Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0004542

Physical Location & Seq

Genomic Coordinates Reverse (-)

12147876 .. 12156398

Gene Structure 8523 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00392560.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1203 bp

ATGGCGCTTCGTTTCGAGGTTTTGGGGAGGTTCAATCGTGCTCGTGCAGCTCAACTCACACTCCCTCACTTTGTATGTCAGACTCCTCTTTTCATGCCTGTTGGAACACAAGGAACTATAAAAGGGTTGGCAACTGACCAGCTTGAGGAGATTGGTTGCCAAATTATTCTTGGGAATACATATCATTTGGCACTTAGACCTACTTCTGAGCTTATTGACGAGATGGGAGGTCTCCACAAATTTATGAATTGGCCAAGAGCGCTGCTTACTGATTCTGGTGGCTTTCAGATGGTATCCTTATTGCATTTAGCTGACATCACTGAGGAGGGTGTAACATTTCAGTCACCGGTCGATGGAAAGCCAATGCTCTTGACACCTGAGGAATCAATTCAAGTCCAAAATAGAATTGGAGCAGATATTATAATGGCCCTTGATGATGTAGTGAGAACCACCATCACTGGTCCAAGAATTGAGGAGGCCATGTATCGTACTCTACGATGGATTGACAGATGTATAGCAGCTCATACAAGACCGAGCGAGCAGAACTTATTTGGTATTGTTCAAGGTGGCTTAGATCCTGTACTAAGGGATATATGCGTCAGAGGCTTAGTAGAACGGAATTTGCCTGGCTATGCCATAGGCGGTCTTGCGGGTGGCGAAAGTAAGGATTCATTTTGGCGTGTTGTTGCTCAGTGCACTGCTGCACTGCCTGAGGACAAGCCGCGATATGTTATGGGCGTTGGTTATCCATTGGACATTGTAGTTTGCAGTGCTTTAGGTGCTGACATGTATGACTGTGTTTATCCAACACGTACTGCTCGCTTTGGCACTGCTCTTATACCTGAGGGAGTGCTGAAGCTGAAGCACAAAGCTATGGCTGAGGATACCCGCCCTATCGATCGTACATGTGCTTGTATGGTGTGCAAGAATTATACTAGAGCTTACATTCATTGCCTAGTTACAAAAGATGCTATGGGATCTCAGCTTCTGTCATATCACAACCTTTATTACATGCTAAAGCTAAGTAGAGATCTACACTCATCAATAATCCAAGGAACATTTCCAGAGTTTATATGCAACTTTCTGCAAACAATGTTTCCTAAAGGAGATGTCCCGGAATGGGTCTGCAATGCCATGGAGGTTGCTGGAATTGACATCTCTTCCTGCTGTACTCCATTTTCTTCATCCCAAGATTCAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0008479 GO:0009451 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016740 GO:0016757 GO:0016763 GO:0019867 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0046982 GO:0046983 GO:0071704 GO:0090304 GO:0098588 GO:0098805 GO:0101030 GO:0140098 GO:0140101 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TGT

Protein Analysis

400

Amino Acids

44.28

Weight (kDa)

5.64

Isoelectric Point (pI)

