Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_5g36560 FvH4_5g36560 FvH4_5g36560 FvH4_5g36560 FvH4_5g36560 FvH4_5g36560 |
| malus_domestica | MD08G1188600.v1.1 MD15G1376400.v1.1 |
| prunus_persica | Prupe.1G523100_v2.0.a1 Prupe.1G523100_v2.0.a1 |
| pyrus_communis | pycom08g16230 pycom15g33630 |
| rosa_chinensis | RchiOBHm_Chr3g0492071 RchiOBHm_Chr7g0242241 |
| rosa_laevigata | RLG00000000904 RLG00000022795 |
| rosa_multiflora | Rmu_sc0003846.1_g000008 Rmu_sc0031707.1_g000001 |
| rosa_roxburghii | Rroxscaffold_6G00392560 |
| rosa_rugosa | Rorug03G0251600 Rorug03G0251700 Rorug07G0314300 |
| rosa_samantha | Rh3AG301800 Rh3BG337600 Rh3BG337700 Rh3CG335200 Rh3DG337300 Rh7AG469700 Rh7BG439500 Rh7CG486300 Rh7DG454700 |
| rosa_wichuraiana | Rw3G026640 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 392 |
| AciI | CCGC | 4 cut(s) | 125, 495, 507, 602 |
| AclWI | GGATC | 3 cut(s) | 464, 746, 838 |
| AcoI | YGGCCR | 1 cut(s) | 290 |
| AcsI | RAATTY | 2 cut(s) | 278, 932 |
| AcuI | CTGAAG | 1 cut(s) | 753 |
| AdeI | CACNNNGTG | 1 cut(s) | 112 |
| AfaI | GTAC | 4 cut(s) | 456, 477, 667, 682 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 392 |
| AflIII | ACRYGT | 3 cut(s) | 639, 662, 758 |
| AgsI | TTSAA | 3 cut(s) | 185, 431, 897 |
| AjnI | CCWGG | 1 cut(s) | 807 |
| Alw21I | GWGCWC | 4 cut(s) | 82, 199, 551, 628 |
| Alw26I | GTCTC | 1 cut(s) | 275 |
| Alw44I | GTGCAC | 1 cut(s) | 547 |
| AlwI | GGATC | 3 cut(s) | 464, 746, 838 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 841 |
| Ama87I | CYCGRG | 2 cut(s) | 695, 968 |
| AoxI | GGCC | 1 cut(s) | 290 |
| ApaLI | GTGCAC | 1 cut(s) | 547 |
| ApeKI | GCWGC | 3 cut(s) | 86, 554, 1020 |
| ApoI | RAATTY | 2 cut(s) | 278, 932 |
| AsuHPI | GGTGA | 4 cut(s) | 349, 375, 521, 971 |
| AsuII | TTCGAA | 1 cut(s) | 35 |
| AvaI | CYCGRG | 2 cut(s) | 695, 968 |
| AxyI | CCTNAGG | 2 cut(s) | 417, 564 |
| BaeGI | GKGCMC | 2 cut(s) | 551, 969 |
| BalI | TGGCCA | 1 cut(s) | 292 |
| BanII | GRGCYC | 1 cut(s) | 199 |
| BauI | CACGAG | 1 cut(s) | 81 |
| Bbv12I | GWGCWC | 4 cut(s) | 82, 199, 551, 628 |
| BbvI | GCAGC | 3 cut(s) | 98, 541, 1007 |
| BccI | CCATC | 3 cut(s) | 322, 386, 721 |
| BciT130I | CCWGG | 1 cut(s) | 809 |
| BcoDI | GTCTC | 1 cut(s) | 275 |
| BfaI | CTAG | 3 cut(s) | 90, 629, 789 |
| BisI | GCNGC | 3 cut(s) | 87, 555, 1021 |
