RchiOBHm_Chr6g0257801

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0020571
Physical Location & Seq
Forward (+)
13180792 .. 13181404
613 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 462 bp
ATGGTAAAGATTGGTCTACGTACAGTTATGAATATGATTAAGGCAAACCAGGTTTGCTGGTTTTCACTTATAGACCGGAAAAGGAGGCTGCTGATCGAAGGAATAGCTTATCAGTGGAACTATATGGTGGAAAACCAATGCAAGTCTACCATTCCTTTTTCTTTTTCTTTTTTGTTACCAAGGTCTAAGTGTACTACATTTATCGATGGGGACAATGGGATGAAAGCTGGGACTTGCGGTAACCGTGGTGAATCGTCTCTGATGATGAACAAAACGAAGTCAATCATTTTAATGAACTACTTTTCTAGTAACCCTAATATCATTCGATCTTGCAATGATAATCCAGATCCGTTGCTAGACATGATCAAAACCTGCCAGGTAGCCACGGCTAATCAATGGCCTAATTTCGTTGCCGCTGACTTTTACACCGAAAATGCAACATATATCAATGCGACAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

17.41

Weight (kDa)

8.96

Isoelectric Point (pI)

37.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC_cat PF26178 69 - 145 8.9e-26 PI-PLC-like catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020571)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0257801
rosa_laevigata RLG00000014728
rosa_roxburghii Rroxscaffold_7G00208980
rosa_samantha Rh6AG084700 Rh6CG074100 Rh6DG071000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 380
AccI GTMKAC 2 cut(s) 16, 146
AciI CCGC 2 cut(s) 237, 414
AclWI GGATC 1 cut(s) 341
AfaI GTAC 2 cut(s) 22, 193
AjnI CCWGG 2 cut(s) 48, 375
AluBI AGCT 2 cut(s) 107, 227
AluI AGCT 2 cut(s) 107, 227
Alw26I GTCTC 1 cut(s) 261
AlwI GGATC 1 cut(s) 341
AoxI GGCC 1 cut(s) 398
ApeKI GCWGC 1 cut(s) 88
AsuHPI GGTGA 1 cut(s) 260
BbvI GCAGC 1 cut(s) 75
BccI CCATC 1 cut(s) 200
BceAI ACGGC 1 cut(s) 402
BciT130I CCWGG 2 cut(s) 50, 377
BclI TGATCA 1 cut(s) 363
BcoDI GTCTC 1 cut(s) 261
BfaI CTAG 2 cut(s) 306, 356
BfuAI ACCTGC 1 cut(s) 380
BisI GCNGC 2 cut(s) 89, 414
BlsI GCNGC 2 cut(s) 90, 415
Bme1390I CCNGG 2 cut(s) 50, 377
BmrFI CCNGG 2 cut(s) 50, 377
Bsa29I ATCGAT 1 cut(s) 204
BsaAI YACGTR 1 cut(s) 20
BsaJI CCNNGG 3 cut(s) 179, 244, 384
BsaWI WCCGGW 1 cut(s) 75
Bse3DI GCAATG 1 cut(s) 340
BseBI CCWGG 2 cut(s) 50, 377
BseCI ATCGAT 1 cut(s) 204
BseDI CCNNGG 3 cut(s) 179, 244, 384
BseGI GGATG 1 cut(s) 225
BseMI GCAATG 1 cut(s) 340
BseXI GCAGC 1 cut(s) 75
BseYI CCCAGC 1 cut(s) 227
BshFI GGCC 1 cut(s) 400
BshVI ATCGAT 1 cut(s) 204