42.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TGT	PF01702	13 - 365	4.4e-152	Queuine tRNA-ribosyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004542)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g36560 FvH4_5g36560 FvH4_5g36560 FvH4_5g36560 FvH4_5g36560 FvH4_5g36560
malus_domestica	MD08G1188600.v1.1 MD15G1376400.v1.1
prunus_persica	Prupe.1G523100_v2.0.a1 Prupe.1G523100_v2.0.a1
pyrus_communis	pycom08g16230 pycom15g33630
rosa_chinensis	RchiOBHm_Chr3g0492071 RchiOBHm_Chr7g0242241
rosa_laevigata	RLG00000000904 RLG00000022795
rosa_multiflora	Rmu_sc0003846.1_g000008 Rmu_sc0031707.1_g000001
rosa_roxburghii	Rroxscaffold_6G00392560
rosa_rugosa	Rorug03G0251600 Rorug03G0251700 Rorug07G0314300
rosa_samantha	Rh3AG301800 Rh3BG337600 Rh3BG337700 Rh3CG335200 Rh3DG337300 Rh7AG469700 Rh7BG439500 Rh7CG486300 Rh7DG454700
rosa_wichuraiana	Rw3G026640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	422
AccII	CGCG	1 cut(s)	724
AciI	CCGC	4 cut(s)	642, 650, 722, 889
AclWI	GGATC	2 cut(s)	569, 985
AcoI	YGGCCR	1 cut(s)	251
AcsI	RAATTY	2 cut(s)	239, 619
AcuI	CTGAAG	2 cut(s)	875, 881
AfaI	GTAC	5 cut(s)	490, 582, 814, 904, 1171
AfeI	AGCGCT	1 cut(s)	261
AfiI	CCNNNNNNNGG	3 cut(s)	145, 353, 1120
AflIII	ACRYGT	3 cut(s)	786, 809, 905
AgeI	ACCGGT	1 cut(s)	346
AgsI	TTSAA	3 cut(s)	34, 392, 563
AjnI	CCWGG	1 cut(s)	625
AjuI	GAANNNNNNNTTGG	2 cut(s)	1044, 1076
Alw21I	GWGCWC	2 cut(s)	43, 698
Alw26I	GTCTC	1 cut(s)	236
Alw44I	GTGCAC	1 cut(s)	694
AlwI	GGATC	2 cut(s)	569, 985
AlwNI	CAGNNNCTG	1 cut(s)	988
Aor51HI	AGCGCT	1 cut(s)	261
AoxI	GGCC	3 cut(s)	251, 426, 477
ApaLI	GTGCAC	1 cut(s)	694
ApeKI	GCWGC	4 cut(s)	47, 262, 518, 701
ApoI	RAATTY	2 cut(s)	239, 619
AsiGI	ACCGGT	1 cut(s)	346
Asp700I	GAANNNNTTC	1 cut(s)	387
AspLEI	GCGC	2 cut(s)	7, 262
AspS9I	GGNCC	2 cut(s)	427, 461
AsuC2I	CCSGG	1 cut(s)	1115
AsuHPI	GGTGA	1 cut(s)	336
AvaII	GGWCC	1 cut(s)	461
AxyI	CCTNAGG	3 cut(s)	378, 711, 843
BaeGI	GKGCMC	1 cut(s)	698
BalI	TGGCCA	1 cut(s)	253
BauI	CACGAG	1 cut(s)	42
Bbv12I	GWGCWC	2 cut(s)	43, 698
BbvCI	CCTCAGC	1 cut(s)	879
BbvI	GCAGC	4 cut(s)	59, 249, 530, 688
