Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_5g36560 FvH4_5g36560 FvH4_5g36560 FvH4_5g36560 FvH4_5g36560 FvH4_5g36560 |
| malus_domestica | MD08G1188600.v1.1 MD15G1376400.v1.1 |
| prunus_persica | Prupe.1G523100_v2.0.a1 Prupe.1G523100_v2.0.a1 |
| pyrus_communis | pycom08g16230 pycom15g33630 |
| rosa_chinensis | RchiOBHm_Chr3g0492071 RchiOBHm_Chr7g0242241 |
| rosa_laevigata | RLG00000000904 RLG00000022795 |
| rosa_multiflora | Rmu_sc0003846.1_g000008 Rmu_sc0031707.1_g000001 |
| rosa_roxburghii | Rroxscaffold_6G00392560 |
| rosa_rugosa | Rorug03G0251600 Rorug03G0251700 Rorug07G0314300 |
| rosa_samantha | Rh3AG301800 Rh3BG337600 Rh3BG337700 Rh3CG335200 Rh3DG337300 Rh7AG469700 Rh7BG439500 Rh7CG486300 Rh7DG454700 |
| rosa_wichuraiana | Rw3G026640 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 353 |
| AccI | GTMKAC | 1 cut(s) | 504 |
| AclWI | GGATC | 4 cut(s) | 569, 621, 893, 985 |
| AcoI | YGGCCR | 1 cut(s) | 251 |
| AcsI | RAATTY | 3 cut(s) | 239, 619, 1079 |
| AcuI | CTGAAG | 3 cut(s) | 342, 399, 732 |
| AcyI | GRCGYC | 1 cut(s) | 83 |
| AfaI | GTAC | 6 cut(s) | 561, 582, 814, 829, 840, 1074 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 123, 353, 1120 |
| AflIII | ACRYGT | 4 cut(s) | 786, 809, 905, 1011 |
| AgsI | TTSAA | 4 cut(s) | 146, 392, 518, 1018 |
| AjnI | CCWGG | 1 cut(s) | 954 |
| AluBI | AGCT | 9 cut(s) | 50, 142, 211, 311, 521, 833, 941, 985, 1021 |
| AluI | AGCT | 9 cut(s) | 50, 142, 211, 311, 521, 833, 941, 985, 1021 |
| Alw21I | GWGCWC | 4 cut(s) | 43, 698, 775, 855 |
| Alw26I | GTCTC | 2 cut(s) | 236, 523 |
| Alw44I | GTGCAC | 1 cut(s) | 694 |
| AlwI | GGATC | 4 cut(s) | 569, 621, 893, 985 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 988 |
| Ama87I | CYCGRG | 2 cut(s) | 42, 842 |
| AoxI | GGCC | 3 cut(s) | 251, 426, 477 |
| ApaLI | GTGCAC | 1 cut(s) | 694 |
| ApeKI | GCWGC | 2 cut(s) | 47, 1167 |
| ApoI | RAATTY | 3 cut(s) | 239, 619, 1079 |
| AspS9I | GGNCC | 2 cut(s) | 427, 461 |
| AsuC2I | CCSGG | 1 cut(s) | 627 |
| AsuHPI | GGTGA | 4 cut(s) | 336, 668, 1118, 1176 |
| AvaI | CYCGRG | 2 cut(s) | 42, 842 |
| AvaII | GGWCC | 1 cut(s) | 461 |
| BaeGI | GKGCMC | 1 cut(s) | 698 |
| BalI | TGGCCA | 1 cut(s) | 253 |
| Bbv12I | GWGCWC | 4 cut(s) | 43, 698, 775, 855 |
| BbvI | GCAGC | 2 cut(s) | 59, 1154 |
| BccI | CCATC | 5 cut(s) | 283, 347, 461, 492, 868 |
| BciT130I | CCWGG | 1 cut(s) | 956 |
| BcnI | CCSGG | 1 cut(s) | 627 |
| BcoDI | GTCTC | 2 cut(s) | 236, 523 |
| BfaI | CTAG | 3 cut(s) | 51, 776, 936 |
| BisI | GCNGC | 2 cut(s) | 48, 1168 |
| BlsI | GCNGC | 2 cut(s) | 49, 1169 |
