Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0020558

Physical Location & Seq

Genomic Coordinates Reverse (-)

33669675 .. 33673306

Gene Structure 3632 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3060 bp

ATGTCTGATTCTGAAAGCATGCATGATCCCAGCAGTTGGCTTATTAGCAAAAAGAAAAGCAAATGGCGTTCTGCTTTTTTCACCGTCTACTGCTCTAGAGCCTTCCTCTCTTTATTAAAACCTTCCTTGCTCAAAATCACAGAAACCAAATCCCTGTCTGGTTCTCTCTCTCCTGTCAATACCACTGCCGATTCAGATCCAAACCTCGATGACTTCGGTGTTGACCAAACAGCTCTTACTCAACTTGTGAAGAACAAAAATCTCTACCAGCTGCAAGAGTTGGGAGGAGTTGAGGGAGTAATATCCCACCTCAAATCTGATGCAGTGAGTGGAATTCCTAGTGTTGATGCTGAGGACATTCTCAAAAGAATCAAAGCATTTGGTTCCAACGTTTATCAAAAAGCACCAACAAGAAGGTTTTTCCATTTTGTGTTGGACGCCTTCAAAGACCTAACAATTCTCATTCTATTAGGCTGTGCAGCGCTCTCTCTTTGTGTTGGCATCAAAGAGCATGGATTCAGACAAGGTTGGATTGATGGTGGGAGCATATTGCTTGCAGTCATTTTGGCCATTTCTGTGTCAGCAACCGGCCCTTACTTGCAGAGCAGACATTTGGTCCACAAGAATATCCAAATTGAGGCTGTGAGAGGTGGCAAGAAGCAAAAGATTTCTCTTTCTGAAGCTGTCGTTGGTGATGTAATTTTCTTGAACTGCGGAGATCAGGTGCCGGCTGATGGGTTACTTGTAGAAGGCTATTCACTGCAAGTAGACCAATCAAGCATGTCAGGACAAAGTGATGATATTGTTGAAATTAGCCATGACCAGAATCCATTCCTGTTTTCTGGGACAAAGATTACACATGGGTATGCTCGAATGATTGTCATATCCGTCGGAATGAACACAAAACGGGGTGAAATGACTAACCAAATCAGCCAAGACACTAGTGAAAAGACACTACTGCAAGCACGTCTGGAGAAGCTAACTTCACTGATAGGTAAAGTCGGTCTGGTGGTTGCTTTCGCTGTTCTTGTAGTGTTGTTAGTTCGATACTTCACAGGGCATACGGAGGATGAGAATGGAGACAAAGAGTTCAATTCCAGCAGCATTGAAGTTGATGACATGATAAGTGCTGTAACTGGGATTATAATGGCTGCCGTTTCAATTGTCGTCGTGGCAATTCCAGAAGGTCTGCCATTAGCACTAACTCTTACTGTTGCCTATTCTATGAAGAAAATGATGGCTGACAAAGCAACGGTTCTGAGGCTCTCTGCTGCTGAGAATATGGCATTTACCACCACAATCTGTACTGACAAAACAGGTACTCTCACAATGAACCAGATGAAGGTGACCAAGTTTTGGATAGGTGAAAATAGTTTGGAACAAGAAGCTTATTCATCAAAAATTTCTCATTCTGTTCTTAATTTGATCCGAGAAGGGGTTGCTTTGAATACAAGTGGTAGTGTGTACAAGCGTAGCCTGGATTCAGAAATTGAGATCTCAGGCAGTCCAACTGAAAAGGCTATTCTATGGTGGGCAGTATATGGGTCACAGATGGATGTGGAGAAAGTAACGAAGAGTTGTAGCGTTCTCCAATTTGAGGCCTTCAACTCACAGAACAAGCGAAGTGGGGTTTTGATTAAGAGGAAGGAAGACAGCACAATTCATGTACATTGGAAAGGAGCTGCAGAGATCATACTAGGAATGTGCTCTAGTTACTACGAACCCTCTGGAGTCGTCAAACAAATGGATGATAATGAAAAAGGGAAATTTCAGCAGATTATTCAAGGCATGGCAGCTAGCAGACTCCGATGCATTGCATTTGCACATAAAGAAATTCCAGCAGATCAATATCGACTGGACCTAAAACAAAAAATTATGCTACAAGAAGATGGATTTACCCTGTTGGGACTCATAGGTCTTAAAGATCCGTGCCGTCCTGGAGTGTGGAAAGCAGTTAAAGATTGTCAAAACGCCGGGGTGAACATCAAAATGATCACAGGGGATAATGTTTTCACTGCACAAGCCATTGCCACAGAATGTGGGATACTCAAGCCTGGTCAGGACATCTTCAGTGGAGCAGTGATAGAAGGTGCTGTTTTTCAAAGCTACTCTGACGAGGATAAACTGTTGAATGTGGACAATATCTGTGTAATGGCAAGGTCCTCTGCTCTTGATAAGCTTACAATGGTGCAATGCTTGAAGAAGAAACGGCATGTAGTTGCAGTGACCGGTGATGGCGCTAGTGATGCACCTGCATTAAAAGAAGCTGATATAGGACTATCTTTGGGAATTAAAGGGACACAAGTTGCCAAAGAAAGCTCAGATATCATAATCAGCGATGATAACTTTGATTCGGTGGTGAATGTTTTGGCATGTGGAAGATGTGTTTATCACAATACTCAGAAGCTCATTCAATTCCAGCTCACAGCAAATTTTACTGGTCTTGTAATCAACTTTGCAGCAGCAGCTTCATCAAGAGCAGTCCCATTTACAGTTGTTCGTTCAATTTGGTTGAACTTGATTATGGACACACTGCTAGCTCTGGCTCTTTCCAAGGACAAACCCACAAAAGAGCTCATGGAGAAGCCGCCGGTGGGCAGAACTAATCCCCTCATTACCAACATCATGTGGAGGAACCTCTCGGCTCATGCTCTGTACCAGACGACAGTCCTTTTGGTATTACAATTCAAGGGCAAAGCAATCTTTGGTGTGGATGATAAGGTACTGGGTACCTTGGTATTTAACACTTATCTTCTTTGCCAGGTGTTCAATGAGTTTAATGCAAGGAAGCTTGAGAAGAAGGATATCTTTAGGGGTTTACAGACAGACAAATTGTTCATGGGGGTCATTACGGTGGCAATTGTTCTACAGGTGGTGACGCTGGAACTTTTGAGCAAATATTTGGATACAGGGAGGTTGAACTGCGGACAATGGGGACTATGTCTGGGAATTGCAGCCATGTCTTGGCCGATCGGATGGGTTGTTAAGTGTATACCCGTGCCACAGAAACCCATTCTCCGCTACCTGCTCGAGTTGAAGTTGGAAAGCAAACAAGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1019

Amino Acids

111.62

Weight (kDa)

8.08

Isoelectric Point (pI)

33.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	94 - 163	9.9e-13	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	213 - 310	1.7e-17	P-type ATPase actuator domain
Cation_ATPase	PF13246	474 - 646	3.7e-27	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	625 - 757	1.7e-10	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	828 - 997	6.5e-37	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0020558)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr5g0038931
rosa_laevigata	RLG00000033881
rosa_multiflora	Rmu_sc0000533.1_g000080
rosa_rugosa	Rorug05G0175300
rosa_samantha	Rh5BG266800
rosa_wichuraiana	Rw5G024460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1145
AarI	CACCTGC	1 cut(s)	2260
AasI	GACNNNNNNGTC	1 cut(s)	1914
Acc36I	ACCTGC	2 cut(s)	2260, 3033
Acc65I	GGTACC	1 cut(s)	2729
AccB1I	GGYRCC	2 cut(s)	724, 2729
AccB7I	CCANNNNNTGG	3 cut(s)	36, 1356, 2964
AccI	GTMKAC	3 cut(s)	87, 768, 2992
AciI	CCGC	4 cut(s)	714, 2588, 2925, 3019
AclI	AACGTT	1 cut(s)	390