| BlsI | GCNGC | 3 cut(s) | 88, 556, 1022 |
| Bme1390I | CCNGG | 1 cut(s) | 809 |
| BmeT110I | CYCGRG | 2 cut(s) | 695, 968 |
| BmrFI | CCNGG | 1 cut(s) | 809 |
| BmsI | GCATC | 2 cut(s) | 811, 973 |
| Bpu14I | TTCGAA | 1 cut(s) | 35 |
| Bsa29I | ATCGAT | 3 cut(s) | 9, 41, 1005 |
| BsaAI | YACGTR | 2 cut(s) | 219, 665 |
| BsaI | GGTCTC | 1 cut(s) | 275 |
| BsaJI | CCNNGG | 2 cut(s) | 696, 987 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 392 |
| Bse1I | ACTGG | 2 cut(s) | 293, 386 |
| Bse21I | CCTNAGG | 2 cut(s) | 417, 564 |
| Bse3DI | GCAATG | 3 cut(s) | 299, 557, 802 |
| BseBI | CCWGG | 1 cut(s) | 809 |
| BseCI | ATCGAT | 3 cut(s) | 9, 41, 1005 |
| BseDI | CCNNGG | 2 cut(s) | 696, 987 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 392 |
| BseMI | GCAATG | 3 cut(s) | 299, 557, 802 |
| BseMII | CTCAG | 4 cut(s) | 408, 555, 848, 866 |
| BseNI | ACTGG | 2 cut(s) | 293, 386 |
| BseRI | GAGGAG | 2 cut(s) | 434, 581 |
| BseSI | GKGCMC | 2 cut(s) | 551, 969 |
| BseXI | GCAGC | 3 cut(s) | 98, 541, 1007 |
| BsgI | GTGCAG | 3 cut(s) | 105, 872, 1006 |
| BshFI | GGCC | 1 cut(s) | 292 |
| BshVI | ATCGAT | 3 cut(s) | 9, 41, 1005 |
| BsiHKAI | GWGCWC | 4 cut(s) | 82, 199, 551, 628 |
| BsiHKCI | CYCGRG | 2 cut(s) | 695, 968 |
| BslFI | GGGAC | 1 cut(s) | 1024 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 392 |
| BsmAI | GTCTC | 1 cut(s) | 275 |
| BsmFI | GGGAC | 1 cut(s) | 1024 |
| BsnI | GGCC | 1 cut(s) | 292 |
| Bso31I | GGTCTC | 1 cut(s) | 275 |
| BsoBI | CYCGRG | 2 cut(s) | 695, 968 |
| Bsp119I | TTCGAA | 1 cut(s) | 35 |
| Bsp1286I | GDGCHC | 5 cut(s) | 82, 199, 551, 628, 969 |
| Bsp1407I | TGTACA | 1 cut(s) | 454 |
| Bsp143I | GATC | 3 cut(s) | 469, 751, 830 |
| Bsp19I | CCATGG | 1 cut(s) | 987 |
| BspACI | CCGC | 4 cut(s) | 125, 495, 507, 602 |
| BspANI | GGCC | 1 cut(s) | 292 |
| BspCNI | CTCAG | 4 cut(s) | 409, 556, 847, 867 |
| BspDI | ATCGAT | 3 cut(s) | 9, 41, 1005 |
| BspPI | GGATC | 3 cut(s) | 464, 746, 838 |
| BspT104I | TTCGAA | 1 cut(s) | 35 |
| BspTNI | GGTCTC | 1 cut(s) | 275 |
| BsrDI | GCAATG | 3 cut(s) | 299, 557, 802 |
| BsrGI | TGTACA | 1 cut(s) | 454 |
| BsrI | ACTGG | 2 cut(s) | 293, 386 |
| BssECI | CCNNGG | 2 cut(s) | 696, 987 |
| BssMI | GATC | 3 cut(s) | 469, 751, 830 |
| BssSI | CACGAG | 1 cut(s) | 81 |
| BssT1I | CCWWGG | 1 cut(s) | 987 |
