RLG00000000904

Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0004542
Physical Location & Seq
Reverse (-)
7773658 .. 7780308
6651 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000904

Sequence Viewer

Length: 1155 bp
ATGGCGCTTCGTTTCGAGGTGTTGGGGAGGTTCAATCGCGCTCGTGCAGCTCAACTGACACTCCCTCACTTTGTATGTCAGACTCCTCTTTTCATGCCTGTTGGAACACAAGGAACTATAAAAGGGTTGGCAACTGACCAGCTTGAGGAGATTGGTTGCCAAATAATTCTTGGGAATACATATCATTTGGCACTTCGACCTACCTCTGAGCTTATTGACGAGATGGGAGGCCTCCACAAATTTATGAACTGGCCAAGAGCTCTGCTTACTGATTCTGGTGGCTTTCAGATGGTATCCTTATTGCATTTAGCTGACATCACTGAGAAGGGTGTAATATTTCAGTCACCAGTCGATGGAAAGCCAATGCTCTTGACACCTGAGGAATCAATTCAAATCCAAAATAGAATTGGAGCAGATATTATAATGGCCCTTGATGATGTAGTGAAAGCCACCATCACTGGTCCAAGAATTGAGGAGGCCATACCGGGCGAGCAGAACTTATTTGGTATTGTTCAAGGTGGCCTAGATCCTGTACTAAGGGATATATGCGTCAGAGGCTTAGTAGAACGGAATTTGCCTGGCTATGCCATAGGCGGTCTTGCAGCTGGCGAAAGTAAAGATTCATTTTGGCGCGTTGTTGCTCAGTGCACTGCTGCACTGCCTGAGGACAAGCCGCGATATGTTATGGGCGTTGGTTATCCATTGGACATTGTAGTTTGCAGTGCTTTAGGTGCTGACATGTATGACTCTGTTTATCCAACACGTACTGCTCGCTTTGGCACAGCTCTTATACCTGAGGGAGTGCTGAAGCTGAAGCATAAAGCTATGGCTGAGGATATCCGCCCTATCGATCCTACATGTGCTTGTATGATATGCAAGAATTATACTAGATCTTACATTCATTGCCTAGTTACAAAAGATGCTATGGGATCTCAGCTTCTGTCATTTCACAATCTGTATTACATGCTAAAGCTAAGTAGAGATCTACACTCGTCAATAATCCAAGGAACATTTCCAGAGTTTGTATGCAACTTTCTACAAATATTGTTTCCTAAAGGCGATGTTCCAGAATGGGTCTGTAATGCCATGGAGGTTGCTGGAATTGACATCTCTTCCTGTTGTACTGCATTTTCTTCATCCCAAGATTCAGATTAG

Protein Analysis

385

Amino Acids

42.11

Weight (kDa)

5.33

Isoelectric Point (pI)

