MwoI GCNNNNNNNGC 2 cut(s) 47, 779
NciI CCSGG 2 cut(s) 627, 628
NcoI CCATGG 1 cut(s) 1134
NdeII GATC 3 cut(s) 574, 898, 977
NlaIII CATG 6 cut(s) 97, 484, 790, 909, 1015, 1138
NmuCI GTSAC 1 cut(s) 342
NsiI ATGCAT 1 cut(s) 515
NspI RCATGY 2 cut(s) 790, 909
PciI ACATGT 2 cut(s) 786, 905
PcsI WCGNNNNNNNCGW 1 cut(s) 493
PfeI GAWTC 4 cut(s) 272, 613, 668, 1149
Pfl23II CGTACG 1 cut(s) 812
PflMI CCANNNNNTGG 1 cut(s) 353
PkrI GCNGC 3 cut(s) 49, 703, 1169
PleI GAGTC 1 cut(s) 391
PpsI GAGTC 1 cut(s) 391
Ppu21I YACGTR 2 cut(s) 812, 1076
PscI ACATGT 2 cut(s) 786, 905
Psp6I CCWGG 1 cut(s) 429
PspGI CCWGG 1 cut(s) 429
PspLI CGTACG 1 cut(s) 812
PspPI GGNCC 2 cut(s) 427, 461
PstNI CAGNNNCTG 1 cut(s) 988
PsuI RGATCY 2 cut(s) 574, 977
RsaI GTAC 6 cut(s) 561, 814, 829, 840, 944, 1074
RsaNI GTAC 6 cut(s) 560, 813, 828, 839, 943, 1073
SaqAI TTAA 3 cut(s) 33, 120, 584
SatI GCNGC 3 cut(s) 48, 702, 1168
Sau3AI GATC 3 cut(s) 574, 898, 977
Sau96I GGNCC 2 cut(s) 427, 461
SchI GAGTC 1 cut(s) 392
ScrFI CCNGG 3 cut(s) 431, 627, 628
SduI GDGCHC 4 cut(s) 43, 160, 698, 775
SfaNI GCATC 3 cut(s) 500, 958, 1120
SinI GGWCC 1 cut(s) 461
SmaI CCCGGG 1 cut(s) 628
Sse9I AATT 6 cut(s) 239, 387, 405, 928, 1000, 1079
SsiI CCGC 3 cut(s) 642, 722, 889
SspMI CTAG 3 cut(s) 51, 776, 936
StyD4I CCNGG 3 cut(s) 429, 625, 626
StyI CCWWGG 1 cut(s) 1134
TaaI ACNGT 3 cut(s) 38, 693, 797
TaiI ACGT 2 cut(s) 814, 1078
TaqI TCGA 3 cut(s) 15, 616, 1152
TasI AATT 6 cut(s) 239, 387, 405, 928, 1000, 1079
TatI WGTACW 1 cut(s) 559
TfiI GAWTC 4 cut(s) 272, 613, 668, 1149
Tru1I TTAA 3 cut(s) 33, 120, 584
Tru9I TTAA 3 cut(s) 33, 120, 584
TscAI CASTG 4 cut(s) 463, 698, 703, 775
TseFI GTSAC 1 cut(s) 342
TseI GCWGC 3 cut(s) 47, 701, 1167
Tsp45I GTSAC 1 cut(s) 342
TspDTI ATGAA 6 cut(s) 82, 260, 660, 938, 1031, 1170
TspGWI ACGGA 2 cut(s) 551, 1164
TspMI CCCGGG 1 cut(s) 626
TspRI CASTG 4 cut(s) 463, 698, 703, 775
Van91I CCANNNNNTGG 1 cut(s) 353
VneI GTGCAC 1 cut(s) 694
VpaK11BI GGWCC 1 cut(s) 461
XapI RAATTY 2 cut(s) 239, 1079
XceI RCATGY 2 cut(s) 790, 909
XcmI CCANNNNNNNNNTGG 1 cut(s) 404
XmaI CCCGGG 1 cut(s) 626
XspI CTAG 3 cut(s) 51, 776, 936
Zsp2I ATGCAT 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.