| Bst2BI | CACGAG | 1 cut(s) | 81 |
| Bst2UI | CCWGG | 1 cut(s) | 809 |
| Bst4CI | ACNGT | 3 cut(s) | 77, 546, 650 |
| Bst6I | CTCTTC | 1 cut(s) | 898 |
| BstAUI | TGTACA | 1 cut(s) | 454 |
| BstBAI | YACGTR | 2 cut(s) | 219, 665 |
| BstBI | TTCGAA | 1 cut(s) | 35 |
| BstC8I | GCNNGC | 2 cut(s) | 505, 673 |
| BstDEI | CTNAG | 6 cut(s) | 417, 466, 479, 564, 834, 875 |
| BstDSI | CCRYGG | 1 cut(s) | 987 |
| BstKTI | GATC | 3 cut(s) | 472, 754, 833 |
| BstMAI | GTCTC | 1 cut(s) | 275 |
| BstMBI | GATC | 3 cut(s) | 469, 751, 830 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 86, 133, 632 |
| BstNI | CCWGG | 1 cut(s) | 809 |
| BstNSI | RCATGY | 2 cut(s) | 643, 762 |
| BstSCI | CCNGG | 1 cut(s) | 807 |
| BstSLI | GKGCMC | 2 cut(s) | 551, 969 |
| BstSNI | TACGTA | 1 cut(s) | 219 |
| BstV1I | GCAGC | 3 cut(s) | 98, 541, 1007 |
| BstX2I | RGATCY | 2 cut(s) | 469, 830 |
| BstYI | RGATCY | 2 cut(s) | 469, 830 |
| Bsu15I | ATCGAT | 3 cut(s) | 9, 41, 1005 |
| Bsu36I | CCTNAGG | 2 cut(s) | 417, 564 |
| BsuRI | GGCC | 1 cut(s) | 292 |
| BsuTUI | ATCGAT | 3 cut(s) | 9, 41, 1005 |
| BtgI | CCRYGG | 1 cut(s) | 987 |
| BtgZI | GCGATG | 1 cut(s) | 740 |
| BtsI | GCAGTG | 2 cut(s) | 549, 628 |
| BtsIMutI | CAGTG | 3 cut(s) | 549, 551, 628 |
| Cac8I | GCNNGC | 2 cut(s) | 505, 673 |
| CaiI | CAGNNNCTG | 1 cut(s) | 841 |
| ClaI | ATCGAT | 3 cut(s) | 9, 41, 1005 |
| Csp6I | GTAC | 4 cut(s) | 455, 476, 666, 681 |
| CviAII | CATG | 5 cut(s) | 133, 640, 759, 865, 988 |
| CviQI | GTAC | 4 cut(s) | 455, 476, 666, 681 |
| DdeI | CTNAG | 6 cut(s) | 417, 466, 479, 564, 834, 875 |
| DpnI | GATC | 3 cut(s) | 471, 753, 832 |
| DpnII | GATC | 3 cut(s) | 469, 751, 830 |
| DraIII | CACNNNGTG | 1 cut(s) | 112 |
| EaeI | YGGCCR | 1 cut(s) | 290 |
| Eam1104I | CTCTTC | 1 cut(s) | 898 |
| EarI | CTCTTC | 1 cut(s) | 898 |
| Ecl136II | GAGCTC | 1 cut(s) | 197 |
| Eco105I | TACGTA | 1 cut(s) | 219 |
| Eco130I | CCWWGG | 1 cut(s) | 987 |
| Eco24I | GRGCYC | 1 cut(s) | 199 |
| Eco31I | GGTCTC | 1 cut(s) | 275 |
| Eco53kI | GAGCTC | 1 cut(s) | 197 |
| Eco57I | CTGAAG | 1 cut(s) | 753 |
| Eco81I | CCTNAGG | 2 cut(s) | 417, 564 |
| Eco88I | CYCGRG | 2 cut(s) | 695, 968 |
| EcoICRI | GAGCTC | 1 cut(s) | 197 |
| EcoRII | CCWGG | 1 cut(s) | 807 |
| EcoT14I | CCWWGG | 1 cut(s) | 987 |
| EcoT38I | GRGCYC | 1 cut(s) | 199 |
| ErhI | CCWWGG | 1 cut(s) | 987 |
