RchiOBHm_Chr5g0009421

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0022138
Physical Location & Seq
Reverse (-)
6290023 .. 6291063
1041 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1041 bp
ATGATCGATGATTGTGATGCTCAAAATTCCATGGCCGTTGTAATGAGAATACGACCTCACTTACCGGACGACATTATCCGCCAAATTCTCTCACACCTGCCAACTAAATCCGCCATCCGCATGAGCATTCTTTCTAAGCAATTTGAAGGAGCATGGTGTTCCCTCCCTGTAATAGATCTCGACGAGGGAAAGCCACAGGCCAACCTTGATCGAGGTATACACTTCACCAACATCTTGAAAAGGTATTTGAAATTCCGTACTGAGAACAAAGACCGTCTGCATCCCCCCTTGGATAAATTTAGGCTTCGGATGGCGGTGATGTACCCGGATAGAGAAGATATTCTAGAAAAGTTGTTGACCAGTTTTGCACAAGAGAGAAGTGTCAAAGAGTTAGATCTCAGCTGTCCCACATTCACAAATATTGCACTAGAAAAGCGGCCGCAGTCATCCCCCCAAATACTCGTTAATATCAACTCTTTAACTAGTTTGAGTTTGGAGTCTGTGGGAATAGAGAATGATGTTACTCGTAGTGGTGATATACTTCTTCCATCTTTGAAGAACATGTCCCTCAAATCTATGCACCTGCAGTCTAGCTTTAAGTTTTTGTCCCATCTAATTTCCAGGTGCCCTGCCATTGAGTATTTGACATTGACTTCATGTACCGAATTCTTTTGGTTCGGTCGATCAGAGTCGATTACAGTTGCACGGTCTCGTACTCTCAAGCATTTGGAGATTAGAGATTGCAGTTTTTCAATATTCCATGTATCTGACTGTGAGGTTCTTGAATCTGTTACATTGGTTTCAAAATCTCCAAGAGTCATGTTAGCTTTGTACGGAGGCAGGAACTTGAAATATATCAACATCTGCTCAGAGAAGGCGACAGATATTCATATTGACCGTTGCAAAGACTCTGTGGAGGCCACAATGGATACTCCAAATCTTCAACTGTTTAGATTCAATGGTTTCGATGCGATGAAATGCATGAATGGAAGGCATTTATTGAGTAATTTCGTAATAAGCACAGAGATGCTGGCGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

346

Amino Acids

39.47

Weight (kDa)

8.02

Isoelectric Point (pI)