AclWI	GGATC	4 cut(s)	20, 191, 1420, 1919
AcoI	YGGCCR	2 cut(s)	567, 2966
AcsI	RAATTY	5 cut(s)	333, 1401, 1766, 1833, 2431
AcuI	CTGAAG	2 cut(s)	699, 2053
AcyI	GRCGYC	1 cut(s)	438
AfaI	GTAC	7 cut(s)	1306, 1321, 1466, 1668, 2657, 2724, 2731
AfeI	AGCGCT	1 cut(s)	483
AfiI	CCNNNNNNNGG	9 cut(s)	36, 637, 734, 1342, 1356, 1435, 1597, 2594, 2964
AgeI	ACCGGT	1 cut(s)	2228
AhdI	GACNNNNNGTC	1 cut(s)	614
AhlI	ACTAGT	1 cut(s)	941
AjiI	CACGTC	1 cut(s)	968
AjnI	CCWGG	4 cut(s)	1476, 1936, 2053, 2760
AjuI	GAANNNNNNNTTGG	2 cut(s)	672, 704
Alw21I	GWGCWC	2 cut(s)	1709, 2577
Alw26I	GTCTC	1 cut(s)	1074
AlwI	GGATC	4 cut(s)	20, 191, 1420, 1919
Ama87I	CYCGRG	1 cut(s)	3029
Aor51HI	AGCGCT	1 cut(s)	483
AoxI	GGCC	4 cut(s)	567, 589, 1599, 2966
ApoI	RAATTY	5 cut(s)	333, 1401, 1766, 1833, 2431
ArsI	GACNNNNNNTTYG	2 cut(s)	2656, 2688
AsiGI	ACCGGT	1 cut(s)	2228
Asp700I	GAANNNNTTC	1 cut(s)	830
Asp718I	GGTACC	1 cut(s)	2729
AspLEI	GCGC	2 cut(s)	484, 2240
AspS9I	GGNCC	4 cut(s)	590, 616, 1858, 2160
AsuC2I	CCSGG	1 cut(s)	1975
AsuHPI	GGTGA	9 cut(s)	73, 704, 923, 1357, 1376, 1990, 2243, 2371, 2887
AsuNHI	GCTAGC	2 cut(s)	1796, 2536
AvaI	CYCGRG	1 cut(s)	3029
AvaII	GGWCC	3 cut(s)	616, 1858, 2160
BaeI	ACNNNNGTAYC	2 cut(s)	2721, 2754
BalI	TGGCCA	1 cut(s)	569
BanI	GGYRCC	2 cut(s)	724, 2729
BanII	GRGCYC	1 cut(s)	2577
BbsI	GAAGAC	1 cut(s)	1656
Bbv12I	GWGCWC	2 cut(s)	1709, 2577
BbvCI	CCTCAGC	1 cut(s)	351
BccI	CCATC	7 cut(s)	530, 728, 1231, 1546, 1883, 2228, 2970
BceAI	ACGGC	3 cut(s)	1139, 1917, 2225
BciT130I	CCWGG	4 cut(s)	1478, 1938, 2055, 2762
BciVI	GTATCC	2 cut(s)	2037, 2899
BclI	TGATCA	1 cut(s)	1992
BcnI	CCSGG	1 cut(s)	1975
BcoDI	GTCTC	1 cut(s)	1074
BcuI	ACTAGT	1 cut(s)	941
BfaI	CTAG	8 cut(s)	96, 339, 942, 1697, 1710, 1797, 2241, 2537
BfmI	CTRYAG	2 cut(s)	1683, 2867
BfoI	RGCGCY	2 cut(s)	485, 2241
BfuAI	ACCTGC	2 cut(s)	2260, 3033
BfuI	GTATCC	2 cut(s)	2037, 2899
BglII	AGATCT	1 cut(s)	1494
Bme1390I	CCNGG	5 cut(s)	1478, 1938, 1975, 2055, 2762
Bme18I	GGWCC	3 cut(s)	616, 1858, 2160
BmeRI	GACNNNNNGTC	1 cut(s)	614
BmeT110I	CYCGRG	1 cut(s)	3029
BmgBI	CACGTC	1 cut(s)	968
BmgT120I	GGNCC	4 cut(s)	590, 616, 1858, 2160
BmiI	GGNNCC	4 cut(s)	385, 726, 2636, 2731
BmrFI	CCNGG	5 cut(s)	1478, 1938, 1975, 2055, 2762
BmrI	ACTGGG	2 cut(s)	1146, 2735
