RLG00000014728

PI-PLC X domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0020571
Physical Location & Seq
Forward (+)
56870080 .. 56871025
946 bp
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UTR
Exon/CDS
Intron
RLM00000014728

Sequence Viewer

Length: 417 bp
ATGGAGATAATTTTGTTTGCAGATGGGGACAATGGGATGAAAGCTGGGACTTGCGGTAACCGTGGTGAATCGTCTCTGATGATGAACAAAACGAAGTCAACCATTTTAATGAACTACTTTTCTAGTAACGCTAATATCATTCGATCTTGCAATGATAATCCAGATCCTTTGCTAGATATGATCAAAACCTGCCAGGTAGCCTCGGCTAATCAATGGCCTAATTTCGTTGCCGCTGACTTTTACACCAAACGCAATGGTGGTGGCGCCTCAGCAGCTATGGATGTTGTACACGGACATTTGGCAGATAGGATTTTTGGAAAATGTCCAAAACCTCAGACGATTCCAGCTCCCCCTGCAACTACAAAAGCTCTCCCTGTACCTACAAAAGCGCCAAAGCCCCCAATCCATGCATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

139

Amino Acids

14.73

Weight (kDa)

8.98

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC_cat PF26178 8 - 100 4.6e-31 PI-PLC-like catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020571)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0257801
rosa_laevigata RLG00000014728
rosa_roxburghii Rroxscaffold_7G00208980
rosa_samantha Rh6AG084700 Rh6CG074100 Rh6DG071000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 197
AccB1I GGYRCC 1 cut(s) 263
AciI CCGC 2 cut(s) 54, 231
AclWI GGATC 1 cut(s) 158
AcyI GRCGYC 1 cut(s) 264
AfaI GTAC 2 cut(s) 288, 378
AjnI CCWGG 1 cut(s) 192
AluBI AGCT 4 cut(s) 44, 275, 347, 368
AluI AGCT 4 cut(s) 44, 275, 347, 368
Alw26I GTCTC 1 cut(s) 78
AlwI GGATC 1 cut(s) 158
AoxI GGCC 1 cut(s) 215
ApeKI GCWGC 1 cut(s) 272
AspLEI GCGC 2 cut(s) 266, 391
AsuHPI GGTGA 1 cut(s) 77
BanI GGYRCC 1 cut(s) 263
BbvCI CCTCAGC 1 cut(s) 268
BbvI GCAGC 1 cut(s) 284
BccI CCATC 1 cut(s) 17
BciT130I CCWGG 1 cut(s) 194
BclI TGATCA 1 cut(s) 180
BcoDI GTCTC 1 cut(s) 78
BfaI CTAG 2 cut(s) 123, 173
BfoI RGCGCY 2 cut(s) 267, 392
BfuAI ACCTGC 1 cut(s) 197
BisI GCNGC 2 cut(s) 231, 273
BlsI GCNGC 2 cut(s) 232, 274
Bme1390I CCNGG 1 cut(s) 194
BmiI GGNNCC 1 cut(s) 265
BmrFI CCNGG 1 cut(s) 194
Bpu10I CCTNAGC 1 cut(s) 268
BsaHI GRCGYC 1 cut(s) 264
BsaJI CCNNGG 2 cut(s) 61, 201
Bse3DI GCAATG 2 cut(s) 157, 259
BseBI CCWGG 1 cut(s) 194
BseDI CCNNGG 2 cut(s) 61, 201
BseGI GGATG 2 cut(s) 42, 286
BseMI GCAATG 2 cut(s) 157, 259
BseMII CTCAG 2 cut(s) 282, 347
BseXI GCAGC 1 cut(s) 284
BseYI CCCAGC 1 cut(s) 44
BshFI GGCC 1 cut(s) 217
BshNI GGYRCC 1 cut(s) 263