58.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 20 - 56 2.9e-08 F-box domain
LRR_At5g56370 PF24758 117 - 270 2.3e-10 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022138)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07881
rosa_chinensis RchiOBHm_Chr5g0009421
rosa_multiflora Rmu_sc0019489.1_g000001
rosa_samantha Rh5AG073400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 105, 591
Acc36I ACCTGC 2 cut(s) 105, 591
AccB1I GGYRCC 1 cut(s) 624
AccI GTMKAC 1 cut(s) 217
AciI CCGC 6 cut(s) 79, 111, 118, 314, 436, 440
AcoI YGGCCR 2 cut(s) 33, 437
AcsI RAATTY 5 cut(s) 25, 84, 251, 296, 665
AfaI GTAC 5 cut(s) 259, 323, 661, 715, 833
AflIII ACRYGT 1 cut(s) 561
AhlI ACTAGT 1 cut(s) 482
AjnI CCWGG 1 cut(s) 620
AluBI AGCT 4 cut(s) 402, 594, 827, 1038
AluI AGCT 4 cut(s) 402, 594, 827, 1038
Alw26I GTCTC 1 cut(s) 714
AoxI GGCC 4 cut(s) 33, 198, 437, 918
ApoI RAATTY 5 cut(s) 25, 84, 251, 296, 665
Asp700I GAANNNNTTC 1 cut(s) 339
AsuC2I CCSGG 1 cut(s) 326
AsuHPI GGTGA 3 cut(s) 217, 328, 545
BaeGI GKGCMC 1 cut(s) 629
BanI GGYRCC 1 cut(s) 624
BccI CCATC 4 cut(s) 122, 304, 556, 618
BceAI ACGGC 1 cut(s) 20
BciT130I CCWGG 1 cut(s) 622
BciVI GTATCC 1 cut(s) 922
BcnI CCSGG 1 cut(s) 326
BcoDI GTCTC 1 cut(s) 714
BcuI ACTAGT 1 cut(s) 482
BfaI CTAG 5 cut(s) 344, 428, 483, 591, 1039
BfmI CTRYAG 1 cut(s) 584
BfuAI ACCTGC 2 cut(s) 105, 591
BfuI GTATCC 1 cut(s) 922
BglII AGATCT 2 cut(s) 175, 394
BisI GCNGC 2 cut(s) 437, 440
BlsI GCNGC 2 cut(s) 438, 441
Bme1390I CCNGG 2 cut(s) 326, 622
BmiI GGNNCC 1 cut(s) 626
BmrFI CCNGG 2 cut(s) 326, 622
BmsI GCATC 4 cut(s) 7, 289, 958, 1017
BpuEI CTTGAG 1 cut(s) 704
BpuMI CCSGG 1 cut(s) 326
Bsa29I ATCGAT 1 cut(s) 6
BsaI GGTCTC 1 cut(s) 714
BsaJI CCNNGG 2 cut(s) 30, 288
BsaWI WCCGGW 1 cut(s) 64
Bse1I ACTGG 1 cut(s) 360
BseBI CCWGG 1 cut(s) 622
BseCI ATCGAT 1 cut(s) 6
BseDI CCNNGG 2 cut(s) 30, 288
BseGI GGATG 4 cut(s) 114, 280, 315, 446
BseMII CTCAG 3 cut(s) 252, 412, 882
BseNI ACTGG 1 cut(s) 360
BseSI GKGCMC 1 cut(s) 629
BseX3I CGGCCG 1 cut(s) 437
Bsh1285I CGRYCG 2 cut(s) 440, 682
BshFI GGCC 4 cut(s) 35, 200, 439, 920
BshNI GGYRCC 1 cut(s) 624
BshVI ATCGAT 1 cut(s) 6
BsiEI CGRYCG 2 cut(s) 440, 682
BsiSI CCGG 2 cut(s) 65, 326
BslFI GGGAC 3 cut(s) 390, 550, 592
BsmAI GTCTC 1 cut(s) 714
BsmFI GGGAC 3 cut(s) 390, 550, 592
BsmI GAATGC 1 cut(s) 126
BsnI GGCC 4 cut(s) 35, 200, 439, 920
Bso31I GGTCTC 1 cut(s) 714
Bsp1286I GDGCHC 1 cut(s) 629
Bsp143I GATC 5 cut(s) 3, 175, 208, 394, 683
Bsp19I CCATGG 1 cut(s) 30
BspACI CCGC 6 cut(s) 79, 111, 118, 314, 436, 440
BspANI GGCC 4 cut(s) 35, 200, 439, 920
BspCNI CTCAG 3 cut(s) 253, 411, 881
BspDI ATCGAT 1 cut(s) 6
BspLI GGNNCC 1 cut(s) 626
BspMAI CTGCAG 1 cut(s) 588
BspMI ACCTGC 2 cut(s) 105, 591
BspT107I GGYRCC 1 cut(s) 624
BspTNI GGTCTC 1 cut(s) 714
BsrI ACTGG 1 cut(s) 360
BssECI CCNNGG 2 cut(s) 30, 288