BmsI	GCATC	5 cut(s)	310, 337, 510, 1799, 2236
BmtI	GCTAGC	2 cut(s)	1800, 2540
BmuI	ACTGGG	2 cut(s)	1146, 2735
BoxI	GACNNNNGTC	1 cut(s)	2666
BpiI	GAAGAC	1 cut(s)	1656
BplI	GAGNNNNNCTC	2 cut(s)	90, 122
BpmI	CTGGAG	3 cut(s)	992, 1749, 1959
Bpu10I	CCTNAGC	1 cut(s)	351
BpuEI	CTTGAG	2 cut(s)	2033, 2813
BpuMI	CCSGG	1 cut(s)	1975
BsaBI	GATNNNNATC	2 cut(s)	195, 1848
BsaHI	GRCGYC	1 cut(s)	438
BsaJI	CCNNGG	3 cut(s)	1974, 2553, 2733
BsaWI	WCCGGW	1 cut(s)	2228
BsaXI	ACNNNNNCTCC	4 cut(s)	708, 738, 3000, 3030
Bsc4I	CCNNNNNNNGG	9 cut(s)	36, 637, 734, 1342, 1356, 1435, 1597, 2594, 2964
Bse118I	RCCGGY	4 cut(s)	587, 727, 2228, 2590
Bse1I	ACTGG	4 cut(s)	1141, 1860, 2443, 2730
Bse3DI	GCAATG	3 cut(s)	1812, 2025, 2198
Bse8I	GATNNNNATC	2 cut(s)	195, 1848
BseBI	CCWGG	4 cut(s)	1478, 1938, 2055, 2762
BseDI	CCNNGG	3 cut(s)	1974, 2553, 2733
BseGI	GGATG	5 cut(s)	1075, 1561, 1753, 2719, 2981
BseJI	GATNNNNATC	2 cut(s)	195, 1848
BseLI	CCNNNNNNNGG	9 cut(s)	36, 637, 734, 1342, 1356, 1435, 1597, 2594, 2964
BseMI	GCAATG	3 cut(s)	1812, 2025, 2198
BseMII	CTCAG	6 cut(s)	342, 1250, 1266, 1512, 2334, 2414
BseNI	ACTGG	4 cut(s)	1141, 1860, 2443, 2730
BseRI	GAGGAG	1 cut(s)	300
BseYI	CCCAGC	1 cut(s)	29
BsgI	GTGCAG	2 cut(s)	498, 2001
Bsh1285I	CGRYCG	1 cut(s)	2973
BshFI	GGCC	4 cut(s)	569, 591, 1601, 2968
BshNI	GGYRCC	2 cut(s)	724, 2729
BshTI	ACCGGT	1 cut(s)	2228
BsiEI	CGRYCG	1 cut(s)	2973
BsiHKAI	GWGCWC	2 cut(s)	1709, 2577
BsiHKCI	CYCGRG	1 cut(s)	3029
BsiSI	CCGG	5 cut(s)	588, 728, 1974, 2229, 2591
BslFI	GGGAC	5 cut(s)	859, 1920, 2311, 2468, 2949
BslI	CCNNNNNNNGG	9 cut(s)	36, 637, 734, 1342, 1356, 1435, 1597, 2594, 2964
BsmAI	GTCTC	1 cut(s)	1074
BsmFI	GGGAC	5 cut(s)	859, 1920, 2311, 2468, 2949
BsnI	GGCC	4 cut(s)	569, 591, 1601, 2968
BsoBI	CYCGRG	1 cut(s)	3029
Bsp1286I	GDGCHC	2 cut(s)	1709, 2577
Bsp1407I	TGTACA	2 cut(s)	1464, 1666
BspACI	CCGC	4 cut(s)	714, 2588, 2925, 3019
BspANI	GGCC	4 cut(s)	569, 591, 1601, 2968
BspCNI	CTCAG	6 cut(s)	343, 1251, 1267, 1511, 2333, 2413
BspLI	GGNNCC	4 cut(s)	385, 726, 2636, 2731
BspMAI	CTGCAG	1 cut(s)	1687
BspMI	ACCTGC	2 cut(s)	2260, 3033
BspOI	GCTAGC	2 cut(s)	1800, 2540
BspPI	GGATC	4 cut(s)	20, 191, 1420, 1919
BspT107I	GGYRCC	2 cut(s)	724, 2729
BsrDI	GCAATG	3 cut(s)	1812, 2025, 2198
