Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_5g36560 FvH4_5g36560 FvH4_5g36560 FvH4_5g36560 FvH4_5g36560 FvH4_5g36560 |
| malus_domestica | MD08G1188600.v1.1 MD15G1376400.v1.1 |
| prunus_persica | Prupe.1G523100_v2.0.a1 Prupe.1G523100_v2.0.a1 |
| pyrus_communis | pycom08g16230 pycom15g33630 |
| rosa_chinensis | RchiOBHm_Chr3g0492071 RchiOBHm_Chr7g0242241 |
| rosa_laevigata | RLG00000000904 RLG00000022795 |
| rosa_multiflora | Rmu_sc0003846.1_g000008 Rmu_sc0031707.1_g000001 |
| rosa_roxburghii | Rroxscaffold_6G00392560 |
| rosa_rugosa | Rorug03G0251600 Rorug03G0251700 Rorug07G0314300 |
| rosa_samantha | Rh3AG301800 Rh3BG337600 Rh3BG337700 Rh3CG335200 Rh3DG337300 Rh7AG469700 Rh7BG439500 Rh7CG486300 Rh7DG454700 |
| rosa_wichuraiana | Rw3G026640 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 353 |
| AccII | CGCG | 2 cut(s) | 682, 801 |
| AciI | CCGC | 5 cut(s) | 600, 612, 660, 680, 847 |
| AclWI | GGATC | 4 cut(s) | 492, 569, 851, 943 |
| AcoI | YGGCCR | 1 cut(s) | 251 |
| AcsI | RAATTY | 1 cut(s) | 239 |
| AcuI | CTGAAG | 1 cut(s) | 858 |
| AdeI | CACNNNGTG | 1 cut(s) | 73 |
| AfaI | GTAC | 4 cut(s) | 561, 772, 787, 798 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 353, 1003 |
| AflIII | ACRYGT | 3 cut(s) | 744, 767, 863 |
| AgsI | TTSAA | 2 cut(s) | 146, 392 |
| AluBI | AGCT | 8 cut(s) | 50, 142, 211, 311, 521, 791, 899, 943 |
| AluI | AGCT | 8 cut(s) | 50, 142, 211, 311, 521, 791, 899, 943 |
| Alw21I | GWGCWC | 4 cut(s) | 43, 160, 656, 733 |
| Alw26I | GTCTC | 2 cut(s) | 236, 523 |
| Alw44I | GTGCAC | 1 cut(s) | 652 |
| AlwI | GGATC | 4 cut(s) | 492, 569, 851, 943 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 946 |
| Ama87I | CYCGRG | 1 cut(s) | 998 |
| AoxI | GGCC | 3 cut(s) | 251, 426, 477 |
| ApaLI | GTGCAC | 1 cut(s) | 652 |
| ApeKI | GCWGC | 2 cut(s) | 47, 1050 |
| ApoI | RAATTY | 1 cut(s) | 239 |
| AspLEI | GCGC | 1 cut(s) | 7 |
| AspS9I | GGNCC | 2 cut(s) | 427, 461 |
| AsuHPI | GGTGA | 4 cut(s) | 310, 336, 626, 1001 |
| AvaI | CYCGRG | 1 cut(s) | 998 |
| AvaII | GGWCC | 1 cut(s) | 461 |
| AxyI | CCTNAGG | 2 cut(s) | 378, 669 |
| BaeGI | GKGCMC | 1 cut(s) | 656 |
| BalI | TGGCCA | 1 cut(s) | 253 |
| BauI | CACGAG | 1 cut(s) | 42 |
| Bbv12I | GWGCWC | 4 cut(s) | 43, 160, 656, 733 |
| BbvI | GCAGC | 2 cut(s) | 59, 1037 |
| BccI | CCATC | 4 cut(s) | 283, 347, 492, 826 |