BslFI GGGAC 2 cut(s) 41, 61
BsmAI GTCTC 1 cut(s) 78
BsmBI CGTCTC 1 cut(s) 78
BsmFI GGGAC 2 cut(s) 41, 61
BsnI GGCC 1 cut(s) 217
Bsp1407I TGTACA 1 cut(s) 286
Bsp143I GATC 3 cut(s) 143, 163, 180
BspACI CCGC 2 cut(s) 54, 231
BspANI GGCC 1 cut(s) 217
BspCNI CTCAG 2 cut(s) 281, 346
BspLI GGNNCC 1 cut(s) 265
BspMI ACCTGC 1 cut(s) 197
BspPI GGATC 1 cut(s) 158
BspT107I GGYRCC 1 cut(s) 263
BsrDI GCAATG 2 cut(s) 157, 259
BsrGI TGTACA 1 cut(s) 286
BssECI CCNNGG 2 cut(s) 61, 201
BssMI GATC 3 cut(s) 143, 163, 180
BssNI GRCGYC 1 cut(s) 264
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 1 cut(s) 62
BstACI GRCGYC 1 cut(s) 264
BstAUI TGTACA 1 cut(s) 286
BstDEI CTNAG 2 cut(s) 268, 333
BstDSI CCRYGG 1 cut(s) 61
BstEII GGTNACC 1 cut(s) 56
BstF5I GGATG 2 cut(s) 42, 286
BstH2I RGCGCY 2 cut(s) 267, 392
BstHHI GCGC 2 cut(s) 266, 391
BstKTI GATC 3 cut(s) 146, 166, 183
BstMAI GTCTC 1 cut(s) 78
BstMBI GATC 3 cut(s) 143, 163, 180
BstMWI GCNNNNNNNGC 2 cut(s) 272, 353
BstNI CCWGG 1 cut(s) 194
BstPI GGTNACC 1 cut(s) 56
BstSCI CCNGG 1 cut(s) 192
BstV1I GCAGC 1 cut(s) 284
BstX2I RGATCY 1 cut(s) 163
BstYI RGATCY 1 cut(s) 163
BsuRI GGCC 1 cut(s) 217
BtgI CCRYGG 1 cut(s) 61
BtsCI GGATG 2 cut(s) 42, 286
BveI ACCTGC 1 cut(s) 197
CfoI GCGC 2 cut(s) 266, 391
Csp6I GTAC 2 cut(s) 287, 377
CspCI CAANNNNNGTGG 2 cut(s) 241, 276
CviAII CATG 1 cut(s) 407
CviJI RGCY 8 cut(s) 44, 200, 206, 217, 275, 347, 368, 397
CviKI_1 RGCY 8 cut(s) 44, 200, 206, 217, 275, 347, 368, 397
CviQI GTAC 2 cut(s) 287, 377
DdeI CTNAG 2 cut(s) 268, 333
DinI GGCGCC 1 cut(s) 265
DpnI GATC 3 cut(s) 145, 165, 182
DpnII GATC 3 cut(s) 143, 163, 180
Eco91I GGTNACC 1 cut(s) 56
EcoO65I GGTNACC 1 cut(s) 56
EcoRII CCWGG 1 cut(s) 192
EcoT22I ATGCAT 1 cut(s) 412
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
Esp3I CGTCTC 1 cut(s) 78
FaeI CATG 1 cut(s) 410
FaiI YATR 3 cut(s) 179, 278, 408
FaqI GGGAC 2 cut(s) 41, 61
FatI CATG 1 cut(s) 406
FbaI TGATCA 1 cut(s) 180
Fnu4HI GCNGC 2 cut(s) 231, 273
FokI GGATG 2 cut(s) 49, 293
Fsp4HI GCNGC 2 cut(s) 231, 273
FspBI CTAG 2 cut(s) 123, 173
GlaI GCGC 2 cut(s) 265, 390
GluI GCNGC 2 cut(s) 231, 273
GsaI CCCAGC 1 cut(s) 48
HaeII RGCGCY 2 cut(s) 267, 392
HaeIII GGCC 1 cut(s) 217
HhaI GCGC 2 cut(s) 266, 391
Hin1I GRCGYC 1 cut(s) 264
Hin1II CATG 1 cut(s) 410
Hin6I GCGC 2 cut(s) 264, 389
HinP1I GCGC 2 cut(s) 264, 389
HincII GTYRAC 1 cut(s) 99
