RchiOBHm_Chr4g0411351

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0016822
Physical Location & Seq
Reverse (-)
35218371 .. 35227316
8946 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1902 bp
ATGTTTGCTGGAAAAGCTGAAGACATAGGAGGAGAAGAAGAAGCAGCAGAGATGAGGATGGAAGAGGGCGCTGAGAAGCCCATAGAGGTGAGGGCTTTAGAGGCGCTTGGGCAGGGGTTTGATTTGAGCAGTGATTTCAGGTTGAAGTTTGCAAAAGGGTTGGATGGGAGTAAGGGGAGGTTGGTGGTTCTGAATGAGGATAGCAAGAGGGACATTGTCATTCCTAGTGGAAGTGGTGGGGGAGTTGTCATTCATGGAGTTCCTCAGGATATTCACTGCGACAAAGGGGATCGAATCCGCTTCAAGTCCGATGTTCTTGAATTCAACCGGATGTCTGAGCTACTTAATCAGAAATCTTCAGTTCAAGGGAAAATTCCTTCAGGATATCTGAATTCCATTTTTGATTTAACTGGAGACTGGCTTCAAGATGCTGCAGAAACAAAATATCTTGCTTTTGATGGTTTCTTCATTTCACTGTACAATTTGCACCTAACAGCATCTCCACTAATTTTGCGAAACAAAGTACAGAAATCTGTTCCATCTCGTTGGGATCCAGCTGCATTGTCTAGGTTCATCCATTCATACGGAACACACATAATAGTTGGGATGGCAGTTGGAGGTCAAGATTTAGTTTGTGTTAGGCAGAAATCTTCCTCACCAGTTCCTCCCGCTGATCTCAGAAGACACTTGGAGGATCTTGGAGATTTTCTATTTTCAGATGGACCTTCTTTACTACGAGGAAAAACAAGAGATGGAAAACAGAAAATTCCGGAGGTGTTCGACCGTATTTTGCAGTCCAACACCATGCATTTATCCACTATTTCCGAAACGTCAAGCAAGGATGGCCTTACAGTCGTTTGCTCAAAAAGAGGAGGTGATGTGTTTCTGAACAGTCACTCCAATTGGCTCCAGACGGTCCTTGCTGAACCAGAAGCAATCCTCTTTAAGTTTGTACCCATTACTTCTCTTCTTACTGGTCTTCCAGGCAGTGGCTATCTCAGTCATGCAATCAACTTGTATCTACGTTATAAGCCTGATGCAGAGGATTTACAACATTTCTTGGAGTTCCAAGTTCCGAGGCAATGGGCGCCAATGTTTTGTGAGCTTCCTCTTTCTCATCAACGGAAAAGGGCTTCTTGTCCTTCCCTGCAATTCAGTTTCATGGGCCCTAAGATCTTTGTTAGCTCTAAGCAGGTTTCAACCAGTCAAAAACCAGTTGTTGGCCTACGCCTGTATTTAGAAGGAAAAAAGTGCAATCGGTTGGCCGTTCATGTGCAGCATCTCTCAAGCCTTCCAAATACTATGGAACTTTCTTTGGCAAACACAAGCACAACAAGTCCAAGCGGGTGGCGAGGTTCTGATGATTGTGAGCTGAGTGGTGAATTCTTAGAGCCGATTAAATGGAAAAGATACTCAAGCATCTGTTCATCTGTAGTTGGACATGACCCCAACTGGTTGAAGGGAGGTCCAAGTGGTGTGTATATTGTAACTGGTGCACAACTACTAAGCAAAGGAAAATGGCCAAAGACGGTACTTCACCTTCGTCTGCATTACACCTTTCTACCCAATTGTGCAATCCGGAAGACTGAATGGGCTGGGTCACCAGAGCCTTCTCACAAATCAACATTCCTTACAAATCTCAGCACCACGTTTACGTTCACTCAAAGGACAGTTACTGATCAGCAAAAGCAGGATCCAGCTGCACTTAATTCAGGTGTATATCCAGAAGGCCCTCCAGTGCTAAATCACTCTTCTAAGCTGCTAAAATTTGTGGACACAGCTGAGGTGGTGCGAGGGCCACATGATGCTCCAGGGCATTGGTTAGTAACAGCTGCAAAGCTAGTAAAAGAGGGACGTAGAATTGGTCTGCATGTAAAGTTTGCCTTGTTAGACTATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

633

Amino Acids

70.08

Weight (kDa)

8.74

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 140 - 340 3.1e-21 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016822)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g12530