BccI	CCATC	5 cut(s)	217, 283, 347, 461, 492
BciT130I	CCWGG	1 cut(s)	627
BciVI	GTATCC	2 cut(s)	304, 877
BcnI	CCSGG	1 cut(s)	1115
BcoDI	GTCTC	1 cut(s)	236
BfaI	CTAG	2 cut(s)	936, 956
BfoI	RGCGCY	2 cut(s)	8, 263
BfuI	GTATCC	2 cut(s)	304, 877
BglII	AGATCT	1 cut(s)	1030
BisI	GCNGC	5 cut(s)	48, 263, 519, 702, 722
BlsI	GCNGC	5 cut(s)	49, 264, 520, 703, 723
Bme1390I	CCNGG	2 cut(s)	627, 1115
Bme18I	GGWCC	1 cut(s)	461
BmgT120I	GGNCC	2 cut(s)	427, 461
BmrFI	CCNGG	2 cut(s)	627, 1115
BmsI	GCATC	1 cut(s)	958
Bpu10I	CCTNAGC	1 cut(s)	879
BpuEI	CTTGAG	1 cut(s)	164
BpuMI	CCSGG	1 cut(s)	1115
Bsa29I	ATCGAT	1 cut(s)	897
BsaAI	YACGTR	1 cut(s)	812
BsaI	GGTCTC	1 cut(s)	236
BsaJI	CCNNGG	2 cut(s)	1051, 1134
BsaWI	WCCGGW	1 cut(s)	346
BsaXI	ACNNNNNCTCC	4 cut(s)	45, 75, 467, 497
Bsc4I	CCNNNNNNNGG	3 cut(s)	145, 353, 1120
Bse118I	RCCGGY	1 cut(s)	346
Bse1I	ACTGG	1 cut(s)	463
Bse21I	CCTNAGG	3 cut(s)	378, 711, 843
Bse3DI	GCAATG	2 cut(s)	949, 1135
BseBI	CCWGG	1 cut(s)	627
BseCI	ATCGAT	1 cut(s)	897
BseDI	CCNNGG	2 cut(s)	1051, 1134
BseGI	GGATG	1 cut(s)	1184
BseLI	CCNNNNNNNGG	3 cut(s)	145, 353, 1120
BseMI	GCAATG	2 cut(s)	949, 1135
BseMII	CTCAG	8 cut(s)	198, 312, 369, 702, 704, 834, 870, 995
BseNI	ACTGG	1 cut(s)	463
BseRI	GAGGAG	4 cut(s)	75, 161, 338, 488
BseSI	GKGCMC	1 cut(s)	698
BseXI	GCAGC	4 cut(s)	59, 249, 530, 688
BsgI	GTGCAG	2 cut(s)	66, 687
Bsh1236I	CGCG	1 cut(s)	724
Bsh1285I	CGRYCG	2 cut(s)	351, 901
BshFI	GGCC	3 cut(s)	253, 428, 479
BshTI	ACCGGT	1 cut(s)	346
BshVI	ATCGAT	1 cut(s)	897
BsiEI	CGRYCG	2 cut(s)	351, 901
BsiHKAI	GWGCWC	2 cut(s)	43, 698
BsiSI	CCGG	2 cut(s)	347, 1115
BslFI	GGGAC	1 cut(s)	1097
BslI	CCNNNNNNNGG	3 cut(s)	145, 353, 1120
BsmAI	GTCTC	1 cut(s)	236
BsmFI	GGGAC	1 cut(s)	1097
BsnI	GGCC	3 cut(s)	253, 428, 479
Bso31I	GGTCTC	1 cut(s)	236
Bsp1286I	GDGCHC	2 cut(s)	43, 698
Bsp143I	GATC	4 cut(s)	574, 898, 977, 1030
Bsp19I	CCATGG	1 cut(s)	1134
BspACI	CCGC	4 cut(s)	642, 650, 722, 889
BspANI	GGCC	3 cut(s)	253, 428, 479
BspCNI	CTCAG	8 cut(s)	199, 313, 370, 703, 703, 835, 871, 994
BspDI	ATCGAT	1 cut(s)	897
BspFNI	CGCG	1 cut(s)	724
BspPI	GGATC	2 cut(s)	569, 985