41.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 13 - 347 7.2e-133 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 422
AccB7I CCANNNNNTGG 1 cut(s) 353
AccII CGCG 3 cut(s) 39, 633, 676
AciI CCGC 3 cut(s) 594, 674, 841
AclWI GGATC 3 cut(s) 521, 845, 937
AcoI YGGCCR 1 cut(s) 251
AcsI RAATTY 2 cut(s) 239, 571
AcuI CTGAAG 2 cut(s) 827, 833
AfaI GTAC 3 cut(s) 534, 766, 1123
AfiI CCNNNNNNNGG 2 cut(s) 145, 353
AflIII ACRYGT 3 cut(s) 738, 761, 857
AgsI TTSAA 3 cut(s) 34, 392, 515
AjnI CCWGG 1 cut(s) 577
AjuI GAANNNNNNNTTGG 2 cut(s) 996, 1028
Alw21I GWGCWC 2 cut(s) 262, 650
Alw44I GTGCAC 1 cut(s) 646
AlwI GGATC 3 cut(s) 521, 845, 937
AlwNI CAGNNNCTG 1 cut(s) 940
AoxI GGCC 5 cut(s) 229, 251, 426, 477, 520
ApaLI GTGCAC 1 cut(s) 646
ApeKI GCWGC 3 cut(s) 47, 602, 653
ApoI RAATTY 2 cut(s) 239, 571
Asp700I GAANNNNTTC 1 cut(s) 387
AspLEI GCGC 3 cut(s) 7, 41, 633
AspS9I GGNCC 2 cut(s) 427, 461
AsuC2I CCSGG 1 cut(s) 486
AsuHPI GGTGA 1 cut(s) 336
AvaII GGWCC 1 cut(s) 461
AxyI CCTNAGG 3 cut(s) 378, 663, 795
BaeGI GKGCMC 1 cut(s) 650
BalI TGGCCA 1 cut(s) 253
BanII GRGCYC 1 cut(s) 262
BauI CACGAG 1 cut(s) 42
Bbv12I GWGCWC 2 cut(s) 262, 650
BbvCI CCTCAGC 1 cut(s) 831
BbvI GCAGC 3 cut(s) 59, 614, 640
BccI CCATC 4 cut(s) 217, 283, 347, 461
BciT130I CCWGG 1 cut(s) 579
BciVI GTATCC 1 cut(s) 304
BcnI CCSGG 1 cut(s) 486
BfaI CTAG 3 cut(s) 524, 888, 908
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 1 cut(s) 304
BglII AGATCT 2 cut(s) 890, 982
BisI GCNGC 4 cut(s) 48, 603, 654, 674
BlsI GCNGC 4 cut(s) 49, 604, 655, 675
Bme1390I CCNGG 2 cut(s) 486, 579
Bme18I GGWCC 1 cut(s) 461
BmgT120I GGNCC 2 cut(s) 427, 461
BmrFI CCNGG 2 cut(s) 486, 579
BmsI GCATC 1 cut(s) 910
Bpu10I CCTNAGC 1 cut(s) 831
BpuEI CTTGAG 1 cut(s) 164
BpuMI CCSGG 1 cut(s) 486
Bsa29I ATCGAT 1 cut(s) 849
BsaAI YACGTR 1 cut(s) 764
BsaJI CCNNGG 2 cut(s) 1003, 1086
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 353
Bse1I ACTGG 3 cut(s) 254, 347, 463
Bse21I CCTNAGG 3 cut(s) 378, 663, 795
Bse3DI GCAATG 1 cut(s) 901
BseBI CCWGG 1 cut(s) 579
BseCI ATCGAT 1 cut(s) 849
BseDI CCNNGG 2 cut(s) 1003, 1086
BseGI GGATG 1 cut(s) 1136
BseLI CCNNNNNNNGG 2 cut(s) 145, 353
BseMI GCAATG 1 cut(s) 901
BseMII CTCAG 8 cut(s) 198, 312, 369, 654, 656, 786, 822, 947
BseNI ACTGG 3 cut(s) 254, 347, 463
BseRI GAGGAG 3 cut(s) 75, 161, 488
BseSI GKGCMC 1 cut(s) 650