malus_domestica MD04G1025600.v1.1
prunus_persica Prupe.1G175000_v2.0.a1
pyrus_communis pycom04g02170
rosa_chinensis RchiOBHm_Chr4g0411351
rosa_laevigata RLG00000008363
rosa_multiflora Rmu_sc0000543.1_g000055
rosa_roxburghii Rroxscaffold_5G00355940
rosa_rugosa Rorug04G0110100
rosa_samantha Rh4AG167300 Rh4CG179000 Rh4DG162100
rosa_wichuraiana Rw4G013830 Rw4G013930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1029
Acc36I ACCTGC 1 cut(s) 1183
AccB1I GGYRCC 1 cut(s) 1087
AccB7I CCANNNNNTGG 2 cut(s) 989, 1220
AccIII TCCGGA 2 cut(s) 769, 1578
AciI CCGC 3 cut(s) 298, 669, 1344
AclWI GGATC 6 cut(s) 297, 545, 558, 702, 1688, 1701
AcoI YGGCCR 2 cut(s) 1263, 1520
AcsI RAATTY 6 cut(s) 320, 372, 391, 765, 1382, 1766
AcuI CTGAAG 3 cut(s) 39, 342, 363
AcyI GRCGYC 1 cut(s) 1088
AfaI GTAC 4 cut(s) 479, 525, 954, 1533
AfiI CCNNNNNNNGG 2 cut(s) 989, 1220
AgsI TTSAA 8 cut(s) 145, 304, 320, 325, 365, 425, 1200, 1459
AjnI CCWGG 2 cut(s) 982, 1810
AloI GAACNNNNNNTCC 2 cut(s) 881, 913
Alw21I GWGCWC 1 cut(s) 1498
Alw26I GTCTC 1 cut(s) 408
Alw44I GTGCAC 1 cut(s) 1494
AlwI GGATC 6 cut(s) 297, 545, 558, 702, 1688, 1701
AlwNI CAGNNNCTG 1 cut(s) 1676
Aor13HI TCCGGA 2 cut(s) 769, 1578
AoxI GGCC 7 cut(s) 844, 1165, 1222, 1263, 1520, 1729, 1796
ApaI GGGCCC 1 cut(s) 1169
ApaLI GTGCAC 1 cut(s) 1494
ApeKI GCWGC 7 cut(s) 44, 431, 557, 1276, 1700, 1759, 1832
ApoI RAATTY 6 cut(s) 320, 372, 391, 765, 1382, 1766
AspLEI GCGC 3 cut(s) 71, 106, 1090
AspS9I GGNCC 7 cut(s) 722, 916, 1165, 1166, 1466, 1730, 1796
AsuHPI GGTGA 6 cut(s) 100, 648, 887, 1391, 1529, 1593
AvaII GGWCC 3 cut(s) 722, 916, 1466
AxyI CCTNAGG 1 cut(s) 264
BaeGI GKGCMC 2 cut(s) 1169, 1498
BalI TGGCCA 1 cut(s) 1522
BamHI GGATCC 2 cut(s) 550, 1693
BanI GGYRCC 1 cut(s) 1087
BanII GRGCYC 1 cut(s) 1169
BarI GAAGNNNNNNTAC 2 cut(s) 1524, 1556
BbsI GAAGAC 4 cut(s) 27, 688, 971, 1589
Bbv12I GWGCWC 1 cut(s) 1498
BbvCI CCTCAGC 1 cut(s) 1782
BbvI GCAGC 7 cut(s) 56, 418, 544, 1288, 1687, 1746, 1819
BccI CCATC 8 cut(s) 52, 158, 452, 547, 601, 713, 746, 836
BceAI ACGGC 1 cut(s) 1250
BciT130I CCWGG 2 cut(s) 984, 1812
BclI TGATCA 1 cut(s) 1678
BcoDI GTCTC 1 cut(s) 408
BfaI CTAG 3 cut(s) 225, 567, 1841
BfmI CTRYAG 2 cut(s) 432, 1431
BfoI RGCGCY 3 cut(s) 72, 107, 1091
BfuAI ACCTGC 1 cut(s) 1183
BglII AGATCT 1 cut(s) 1173
BisI GCNGC 7 cut(s) 45, 432, 558, 1277, 1701, 1760, 1833
BlsI GCNGC 7 cut(s) 46, 433, 559, 1278, 1702, 1761, 1834