BsrFI	RCCGGY	4 cut(s)	587, 727, 2228, 2590
BsrGI	TGTACA	2 cut(s)	1464, 1666
BsrI	ACTGG	4 cut(s)	1141, 1860, 2443, 2730
BssAI	RCCGGY	4 cut(s)	587, 727, 2228, 2590
BssECI	CCNNGG	3 cut(s)	1974, 2553, 2733
BssNAI	GTATAC	1 cut(s)	2993
BssNI	GRCGYC	1 cut(s)	438
BssT1I	CCWWGG	2 cut(s)	2553, 2733
Bst1107I	GTATAC	1 cut(s)	2993
Bst2UI	CCWGG	4 cut(s)	1478, 1938, 2055, 2762
Bst4CI	ACNGT	7 cut(s)	85, 1213, 1255, 2127, 2494, 2668, 2854
Bst6I	CTCTTC	1 cut(s)	1568
BstACI	GRCGYC	1 cut(s)	438
BstAUI	TGTACA	2 cut(s)	1464, 1666
BstC8I	GCNNGC	6 cut(s)	20, 555, 729, 963, 1798, 2538
BstDEI	CTNAG	6 cut(s)	351, 1259, 1275, 1498, 2320, 2400
BstEII	GGTNACC	1 cut(s)	1345
BstF5I	GGATG	5 cut(s)	1075, 1561, 1753, 2719, 2981
BstH2I	RGCGCY	2 cut(s)	485, 2241
BstHHI	GCGC	2 cut(s)	484, 2240
BstMAI	GTCTC	1 cut(s)	1074
BstMCI	CGRYCG	1 cut(s)	2973
BstMWI	GCNNNNNNNGC	3 cut(s)	1247, 2246, 2465
BstNI	CCWGG	4 cut(s)	1478, 1938, 2055, 2762
BstNSI	RCATGY	4 cut(s)	22, 784, 2216, 2376
BstPAI	GACNNNNGTC	1 cut(s)	2666
BstPI	GGTNACC	1 cut(s)	1345
BstSCI	CCNGG	5 cut(s)	1476, 1936, 1973, 2053, 2760
BstSFI	CTRYAG	2 cut(s)	1683, 2867
BstV2I	GAAGAC	1 cut(s)	1656
BstX2I	RGATCY	3 cut(s)	196, 1494, 1924
BstYI	RGATCY	3 cut(s)	196, 1494, 1924
BstZ17I	GTATAC	1 cut(s)	2993
BsuI	GTATCC	2 cut(s)	2037, 2899
BsuRI	GGCC	4 cut(s)	569, 591, 1601, 2968
BtgZI	GCGATG	1 cut(s)	2352
BtrI	CACGTC	1 cut(s)	968
BtsCI	GGATG	5 cut(s)	1075, 1561, 1753, 2719, 2981
BtsI	GCAGTG	7 cut(s)	183, 330, 758, 2013, 2085, 2229, 2531
BtsIMutI	CAGTG	9 cut(s)	183, 330, 758, 986, 2013, 2077, 2085, 2229, 2531
BveI	ACCTGC	2 cut(s)	2260, 3033
Cac8I	GCNNGC	6 cut(s)	20, 555, 729, 963, 1798, 2538
CfoI	GCGC	2 cut(s)	484, 2240
Cfr10I	RCCGGY	4 cut(s)	587, 727, 2228, 2590
Cfr13I	GGNCC	4 cut(s)	590, 616, 1858, 2160
CseI	GACGC	2 cut(s)	446, 2887
Csp6I	GTAC	7 cut(s)	1305, 1320, 1465, 1667, 2656, 2723, 2730
CspAI	ACCGGT	1 cut(s)	2228
CspCI	CAANNNNNGTGG	2 cut(s)	1606, 1641
CviQI	GTAC	7 cut(s)	1305, 1320, 1465, 1667, 2656, 2723, 2730
DdeI	CTNAG	6 cut(s)	351, 1259, 1275, 1498, 2320, 2400
DrdI	GACNNNNNNGTC	1 cut(s)	1914
DriI	GACNNNNNGTC	1 cut(s)	614
DseDI	GACNNNNNNGTC	1 cut(s)	1914
EaeI	YGGCCR	2 cut(s)	567, 2966
Eam1104I	CTCTTC	1 cut(s)	1568
Eam1105I	GACNNNNNGTC	1 cut(s)	614