BspTNI	GGTCTC	1 cut(s)	236
BsrDI	GCAATG	2 cut(s)	949, 1135
BsrFI	RCCGGY	1 cut(s)	346
BsrI	ACTGG	1 cut(s)	463
BssAI	RCCGGY	1 cut(s)	346
BssECI	CCNNGG	2 cut(s)	1051, 1134
BssMI	GATC	4 cut(s)	574, 898, 977, 1030
BssSI	CACGAG	1 cut(s)	42
BssT1I	CCWWGG	2 cut(s)	1051, 1134
Bst2BI	CACGAG	1 cut(s)	42
Bst2UI	CCWGG	1 cut(s)	627
Bst4CI	ACNGT	1 cut(s)	797
Bst6I	CTCTTC	1 cut(s)	1165
BstBAI	YACGTR	1 cut(s)	812
BstC8I	GCNNGC	2 cut(s)	539, 820
BstDSI	CCRYGG	1 cut(s)	1134
BstF5I	GGATG	1 cut(s)	1184
BstFNI	CGCG	1 cut(s)	724
BstH2I	RGCGCY	2 cut(s)	8, 263
BstHHI	GCGC	2 cut(s)	7, 262
BstKTI	GATC	4 cut(s)	577, 901, 980, 1033
BstMAI	GTCTC	1 cut(s)	236
BstMBI	GATC	4 cut(s)	574, 898, 977, 1030
BstMCI	CGRYCG	2 cut(s)	351, 901
BstMWI	GCNNNNNNNGC	4 cut(s)	47, 259, 603, 779
BstNI	CCWGG	1 cut(s)	627
BstNSI	RCATGY	3 cut(s)	790, 909, 1015
BstSCI	CCNGG	2 cut(s)	625, 1113
BstSLI	GKGCMC	1 cut(s)	698
BstUI	CGCG	1 cut(s)	724
BstV1I	GCAGC	4 cut(s)	59, 249, 530, 688
BstX2I	RGATCY	3 cut(s)	574, 977, 1030
BstYI	RGATCY	3 cut(s)	574, 977, 1030
Bsu15I	ATCGAT	1 cut(s)	897
Bsu36I	CCTNAGG	3 cut(s)	378, 711, 843
BsuI	GTATCC	2 cut(s)	304, 877
BsuRI	GGCC	3 cut(s)	253, 428, 479
BsuTUI	ATCGAT	1 cut(s)	897
BtgI	CCRYGG	1 cut(s)	1134
BtsCI	GGATG	1 cut(s)	1184
BtsI	GCAGTG	4 cut(s)	696, 704, 775, 828
BtsIMutI	CAGTG	7 cut(s)	318, 456, 696, 698, 704, 775, 828
Cac8I	GCNNGC	2 cut(s)	539, 820
CaiI	CAGNNNCTG	1 cut(s)	988
CfoI	GCGC	2 cut(s)	7, 262
Cfr10I	RCCGGY	1 cut(s)	346
Cfr13I	GGNCC	2 cut(s)	427, 461
ClaI	ATCGAT	1 cut(s)	897
CseI	GACGC	1 cut(s)	586
Csp6I	GTAC	5 cut(s)	489, 581, 813, 903, 1170
CspAI	ACCGGT	1 cut(s)	346
CviAII	CATG	6 cut(s)	94, 481, 787, 906, 1012, 1135
CviQI	GTAC	5 cut(s)	489, 581, 813, 903, 1170
DpnI	GATC	4 cut(s)	576, 900, 979, 1032
DpnII	GATC	4 cut(s)	574, 898, 977, 1030
EaeI	YGGCCR	1 cut(s)	251
Eam1104I	CTCTTC	1 cut(s)	1165
EarI	CTCTTC	1 cut(s)	1165
Eco130I	CCWWGG	2 cut(s)	1051, 1134
Eco31I	GGTCTC	1 cut(s)	236
Eco47I	GGWCC	1 cut(s)	461
Eco47III	AGCGCT	1 cut(s)	261
Eco57I	CTGAAG	2 cut(s)	875, 881
Eco81I	CCTNAGG	3 cut(s)	378, 711, 843
EcoRII	CCWGG	1 cut(s)	625
EcoT14I	CCWWGG	2 cut(s)	1051, 1134