Bme1390I CCNGG 2 cut(s) 984, 1812
Bme18I GGWCC 3 cut(s) 722, 916, 1466
BmgT120I GGNCC 7 cut(s) 722, 916, 1165, 1166, 1466, 1730, 1796
BmiI GGNNCC 5 cut(s) 552, 908, 1089, 1167, 1695
BmrFI CCNGG 2 cut(s) 984, 1812
BmsI GCATC 6 cut(s) 418, 506, 1027, 1288, 1428, 1795
BpiI GAAGAC 4 cut(s) 27, 688, 971, 1589
BpmI CTGGAG 4 cut(s) 432, 893, 1719, 1794
Bpu10I CCTNAGC 1 cut(s) 1782
BpuEI CTTGAG 2 cut(s) 1270, 1399
BsaHI GRCGYC 1 cut(s) 1088
BsaJI CCNNGG 2 cut(s) 1076, 1811
BsaWI WCCGGW 3 cut(s) 327, 769, 1578
BsaXI ACNNNNNCTCC 6 cut(s) 484, 514, 864, 881, 894, 911
Bsc4I CCNNNNNNNGG 2 cut(s) 989, 1220
Bse1I ACTGG 9 cut(s) 415, 422, 659, 979, 1203, 1214, 1457, 1495, 1736
Bse21I CCTNAGG 1 cut(s) 264
Bse3DI GCAATG 1 cut(s) 1088
BseAI TCCGGA 2 cut(s) 769, 1578
BseBI CCWGG 2 cut(s) 984, 1812
BseDI CCNNGG 2 cut(s) 1076, 1811
BseGI GGATG 6 cut(s) 63, 169, 336, 573, 612, 847
BseLI CCNNNNNNNGG 2 cut(s) 989, 1220
BseMI GCAATG 1 cut(s) 1088
BseMII CTCAG 8 cut(s) 63, 278, 327, 691, 1012, 1364, 1654, 1773
BseNI ACTGG 9 cut(s) 415, 422, 659, 979, 1203, 1214, 1457, 1495, 1736
BseRI GAGGAG 2 cut(s) 45, 885
BseSI GKGCMC 2 cut(s) 1169, 1498
BseXI GCAGC 7 cut(s) 56, 418, 544, 1288, 1687, 1746, 1819
BseYI CCCAGC 1 cut(s) 1595
BsgI GTGCAG 2 cut(s) 1295, 1686
Bsh1285I CGRYCG 1 cut(s) 784
BshFI GGCC 7 cut(s) 846, 1167, 1224, 1265, 1522, 1731, 1798
BshNI GGYRCC 1 cut(s) 1087
BsiEI CGRYCG 1 cut(s) 784
BsiHKAI GWGCWC 1 cut(s) 1498
BsiSI CCGG 3 cut(s) 328, 770, 1579
BslFI GGGAC 2 cut(s) 224, 1866
BslI CCNNNNNNNGG 2 cut(s) 989, 1220
BsmAI GTCTC 1 cut(s) 408
BsmFI GGGAC 2 cut(s) 224, 1866
BsnI GGCC 7 cut(s) 846, 1167, 1224, 1265, 1522, 1731, 1798
Bsp120I GGGCCC 1 cut(s) 1165
Bsp1286I GDGCHC 2 cut(s) 1169, 1498
Bsp13I TCCGGA 2 cut(s) 769, 1578
Bsp1407I TGTACA 1 cut(s) 477
Bsp143I GATC 7 cut(s) 289, 550, 673, 694, 1173, 1678, 1693
BspACI CCGC 3 cut(s) 298, 669, 1344
BspANI GGCC 7 cut(s) 846, 1167, 1224, 1265, 1522, 1731, 1798
BspCNI CTCAG 8 cut(s) 64, 277, 328, 690, 1011, 1365, 1653, 1774
BspEI TCCGGA 2 cut(s) 769, 1578
BspLI GGNNCC 5 cut(s) 552, 908, 1089, 1167, 1695
BspMAI CTGCAG 1 cut(s) 436
BspMI ACCTGC 1 cut(s) 1183
BspPI GGATC 6 cut(s) 297, 545, 558, 702, 1688, 1701
BspT107I GGYRCC 1 cut(s) 1087
BsrDI GCAATG 1 cut(s) 1088
BsrGI TGTACA 1 cut(s) 477
BsrI ACTGG 9 cut(s) 415, 422, 659, 979, 1203, 1214, 1457, 1495, 1736
BssECI CCNNGG 2 cut(s) 1076, 1811