HindII GTYRAC 1 cut(s) 99
HinfI GANTC 2 cut(s) 68, 340
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 2 cut(s) 99, 289
Hpy188I TCNGA 2 cut(s) 78, 336
Hpy188III TCNNGA 1 cut(s) 161
Hpy8I GTNNAC 2 cut(s) 99, 289
HpyCH4III ACNGT 1 cut(s) 62
HpyCH4V TGCA 4 cut(s) 20, 150, 356, 410
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 353
HpyF3I CTNAG 2 cut(s) 268, 333
Hsp92I GRCGYC 1 cut(s) 264
Hsp92II CATG 1 cut(s) 410
HspAI GCGC 2 cut(s) 264, 389
KasI GGCGCC 1 cut(s) 263
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 3 cut(s) 143, 163, 180
LmnI GCTCC 1 cut(s) 352
LpnPI CCDG 8 cut(s) 30, 174, 179, 202, 206, 357, 366, 387
Lsp1109I GCAGC 1 cut(s) 284
MaeI CTAG 2 cut(s) 123, 173
MaeIII GTNAC 2 cut(s) 56, 125
MalI GATC 3 cut(s) 145, 165, 182
MboI GATC 3 cut(s) 143, 163, 180
MflI RGATCY 1 cut(s) 163
MluCI AATT 2 cut(s) 9, 220
Mly113I GGCGCC 1 cut(s) 264
MnlI CCTC 3 cut(s) 211, 277, 342
Mph1103I ATGCAT 1 cut(s) 412
MseI TTAA 1 cut(s) 107
MslI CAYNNNNRTG 1 cut(s) 107
MspA1I CMGCKG 1 cut(s) 233
MspR9I CCNGG 1 cut(s) 194
MvaI CCWGG 1 cut(s) 194
MwoI GCNNNNNNNGC 2 cut(s) 272, 353
NarI GGCGCC 1 cut(s) 264
NdeII GATC 3 cut(s) 143, 163, 180
NlaIII CATG 1 cut(s) 410
NlaIV GGNNCC 1 cut(s) 265
NmeAIII GCCGAG 1 cut(s) 182
NsiI ATGCAT 1 cut(s) 412
PfeI GAWTC 2 cut(s) 68, 340
PkrI GCNGC 2 cut(s) 232, 274
PluTI GGCGCC 1 cut(s) 267
Psp6I CCWGG 1 cut(s) 192
PspEI GGTNACC 1 cut(s) 56
PspFI CCCAGC 1 cut(s) 44
PspGI CCWGG 1 cut(s) 192
PspN4I GGNNCC 1 cut(s) 265
PsuI RGATCY 1 cut(s) 163
RsaI GTAC 2 cut(s) 288, 378
RsaNI GTAC 2 cut(s) 287, 377
RseI CAYNNNNRTG 1 cut(s) 107
SaqAI TTAA 1 cut(s) 107
SatI GCNGC 2 cut(s) 231, 273
Sau3AI GATC 3 cut(s) 143, 163, 180
ScrFI CCNGG 1 cut(s) 194
SetI ASST 8 cut(s) 46, 191, 198, 277, 334, 349, 370, 382
SfoI GGCGCC 1 cut(s) 265
SmiMI CAYNNNNRTG 1 cut(s) 107
Sse9I AATT 2 cut(s) 9, 220
SsiI CCGC 2 cut(s) 54, 231
SspDI GGCGCC 1 cut(s) 263
SspMI CTAG 2 cut(s) 123, 173
StyD4I CCNGG 1 cut(s) 192
TaaI ACNGT 1 cut(s) 62
TaqI TCGA 1 cut(s) 142
TasI AATT 2 cut(s) 9, 220
TatI WGTACW 1 cut(s) 286
TauI GCSGC 1 cut(s) 233
TfiI GAWTC 2 cut(s) 68, 340
Tru1I TTAA 1 cut(s) 107
Tru9I TTAA 1 cut(s) 107
TseI GCWGC 1 cut(s) 272
TspDTI ATGAA 3 cut(s) 53, 98, 125
TspGWI ACGGA 1 cut(s) 306
XspI CTAG 2 cut(s) 123, 173
Zsp2I ATGCAT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.