| Bme1390I | CCNGG | 2 cut(s) | 627, 956 |
| Bme18I | GGWCC | 1 cut(s) | 461 |
| BmeT110I | CYCGRG | 2 cut(s) | 42, 842 |
| BmgT120I | GGNCC | 2 cut(s) | 427, 461 |
| BmiI | GGNNCC | 1 cut(s) | 115 |
| BmrFI | CCNGG | 2 cut(s) | 627, 956 |
| BmrI | ACTGGG | 1 cut(s) | 179 |
| BmsI | GCATC | 1 cut(s) | 958 |
| BmuI | ACTGGG | 1 cut(s) | 179 |
| BpuMI | CCSGG | 1 cut(s) | 627 |
| Bsa29I | ATCGAT | 1 cut(s) | 1152 |
| BsaAI | YACGTR | 2 cut(s) | 812, 1076 |
| BsaHI | GRCGYC | 1 cut(s) | 83 |
| BsaI | GGTCTC | 2 cut(s) | 236, 523 |
| BsaJI | CCNNGG | 2 cut(s) | 843, 1134 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 45, 75 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 123, 353, 1120 |
| Bse1I | ACTGG | 5 cut(s) | 174, 254, 347, 463, 756 |
| Bse3DI | GCAATG | 4 cut(s) | 260, 704, 949, 1135 |
| BseBI | CCWGG | 1 cut(s) | 956 |
| BseCI | ATCGAT | 1 cut(s) | 1152 |
| BseDI | CCNNGG | 2 cut(s) | 843, 1134 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 123, 353, 1120 |
| BseMI | GCAATG | 4 cut(s) | 260, 704, 949, 1135 |
| BseMII | CTCAG | 2 cut(s) | 198, 995 |
| BseNI | ACTGG | 5 cut(s) | 174, 254, 347, 463, 756 |
| BseRI | GAGGAG | 1 cut(s) | 488 |
| BseSI | GKGCMC | 1 cut(s) | 698 |
| BseXI | GCAGC | 2 cut(s) | 59, 1154 |
| BsgI | GTGCAG | 2 cut(s) | 1019, 1153 |
| BshFI | GGCC | 3 cut(s) | 253, 428, 479 |
| BshVI | ATCGAT | 1 cut(s) | 1152 |
| BsiHKAI | GWGCWC | 4 cut(s) | 43, 698, 775, 855 |
| BsiHKCI | CYCGRG | 2 cut(s) | 42, 842 |
| BsiSI | CCGG | 1 cut(s) | 627 |
| BslFI | GGGAC | 2 cut(s) | 876, 1097 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 123, 353, 1120 |
| BsmAI | GTCTC | 2 cut(s) | 236, 523 |
| BsmFI | GGGAC | 2 cut(s) | 876, 1097 |
| BsnI | GGCC | 3 cut(s) | 253, 428, 479 |
| Bso31I | GGTCTC | 2 cut(s) | 236, 523 |
| BsoBI | CYCGRG | 2 cut(s) | 42, 842 |
| Bsp1286I | GDGCHC | 4 cut(s) | 43, 698, 775, 855 |
| Bsp1407I | TGTACA | 1 cut(s) | 559 |
| Bsp143I | GATC | 4 cut(s) | 574, 613, 898, 977 |
| Bsp19I | CCATGG | 1 cut(s) | 1134 |
| BspANI | GGCC | 3 cut(s) | 253, 428, 479 |
| BspCNI | CTCAG | 2 cut(s) | 199, 994 |
| BspDI | ATCGAT | 1 cut(s) | 1152 |
| BspLI | GGNNCC | 1 cut(s) | 115 |
| BspPI | GGATC | 4 cut(s) | 569, 621, 893, 985 |
| BspTNI | GGTCTC | 2 cut(s) | 236, 523 |
| BsrDI | GCAATG | 4 cut(s) | 260, 704, 949, 1135 |
| BsrGI | TGTACA | 1 cut(s) | 559 |
| BsrI | ACTGG | 5 cut(s) | 174, 254, 347, 463, 756 |
| BssECI | CCNNGG | 2 cut(s) | 843, 1134 |
| BssMI | GATC | 4 cut(s) | 574, 613, 898, 977 |
| BssNI | GRCGYC | 1 cut(s) | 83 |
| BssT1I | CCWWGG | 1 cut(s) | 1134 |