| FaeI | CATG | 5 cut(s) | 136, 643, 762, 868, 991 |
| FaqI | GGGAC | 1 cut(s) | 1024 |
| FatI | CATG | 5 cut(s) | 132, 639, 758, 864, 987 |
| Fnu4HI | GCNGC | 3 cut(s) | 87, 555, 1021 |
| FriOI | GRGCYC | 1 cut(s) | 199 |
| Fsp4HI | GCNGC | 3 cut(s) | 87, 555, 1021 |
| FspBI | CTAG | 3 cut(s) | 90, 629, 789 |
| GluI | GCNGC | 3 cut(s) | 87, 555, 1021 |
| HaeIII | GGCC | 1 cut(s) | 292 |
| Hin1II | CATG | 5 cut(s) | 136, 643, 762, 868, 991 |
| HincII | GTYRAC | 1 cut(s) | 168 |
| HindII | GTYRAC | 1 cut(s) | 168 |
| HinfI | GANTC | 4 cut(s) | 311, 422, 521, 1002 |
| HphI | GGTGA | 4 cut(s) | 349, 375, 521, 971 |
| Hpy166II | GTNNAC | 2 cut(s) | 168, 549 |
| Hpy188I | TCNGA | 3 cut(s) | 247, 327, 954 |
| Hpy188III | TCNNGA | 3 cut(s) | 254, 409, 917 |
| Hpy8I | GTNNAC | 2 cut(s) | 168, 549 |
| HpyAV | CCTTC | 2 cut(s) | 359, 903 |
| HpyCH4III | ACNGT | 3 cut(s) | 77, 546, 650 |
| HpyCH4IV | ACGT | 2 cut(s) | 218, 664 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 86, 133, 632 |
| HpyF3I | CTNAG | 6 cut(s) | 417, 466, 479, 564, 834, 875 |
| HpySE526I | ACGT | 2 cut(s) | 218, 664 |
| Hsp92II | CATG | 5 cut(s) | 136, 643, 762, 868, 991 |
| Kzo9I | GATC | 3 cut(s) | 469, 751, 830 |
| LmnI | GCTCC | 1 cut(s) | 194 |
| Lsp1109I | GCAGC | 3 cut(s) | 98, 541, 1007 |
| LweI | GCATC | 2 cut(s) | 811, 973 |
| MaeI | CTAG | 3 cut(s) | 90, 629, 789 |
| MaeII | ACGT | 2 cut(s) | 218, 664 |
| MaeIII | GTNAC | 3 cut(s) | 370, 381, 811 |
| MalI | GATC | 3 cut(s) | 471, 753, 832 |
| MboI | GATC | 3 cut(s) | 469, 751, 830 |
| MboII | GAAGA | 5 cut(s) | 197, 374, 746, 885, 1005 |
| MflI | RGATCY | 2 cut(s) | 469, 830 |
| MhlI | GDGCHC | 5 cut(s) | 82, 199, 551, 628, 969 |
| MlsI | TGGCCA | 1 cut(s) | 292 |
| MluCI | AATT | 5 cut(s) | 278, 426, 781, 853, 932 |
| MluNI | TGGCCA | 1 cut(s) | 292 |
| MlyI | GAGTC | 1 cut(s) | 431 |
| MmeI | TCCRAC | 2 cut(s) | 121, 263 |
| MnlI | CCTC | 9 cut(s) | 60, 114, 253, 260, 412, 559, 691, 898, 985 |
| Mox20I | TGGCCA | 1 cut(s) | 292 |
| MscI | TGGCCA | 1 cut(s) | 292 |
| MseI | TTAA | 1 cut(s) | 72 |
| Msp20I | TGGCCA | 1 cut(s) | 292 |
| MspA1I | CMGCKG | 1 cut(s) | 604 |
| MspR9I | CCNGG | 1 cut(s) | 809 |
| MvaI | CCWGG | 1 cut(s) | 809 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 86, 133, 632 |
| NcoI | CCATGG | 1 cut(s) | 987 |