BseXI GCAGC 3 cut(s) 59, 614, 640
BsgI GTGCAG 2 cut(s) 66, 639
Bsh1236I CGCG 3 cut(s) 39, 633, 676
BshFI GGCC 5 cut(s) 231, 253, 428, 479, 522
BshVI ATCGAT 1 cut(s) 849
BsiHKAI GWGCWC 2 cut(s) 262, 650
BsiSI CCGG 1 cut(s) 485
BslI CCNNNNNNNGG 2 cut(s) 145, 353
BsnI GGCC 5 cut(s) 231, 253, 428, 479, 522
Bsp1286I GDGCHC 2 cut(s) 262, 650
Bsp143I GATC 5 cut(s) 526, 850, 890, 929, 982
Bsp19I CCATGG 1 cut(s) 1086
BspACI CCGC 3 cut(s) 594, 674, 841
BspANI GGCC 5 cut(s) 231, 253, 428, 479, 522
BspCNI CTCAG 8 cut(s) 199, 313, 370, 655, 655, 787, 823, 946
BspDI ATCGAT 1 cut(s) 849
BspFNI CGCG 3 cut(s) 39, 633, 676
BspPI GGATC 3 cut(s) 521, 845, 937
BsrDI GCAATG 1 cut(s) 901
BsrI ACTGG 3 cut(s) 254, 347, 463
BssECI CCNNGG 2 cut(s) 1003, 1086
BssMI GATC 5 cut(s) 526, 850, 890, 929, 982
BssSI CACGAG 1 cut(s) 42
BssT1I CCWWGG 2 cut(s) 1003, 1086
Bst2BI CACGAG 1 cut(s) 42
Bst2UI CCWGG 1 cut(s) 579
Bst6I CTCTTC 1 cut(s) 1117
BstBAI YACGTR 1 cut(s) 764
BstC8I GCNNGC 3 cut(s) 491, 607, 772
BstDSI CCRYGG 1 cut(s) 1086
BstF5I GGATG 1 cut(s) 1136
BstFNI CGCG 3 cut(s) 39, 633, 676
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 41, 633
BstKTI GATC 5 cut(s) 529, 853, 893, 932, 985
BstMBI GATC 5 cut(s) 526, 850, 890, 929, 982
BstMWI GCNNNNNNNGC 3 cut(s) 47, 555, 731
BstNI CCWGG 1 cut(s) 579
BstNSI RCATGY 3 cut(s) 742, 861, 967
BstSCI CCNGG 2 cut(s) 484, 577
BstSLI GKGCMC 1 cut(s) 650
BstUI CGCG 3 cut(s) 39, 633, 676
BstV1I GCAGC 3 cut(s) 59, 614, 640
BstX2I RGATCY 4 cut(s) 526, 890, 929, 982
BstYI RGATCY 4 cut(s) 526, 890, 929, 982
Bsu15I ATCGAT 1 cut(s) 849
Bsu36I CCTNAGG 3 cut(s) 378, 663, 795
BsuI GTATCC 1 cut(s) 304
BsuRI GGCC 5 cut(s) 231, 253, 428, 479, 522
BsuTUI ATCGAT 1 cut(s) 849
BtgI CCRYGG 1 cut(s) 1086
BtgZI GCGATG 1 cut(s) 1074
BtsCI GGATG 1 cut(s) 1136
BtsI GCAGTG 3 cut(s) 648, 656, 727
BtsIMutI CAGTG 6 cut(s) 318, 456, 648, 650, 656, 727
Cac8I GCNNGC 3 cut(s) 491, 607, 772
CaiI CAGNNNCTG 1 cut(s) 940
CfoI GCGC 3 cut(s) 7, 41, 633
Cfr13I GGNCC 2 cut(s) 427, 461
ClaI ATCGAT 1 cut(s) 849
CseI GACGC 1 cut(s) 538
Csp6I GTAC 3 cut(s) 533, 765, 1122
CviAII CATG 5 cut(s) 94, 739, 858, 964, 1087
CviQI GTAC 3 cut(s) 533, 765, 1122
DpnI GATC 5 cut(s) 528, 852, 892, 931, 984
DpnII GATC 5 cut(s) 526, 850, 890, 929, 982
EaeI YGGCCR 1 cut(s) 251
Eam1104I CTCTTC 1 cut(s) 1117
EarI CTCTTC 1 cut(s) 1117