BssMI GATC 5 cut(s) 3, 175, 208, 394, 683
BssNAI GTATAC 1 cut(s) 218
BssT1I CCWWGG 2 cut(s) 30, 288
Bst1107I GTATAC 1 cut(s) 218
Bst2UI CCWGG 1 cut(s) 622
Bst4CI ACNGT 6 cut(s) 275, 700, 708, 773, 899, 948
BstC8I GCNNGC 2 cut(s) 1032, 1036
BstDEI CTNAG 4 cut(s) 135, 261, 398, 868
BstDSI CCRYGG 1 cut(s) 30
BstF5I GGATG 4 cut(s) 114, 280, 315, 446
BstKTI GATC 5 cut(s) 6, 178, 211, 397, 686
BstMAI GTCTC 1 cut(s) 714
BstMBI GATC 5 cut(s) 3, 175, 208, 394, 683
BstMCI CGRYCG 2 cut(s) 440, 682
BstNI CCWGG 1 cut(s) 622
BstNSI RCATGY 1 cut(s) 565
BstSCI CCNGG 2 cut(s) 324, 620
BstSFI CTRYAG 1 cut(s) 584
BstSLI GKGCMC 1 cut(s) 629
BstX2I RGATCY 2 cut(s) 175, 394
BstYI RGATCY 2 cut(s) 175, 394
BstZ17I GTATAC 1 cut(s) 218
BstZI CGGCCG 1 cut(s) 437
Bsu15I ATCGAT 1 cut(s) 6
BsuI GTATCC 1 cut(s) 922
BsuRI GGCC 4 cut(s) 35, 200, 439, 920
BsuTUI ATCGAT 1 cut(s) 6
BtgI CCRYGG 1 cut(s) 30
BtgZI GCGATG 1 cut(s) 986
BtsCI GGATG 4 cut(s) 114, 280, 315, 446
BveI ACCTGC 2 cut(s) 105, 591
Cac8I GCNNGC 2 cut(s) 1032, 1036
CciNI GCGGCCGC 1 cut(s) 437
ClaI ATCGAT 1 cut(s) 6
Csp6I GTAC 5 cut(s) 258, 322, 660, 714, 832
CviAII CATG 8 cut(s) 31, 121, 153, 562, 657, 761, 820, 982
CviQI GTAC 5 cut(s) 258, 322, 660, 714, 832
DdeI CTNAG 4 cut(s) 135, 261, 398, 868
DpnI GATC 5 cut(s) 5, 177, 210, 396, 685
DpnII GATC 5 cut(s) 3, 175, 208, 394, 683
EaeI YGGCCR 2 cut(s) 33, 437
EagI CGGCCG 1 cut(s) 437
EciI GGCGGA 2 cut(s) 68, 100
EclXI CGGCCG 1 cut(s) 437
Eco130I CCWWGG 2 cut(s) 30, 288
Eco31I GGTCTC 1 cut(s) 714
Eco52I CGGCCG 1 cut(s) 437
EcoRI GAATTC 1 cut(s) 665
EcoRII CCWGG 1 cut(s) 620
EcoT14I CCWWGG 2 cut(s) 30, 288
EcoT22I ATGCAT 1 cut(s) 983
ErhI CCWWGG 2 cut(s) 30, 288
FaeI CATG 8 cut(s) 34, 124, 156, 565, 660, 764, 823, 985
FaqI GGGAC 3 cut(s) 390, 550, 592
FatI CATG 8 cut(s) 30, 120, 152, 561, 656, 760, 819, 981
FblI GTMKAC 1 cut(s) 217
Fnu4HI GCNGC 2 cut(s) 437, 440
FokI GGATG 4 cut(s) 101, 267, 322, 433
Fsp4HI GCNGC 2 cut(s) 437, 440
FspBI CTAG 5 cut(s) 344, 428, 483, 591, 1039
GluI GCNGC 2 cut(s) 437, 440
HaeIII GGCC 4 cut(s) 35, 200, 439, 920
HapII CCGG 2 cut(s) 65, 326
Hin1II CATG 8 cut(s) 34, 124, 156, 565, 660, 764, 823, 985
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HinfI GANTC 6 cut(s) 497, 689, 785, 816, 908, 954
HpaII CCGG 2 cut(s) 65, 326
HphI GGTGA 3 cut(s) 217, 328, 545
Hpy166II GTNNAC 2 cut(s) 218, 357
Hpy188I TCNGA 4 cut(s) 309, 688, 769, 871
Hpy188III TCNNGA 4 cut(s) 179, 235, 344, 782
Hpy8I GTNNAC 2 cut(s) 218, 357
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 3 cut(s) 140, 868, 984
HpyCH4III ACNGT 6 cut(s) 275, 700, 708, 773, 899, 948
HpyCH4V TGCA 9 cut(s) 280, 368, 425, 580, 586, 704, 744, 903, 981
HpyF3I CTNAG 4 cut(s) 135, 261, 398, 868
Hsp92II CATG 8 cut(s) 34, 124, 156, 565, 660, 764, 823, 985
Kzo9I GATC 5 cut(s) 3, 175, 208, 394, 683