| Bst2UI | CCWGG | 1 cut(s) | 956 |
| Bst4CI | ACNGT | 3 cut(s) | 38, 693, 797 |
| Bst6I | CTCTTC | 5 cut(s) | 141, 318, 375, 708, 1165 |
| BstACI | GRCGYC | 1 cut(s) | 83 |
| BstAUI | TGTACA | 1 cut(s) | 559 |
| BstBAI | YACGTR | 2 cut(s) | 812, 1076 |
| BstC8I | GCNNGC | 1 cut(s) | 820 |
| BstDEI | CTNAG | 5 cut(s) | 207, 571, 584, 981, 1022 |
| BstDSI | CCRYGG | 1 cut(s) | 1134 |
| BstKTI | GATC | 4 cut(s) | 577, 616, 901, 980 |
| BstMAI | GTCTC | 2 cut(s) | 236, 523 |
| BstMBI | GATC | 4 cut(s) | 574, 613, 898, 977 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 47, 779 |
| BstNI | CCWGG | 1 cut(s) | 956 |
| BstNSI | RCATGY | 3 cut(s) | 790, 909, 1015 |
| BstSCI | CCNGG | 2 cut(s) | 625, 954 |
| BstSLI | GKGCMC | 1 cut(s) | 698 |
| BstV1I | GCAGC | 2 cut(s) | 59, 1154 |
| BstX2I | RGATCY | 2 cut(s) | 574, 977 |
| BstYI | RGATCY | 2 cut(s) | 574, 977 |
| Bsu15I | ATCGAT | 1 cut(s) | 1152 |
| BsuRI | GGCC | 3 cut(s) | 253, 428, 479 |
| BsuTUI | ATCGAT | 1 cut(s) | 1152 |
| BtgI | CCRYGG | 1 cut(s) | 1134 |
| BtgZI | GCGATG | 1 cut(s) | 887 |
| BtsI | GCAGTG | 2 cut(s) | 696, 775 |
| BtsIMutI | CAGTG | 6 cut(s) | 318, 456, 696, 698, 749, 775 |
| Cac8I | GCNNGC | 1 cut(s) | 820 |
| CaiI | CAGNNNCTG | 1 cut(s) | 988 |
| Cfr13I | GGNCC | 2 cut(s) | 427, 461 |
| ClaI | ATCGAT | 1 cut(s) | 1152 |
| CseI | GACGC | 1 cut(s) | 91 |
| Csp6I | GTAC | 6 cut(s) | 560, 581, 813, 828, 839, 1073 |
| CviAII | CATG | 6 cut(s) | 94, 787, 906, 1012, 1093, 1135 |
| CviQI | GTAC | 6 cut(s) | 560, 581, 813, 828, 839, 1073 |
| DdeI | CTNAG | 5 cut(s) | 207, 571, 584, 981, 1022 |
| DpnI | GATC | 4 cut(s) | 576, 615, 900, 979 |
| DpnII | GATC | 4 cut(s) | 574, 613, 898, 977 |
| EaeI | YGGCCR | 1 cut(s) | 251 |
| Eam1104I | CTCTTC | 5 cut(s) | 141, 318, 375, 708, 1165 |
| EarI | CTCTTC | 5 cut(s) | 141, 318, 375, 708, 1165 |
| Eco130I | CCWWGG | 1 cut(s) | 1134 |
| Eco31I | GGTCTC | 2 cut(s) | 236, 523 |
| Eco32I | GATATC | 1 cut(s) | 1156 |
| Eco47I | GGWCC | 1 cut(s) | 461 |
| Eco57I | CTGAAG | 3 cut(s) | 342, 399, 732 |
| Eco88I | CYCGRG | 2 cut(s) | 42, 842 |
| EcoRII | CCWGG | 1 cut(s) | 954 |
| EcoRV | GATATC | 1 cut(s) | 1156 |
| EcoT14I | CCWWGG | 1 cut(s) | 1134 |
| ErhI | CCWWGG | 1 cut(s) | 1134 |
| FaeI | CATG | 6 cut(s) | 97, 790, 909, 1015, 1096, 1138 |
| FaqI | GGGAC | 2 cut(s) | 876, 1097 |
| FatI | CATG | 6 cut(s) | 93, 786, 905, 1011, 1092, 1134 |
| FblI | GTMKAC | 1 cut(s) | 504 |
| Fnu4HI | GCNGC | 2 cut(s) | 48, 1168 |
| Fsp4HI | GCNGC | 2 cut(s) | 48, 1168 |
| FspBI | CTAG | 3 cut(s) | 51, 776, 936 |