| BcoDI | GTCTC | 2 cut(s) | 236, 523 |
| BfaI | CTAG | 3 cut(s) | 51, 734, 894 |
| BfoI | RGCGCY | 1 cut(s) | 8 |
| BisI | GCNGC | 3 cut(s) | 48, 660, 1051 |
| BlsI | GCNGC | 3 cut(s) | 49, 661, 1052 |
| Bme18I | GGWCC | 1 cut(s) | 461 |
| BmeT110I | CYCGRG | 1 cut(s) | 998 |
| BmgT120I | GGNCC | 2 cut(s) | 427, 461 |
| BmsI | GCATC | 3 cut(s) | 500, 916, 1003 |
| BsaAI | YACGTR | 2 cut(s) | 180, 770 |
| BsaI | GGTCTC | 2 cut(s) | 236, 523 |
| BsaJI | CCNNGG | 2 cut(s) | 480, 1017 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 353, 1003 |
| Bse1I | ACTGG | 3 cut(s) | 254, 347, 463 |
| Bse21I | CCTNAGG | 2 cut(s) | 378, 669 |
| Bse3DI | GCAATG | 3 cut(s) | 260, 662, 907 |
| BseDI | CCNNGG | 2 cut(s) | 480, 1017 |
| BseGI | GGATG | 1 cut(s) | 1067 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 353, 1003 |
| BseMI | GCAATG | 3 cut(s) | 260, 662, 907 |
| BseMII | CTCAG | 4 cut(s) | 198, 369, 660, 953 |
| BseNI | ACTGG | 3 cut(s) | 254, 347, 463 |
| BseRI | GAGGAG | 2 cut(s) | 395, 686 |
| BseSI | GKGCMC | 1 cut(s) | 656 |
| BseXI | GCAGC | 2 cut(s) | 59, 1037 |
| BsgI | GTGCAG | 2 cut(s) | 66, 1036 |
| Bsh1236I | CGCG | 2 cut(s) | 682, 801 |
| BshFI | GGCC | 3 cut(s) | 253, 428, 479 |
| BsiHKAI | GWGCWC | 4 cut(s) | 43, 160, 656, 733 |
| BsiHKCI | CYCGRG | 1 cut(s) | 998 |
| BslFI | GGGAC | 1 cut(s) | 980 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 353, 1003 |
| BsmAI | GTCTC | 2 cut(s) | 236, 523 |
| BsmFI | GGGAC | 1 cut(s) | 980 |
| BsnI | GGCC | 3 cut(s) | 253, 428, 479 |
| Bso31I | GGTCTC | 2 cut(s) | 236, 523 |
| BsoBI | CYCGRG | 1 cut(s) | 998 |
| Bsp1286I | GDGCHC | 4 cut(s) | 43, 160, 656, 733 |
| Bsp1407I | TGTACA | 1 cut(s) | 559 |
| Bsp143I | GATC | 4 cut(s) | 484, 574, 856, 935 |
| Bsp19I | CCATGG | 2 cut(s) | 480, 1017 |
| BspACI | CCGC | 5 cut(s) | 600, 612, 660, 680, 847 |
| BspANI | GGCC | 3 cut(s) | 253, 428, 479 |
| BspCNI | CTCAG | 4 cut(s) | 199, 370, 661, 952 |
| BspFNI | CGCG | 2 cut(s) | 682, 801 |
| BspPI | GGATC | 4 cut(s) | 492, 569, 851, 943 |
| BspTNI | GGTCTC | 2 cut(s) | 236, 523 |
| BsrDI | GCAATG | 3 cut(s) | 260, 662, 907 |
| BsrGI | TGTACA | 1 cut(s) | 559 |
| BsrI | ACTGG | 3 cut(s) | 254, 347, 463 |
| BssECI | CCNNGG | 2 cut(s) | 480, 1017 |
| BssMI | GATC | 4 cut(s) | 484, 574, 856, 935 |
| BssSI | CACGAG | 1 cut(s) | 42 |
| BssT1I | CCWWGG | 2 cut(s) | 480, 1017 |
