Rh7AG469700

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
Physical Location & Seq
Forward (+)
65071137 .. 65076360
5224 bp
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UTR
Exon/CDS
Intron
Rh7AG469700.1

Sequence Viewer

Length: 684 bp
ATGGCGCTTCGTTTTGAGGTGTCCTTATTGCATTTAGCTGACATCACTGAGAAGGGTGTAACATTTCAGTCACCAGTCGATGGAAAGCCAATGCTATTGACACCTGAGGAATTAATTCAAATCCAAAATAGAATTGGAGCAGATATTATAATGGCCCTTGATGATGTAGTGAAAACCACCATCACTGGTCCAAGAATTGAGGAGGCCATGTATCGTATTCTACGATGGATTGACAAATGTATAGCAGCTCATACAAGACCGAGCGAGCAGAACTTATTTGGTATTGTTCAAGGTGGCTTAGATCCTGTACTAAGGGATATATGCGTCAGAGGCTTAGTAGAATGGAATTTGCCTGTGTATGCGGTCTTGCGGATGGCGAAAGTAAAGATTCATTTCGGCGCTGCACTGCCTGAGGACAAGCCGCGATATGTTATGGGCGTTGGTTATCCATTGGACATTGTAGTTTGCAGTGCTTTAGGTGCTGACATGTATGACTGTGTTTATCCAACACGTACTGCTCGCTTTGGCACAGCTCTTATACCTGAGGGAGTGCTGAAGCTGAAGCACAAAGCTATGGCTGAGGATATCCGCCCTATCGATCCTACAAGTGCTTGTATGGTATGCAAGAATTATACTAGAGCTTACATTCATTGCCTAGTTACAAAAGATGCTATGGGATCTTAG

Protein Analysis

227

Amino Acids

25.22

Weight (kDa)

6.94

Isoelectric Point (pI)