| GluI | GCNGC | 2 cut(s) | 48, 1168 |
| HaeIII | GGCC | 3 cut(s) | 253, 428, 479 |
| HapII | CCGG | 1 cut(s) | 627 |
| HgaI | GACGC | 1 cut(s) | 91 |
| Hin1I | GRCGYC | 1 cut(s) | 83 |
| Hin1II | CATG | 6 cut(s) | 97, 790, 909, 1015, 1096, 1138 |
| HincII | GTYRAC | 1 cut(s) | 129 |
| HindII | GTYRAC | 1 cut(s) | 129 |
| HinfI | GANTC | 4 cut(s) | 272, 383, 668, 1149 |
| HpaII | CCGG | 1 cut(s) | 627 |
| HphI | GGTGA | 4 cut(s) | 336, 668, 1118, 1176 |
| Hpy166II | GTNNAC | 3 cut(s) | 129, 505, 696 |
| Hpy188I | TCNGA | 4 cut(s) | 208, 288, 618, 1101 |
| Hpy188III | TCNNGA | 2 cut(s) | 370, 1064 |
| Hpy8I | GTNNAC | 3 cut(s) | 129, 505, 696 |
| HpyAV | CCTTC | 1 cut(s) | 1050 |
| HpyCH4III | ACNGT | 3 cut(s) | 38, 693, 797 |
| HpyCH4IV | ACGT | 2 cut(s) | 811, 1075 |
| HpyCH4V | TGCA | 9 cut(s) | 304, 689, 696, 768, 924, 1036, 1087, 1128, 1170 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 47, 779 |
| HpyF3I | CTNAG | 5 cut(s) | 207, 571, 584, 981, 1022 |
| HpySE526I | ACGT | 2 cut(s) | 811, 1075 |
| Hsp92I | GRCGYC | 1 cut(s) | 83 |
| Hsp92II | CATG | 6 cut(s) | 97, 790, 909, 1015, 1096, 1138 |
| Kzo9I | GATC | 4 cut(s) | 574, 613, 898, 977 |
| LmnI | GCTCC | 1 cut(s) | 410 |
| Lsp1109I | GCAGC | 2 cut(s) | 59, 1154 |
| LweI | GCATC | 1 cut(s) | 958 |
| MaeI | CTAG | 3 cut(s) | 51, 776, 936 |
| MaeII | ACGT | 2 cut(s) | 811, 1075 |
| MaeIII | GTNAC | 3 cut(s) | 331, 342, 958 |
| MalI | GATC | 4 cut(s) | 576, 615, 900, 979 |
| MboI | GATC | 4 cut(s) | 574, 613, 898, 977 |
| MboII | GAAGA | 5 cut(s) | 158, 335, 392, 725, 1152 |
| MflI | RGATCY | 2 cut(s) | 574, 977 |
| MhlI | GDGCHC | 4 cut(s) | 43, 698, 775, 855 |
| MlsI | TGGCCA | 1 cut(s) | 253 |
| MluCI | AATT | 8 cut(s) | 239, 387, 405, 468, 619, 928, 1000, 1079 |
| MluNI | TGGCCA | 1 cut(s) | 253 |
| MlyI | GAGTC | 1 cut(s) | 392 |
| Mox20I | TGGCCA | 1 cut(s) | 253 |
| MscI | TGGCCA | 1 cut(s) | 253 |
| MseI | TTAA | 1 cut(s) | 33 |
| MslI | CAYNNNNRTG | 1 cut(s) | 98 |
| Msp20I | TGGCCA | 1 cut(s) | 253 |
| MspI | CCGG | 1 cut(s) | 627 |
| MspR9I | CCNGG | 2 cut(s) | 627, 956 |
| MvaI | CCWGG | 1 cut(s) | 956 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 47, 779 |
| NciI | CCSGG | 1 cut(s) | 627 |
| NcoI | CCATGG | 1 cut(s) | 1134 |
| NdeII | GATC | 4 cut(s) | 574, 613, 898, 977 |
| NlaIII | CATG | 6 cut(s) | 97, 790, 909, 1015, 1096, 1138 |
| NlaIV | GGNNCC | 1 cut(s) | 115 |
| NmuCI | GTSAC | 1 cut(s) | 342 |
| NspI | RCATGY | 3 cut(s) | 790, 909, 1015 |
| PaeR7I | CTCGAG | 1 cut(s) | 42 |