| Bst2BI | CACGAG | 1 cut(s) | 42 |
| Bst4CI | ACNGT | 3 cut(s) | 38, 651, 755 |
| BstAUI | TGTACA | 1 cut(s) | 559 |
| BstBAI | YACGTR | 2 cut(s) | 180, 770 |
| BstC8I | GCNNGC | 1 cut(s) | 778 |
| BstDEI | CTNAG | 5 cut(s) | 207, 378, 571, 669, 939 |
| BstDSI | CCRYGG | 2 cut(s) | 480, 1017 |
| BstF5I | GGATG | 1 cut(s) | 1067 |
| BstFNI | CGCG | 2 cut(s) | 682, 801 |
| BstH2I | RGCGCY | 1 cut(s) | 8 |
| BstHHI | GCGC | 1 cut(s) | 7 |
| BstKTI | GATC | 4 cut(s) | 487, 577, 859, 938 |
| BstMAI | GTCTC | 2 cut(s) | 236, 523 |
| BstMBI | GATC | 4 cut(s) | 484, 574, 856, 935 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 47, 737 |
| BstNSI | RCATGY | 2 cut(s) | 748, 867 |
| BstSLI | GKGCMC | 1 cut(s) | 656 |
| BstSNI | TACGTA | 1 cut(s) | 180 |
| BstUI | CGCG | 2 cut(s) | 682, 801 |
| BstV1I | GCAGC | 2 cut(s) | 59, 1037 |
| BstX2I | RGATCY | 2 cut(s) | 574, 935 |
| BstYI | RGATCY | 2 cut(s) | 574, 935 |
| Bsu36I | CCTNAGG | 2 cut(s) | 378, 669 |
| BsuRI | GGCC | 3 cut(s) | 253, 428, 479 |
| BtgI | CCRYGG | 2 cut(s) | 480, 1017 |
| BtgZI | GCGATG | 1 cut(s) | 845 |
| BtsCI | GGATG | 1 cut(s) | 1067 |
| BtsI | GCAGTG | 2 cut(s) | 654, 733 |
| BtsIMutI | CAGTG | 4 cut(s) | 456, 654, 656, 733 |
| Cac8I | GCNNGC | 1 cut(s) | 778 |
| CaiI | CAGNNNCTG | 1 cut(s) | 946 |
| CfoI | GCGC | 1 cut(s) | 7 |
| Cfr13I | GGNCC | 2 cut(s) | 427, 461 |
| Csp6I | GTAC | 4 cut(s) | 560, 771, 786, 797 |
| CviAII | CATG | 6 cut(s) | 94, 481, 745, 864, 970, 1018 |
| CviQI | GTAC | 4 cut(s) | 560, 771, 786, 797 |
| DdeI | CTNAG | 5 cut(s) | 207, 378, 571, 669, 939 |
| DpnI | GATC | 4 cut(s) | 486, 576, 858, 937 |
| DpnII | GATC | 4 cut(s) | 484, 574, 856, 935 |
| DraIII | CACNNNGTG | 1 cut(s) | 73 |
| EaeI | YGGCCR | 1 cut(s) | 251 |
| Eco105I | TACGTA | 1 cut(s) | 180 |
| Eco130I | CCWWGG | 2 cut(s) | 480, 1017 |
| Eco31I | GGTCTC | 2 cut(s) | 236, 523 |
| Eco47I | GGWCC | 1 cut(s) | 461 |
| Eco57I | CTGAAG | 1 cut(s) | 858 |
| Eco81I | CCTNAGG | 2 cut(s) | 378, 669 |
| Eco88I | CYCGRG | 1 cut(s) | 998 |
| EcoT14I | CCWWGG | 2 cut(s) | 480, 1017 |
| EcoT22I | ATGCAT | 1 cut(s) | 515 |
| ErhI | CCWWGG | 2 cut(s) | 480, 1017 |
| FaeI | CATG | 6 cut(s) | 97, 484, 748, 867, 973, 1021 |
| FaqI | GGGAC | 1 cut(s) | 980 |
| FatI | CATG | 6 cut(s) | 93, 480, 744, 863, 969, 1017 |
| FauI | CCCGC | 1 cut(s) | 854 |
| Fnu4HI | GCNGC | 3 cut(s) | 48, 660, 1051 |