EarI	CTCTTC	1 cut(s)	1568
Ecl136II	GAGCTC	1 cut(s)	2575
Eco130I	CCWWGG	2 cut(s)	2553, 2733
Eco147I	AGGCCT	1 cut(s)	1601
Eco24I	GRGCYC	1 cut(s)	2577
Eco32I	GATATC	2 cut(s)	2326, 2806
Eco47I	GGWCC	3 cut(s)	616, 1858, 2160
Eco47III	AGCGCT	1 cut(s)	483
Eco53kI	GAGCTC	1 cut(s)	2575
Eco57I	CTGAAG	2 cut(s)	699, 2053
Eco88I	CYCGRG	1 cut(s)	3029
Eco91I	GGTNACC	1 cut(s)	1345
EcoICRI	GAGCTC	1 cut(s)	2575
EcoO109I	RGGNCCY	1 cut(s)	2160
EcoO65I	GGTNACC	1 cut(s)	1345
EcoRI	GAATTC	1 cut(s)	333
EcoRII	CCWGG	4 cut(s)	1476, 1936, 2053, 2760
EcoRV	GATATC	2 cut(s)	2326, 2806
EcoT14I	CCWWGG	2 cut(s)	2553, 2733
EcoT22I	ATGCAT	2 cut(s)	24, 1814
EcoT38I	GRGCYC	1 cut(s)	2577
ErhI	CCWWGG	2 cut(s)	2553, 2733
FalI	AAGNNNNNCTT	2 cut(s)	2792, 2824
FaqI	GGGAC	5 cut(s)	859, 1920, 2311, 2468, 2949
FbaI	TGATCA	1 cut(s)	1992
FblI	GTMKAC	3 cut(s)	87, 768, 2992
FokI	GGATG	5 cut(s)	1082, 1568, 1760, 2726, 2988
FriOI	GRGCYC	1 cut(s)	2577
FspBI	CTAG	8 cut(s)	96, 339, 942, 1697, 1710, 1797, 2241, 2537
GlaI	GCGC	2 cut(s)	483, 2239
GsaI	CCCAGC	1 cut(s)	33
GsuI	CTGGAG	3 cut(s)	992, 1749, 1959
HaeII	RGCGCY	2 cut(s)	485, 2241
HaeIII	GGCC	4 cut(s)	569, 591, 1601, 2968
HapII	CCGG	5 cut(s)	588, 728, 1974, 2229, 2591
HgaI	GACGC	2 cut(s)	446, 2887
HhaI	GCGC	2 cut(s)	484, 2240
Hin1I	GRCGYC	1 cut(s)	438
Hin6I	GCGC	2 cut(s)	482, 2238
HinP1I	GCGC	2 cut(s)	482, 2238
HincII	GTYRAC	1 cut(s)	223
HindII	GTYRAC	1 cut(s)	223
HindIII	AAGCTT	3 cut(s)	1386, 2177, 2789
HpaII	CCGG	5 cut(s)	588, 728, 1974, 2229, 2591
HphI	GGTGA	9 cut(s)	73, 704, 923, 1357, 1376, 1990, 2243, 2371, 2887
Hpy166II	GTNNAC	9 cut(s)	88, 223, 619, 769, 1465, 1981, 2137, 2819, 2993
Hpy188III	TCNNGA	9 cut(s)	96, 706, 786, 971, 1181, 1728, 2060, 2171, 2475
Hpy8I	GTNNAC	9 cut(s)	88, 223, 619, 769, 1465, 1981, 2137, 2819, 2993
Hpy99I	CGWCG	2 cut(s)	893, 1172
HpyCH4III	ACNGT	7 cut(s)	85, 1213, 1255, 2127, 2494, 2668, 2854
HpyCH4IV	ACGT	2 cut(s)	390, 967
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1247, 2246, 2465
HpyF3I	CTNAG	6 cut(s)	351, 1259, 1275, 1498, 2320, 2400
HpySE526I	ACGT	2 cut(s)	390, 967
Hsp92I	GRCGYC	1 cut(s)	438
HspAI	GCGC	2 cut(s)	482, 2238
KpnI	GGTACC	1 cut(s)	2733
KroI	GCCGGC	1 cut(s)	727
KroNI	GCCGGC	1 cut(s)	729