EciI GGCGGA 1 cut(s) 830
Ecl136II GAGCTC 1 cut(s) 260
Eco130I CCWWGG 2 cut(s) 1003, 1086
Eco147I AGGCCT 1 cut(s) 231
Eco24I GRGCYC 1 cut(s) 262
Eco32I GATATC 1 cut(s) 838
Eco47I GGWCC 1 cut(s) 461
Eco53kI GAGCTC 1 cut(s) 260
Eco57I CTGAAG 2 cut(s) 827, 833
Eco81I CCTNAGG 3 cut(s) 378, 663, 795
EcoICRI GAGCTC 1 cut(s) 260
EcoRII CCWGG 1 cut(s) 577
EcoRV GATATC 1 cut(s) 838
EcoT14I CCWWGG 2 cut(s) 1003, 1086
EcoT38I GRGCYC 1 cut(s) 262
ErhI CCWWGG 2 cut(s) 1003, 1086
FaeI CATG 5 cut(s) 97, 742, 861, 967, 1090
FatI CATG 5 cut(s) 93, 738, 857, 963, 1086
Fnu4HI GCNGC 4 cut(s) 48, 603, 654, 674
FokI GGATG 1 cut(s) 1123
FriOI GRGCYC 1 cut(s) 262
Fsp4HI GCNGC 4 cut(s) 48, 603, 654, 674
FspBI CTAG 3 cut(s) 524, 888, 908
GlaI GCGC 3 cut(s) 6, 40, 632
GluI GCNGC 4 cut(s) 48, 603, 654, 674
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 5 cut(s) 231, 253, 428, 479, 522
HapII CCGG 1 cut(s) 485
HgaI GACGC 1 cut(s) 538
HhaI GCGC 3 cut(s) 7, 41, 633
Hin1II CATG 5 cut(s) 97, 742, 861, 967, 1090
Hin6I GCGC 3 cut(s) 5, 39, 631
HinP1I GCGC 3 cut(s) 5, 39, 631
HinfI GANTC 6 cut(s) 82, 272, 383, 620, 746, 1145
HpaII CCGG 1 cut(s) 485
HphI GGTGA 1 cut(s) 336
Hpy166II GTNNAC 1 cut(s) 648
Hpy188I TCNGA 5 cut(s) 81, 208, 288, 554, 1150
Hpy188III TCNNGA 3 cut(s) 370, 1016, 1067
Hpy8I GTNNAC 1 cut(s) 648
HpyAV CCTTC 1 cut(s) 319
HpyCH4IV ACGT 1 cut(s) 763
HpyCH4V TGCA 9 cut(s) 47, 304, 602, 648, 656, 720, 876, 1029, 1127
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 555, 731
HpySE526I ACGT 1 cut(s) 763
Hsp92II CATG 5 cut(s) 97, 742, 861, 967, 1090
HspAI GCGC 3 cut(s) 5, 39, 631
Kzo9I GATC 5 cut(s) 526, 850, 890, 929, 982
LmnI GCTCC 1 cut(s) 410
Lsp1109I GCAGC 3 cut(s) 59, 614, 640
LweI GCATC 1 cut(s) 910
MaeI CTAG 3 cut(s) 524, 888, 908
MaeII ACGT 1 cut(s) 763
MaeIII GTNAC 2 cut(s) 342, 910
MalI GATC 5 cut(s) 528, 852, 892, 931, 984
MboI GATC 5 cut(s) 526, 850, 890, 929, 982
MboII GAAGA 2 cut(s) 1104, 1125
MflI RGATCY 4 cut(s) 526, 890, 929, 982
MhlI GDGCHC 2 cut(s) 262, 650
MlsI TGGCCA 1 cut(s) 253
MluCI AATT 8 cut(s) 165, 239, 387, 405, 468, 571, 880, 1101
MluNI TGGCCA 1 cut(s) 253
MlyI GAGTC 2 cut(s) 76, 740
MmeI TCCRAC 2 cut(s) 82, 782
Mox20I TGGCCA 1 cut(s) 253
MroXI GAANNNNTTC 1 cut(s) 387
MscI TGGCCA 1 cut(s) 253
Msp20I TGGCCA 1 cut(s) 253
MspA1I CMGCKG 1 cut(s) 605
MspI CCGG 1 cut(s) 485