37.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 7 - 224 9.8e-77 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 149
AccB7I CCANNNNNTGG 1 cut(s) 80
AccII CGCG 1 cut(s) 424
AciI CCGC 4 cut(s) 362, 370, 422, 589
AclWI GGATC 2 cut(s) 296, 593
AcsI RAATTY 1 cut(s) 346
AcuI CTGAAG 2 cut(s) 575, 581
AfaI GTAC 2 cut(s) 309, 514
AfiI CCNNNNNNNGG 1 cut(s) 80
AflIII ACRYGT 2 cut(s) 486, 509
AgsI TTSAA 2 cut(s) 119, 290
AluBI AGCT 6 cut(s) 38, 248, 533, 559, 572, 641
AluI AGCT 6 cut(s) 38, 248, 533, 559, 572, 641
AlwI GGATC 2 cut(s) 296, 593
AoxI GGCC 2 cut(s) 153, 204
ApeKI GCWGC 2 cut(s) 245, 401
ApoI RAATTY 1 cut(s) 346
AseI ATTAAT 1 cut(s) 113
Asp700I GAANNNNTTC 1 cut(s) 114
AspLEI GCGC 2 cut(s) 7, 401
AspS9I GGNCC 2 cut(s) 154, 188
AsuHPI GGTGA 1 cut(s) 63
AvaII GGWCC 1 cut(s) 188
AxyI CCTNAGG 3 cut(s) 105, 411, 543
BbvCI CCTCAGC 1 cut(s) 579
BbvI GCAGC 2 cut(s) 257, 388
BccI CCATC 4 cut(s) 74, 188, 219, 367
BfaI CTAG 2 cut(s) 636, 656
BfoI RGCGCY 2 cut(s) 8, 402
BisI GCNGC 3 cut(s) 246, 402, 422
BlsI GCNGC 3 cut(s) 247, 403, 423
Bme18I GGWCC 1 cut(s) 188
BmgT120I GGNCC 2 cut(s) 154, 188
BmsI GCATC 1 cut(s) 658
Bpu10I CCTNAGC 1 cut(s) 579
Bsa29I ATCGAT 1 cut(s) 597
BsaAI YACGTR 1 cut(s) 512
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 1 cut(s) 80
Bse1I ACTGG 2 cut(s) 74, 190
Bse21I CCTNAGG 3 cut(s) 105, 411, 543
Bse3DI GCAATG 1 cut(s) 649
BseCI ATCGAT 1 cut(s) 597
BseGI GGATG 1 cut(s) 378
BseLI CCNNNNNNNGG 1 cut(s) 80
BseMI GCAATG 1 cut(s) 649
BseMII CTCAG 5 cut(s) 39, 96, 402, 534, 570
BseNI ACTGG 2 cut(s) 74, 190
BseRI GAGGAG 1 cut(s) 215
BseXI GCAGC 2 cut(s) 257, 388
BsgI GTGCAG 1 cut(s) 387
Bsh1236I CGCG 1 cut(s) 424
BshFI GGCC 2 cut(s) 155, 206
BshVI ATCGAT 1 cut(s) 597
BslI CCNNNNNNNGG 1 cut(s) 80
BsnI GGCC 2 cut(s) 155, 206
Bsp143I GATC 3 cut(s) 301, 598, 677
BspACI CCGC 4 cut(s) 362, 370, 422, 589
BspANI GGCC 2 cut(s) 155, 206
BspCNI CTCAG 5 cut(s) 40, 97, 403, 535, 571
BspDI ATCGAT 1 cut(s) 597
BspFNI CGCG 1 cut(s) 424
BspPI GGATC 2 cut(s) 296, 593
BsrDI GCAATG 1 cut(s) 649
BsrI ACTGG 2 cut(s) 74, 190
BssMI GATC 3 cut(s) 301, 598, 677
Bst4CI ACNGT 1 cut(s) 497
BstBAI YACGTR 1 cut(s) 512
BstC8I GCNNGC 2 cut(s) 266, 520
BstDEI CTNAG 9 cut(s) 48, 105, 298, 311, 334, 411, 543, 579, 681
BstF5I GGATG 1 cut(s) 378
BstFNI CGCG 1 cut(s) 424
BstH2I RGCGCY 2 cut(s) 8, 402
BstHHI GCGC 2 cut(s) 7, 401
BstKTI GATC 3 cut(s) 304, 601, 680
BstMBI GATC 3 cut(s) 301, 598, 677
BstMWI GCNNNNNNNGC 2 cut(s) 330, 479
BstNSI RCATGY 1 cut(s) 490
BstUI CGCG 1 cut(s) 424
BstV1I GCAGC 2 cut(s) 257, 388
BstX2I RGATCY 2 cut(s) 301, 677
BstYI RGATCY 2 cut(s) 301, 677
Bsu15I ATCGAT 1 cut(s) 597
Bsu36I CCTNAGG 3 cut(s) 105, 411, 543
BsuRI GGCC 2 cut(s) 155, 206
BsuTUI ATCGAT 1 cut(s) 597
BtsCI GGATG 1 cut(s) 378
BtsI GCAGTG 2 cut(s) 404, 475
BtsIMutI CAGTG 4 cut(s) 45, 183, 404, 475
Cac8I GCNNGC 2 cut(s) 266, 520
CfoI GCGC 2 cut(s) 7, 401
Cfr13I GGNCC 2 cut(s) 154, 188
ClaI ATCGAT 1 cut(s) 597
CseI GACGC 1 cut(s) 313
Csp6I GTAC 2 cut(s) 308, 513
CviAII CATG 2 cut(s) 208, 487
CviQI GTAC 2 cut(s) 308, 513
DdeI CTNAG 9 cut(s) 48, 105, 298, 311, 334, 411, 543, 579, 681
DpnI GATC 3 cut(s) 303, 600, 679