BsiSI CCGG 1 cut(s) 76
BslFI GGGAC 2 cut(s) 224, 244
BsmAI GTCTC 1 cut(s) 261
BsmBI CGTCTC 1 cut(s) 261
BsmFI GGGAC 2 cut(s) 224, 244
BsnI GGCC 1 cut(s) 400
Bsp143I GATC 4 cut(s) 93, 326, 346, 363
BspACI CCGC 2 cut(s) 237, 414
BspANI GGCC 1 cut(s) 400
BspDI ATCGAT 1 cut(s) 204
BspMI ACCTGC 1 cut(s) 380
BspPI GGATC 1 cut(s) 341
BsrDI GCAATG 1 cut(s) 340
BssECI CCNNGG 3 cut(s) 179, 244, 384
BssMI GATC 4 cut(s) 93, 326, 346, 363
BssT1I CCWWGG 1 cut(s) 179
Bst2UI CCWGG 2 cut(s) 50, 377
Bst4CI ACNGT 2 cut(s) 25, 245
BstBAI YACGTR 1 cut(s) 20
BstDEI CTNAG 1 cut(s) 186
BstDSI CCRYGG 2 cut(s) 244, 384
BstEII GGTNACC 1 cut(s) 239
BstF5I GGATG 1 cut(s) 225
BstKTI GATC 4 cut(s) 96, 329, 349, 366
BstMAI GTCTC 1 cut(s) 261
BstMBI GATC 4 cut(s) 93, 326, 346, 363
BstNI CCWGG 2 cut(s) 50, 377
BstPI GGTNACC 1 cut(s) 239
BstSCI CCNGG 2 cut(s) 48, 375
BstSNI TACGTA 1 cut(s) 20
BstV1I GCAGC 1 cut(s) 75
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
Bsu15I ATCGAT 1 cut(s) 204
BsuRI GGCC 1 cut(s) 400
BsuTUI ATCGAT 1 cut(s) 204
BtgI CCRYGG 2 cut(s) 244, 384
BtsCI GGATG 1 cut(s) 225
BtsIMutI CAGTG 1 cut(s) 119
BveI ACCTGC 1 cut(s) 380
ClaI ATCGAT 1 cut(s) 204
CsiI ACCWGGT 1 cut(s) 48
Csp6I GTAC 2 cut(s) 21, 192
CviAII CATG 1 cut(s) 361
CviJI RGCY 6 cut(s) 88, 107, 227, 383, 389, 400
CviKI_1 RGCY 6 cut(s) 88, 107, 227, 383, 389, 400
CviQI GTAC 2 cut(s) 21, 192
DdeI CTNAG 1 cut(s) 186
DpnI GATC 4 cut(s) 95, 328, 348, 365
DpnII GATC 4 cut(s) 93, 326, 346, 363
Eco105I TACGTA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 179
Eco91I GGTNACC 1 cut(s) 239
EcoO65I GGTNACC 1 cut(s) 239
EcoRII CCWGG 2 cut(s) 48, 375
EcoT14I CCWWGG 1 cut(s) 179
ErhI CCWWGG 1 cut(s) 179
Esp3I CGTCTC 1 cut(s) 261
FaeI CATG 1 cut(s) 364
FaiI YATR 8 cut(s) 29, 35, 71, 123, 125, 362, 442, 444
FaqI GGGAC 2 cut(s) 224, 244
FatI CATG 1 cut(s) 360
FbaI TGATCA 1 cut(s) 363
FblI GTMKAC 2 cut(s) 16, 146
Fnu4HI GCNGC 2 cut(s) 89, 414
FokI GGATG 1 cut(s) 232
Fsp4HI GCNGC 2 cut(s) 89, 414
FspBI CTAG 2 cut(s) 306, 356
GluI GCNGC 2 cut(s) 89, 414
GsaI CCCAGC 1 cut(s) 231
HaeIII GGCC 1 cut(s) 400
HapII CCGG 1 cut(s) 76
Hin1II CATG 1 cut(s) 364
HinfI GANTC 1 cut(s) 251
HpaII CCGG 1 cut(s) 76
HphI GGTGA 1 cut(s) 260