Ksp22I	TGATCA	1 cut(s)	1992
LmnI	GCTCC	3 cut(s)	543, 1679, 2075
LweI	GCATC	5 cut(s)	310, 337, 510, 1799, 2236
MaeI	CTAG	8 cut(s)	96, 339, 942, 1697, 1710, 1797, 2241, 2537
MaeII	ACGT	2 cut(s)	390, 967
MaeIII	GTNAC	8 cut(s)	738, 1132, 1345, 1545, 1567, 1712, 2224, 2875
MfeI	CAATTG	2 cut(s)	1161, 2859
MflI	RGATCY	3 cut(s)	196, 1494, 1924
MhlI	GDGCHC	2 cut(s)	1709, 2577
MlsI	TGGCCA	1 cut(s)	569
MluNI	TGGCCA	1 cut(s)	569
MlyI	GAGTC	3 cut(s)	1740, 1797, 1902
MmeI	TCCRAC	6 cut(s)	411, 414, 509, 871, 1532, 3021
Mox20I	TGGCCA	1 cut(s)	569
Mph1103I	ATGCAT	2 cut(s)	24, 1814
MroNI	GCCGGC	1 cut(s)	727
MroXI	GAANNNNTTC	1 cut(s)	830
MscI	TGGCCA	1 cut(s)	569
MslI	CAYNNNNRTG	4 cut(s)	575, 864, 1988, 2852
Msp20I	TGGCCA	1 cut(s)	569
MspA1I	CMGCKG	1 cut(s)	271
MspI	CCGG	5 cut(s)	588, 728, 1974, 2229, 2591
MspR9I	CCNGG	5 cut(s)	1478, 1938, 1975, 2055, 2762
MunI	CAATTG	2 cut(s)	1161, 2859
MvaI	CCWGG	4 cut(s)	1478, 1938, 2055, 2762
MwoI	GCNNNNNNNGC	3 cut(s)	1247, 2246, 2465
NaeI	GCCGGC	1 cut(s)	729
NciI	CCSGG	1 cut(s)	1975
NgoMIV	GCCGGC	1 cut(s)	727
NheI	GCTAGC	2 cut(s)	1796, 2536
NlaIV	GGNNCC	4 cut(s)	385, 726, 2636, 2731
NmeAIII	GCCGAG	1 cut(s)	2621
NmuCI	GTSAC	4 cut(s)	1345, 1545, 2224, 2875
NsiI	ATGCAT	2 cut(s)	24, 1814
NspI	RCATGY	4 cut(s)	22, 784, 2216, 2376
PaeI	GCATGC	1 cut(s)	22
PaeR7I	CTCGAG	1 cut(s)	3029
PaqCI	CACCTGC	1 cut(s)	2260
PceI	AGGCCT	1 cut(s)	1601
PdiI	GCCGGC	1 cut(s)	729
PdmI	GAANNNNTTC	1 cut(s)	830
PfeI	GAWTC	7 cut(s)	8, 191, 369, 516, 826, 1481, 2351
PflFI	GACNNNGTC	1 cut(s)	2940
PflMI	CCANNNNNTGG	3 cut(s)	36, 1356, 2964
PfoI	TCCNGGA	1 cut(s)	1936
PinAI	ACCGGT	1 cut(s)	2228
Ple19I	CGATCG	1 cut(s)	2973
PleI	GAGTC	3 cut(s)	1739, 1797, 1902
PpsI	GAGTC	3 cut(s)	1739, 1797, 1902
PpuMI	RGGWCCY	1 cut(s)	2160
PshAI	GACNNNNGTC	1 cut(s)	2666
PsiI	TTATAA	1 cut(s)	1145
Psp124BI	GAGCTC	1 cut(s)	2577
Psp1406I	AACGTT	1 cut(s)	390
Psp5II	RGGWCCY	1 cut(s)	2160
Psp6I	CCWGG	4 cut(s)	1476, 1936, 2053, 2760
PspEI	GGTNACC	1 cut(s)	1345
PspFI	CCCAGC	1 cut(s)	29
PspGI	CCWGG	4 cut(s)	1476, 1936, 2053, 2760
PspN4I	GGNNCC	4 cut(s)	385, 726, 2636, 2731
PspPI	GGNCC	4 cut(s)	590, 616, 1858, 2160
PspPPI	RGGWCCY	1 cut(s)	2160
PspXI	VCTCGAGB	1 cut(s)	3029
PstI	CTGCAG	1 cut(s)	1687
PsuI	RGATCY	3 cut(s)	196, 1494, 1924