DpnII GATC 3 cut(s) 301, 598, 677
EciI GGCGGA 1 cut(s) 578
Eco32I GATATC 1 cut(s) 586
Eco47I GGWCC 1 cut(s) 188
Eco57I CTGAAG 2 cut(s) 575, 581
Eco81I CCTNAGG 3 cut(s) 105, 411, 543
EcoRV GATATC 1 cut(s) 586
FaeI CATG 2 cut(s) 211, 490
FatI CATG 2 cut(s) 207, 486
Fnu4HI GCNGC 3 cut(s) 246, 402, 422
FokI GGATG 1 cut(s) 385
Fsp4HI GCNGC 3 cut(s) 246, 402, 422
FspBI CTAG 2 cut(s) 636, 656
GlaI GCGC 2 cut(s) 6, 400
GluI GCNGC 3 cut(s) 246, 402, 422
HaeII RGCGCY 2 cut(s) 8, 402
HaeIII GGCC 2 cut(s) 155, 206
HgaI GACGC 1 cut(s) 313
HhaI GCGC 2 cut(s) 7, 401
Hin1II CATG 2 cut(s) 211, 490
Hin6I GCGC 2 cut(s) 5, 399
HinP1I GCGC 2 cut(s) 5, 399
HinfI GANTC 1 cut(s) 388
HphI GGTGA 1 cut(s) 63
Hpy188I TCNGA 1 cut(s) 329
HpyAV CCTTC 1 cut(s) 46
HpyCH4III ACNGT 1 cut(s) 497
HpyCH4IV ACGT 1 cut(s) 511
HpyCH4V TGCA 4 cut(s) 31, 404, 468, 624
HpyF10VI GCNNNNNNNGC 2 cut(s) 330, 479
HpyF3I CTNAG 9 cut(s) 48, 105, 298, 311, 334, 411, 543, 579, 681
HpySE526I ACGT 1 cut(s) 511
Hsp92II CATG 2 cut(s) 211, 490
HspAI GCGC 2 cut(s) 5, 399
Kzo9I GATC 3 cut(s) 301, 598, 677
LmnI GCTCC 1 cut(s) 137
LpnPI CCDG 7 cut(s) 87, 117, 171, 318, 366, 423, 555
Lsp1109I GCAGC 2 cut(s) 257, 388
LweI GCATC 1 cut(s) 658
MaeI CTAG 2 cut(s) 636, 656
MaeII ACGT 1 cut(s) 511
MaeIII GTNAC 3 cut(s) 58, 69, 658
MalI GATC 3 cut(s) 303, 600, 679
MboI GATC 3 cut(s) 301, 598, 677
MflI RGATCY 2 cut(s) 301, 677
MluCI AATT 6 cut(s) 110, 114, 132, 195, 346, 628
MmeI TCCRAC 1 cut(s) 530
MnlI CCTC 8 cut(s) 10, 100, 193, 196, 323, 406, 538, 574
MroXI GAANNNNTTC 1 cut(s) 114
MseI TTAA 1 cut(s) 113
MvnI CGCG 1 cut(s) 424
MwoI GCNNNNNNNGC 2 cut(s) 330, 479
NdeII GATC 3 cut(s) 301, 598, 677
NlaIII CATG 2 cut(s) 211, 490
NmuCI GTSAC 1 cut(s) 69
NspI RCATGY 1 cut(s) 490
PciI ACATGT 1 cut(s) 486
PcsI WCGNNNNNNNCGW 1 cut(s) 220
PdmI GAANNNNTTC 1 cut(s) 114
PfeI GAWTC 1 cut(s) 388
PflMI CCANNNNNTGG 1 cut(s) 80
PkrI GCNGC 3 cut(s) 247, 403, 423
Ppu21I YACGTR 1 cut(s) 512
PscI ACATGT 1 cut(s) 486
PshBI ATTAAT 1 cut(s) 113
PsiI TTATAA 1 cut(s) 149
PspPI GGNCC 2 cut(s) 154, 188
PsuI RGATCY 2 cut(s) 301, 677
RsaI GTAC 2 cut(s) 309, 514
RsaNI GTAC 2 cut(s) 308, 513
SaqAI TTAA 1 cut(s) 113
SatI GCNGC 3 cut(s) 246, 402, 422
Sau3AI GATC 3 cut(s) 301, 598, 677
Sau96I GGNCC 2 cut(s) 154, 188
SfaNI GCATC 1 cut(s) 658
SinI GGWCC 1 cut(s) 188
Sse9I AATT 6 cut(s) 110, 114, 132, 195, 346, 628
SsiI CCGC 4 cut(s) 362, 370, 422, 589
SspMI CTAG 2 cut(s) 636, 656
TaaI ACNGT 1 cut(s) 497
TaiI ACGT 1 cut(s) 514
TaqI TCGA 2 cut(s) 78, 597
TaqII GACCGA 1 cut(s) 274
TasI AATT 6 cut(s) 110, 114, 132, 195, 346, 628
TatI WGTACW 1 cut(s) 307
TauI GCSGC 1 cut(s) 424
TfiI GAWTC 1 cut(s) 388
Tru1I TTAA 1 cut(s) 113
Tru9I TTAA 1 cut(s) 113
TscAI CASTG 4 cut(s) 52, 190, 411, 475
TseFI GTSAC 1 cut(s) 69
TseI GCWGC 2 cut(s) 245, 401
Tsp45I GTSAC 1 cut(s) 69
TspDTI ATGAA 2 cut(s) 380, 638
TspRI CASTG 4 cut(s) 52, 190, 411, 475
Van91I CCANNNNNTGG 1 cut(s) 80
VpaK11BI GGWCC 1 cut(s) 188
VspI ATTAAT 1 cut(s) 113
XapI RAATTY 1 cut(s) 346
XceI RCATGY 1 cut(s) 490
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
XmnI GAANNNNTTC 1 cut(s) 114
XspI CTAG 2 cut(s) 636, 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.