| FokI | GGATG | 1 cut(s) | 1054 |
| Fsp4HI | GCNGC | 3 cut(s) | 48, 660, 1051 |
| FspBI | CTAG | 3 cut(s) | 51, 734, 894 |
| GlaI | GCGC | 1 cut(s) | 6 |
| GluI | GCNGC | 3 cut(s) | 48, 660, 1051 |
| HaeII | RGCGCY | 1 cut(s) | 8 |
| HaeIII | GGCC | 3 cut(s) | 253, 428, 479 |
| HhaI | GCGC | 1 cut(s) | 7 |
| Hin1II | CATG | 6 cut(s) | 97, 484, 748, 867, 973, 1021 |
| Hin6I | GCGC | 1 cut(s) | 5 |
| HinP1I | GCGC | 1 cut(s) | 5 |
| HincII | GTYRAC | 1 cut(s) | 129 |
| HindII | GTYRAC | 1 cut(s) | 129 |
| HinfI | GANTC | 4 cut(s) | 272, 383, 626, 1032 |
| HphI | GGTGA | 4 cut(s) | 310, 336, 626, 1001 |
| Hpy166II | GTNNAC | 2 cut(s) | 129, 654 |
| Hpy188I | TCNGA | 4 cut(s) | 201, 208, 288, 984 |
| Hpy188III | TCNNGA | 2 cut(s) | 215, 710 |
| Hpy8I | GTNNAC | 2 cut(s) | 129, 654 |
| HpyAV | CCTTC | 1 cut(s) | 320 |
| HpyCH4III | ACNGT | 3 cut(s) | 38, 651, 755 |
| HpyCH4IV | ACGT | 2 cut(s) | 179, 769 |
| HpyCH4V | TGCA | 8 cut(s) | 47, 304, 513, 647, 654, 726, 882, 1053 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 47, 737 |
| HpyF3I | CTNAG | 5 cut(s) | 207, 378, 571, 669, 939 |
| HpySE526I | ACGT | 2 cut(s) | 179, 769 |
| Hsp92II | CATG | 6 cut(s) | 97, 484, 748, 867, 973, 1021 |
| HspAI | GCGC | 1 cut(s) | 5 |
| Kzo9I | GATC | 4 cut(s) | 484, 574, 856, 935 |
| LmnI | GCTCC | 1 cut(s) | 410 |
| Lsp1109I | GCAGC | 2 cut(s) | 59, 1037 |
| LweI | GCATC | 3 cut(s) | 500, 916, 1003 |
| MaeI | CTAG | 3 cut(s) | 51, 734, 894 |
| MaeII | ACGT | 2 cut(s) | 179, 769 |
| MaeIII | GTNAC | 3 cut(s) | 331, 342, 916 |
| MalI | GATC | 4 cut(s) | 486, 576, 858, 937 |
| MboI | GATC | 4 cut(s) | 484, 574, 856, 935 |
| MboII | GAAGA | 4 cut(s) | 158, 335, 851, 1035 |
| MflI | RGATCY | 2 cut(s) | 574, 935 |
| MhlI | GDGCHC | 4 cut(s) | 43, 160, 656, 733 |
| MlsI | TGGCCA | 1 cut(s) | 253 |
| MluCI | AATT | 5 cut(s) | 239, 387, 405, 886, 958 |
| MluNI | TGGCCA | 1 cut(s) | 253 |
| MlyI | GAGTC | 1 cut(s) | 392 |
| MmeI | TCCRAC | 2 cut(s) | 82, 224 |
| Mox20I | TGGCCA | 1 cut(s) | 253 |
| Mph1103I | ATGCAT | 1 cut(s) | 515 |
| MscI | TGGCCA | 1 cut(s) | 253 |
| MseI | TTAA | 4 cut(s) | 33, 471, 516, 584 |
| Msp20I | TGGCCA | 1 cut(s) | 253 |
| MvnI | CGCG | 2 cut(s) | 682, 801 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 47, 737 |
| NcoI | CCATGG | 2 cut(s) | 480, 1017 |
| NdeII | GATC | 4 cut(s) | 484, 574, 856, 935 |