LmnI GCTCC 1 cut(s) 149
LweI GCATC 4 cut(s) 7, 289, 958, 1017
MaeI CTAG 5 cut(s) 344, 428, 483, 591, 1039
MaeIII GTNAC 2 cut(s) 520, 790
MalI GATC 5 cut(s) 5, 177, 210, 396, 685
MboI GATC 5 cut(s) 3, 175, 208, 394, 683
MboII GAAGA 4 cut(s) 347, 536, 568, 932
MflI RGATCY 2 cut(s) 175, 394
MhlI GDGCHC 1 cut(s) 629
MluCI AATT 8 cut(s) 25, 84, 140, 251, 296, 615, 665, 1006
MlyI GAGTC 4 cut(s) 506, 698, 825, 902
MnlI CCTC 8 cut(s) 66, 173, 178, 206, 578, 769, 830, 910
Mph1103I ATGCAT 1 cut(s) 983
MroXI GAANNNNTTC 1 cut(s) 339
MseI TTAA 3 cut(s) 465, 479, 597
MslI CAYNNNNRTG 2 cut(s) 119, 1025
MspA1I CMGCKG 1 cut(s) 402
MspI CCGG 2 cut(s) 65, 326
MspR9I CCNGG 2 cut(s) 326, 622
Mva1269I GAATGC 1 cut(s) 126
MvaI CCWGG 1 cut(s) 622
NciI CCSGG 1 cut(s) 326
NcoI CCATGG 1 cut(s) 30
NdeII GATC 5 cut(s) 3, 175, 208, 394, 683
NlaIII CATG 8 cut(s) 34, 124, 156, 565, 660, 764, 823, 985
NlaIV GGNNCC 1 cut(s) 626
NotI GCGGCCGC 1 cut(s) 437
NsiI ATGCAT 1 cut(s) 983
NspI RCATGY 1 cut(s) 565
PaqCI CACCTGC 2 cut(s) 105, 591
PciI ACATGT 1 cut(s) 561
PctI GAATGC 1 cut(s) 126
PdmI GAANNNNTTC 1 cut(s) 339
PfeI GAWTC 2 cut(s) 785, 954
PkrI GCNGC 2 cut(s) 438, 441
PleI GAGTC 4 cut(s) 505, 697, 824, 902
PpsI GAGTC 4 cut(s) 505, 697, 824, 902
PscI ACATGT 1 cut(s) 561
Psp6I CCWGG 1 cut(s) 620
PspGI CCWGG 1 cut(s) 620
PspN4I GGNNCC 1 cut(s) 626
PstI CTGCAG 1 cut(s) 588
PsuI RGATCY 2 cut(s) 175, 394
PvuII CAGCTG 1 cut(s) 402
RsaI GTAC 5 cut(s) 259, 323, 661, 715, 833
RsaNI GTAC 5 cut(s) 258, 322, 660, 714, 832
RseI CAYNNNNRTG 2 cut(s) 119, 1025
SaqAI TTAA 3 cut(s) 465, 479, 597
SatI GCNGC 2 cut(s) 437, 440
Sau3AI GATC 5 cut(s) 3, 175, 208, 394, 683
SchI GAGTC 4 cut(s) 506, 698, 825, 902
ScrFI CCNGG 2 cut(s) 326, 622
SduI GDGCHC 1 cut(s) 629
SfaNI GCATC 4 cut(s) 7, 289, 958, 1017
SfcI CTRYAG 1 cut(s) 584
SmiMI CAYNNNNRTG 2 cut(s) 119, 1025
SmlI CTYRAG 1 cut(s) 719
SmoI CTYRAG 1 cut(s) 719
SpeI ACTAGT 1 cut(s) 482
Sse9I AATT 8 cut(s) 25, 84, 140, 251, 296, 615, 665, 1006
SsiI CCGC 6 cut(s) 79, 111, 118, 314, 436, 440
SspI AATATT 2 cut(s) 421, 756
SspMI CTAG 5 cut(s) 344, 428, 483, 591, 1039
StyD4I CCNGG 2 cut(s) 324, 620
StyI CCWWGG 2 cut(s) 30, 288
TaaI ACNGT 6 cut(s) 275, 700, 708, 773, 899, 948
TaqI TCGA 6 cut(s) 6, 180, 211, 682, 692, 966
TaqII GACCGA 1 cut(s) 668
TasI AATT 8 cut(s) 25, 84, 140, 251, 296, 615, 665, 1006
TauI GCSGC 2 cut(s) 439, 442
TfiI GAWTC 2 cut(s) 785, 954
Tru1I TTAA 3 cut(s) 465, 479, 597
Tru9I TTAA 3 cut(s) 465, 479, 597
TspDTI ATGAA 4 cut(s) 645, 878, 989, 998
TspGWI ACGGA 2 cut(s) 245, 849
XapI RAATTY 5 cut(s) 25, 84, 251, 296, 665
XbaI TCTAGA 1 cut(s) 343
XceI RCATGY 1 cut(s) 565
XmiI GTMKAC 1 cut(s) 217
XmnI GAANNNNTTC 1 cut(s) 339
XspI CTAG 5 cut(s) 344, 428, 483, 591, 1039
Zsp2I ATGCAT 1 cut(s) 983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.