ErhI	CCWWGG	2 cut(s)	1051, 1134
FaeI	CATG	6 cut(s)	97, 484, 790, 909, 1015, 1138
FaqI	GGGAC	1 cut(s)	1097
FatI	CATG	6 cut(s)	93, 480, 786, 905, 1011, 1134
FauI	CCCGC	2 cut(s)	643, 896
Fnu4HI	GCNGC	5 cut(s)	48, 263, 519, 702, 722
FokI	GGATG	1 cut(s)	1171
Fsp4HI	GCNGC	5 cut(s)	48, 263, 519, 702, 722
FspBI	CTAG	2 cut(s)	936, 956
GlaI	GCGC	2 cut(s)	6, 261
GluI	GCNGC	5 cut(s)	48, 263, 519, 702, 722
HaeII	RGCGCY	2 cut(s)	8, 263
HaeIII	GGCC	3 cut(s)	253, 428, 479
HapII	CCGG	2 cut(s)	347, 1115
HgaI	GACGC	1 cut(s)	586
HhaI	GCGC	2 cut(s)	7, 262
Hin1II	CATG	6 cut(s)	97, 484, 790, 909, 1015, 1138
Hin6I	GCGC	2 cut(s)	5, 260
HinP1I	GCGC	2 cut(s)	5, 260
HinfI	GANTC	5 cut(s)	82, 272, 383, 668, 1193
HpaII	CCGG	2 cut(s)	347, 1115
HphI	GGTGA	1 cut(s)	336
Hpy166II	GTNNAC	1 cut(s)	696
Hpy188I	TCNGA	5 cut(s)	81, 208, 288, 602, 1198
Hpy188III	TCNNGA	2 cut(s)	370, 1064
Hpy8I	GTNNAC	1 cut(s)	696
HpyCH4III	ACNGT	1 cut(s)	797
HpyCH4IV	ACGT	1 cut(s)	811
HpyCH4V	TGCA	9 cut(s)	47, 304, 696, 704, 768, 924, 1077, 1087, 1128
HpyF10VI	GCNNNNNNNGC	4 cut(s)	47, 259, 603, 779
HpySE526I	ACGT	1 cut(s)	811
Hsp92II	CATG	6 cut(s)	97, 484, 790, 909, 1015, 1138
HspAI	GCGC	2 cut(s)	5, 260
Kzo9I	GATC	4 cut(s)	574, 898, 977, 1030
LmnI	GCTCC	1 cut(s)	410
Lsp1109I	GCAGC	4 cut(s)	59, 249, 530, 688
LweI	GCATC	1 cut(s)	958
MaeI	CTAG	2 cut(s)	936, 956
MaeII	ACGT	1 cut(s)	811
MaeIII	GTNAC	3 cut(s)	331, 342, 958
MalI	GATC	4 cut(s)	576, 900, 979, 1032
MboI	GATC	4 cut(s)	574, 898, 977, 1030
MboII	GAAGA	2 cut(s)	1152, 1173
MflI	RGATCY	3 cut(s)	574, 977, 1030
MhlI	GDGCHC	2 cut(s)	43, 698
MlsI	TGGCCA	1 cut(s)	253
MluCI	AATT	9 cut(s)	162, 239, 247, 387, 405, 468, 619, 928, 1149
MluNI	TGGCCA	1 cut(s)	253
MlyI	GAGTC	1 cut(s)	76
MmeI	TCCRAC	2 cut(s)	82, 830
Mox20I	TGGCCA	1 cut(s)	253
MroXI	GAANNNNTTC	1 cut(s)	387
MscI	TGGCCA	1 cut(s)	253
Msp20I	TGGCCA	1 cut(s)	253
MspI	CCGG	2 cut(s)	347, 1115
MspR9I	CCNGG	2 cut(s)	627, 1115
MvaI	CCWGG	1 cut(s)	627
MvnI	CGCG	1 cut(s)	724
MwoI	GCNNNNNNNGC	4 cut(s)	47, 259, 603, 779
NciI	CCSGG	1 cut(s)	1115
NcoI	CCATGG	1 cut(s)	1134
NdeII	GATC	4 cut(s)	574, 898, 977, 1030