BssMI GATC 7 cut(s) 289, 550, 673, 694, 1173, 1678, 1693
BssNI GRCGYC 1 cut(s) 1088
Bst2UI CCWGG 2 cut(s) 984, 1812
Bst4CI ACNGT 7 cut(s) 477, 785, 853, 893, 916, 1531, 1672
Bst6I CTCTTC 3 cut(s) 57, 972, 1756
BstACI GRCGYC 1 cut(s) 1088
BstAUI TGTACA 1 cut(s) 477
BstEII GGTNACC 1 cut(s) 1599
BstF5I GGATG 6 cut(s) 63, 169, 336, 573, 612, 847
BstH2I RGCGCY 3 cut(s) 72, 107, 1091
BstHHI GCGC 3 cut(s) 71, 106, 1090
BstKTI GATC 7 cut(s) 292, 553, 676, 697, 1176, 1681, 1696
BstMAI GTCTC 1 cut(s) 408
BstMBI GATC 7 cut(s) 289, 550, 673, 694, 1173, 1678, 1693
BstMCI CGRYCG 1 cut(s) 784
BstMWI GCNNNNNNNGC 4 cut(s) 14, 101, 843, 1087
BstNI CCWGG 2 cut(s) 984, 1812
BstNSI RCATGY 1 cut(s) 1874
BstPI GGTNACC 1 cut(s) 1599
BstSCI CCNGG 2 cut(s) 982, 1810
BstSFI CTRYAG 2 cut(s) 432, 1431
BstSLI GKGCMC 2 cut(s) 1169, 1498
BstV1I GCAGC 7 cut(s) 56, 418, 544, 1288, 1687, 1746, 1819
BstV2I GAAGAC 4 cut(s) 27, 688, 971, 1589
BstX2I RGATCY 4 cut(s) 550, 694, 1173, 1693
BstXI CCANNNNNNTGG 3 cut(s) 546, 1347, 1818
BstYI RGATCY 4 cut(s) 550, 694, 1173, 1693
Bsu36I CCTNAGG 1 cut(s) 264
BsuRI GGCC 7 cut(s) 846, 1167, 1224, 1265, 1522, 1731, 1798
BtsCI GGATG 6 cut(s) 63, 169, 336, 573, 612, 847
BtsI GCAGTG 3 cut(s) 136, 274, 994
BtsIMutI CAGTG 5 cut(s) 136, 274, 473, 994, 1743
BveI ACCTGC 1 cut(s) 1183
CaiI CAGNNNCTG 1 cut(s) 1676
CfoI GCGC 3 cut(s) 71, 106, 1090
Cfr13I GGNCC 7 cut(s) 722, 916, 1165, 1166, 1466, 1730, 1796
Csp6I GTAC 4 cut(s) 478, 524, 953, 1532
CspCI CAANNNNNGTGG 2 cut(s) 492, 527
CviAII CATG 8 cut(s) 254, 805, 1004, 1162, 1271, 1442, 1802, 1871
CviQI GTAC 4 cut(s) 478, 524, 953, 1532
DinI GGCGCC 1 cut(s) 1089
DpnI GATC 7 cut(s) 291, 552, 675, 696, 1175, 1680, 1695
DpnII GATC 7 cut(s) 289, 550, 673, 694, 1173, 1678, 1693
EaeI YGGCCR 2 cut(s) 1263, 1520
Eam1104I CTCTTC 3 cut(s) 57, 972, 1756
EarI CTCTTC 3 cut(s) 57, 972, 1756
Eco24I GRGCYC 1 cut(s) 1169
Eco32I GATATC 1 cut(s) 386
Eco47I GGWCC 3 cut(s) 722, 916, 1466
Eco57I CTGAAG 3 cut(s) 39, 342, 363
Eco81I CCTNAGG 1 cut(s) 264
Eco91I GGTNACC 1 cut(s) 1599
EcoO109I RGGNCCY 2 cut(s) 1166, 1730
EcoO65I GGTNACC 1 cut(s) 1599
EcoRI GAATTC 3 cut(s) 320, 391, 1382
EcoRII CCWGG 2 cut(s) 982, 1810
EcoRV GATATC 1 cut(s) 386
EcoT22I ATGCAT 1 cut(s) 810
EcoT38I GRGCYC 1 cut(s) 1169
EgeI GGCGCC 1 cut(s) 1089
EheI GGCGCC 1 cut(s) 1089
FaeI CATG 8 cut(s) 257, 808, 1007, 1165, 1274, 1445, 1805, 1874
FalI AAGNNNNNCTT 4 cut(s) 1120, 1152, 1868, 1900
