Rorug03G0251600

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
Physical Location & Seq
Forward (+)
23735308 .. 23735523
216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0251600.1

Sequence Viewer

Length: 216 bp
ATGGCACAGGTTAGCATTCCCAGGGCCTTGGCCTTCTTCTTCATGCAGGTGCTGGTCTTAGTCCTCGCGGCCGCAACTGTCTCGGCTCAGGACAGCGCTCCGGCACCGGCTCCCATGGATGCTGGAGCGGCTTACTCTTTGTCGGTCAATGGTGCGGTTATTGGAGCCTCGATTGCGGTCTCATTCTTCTCCTTGTTCAAGCACTACTTGAATTAG

Protein Analysis

71

Amino Acids

7.3

Weight (kDa)

6.5

Isoelectric Point (pI)

23.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 37
Acc36I ACCTGC 1 cut(s) 37
AccB1I GGYRCC 1 cut(s) 103
AccBSI CCGCTC 1 cut(s) 128
AccII CGCG 1 cut(s) 68
AciI CCGC 5 cut(s) 68, 72, 128, 155, 176
AcoI YGGCCR 1 cut(s) 69
AfeI AGCGCT 1 cut(s) 97
AgsI TTSAA 2 cut(s) 199, 211
AjnI CCWGG 1 cut(s) 20
Alw26I GTCTC 2 cut(s) 85, 184
AlwNI CAGNNNCTG 1 cut(s) 52
Aor51HI AGCGCT 1 cut(s) 97
AoxI GGCC 3 cut(s) 24, 30, 69
AspLEI GCGC 1 cut(s) 98
AspS9I GGNCC 1 cut(s) 24
BanI GGYRCC 1 cut(s) 103
BcgI CGANNNNNNTGC 2 cut(s) 63, 97
BciT130I CCWGG 1 cut(s) 22
BcoDI GTCTC 2 cut(s) 85, 184
BfoI RGCGCY 1 cut(s) 99
BfuAI ACCTGC 1 cut(s) 37
BisI GCNGC 3 cut(s) 69, 72, 129
BlsI GCNGC 3 cut(s) 70, 73, 130
Bme1390I CCNGG 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 24
BmiI GGNNCC 3 cut(s) 105, 111, 166
BmrFI CCNGG 1 cut(s) 22
BmsI GCATC 1 cut(s) 109
BpmI CTGGAG 1 cut(s) 144
Bpu10I CCTNAGC 1 cut(s) 87
BsaI GGTCTC 1 cut(s) 184
BsaJI CCNNGG 4 cut(s) 20, 21, 27, 114
Bse118I RCCGGY 1 cut(s) 106
BseBI CCWGG 1 cut(s) 22
BseDI CCNNGG 4 cut(s) 20, 21, 27, 114
BseGI GGATG 1 cut(s) 124
BseMII CTCAG 1 cut(s) 101
BseX3I CGGCCG 1 cut(s) 69
Bsh1236I CGCG 1 cut(s) 68
Bsh1285I CGRYCG 1 cut(s) 72
BshFI GGCC 3 cut(s) 26, 32, 71
BshNI GGYRCC 1 cut(s) 103
BsiEI CGRYCG 1 cut(s) 72
BsiSI CCGG 2 cut(s) 101, 107
BsmAI GTCTC 2 cut(s) 85, 184
BsmI GAATGC 1 cut(s) 15
BsnI GGCC 3 cut(s) 26, 32, 71
Bso31I GGTCTC 1 cut(s) 184
Bsp19I CCATGG 1 cut(s) 114
BspACI CCGC 5 cut(s) 68, 72, 128, 155, 176
BspANI GGCC 3 cut(s) 26, 32, 71
BspCNI CTCAG 1 cut(s) 100
BspFNI CGCG 1 cut(s) 68
BspLI GGNNCC 3 cut(s) 105, 111, 166
BspMI ACCTGC 1 cut(s) 37
BspT107I GGYRCC 1 cut(s) 103
BspTNI GGTCTC 1 cut(s) 184
BsrBI CCGCTC 1 cut(s) 128
BsrFI RCCGGY 1 cut(s) 106
BssAI RCCGGY 1 cut(s) 106
BssECI CCNNGG 4 cut(s) 20, 21, 27, 114
BssT1I CCWWGG 2 cut(s) 27, 114
Bst2UI CCWGG 1 cut(s) 22
Bst4CI ACNGT 1 cut(s) 79
BstDEI CTNAG 2 cut(s) 58, 87
BstDSI CCRYGG 1 cut(s) 114
BstF5I GGATG 1 cut(s) 124
BstFNI CGCG 1 cut(s) 68
BstH2I RGCGCY 1 cut(s) 99
BstHHI GCGC 1 cut(s) 98
BstMAI GTCTC 2 cut(s) 85, 184
BstMCI CGRYCG 1 cut(s) 72
BstMWI GCNNNNNNNGC 2 cut(s) 128, 173
BstNI CCWGG 1 cut(s) 22
BstSCI CCNGG 1 cut(s) 20
BstUI CGCG 1 cut(s) 68
BstXI CCANNNNNNTGG 1 cut(s) 28
BstZI CGGCCG 1 cut(s) 69