PsyI	GACNNNGTC	1 cut(s)	2940
PvuI	CGATCG	1 cut(s)	2973
PvuII	CAGCTG	1 cut(s)	271
RsaI	GTAC	7 cut(s)	1306, 1321, 1466, 1668, 2657, 2724, 2731
RsaNI	GTAC	7 cut(s)	1305, 1320, 1465, 1667, 2656, 2723, 2730
RseI	CAYNNNNRTG	4 cut(s)	575, 864, 1988, 2852
SacI	GAGCTC	1 cut(s)	2577
Sau96I	GGNCC	4 cut(s)	590, 616, 1858, 2160
SchI	GAGTC	3 cut(s)	1740, 1797, 1902
ScrFI	CCNGG	5 cut(s)	1478, 1938, 1975, 2055, 2762
SduI	GDGCHC	2 cut(s)	1709, 2577
SfaNI	GCATC	5 cut(s)	310, 337, 510, 1799, 2236
SfcI	CTRYAG	2 cut(s)	1683, 2867
Sfr274I	CTCGAG	1 cut(s)	3029
SgrAI	CRCCGGYG	1 cut(s)	2590
SinI	GGWCC	3 cut(s)	616, 1858, 2160
SlaI	CTCGAG	1 cut(s)	3029
SmiMI	CAYNNNNRTG	4 cut(s)	575, 864, 1988, 2852
SmlI	CTYRAG	3 cut(s)	2048, 2792, 3029
SmoI	CTYRAG	3 cut(s)	2048, 2792, 3029
SpeI	ACTAGT	1 cut(s)	941
SphI	GCATGC	1 cut(s)	22
SseBI	AGGCCT	1 cut(s)	1601
SsiI	CCGC	4 cut(s)	714, 2588, 2925, 3019
SspI	AATATT	1 cut(s)	2900
SspMI	CTAG	8 cut(s)	96, 339, 942, 1697, 1710, 1797, 2241, 2537
SstI	GAGCTC	1 cut(s)	2577
StuI	AGGCCT	1 cut(s)	1601
StyD4I	CCNGG	5 cut(s)	1476, 1936, 1973, 2053, 2760
StyI	CCWWGG	2 cut(s)	2553, 2733
TaaI	ACNGT	7 cut(s)	85, 1213, 1255, 2127, 2494, 2668, 2854
TaiI	ACGT	2 cut(s)	393, 970
TaqI	TCGA	5 cut(s)	207, 871, 1045, 1852, 3030
TaqII	GACCGA	1 cut(s)	992
TatI	WGTACW	3 cut(s)	1304, 1464, 1666
TauI	GCSGC	1 cut(s)	2590
TfiI	GAWTC	7 cut(s)	8, 191, 369, 516, 826, 1481, 2351
TscAI	CASTG	9 cut(s)	190, 330, 765, 993, 2020, 2077, 2085, 2229, 2538
TseFI	GTSAC	4 cut(s)	1345, 1545, 2224, 2875
Tsp45I	GTSAC	4 cut(s)	1345, 1545, 2224, 2875
TspDTI	ATGAA	9 cut(s)	911, 1241, 1346, 1355, 1383, 1652, 1770, 2460, 2827
TspGWI	ACGGA	3 cut(s)	877, 1079, 1917
TspRI	CASTG	9 cut(s)	190, 330, 765, 993, 2020, 2077, 2085, 2229, 2538
Tth111I	GACNNNGTC	1 cut(s)	2940
Van91I	CCANNNNNTGG	3 cut(s)	36, 1356, 2964
VpaK11BI	GGWCC	3 cut(s)	616, 1858, 2160
XapI	RAATTY	5 cut(s)	333, 1401, 1766, 1833, 2431
XbaI	TCTAGA	1 cut(s)	95
XceI	RCATGY	4 cut(s)	22, 784, 2216, 2376
XhoI	CTCGAG	1 cut(s)	3029
XmiI	GTMKAC	3 cut(s)	87, 768, 2992
XmnI	GAANNNNTTC	1 cut(s)	830
XspI	CTAG	8 cut(s)	96, 339, 942, 1697, 1710, 1797, 2241, 2537
Zsp2I	ATGCAT	2 cut(s)	24, 1814

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.