FaqI GGGAC 2 cut(s) 224, 1866
FatI CATG 8 cut(s) 253, 804, 1003, 1161, 1270, 1441, 1801, 1870
FauI CCCGC 2 cut(s) 676, 1337
FbaI TGATCA 1 cut(s) 1678
Fnu4HI GCNGC 7 cut(s) 45, 432, 558, 1277, 1701, 1760, 1833
FokI GGATG 6 cut(s) 70, 176, 343, 560, 619, 854
FriOI GRGCYC 1 cut(s) 1169
Fsp4HI GCNGC 7 cut(s) 45, 432, 558, 1277, 1701, 1760, 1833
FspBI CTAG 3 cut(s) 225, 567, 1841
GlaI GCGC 3 cut(s) 70, 105, 1089
GluI GCNGC 7 cut(s) 45, 432, 558, 1277, 1701, 1760, 1833
GsaI CCCAGC 1 cut(s) 1599
GsuI CTGGAG 4 cut(s) 432, 893, 1719, 1794
HaeII RGCGCY 3 cut(s) 72, 107, 1091
HaeIII GGCC 7 cut(s) 846, 1167, 1224, 1265, 1522, 1731, 1798
HapII CCGG 3 cut(s) 328, 770, 1579
HhaI GCGC 3 cut(s) 71, 106, 1090
Hin1I GRCGYC 1 cut(s) 1088
Hin1II CATG 8 cut(s) 257, 808, 1007, 1165, 1274, 1445, 1805, 1874
Hin6I GCGC 3 cut(s) 69, 104, 1088
HinP1I GCGC 3 cut(s) 69, 104, 1088
HinfI GANTC 1 cut(s) 294
HpaII CCGG 3 cut(s) 328, 770, 1579
HphI GGTGA 6 cut(s) 100, 648, 887, 1391, 1529, 1593
Hpy166II GTNNAC 4 cut(s) 1496, 1653, 1659, 1774
Hpy188III TCNNGA 9 cut(s) 266, 317, 381, 425, 623, 770, 910, 1579, 1724
Hpy8I GTNNAC 4 cut(s) 1496, 1653, 1659, 1774
HpyAV CCTTC 9 cut(s) 387, 735, 1152, 1235, 1301, 1453, 1550, 1620, 1721
HpyCH4III ACNGT 7 cut(s) 477, 785, 853, 893, 916, 1531, 1672
HpyCH4IV ACGT 5 cut(s) 830, 1024, 1649, 1655, 1855
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 101, 843, 1087
HpySE526I ACGT 5 cut(s) 830, 1024, 1649, 1655, 1855
Hsp92I GRCGYC 1 cut(s) 1088
Hsp92II CATG 8 cut(s) 257, 808, 1007, 1165, 1274, 1445, 1805, 1874
HspAI GCGC 3 cut(s) 69, 104, 1088
KasI GGCGCC 1 cut(s) 1087
Kpn2I TCCGGA 2 cut(s) 769, 1578
Ksp22I TGATCA 1 cut(s) 1678
Kzo9I GATC 7 cut(s) 289, 550, 673, 694, 1173, 1678, 1693
LmnI GCTCC 2 cut(s) 912, 1813
Lsp1109I GCAGC 7 cut(s) 56, 418, 544, 1288, 1687, 1746, 1819
LweI GCATC 6 cut(s) 418, 506, 1027, 1288, 1428, 1795
MaeI CTAG 3 cut(s) 225, 567, 1841
MaeII ACGT 5 cut(s) 830, 1024, 1649, 1655, 1855
MaeIII GTNAC 5 cut(s) 893, 1486, 1599, 1672, 1825
MalI GATC 7 cut(s) 291, 552, 675, 696, 1175, 1680, 1695
MboI GATC 7 cut(s) 289, 550, 673, 694, 1173, 1678, 1693
MfeI CAATTG 2 cut(s) 901, 1567
MflI RGATCY 4 cut(s) 550, 694, 1173, 1693
MhlI GDGCHC 2 cut(s) 1169, 1498
MlsI TGGCCA 1 cut(s) 1522
MluNI TGGCCA 1 cut(s) 1522
Mly113I GGCGCC 1 cut(s) 1088
MmeI TCCRAC 4 cut(s) 141, 595, 822, 1417
Mox20I TGGCCA 1 cut(s) 1522
Mph1103I ATGCAT 1 cut(s) 810