Hpy166II GTNNAC 3 cut(s) 17, 147, 192
Hpy188I TCNGA 1 cut(s) 261
Hpy188III TCNNGA 1 cut(s) 344
Hpy8I GTNNAC 3 cut(s) 17, 147, 192
HpyAV CCTTC 1 cut(s) 92
HpyCH4III ACNGT 2 cut(s) 25, 245
HpyCH4IV ACGT 1 cut(s) 19
HpyCH4V TGCA 3 cut(s) 141, 333, 437
HpyF3I CTNAG 1 cut(s) 186
HpySE526I ACGT 1 cut(s) 19
Hsp92II CATG 1 cut(s) 364
Ksp22I TGATCA 1 cut(s) 363
Kzo9I GATC 4 cut(s) 93, 326, 346, 363
LpnPI CCDG 9 cut(s) 35, 43, 62, 89, 213, 357, 362, 385, 389
Lsp1109I GCAGC 1 cut(s) 75
MabI ACCWGGT 1 cut(s) 48
MaeI CTAG 2 cut(s) 306, 356
MaeII ACGT 1 cut(s) 19
MaeIII GTNAC 3 cut(s) 174, 239, 308
MalI GATC 4 cut(s) 95, 328, 348, 365
MboI GATC 4 cut(s) 93, 326, 346, 363
MflI RGATCY 1 cut(s) 346
MluCI AATT 2 cut(s) 403, 456
MnlI CCTC 1 cut(s) 78
MseI TTAA 3 cut(s) 39, 290, 460
MslI CAYNNNNRTG 1 cut(s) 290
MspA1I CMGCKG 1 cut(s) 416
MspI CCGG 1 cut(s) 76
MspR9I CCNGG 2 cut(s) 50, 377
MvaI CCWGG 2 cut(s) 50, 377
NdeII GATC 4 cut(s) 93, 326, 346, 363
NlaIII CATG 1 cut(s) 364
PfeI GAWTC 1 cut(s) 251
PkrI GCNGC 2 cut(s) 90, 415
Ppu21I YACGTR 1 cut(s) 20
Psp6I CCWGG 2 cut(s) 48, 375
PspEI GGTNACC 1 cut(s) 239
PspFI CCCAGC 1 cut(s) 227
PspGI CCWGG 2 cut(s) 48, 375
PsuI RGATCY 1 cut(s) 346
RsaI GTAC 2 cut(s) 22, 193
RsaNI GTAC 2 cut(s) 21, 192
RseI CAYNNNNRTG 1 cut(s) 290
SaqAI TTAA 3 cut(s) 39, 290, 460
SatI GCNGC 2 cut(s) 89, 414
Sau3AI GATC 4 cut(s) 93, 326, 346, 363
ScrFI CCNGG 2 cut(s) 50, 377
SetI ASST 7 cut(s) 22, 54, 109, 185, 229, 374, 381
SexAI ACCWGGT 1 cut(s) 48
SmiMI CAYNNNNRTG 1 cut(s) 290
SnaBI TACGTA 1 cut(s) 20
Sse9I AATT 2 cut(s) 403, 456
SsiI CCGC 2 cut(s) 237, 414
SspMI CTAG 2 cut(s) 306, 356
StyD4I CCNGG 2 cut(s) 48, 375
StyI CCWWGG 1 cut(s) 179
TaaI ACNGT 2 cut(s) 25, 245
TaiI ACGT 1 cut(s) 22
TaqI TCGA 3 cut(s) 96, 204, 325
TasI AATT 2 cut(s) 403, 456
TatI WGTACW 1 cut(s) 191
TauI GCSGC 1 cut(s) 416
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 3 cut(s) 39, 290, 460
Tru9I TTAA 3 cut(s) 39, 290, 460
TscAI CASTG 1 cut(s) 119
TseI GCWGC 1 cut(s) 88
TspDTI ATGAA 4 cut(s) 44, 236, 281, 308
TspGWI ACGGA 1 cut(s) 339
TspRI CASTG 1 cut(s) 119
XmiI GTMKAC 2 cut(s) 16, 146
XspI CTAG 2 cut(s) 306, 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.