NlaIII	CATG	6 cut(s)	97, 484, 790, 909, 1015, 1138
NmuCI	GTSAC	1 cut(s)	342
NspI	RCATGY	3 cut(s)	790, 909, 1015
PciI	ACATGT	2 cut(s)	786, 905
PcsI	WCGNNNNNNNCGW	1 cut(s)	493
PdmI	GAANNNNTTC	1 cut(s)	387
PfeI	GAWTC	4 cut(s)	272, 383, 668, 1193
PfoI	TCCNGGA	1 cut(s)	1113
PinAI	ACCGGT	1 cut(s)	346
PkrI	GCNGC	5 cut(s)	49, 264, 520, 703, 723
Ple19I	CGATCG	1 cut(s)	901
PleI	GAGTC	1 cut(s)	76
PpsI	GAGTC	1 cut(s)	76
Ppu21I	YACGTR	1 cut(s)	812
PscI	ACATGT	2 cut(s)	786, 905
PsiI	TTATAA	1 cut(s)	422
Psp6I	CCWGG	1 cut(s)	625
PspGI	CCWGG	1 cut(s)	625
PspPI	GGNCC	2 cut(s)	427, 461
PstNI	CAGNNNCTG	1 cut(s)	988
PsuI	RGATCY	3 cut(s)	574, 977, 1030
PvuI	CGATCG	1 cut(s)	901
RsaI	GTAC	5 cut(s)	490, 582, 814, 904, 1171
RsaNI	GTAC	5 cut(s)	489, 581, 813, 903, 1170
SatI	GCNGC	5 cut(s)	48, 263, 519, 702, 722
Sau3AI	GATC	4 cut(s)	574, 898, 977, 1030
Sau96I	GGNCC	2 cut(s)	427, 461
SchI	GAGTC	1 cut(s)	76
ScrFI	CCNGG	2 cut(s)	627, 1115
SduI	GDGCHC	2 cut(s)	43, 698
SfaNI	GCATC	1 cut(s)	958
SinI	GGWCC	1 cut(s)	461
SmlI	CTYRAG	1 cut(s)	143
SmoI	CTYRAG	1 cut(s)	143
Sse9I	AATT	9 cut(s)	162, 239, 247, 387, 405, 468, 619, 928, 1149
SsiI	CCGC	4 cut(s)	642, 650, 722, 889
SspMI	CTAG	2 cut(s)	936, 956
StyD4I	CCNGG	2 cut(s)	625, 1113
StyI	CCWWGG	2 cut(s)	1051, 1134
TaaI	ACNGT	1 cut(s)	797
TaiI	ACGT	1 cut(s)	814
TaqI	TCGA	3 cut(s)	15, 351, 897
TaqII	GACCGA	1 cut(s)	547
TasI	AATT	9 cut(s)	162, 239, 247, 387, 405, 468, 619, 928, 1149
TatI	WGTACW	2 cut(s)	580, 1169
TauI	GCSGC	1 cut(s)	724
TfiI	GAWTC	4 cut(s)	272, 383, 668, 1193
TscAI	CASTG	7 cut(s)	325, 463, 698, 703, 711, 775, 835
TseFI	GTSAC	1 cut(s)	342
TseI	GCWGC	4 cut(s)	47, 262, 518, 701
Tsp45I	GTSAC	1 cut(s)	342
TspDTI	ATGAA	5 cut(s)	82, 260, 660, 938, 1173
TspGWI	ACGGA	1 cut(s)	631
TspRI	CASTG	7 cut(s)	325, 463, 698, 703, 711, 775, 835
VneI	GTGCAC	1 cut(s)	694
VpaK11BI	GGWCC	1 cut(s)	461
XapI	RAATTY	2 cut(s)	239, 619
XceI	RCATGY	3 cut(s)	790, 909, 1015
XcmI	CCANNNNNNNNNTGG	2 cut(s)	167, 404
XmnI	GAANNNNTTC	1 cut(s)	387
XspI	CTAG	2 cut(s)	936, 956

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.