| PciI | ACATGT | 3 cut(s) | 786, 905, 1011 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 493 |
| PfeI | GAWTC | 3 cut(s) | 272, 668, 1149 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 353 |
| PkrI | GCNGC | 2 cut(s) | 49, 1169 |
| PleI | GAGTC | 1 cut(s) | 391 |
| PpsI | GAGTC | 1 cut(s) | 391 |
| Ppu21I | YACGTR | 2 cut(s) | 812, 1076 |
| PscI | ACATGT | 3 cut(s) | 786, 905, 1011 |
| Psp6I | CCWGG | 1 cut(s) | 954 |
| PspGI | CCWGG | 1 cut(s) | 954 |
| PspN4I | GGNNCC | 1 cut(s) | 115 |
| PspPI | GGNCC | 2 cut(s) | 427, 461 |
| PspXI | VCTCGAGB | 1 cut(s) | 42 |
| PstNI | CAGNNNCTG | 1 cut(s) | 988 |
| PsuI | RGATCY | 2 cut(s) | 574, 977 |
| RsaI | GTAC | 6 cut(s) | 561, 582, 814, 829, 840, 1074 |
| RsaNI | GTAC | 6 cut(s) | 560, 581, 813, 828, 839, 1073 |
| RseI | CAYNNNNRTG | 1 cut(s) | 98 |
| SaqAI | TTAA | 1 cut(s) | 33 |
| SatI | GCNGC | 2 cut(s) | 48, 1168 |
| Sau3AI | GATC | 4 cut(s) | 574, 613, 898, 977 |
| Sau96I | GGNCC | 2 cut(s) | 427, 461 |
| SchI | GAGTC | 1 cut(s) | 392 |
| ScrFI | CCNGG | 2 cut(s) | 627, 956 |
| SduI | GDGCHC | 4 cut(s) | 43, 698, 775, 855 |
| SfaNI | GCATC | 1 cut(s) | 958 |
| Sfr274I | CTCGAG | 1 cut(s) | 42 |
| SinI | GGWCC | 1 cut(s) | 461 |
| SlaI | CTCGAG | 1 cut(s) | 42 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 98 |
| SmlI | CTYRAG | 1 cut(s) | 42 |
| SmoI | CTYRAG | 1 cut(s) | 42 |
| Sse9I | AATT | 8 cut(s) | 239, 387, 405, 468, 619, 928, 1000, 1079 |
| SspMI | CTAG | 3 cut(s) | 51, 776, 936 |
| StyD4I | CCNGG | 2 cut(s) | 625, 954 |
| StyI | CCWWGG | 1 cut(s) | 1134 |
| TaaI | ACNGT | 3 cut(s) | 38, 693, 797 |
| TaiI | ACGT | 2 cut(s) | 814, 1078 |
| TaqI | TCGA | 3 cut(s) | 43, 72, 1152 |
| TasI | AATT | 8 cut(s) | 239, 387, 405, 468, 619, 928, 1000, 1079 |
| TatI | WGTACW | 2 cut(s) | 559, 580 |
| TfiI | GAWTC | 3 cut(s) | 272, 668, 1149 |
| Tru1I | TTAA | 1 cut(s) | 33 |
| Tru9I | TTAA | 1 cut(s) | 33 |
| TscAI | CASTG | 6 cut(s) | 325, 463, 698, 703, 756, 775 |
| TseFI | GTSAC | 1 cut(s) | 342 |
| TseI | GCWGC | 2 cut(s) | 47, 1167 |
| Tsp45I | GTSAC | 1 cut(s) | 342 |
| TspDTI | ATGAA | 7 cut(s) | 82, 260, 285, 660, 693, 938, 1170 |
| TspRI | CASTG | 6 cut(s) | 325, 463, 698, 703, 756, 775 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 353 |
| VneI | GTGCAC | 1 cut(s) | 694 |
| VpaK11BI | GGWCC | 1 cut(s) | 461 |
| XapI | RAATTY | 3 cut(s) | 239, 619, 1079 |
| XceI | RCATGY | 3 cut(s) | 790, 909, 1015 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 404 |
| XhoI | CTCGAG | 1 cut(s) | 42 |
| XmiI | GTMKAC | 1 cut(s) | 504 |
| XspI | CTAG | 3 cut(s) | 51, 776, 936 |