MspR9I CCNGG 2 cut(s) 486, 579
MvaI CCWGG 1 cut(s) 579
MvnI CGCG 3 cut(s) 39, 633, 676
MwoI GCNNNNNNNGC 3 cut(s) 47, 555, 731
NciI CCSGG 1 cut(s) 486
NcoI CCATGG 1 cut(s) 1086
NdeII GATC 5 cut(s) 526, 850, 890, 929, 982
NlaIII CATG 5 cut(s) 97, 742, 861, 967, 1090
NmuCI GTSAC 1 cut(s) 342
NspI RCATGY 3 cut(s) 742, 861, 967
PceI AGGCCT 1 cut(s) 231
PciI ACATGT 2 cut(s) 738, 857
PdmI GAANNNNTTC 1 cut(s) 387
PfeI GAWTC 4 cut(s) 272, 383, 620, 1145
PflMI CCANNNNNTGG 1 cut(s) 353
PkrI GCNGC 4 cut(s) 49, 604, 655, 675
PleI GAGTC 2 cut(s) 76, 740
PpsI GAGTC 2 cut(s) 76, 740
Ppu21I YACGTR 1 cut(s) 764
PscI ACATGT 2 cut(s) 738, 857
PsiI TTATAA 1 cut(s) 422
Psp124BI GAGCTC 1 cut(s) 262
Psp6I CCWGG 1 cut(s) 577
PspGI CCWGG 1 cut(s) 577
PspPI GGNCC 2 cut(s) 427, 461
PstNI CAGNNNCTG 1 cut(s) 940
PsuI RGATCY 4 cut(s) 526, 890, 929, 982
PvuII CAGCTG 1 cut(s) 605
RsaI GTAC 3 cut(s) 534, 766, 1123
RsaNI GTAC 3 cut(s) 533, 765, 1122
SacI GAGCTC 1 cut(s) 262
SatI GCNGC 4 cut(s) 48, 603, 654, 674
Sau3AI GATC 5 cut(s) 526, 850, 890, 929, 982
Sau96I GGNCC 2 cut(s) 427, 461
SchI GAGTC 2 cut(s) 76, 740
ScrFI CCNGG 2 cut(s) 486, 579
SduI GDGCHC 2 cut(s) 262, 650
SfaNI GCATC 1 cut(s) 910
SinI GGWCC 1 cut(s) 461
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
Sse9I AATT 8 cut(s) 165, 239, 387, 405, 468, 571, 880, 1101
SseBI AGGCCT 1 cut(s) 231
SsiI CCGC 3 cut(s) 594, 674, 841
SspI AATATT 2 cut(s) 336, 1044
SspMI CTAG 3 cut(s) 524, 888, 908
SstI GAGCTC 1 cut(s) 262
StuI AGGCCT 1 cut(s) 231
StyD4I CCNGG 2 cut(s) 484, 577
StyI CCWWGG 2 cut(s) 1003, 1086
TaiI ACGT 1 cut(s) 766
TaqI TCGA 4 cut(s) 15, 196, 351, 849
TasI AATT 8 cut(s) 165, 239, 387, 405, 468, 571, 880, 1101
TatI WGTACW 2 cut(s) 532, 1121
TauI GCSGC 1 cut(s) 676
TfiI GAWTC 4 cut(s) 272, 383, 620, 1145
TscAI CASTG 6 cut(s) 325, 463, 650, 655, 663, 727
TseFI GTSAC 1 cut(s) 342
TseI GCWGC 3 cut(s) 47, 602, 653
Tsp45I GTSAC 1 cut(s) 342
TspDTI ATGAA 5 cut(s) 82, 260, 612, 890, 1125
TspGWI ACGGA 1 cut(s) 583
TspRI CASTG 6 cut(s) 325, 463, 650, 655, 663, 727
Van91I CCANNNNNTGG 1 cut(s) 353
VneI GTGCAC 1 cut(s) 646
VpaK11BI GGWCC 1 cut(s) 461
XapI RAATTY 2 cut(s) 239, 571
XceI RCATGY 3 cut(s) 742, 861, 967
XcmI CCANNNNNNNNNTGG 2 cut(s) 167, 404
XmnI GAANNNNTTC 1 cut(s) 387
XspI CTAG 3 cut(s) 524, 888, 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.