| NlaIII | CATG | 6 cut(s) | 97, 484, 748, 867, 973, 1021 |
| NmuCI | GTSAC | 1 cut(s) | 342 |
| NsiI | ATGCAT | 1 cut(s) | 515 |
| NspI | RCATGY | 2 cut(s) | 748, 867 |
| PciI | ACATGT | 2 cut(s) | 744, 863 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 493 |
| PfeI | GAWTC | 3 cut(s) | 272, 626, 1032 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 353 |
| PkrI | GCNGC | 3 cut(s) | 49, 661, 1052 |
| PleI | GAGTC | 1 cut(s) | 391 |
| PpsI | GAGTC | 1 cut(s) | 391 |
| Ppu21I | YACGTR | 2 cut(s) | 180, 770 |
| PscI | ACATGT | 2 cut(s) | 744, 863 |
| PspPI | GGNCC | 2 cut(s) | 427, 461 |
| PstNI | CAGNNNCTG | 1 cut(s) | 946 |
| PsuI | RGATCY | 2 cut(s) | 574, 935 |
| RsaI | GTAC | 4 cut(s) | 561, 772, 787, 798 |
| RsaNI | GTAC | 4 cut(s) | 560, 771, 786, 797 |
| SaqAI | TTAA | 4 cut(s) | 33, 471, 516, 584 |
| SatI | GCNGC | 3 cut(s) | 48, 660, 1051 |
| Sau3AI | GATC | 4 cut(s) | 484, 574, 856, 935 |
| Sau96I | GGNCC | 2 cut(s) | 427, 461 |
| SchI | GAGTC | 1 cut(s) | 392 |
| SduI | GDGCHC | 4 cut(s) | 43, 160, 656, 733 |
| SfaNI | GCATC | 3 cut(s) | 500, 916, 1003 |
| SinI | GGWCC | 1 cut(s) | 461 |
| SnaBI | TACGTA | 1 cut(s) | 180 |
| Sse9I | AATT | 5 cut(s) | 239, 387, 405, 886, 958 |
| SsiI | CCGC | 5 cut(s) | 600, 612, 660, 680, 847 |
| SspMI | CTAG | 3 cut(s) | 51, 734, 894 |
| StyI | CCWWGG | 2 cut(s) | 480, 1017 |
| TaaI | ACNGT | 3 cut(s) | 38, 651, 755 |
| TaiI | ACGT | 2 cut(s) | 182, 772 |
| TaqI | TCGA | 3 cut(s) | 15, 432, 1035 |
| TasI | AATT | 5 cut(s) | 239, 387, 405, 886, 958 |
| TatI | WGTACW | 1 cut(s) | 559 |
| TauI | GCSGC | 1 cut(s) | 662 |
| TfiI | GAWTC | 3 cut(s) | 272, 626, 1032 |
| Tru1I | TTAA | 4 cut(s) | 33, 471, 516, 584 |
| Tru9I | TTAA | 4 cut(s) | 33, 471, 516, 584 |
| TscAI | CASTG | 4 cut(s) | 463, 656, 661, 733 |
| TseFI | GTSAC | 1 cut(s) | 342 |
| TseI | GCWGC | 2 cut(s) | 47, 1050 |
| Tsp45I | GTSAC | 1 cut(s) | 342 |
| TspDTI | ATGAA | 6 cut(s) | 82, 260, 618, 896, 989, 1053 |
| TspRI | CASTG | 4 cut(s) | 463, 656, 661, 733 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 353 |
| VneI | GTGCAC | 1 cut(s) | 652 |
| VpaK11BI | GGWCC | 1 cut(s) | 461 |
| XapI | RAATTY | 1 cut(s) | 239 |
| XceI | RCATGY | 2 cut(s) | 748, 867 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 404 |
| XspI | CTAG | 3 cut(s) | 51, 734, 894 |
| Zsp2I | ATGCAT | 1 cut(s) | 515 |