BsuRI GGCC 3 cut(s) 26, 32, 71
BtgI CCRYGG 1 cut(s) 114
BtsCI GGATG 1 cut(s) 124
BveI ACCTGC 1 cut(s) 37
CaiI CAGNNNCTG 1 cut(s) 52
CciNI GCGGCCGC 1 cut(s) 69
CfoI GCGC 1 cut(s) 98
Cfr10I RCCGGY 1 cut(s) 106
Cfr13I GGNCC 1 cut(s) 24
CviAII CATG 2 cut(s) 43, 115
CviJI RGCY 7 cut(s) 26, 32, 71, 86, 110, 131, 167
CviKI_1 RGCY 7 cut(s) 26, 32, 71, 86, 110, 131, 167
DdeI CTNAG 2 cut(s) 58, 87
EaeI YGGCCR 1 cut(s) 69
EagI CGGCCG 1 cut(s) 69
EclXI CGGCCG 1 cut(s) 69
Eco130I CCWWGG 2 cut(s) 27, 114
Eco31I GGTCTC 1 cut(s) 184
Eco47III AGCGCT 1 cut(s) 97
Eco52I CGGCCG 1 cut(s) 69
EcoO109I RGGNCCY 1 cut(s) 24
EcoRII CCWGG 1 cut(s) 20
EcoT14I CCWWGG 2 cut(s) 27, 114
ErhI CCWWGG 2 cut(s) 27, 114
FaeI CATG 2 cut(s) 46, 118
FaiI YATR 2 cut(s) 44, 116
FalI AAGNNNNNCTT 1 cut(s) 191
FatI CATG 2 cut(s) 42, 114
Fnu4HI GCNGC 3 cut(s) 69, 72, 129
FokI GGATG 1 cut(s) 131
Fsp4HI GCNGC 3 cut(s) 69, 72, 129
GlaI GCGC 1 cut(s) 97
GluI GCNGC 3 cut(s) 69, 72, 129
GsuI CTGGAG 1 cut(s) 144
HaeII RGCGCY 1 cut(s) 99
HaeIII GGCC 3 cut(s) 26, 32, 71
HapII CCGG 2 cut(s) 101, 107
HhaI GCGC 1 cut(s) 98
Hin1II CATG 2 cut(s) 46, 118
Hin6I GCGC 1 cut(s) 96
HinP1I GCGC 1 cut(s) 96
HpaII CCGG 2 cut(s) 101, 107
Hpy188III TCNNGA 1 cut(s) 89
HpyAV CCTTC 1 cut(s) 43
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4V TGCA 1 cut(s) 46
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 173
HpyF3I CTNAG 2 cut(s) 58, 87
Hsp92II CATG 2 cut(s) 46, 118
HspAI GCGC 1 cut(s) 96
LmnI GCTCC 4 cut(s) 103, 115, 125, 164
LpnPI CCDG 8 cut(s) 7, 32, 34, 38, 74, 108, 114, 120
LweI GCATC 1 cut(s) 109
MbiI CCGCTC 1 cut(s) 128
MboII GAAGA 3 cut(s) 28, 31, 178
MluCI AATT 1 cut(s) 211
MnlI CCTC 2 cut(s) 74, 178
MslI CAYNNNNRTG 1 cut(s) 47
MspI CCGG 2 cut(s) 101, 107
MspR9I CCNGG 1 cut(s) 22
Mva1269I GAATGC 1 cut(s) 15
MvaI CCWGG 1 cut(s) 22
MvnI CGCG 1 cut(s) 68
MwoI GCNNNNNNNGC 2 cut(s) 128, 173
NcoI CCATGG 1 cut(s) 114
NlaIII CATG 2 cut(s) 46, 118
NlaIV GGNNCC 3 cut(s) 105, 111, 166
NmeAIII GCCGAG 1 cut(s) 62
NotI GCGGCCGC 1 cut(s) 69
PaqCI CACCTGC 1 cut(s) 37
PasI CCCWGGG 1 cut(s) 21
PctI GAATGC 1 cut(s) 15
PkrI GCNGC 3 cut(s) 70, 73, 130
Psp6I CCWGG 1 cut(s) 20
PspGI CCWGG 1 cut(s) 20
PspN4I GGNNCC 3 cut(s) 105, 111, 166
PspPI GGNCC 1 cut(s) 24
PstNI CAGNNNCTG 1 cut(s) 52
RseI CAYNNNNRTG 1 cut(s) 47
SatI GCNGC 3 cut(s) 69, 72, 129
Sau96I GGNCC 1 cut(s) 24
ScrFI CCNGG 1 cut(s) 22
SetI ASST 2 cut(s) 12, 51
SfaNI GCATC 1 cut(s) 109
SmiMI CAYNNNNRTG 1 cut(s) 47
Sse9I AATT 1 cut(s) 211
SsiI CCGC 5 cut(s) 68, 72, 128, 155, 176
StyD4I CCNGG 1 cut(s) 20
StyI CCWWGG 2 cut(s) 27, 114
TaaI ACNGT 1 cut(s) 79
TaqI TCGA 1 cut(s) 170
TaqII GACCGA 1 cut(s) 133
TasI AATT 1 cut(s) 211
TauI GCSGC 3 cut(s) 71, 74, 131
TspDTI ATGAA 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.