| NdeII | GATC | 3 cut(s) | 469, 751, 830 |
| NlaIII | CATG | 5 cut(s) | 136, 643, 762, 868, 991 |
| NmuCI | GTSAC | 1 cut(s) | 381 |
| NspI | RCATGY | 2 cut(s) | 643, 762 |
| NspV | TTCGAA | 1 cut(s) | 35 |
| PciI | ACATGT | 2 cut(s) | 639, 758 |
| PfeI | GAWTC | 3 cut(s) | 311, 521, 1002 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 392 |
| PkrI | GCNGC | 3 cut(s) | 88, 556, 1022 |
| PleI | GAGTC | 1 cut(s) | 430 |
| PpsI | GAGTC | 1 cut(s) | 430 |
| Ppu21I | YACGTR | 2 cut(s) | 219, 665 |
| PscI | ACATGT | 2 cut(s) | 639, 758 |
| Psp124BI | GAGCTC | 1 cut(s) | 199 |
| Psp6I | CCWGG | 1 cut(s) | 807 |
| PspGI | CCWGG | 1 cut(s) | 807 |
| PstNI | CAGNNNCTG | 1 cut(s) | 841 |
| PsuI | RGATCY | 2 cut(s) | 469, 830 |
| RsaI | GTAC | 4 cut(s) | 456, 477, 667, 682 |
| RsaNI | GTAC | 4 cut(s) | 455, 476, 666, 681 |
| SacI | GAGCTC | 1 cut(s) | 199 |
| SaqAI | TTAA | 1 cut(s) | 72 |
| SatI | GCNGC | 3 cut(s) | 87, 555, 1021 |
| Sau3AI | GATC | 3 cut(s) | 469, 751, 830 |
| SchI | GAGTC | 1 cut(s) | 431 |
| ScrFI | CCNGG | 1 cut(s) | 809 |
| SduI | GDGCHC | 5 cut(s) | 82, 199, 551, 628, 969 |
| SfaNI | GCATC | 2 cut(s) | 811, 973 |
| SfuI | TTCGAA | 1 cut(s) | 35 |
| SnaBI | TACGTA | 1 cut(s) | 219 |
| Sse9I | AATT | 5 cut(s) | 278, 426, 781, 853, 932 |
| SsiI | CCGC | 4 cut(s) | 125, 495, 507, 602 |
| SspMI | CTAG | 3 cut(s) | 90, 629, 789 |
| SstI | GAGCTC | 1 cut(s) | 199 |
| StyD4I | CCNGG | 1 cut(s) | 807 |
| StyI | CCWWGG | 1 cut(s) | 987 |
| TaaI | ACNGT | 3 cut(s) | 77, 546, 650 |
| TaiI | ACGT | 2 cut(s) | 221, 667 |
| TaqI | TCGA | 5 cut(s) | 9, 35, 41, 903, 1005 |
| TasI | AATT | 5 cut(s) | 278, 426, 781, 853, 932 |
| TatI | WGTACW | 2 cut(s) | 454, 475 |
| TfiI | GAWTC | 3 cut(s) | 311, 521, 1002 |
| Tru1I | TTAA | 1 cut(s) | 72 |
| Tru9I | TTAA | 1 cut(s) | 72 |
| TscAI | CASTG | 3 cut(s) | 551, 556, 628 |
| TseFI | GTSAC | 1 cut(s) | 381 |
| TseI | GCWGC | 3 cut(s) | 86, 554, 1020 |
| Tsp45I | GTSAC | 1 cut(s) | 381 |
| TspDTI | ATGAA | 8 cut(s) | 17, 20, 121, 299, 513, 791, 884, 1023 |
| TspRI | CASTG | 3 cut(s) | 551, 556, 628 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 392 |
| VneI | GTGCAC | 1 cut(s) | 547 |
| XapI | RAATTY | 2 cut(s) | 278, 932 |
| XceI | RCATGY | 2 cut(s) | 643, 762 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 443 |
| XspI | CTAG | 3 cut(s) | 90, 629, 789 |