MroI TCCGGA 2 cut(s) 769, 1578
MscI TGGCCA 1 cut(s) 1522
MseI TTAA 5 cut(s) 345, 407, 945, 1398, 1707
MslI CAYNNNNRTG 1 cut(s) 86
Msp20I TGGCCA 1 cut(s) 1522
MspA1I CMGCKG 5 cut(s) 557, 671, 1700, 1781, 1832
MspI CCGG 3 cut(s) 328, 770, 1579
MspR9I CCNGG 2 cut(s) 984, 1812
MunI CAATTG 2 cut(s) 901, 1567
MvaI CCWGG 2 cut(s) 984, 1812
MwoI GCNNNNNNNGC 4 cut(s) 14, 101, 843, 1087
NarI GGCGCC 1 cut(s) 1088
NdeII GATC 7 cut(s) 289, 550, 673, 694, 1173, 1678, 1693
NlaIII CATG 8 cut(s) 257, 808, 1007, 1165, 1274, 1445, 1805, 1874
NlaIV GGNNCC 5 cut(s) 552, 908, 1089, 1167, 1695
NmuCI GTSAC 2 cut(s) 893, 1599
NsiI ATGCAT 1 cut(s) 810
NspI RCATGY 1 cut(s) 1874
PfeI GAWTC 1 cut(s) 294
PflFI GACNNNGTC 1 cut(s) 215
PflMI CCANNNNNTGG 2 cut(s) 989, 1220
PkrI GCNGC 7 cut(s) 46, 433, 559, 1278, 1702, 1761, 1834
PluTI GGCGCC 1 cut(s) 1091
PsiI TTATAA 1 cut(s) 1029
Psp6I CCWGG 2 cut(s) 982, 1810
PspEI GGTNACC 1 cut(s) 1599
PspFI CCCAGC 1 cut(s) 1595
PspGI CCWGG 2 cut(s) 982, 1810
PspN4I GGNNCC 5 cut(s) 552, 908, 1089, 1167, 1695
PspOMI GGGCCC 1 cut(s) 1165
PspPI GGNCC 7 cut(s) 722, 916, 1165, 1166, 1466, 1730, 1796
PstI CTGCAG 1 cut(s) 436
PstNI CAGNNNCTG 1 cut(s) 1676
PsuI RGATCY 4 cut(s) 550, 694, 1173, 1693
PsyI GACNNNGTC 1 cut(s) 215
PvuII CAGCTG 4 cut(s) 557, 1700, 1781, 1832
RsaI GTAC 4 cut(s) 479, 525, 954, 1533
RsaNI GTAC 4 cut(s) 478, 524, 953, 1532
RseI CAYNNNNRTG 1 cut(s) 86
SaqAI TTAA 5 cut(s) 345, 407, 945, 1398, 1707
SatI GCNGC 7 cut(s) 45, 432, 558, 1277, 1701, 1760, 1833
Sau3AI GATC 7 cut(s) 289, 550, 673, 694, 1173, 1678, 1693
Sau96I GGNCC 7 cut(s) 722, 916, 1165, 1166, 1466, 1730, 1796
ScrFI CCNGG 2 cut(s) 984, 1812
SduI GDGCHC 2 cut(s) 1169, 1498
SfaNI GCATC 6 cut(s) 418, 506, 1027, 1288, 1428, 1795
SfcI CTRYAG 2 cut(s) 432, 1431
SfoI GGCGCC 1 cut(s) 1089
SinI GGWCC 3 cut(s) 722, 916, 1466
SmiMI CAYNNNNRTG 1 cut(s) 86
SmlI CTYRAG 2 cut(s) 1285, 1414
SmoI CTYRAG 2 cut(s) 1285, 1414
SsiI CCGC 3 cut(s) 298, 669, 1344
SspDI GGCGCC 1 cut(s) 1087
SspMI CTAG 3 cut(s) 225, 567, 1841
StyD4I CCNGG 2 cut(s) 982, 1810
TaaI ACNGT 7 cut(s) 477, 785, 853, 893, 916, 1531, 1672
TaiI ACGT 5 cut(s) 833, 1027, 1652, 1658, 1858
TaqI TCGA 2 cut(s) 292, 780
TatI WGTACW 2 cut(s) 477, 523
TfiI GAWTC 1 cut(s) 294
Tru1I TTAA 5 cut(s) 345, 407, 945, 1398, 1707
Tru9I TTAA 5 cut(s) 345, 407, 945, 1398, 1707
TscAI CASTG 5 cut(s) 136, 281, 480, 994, 1743
TseFI GTSAC 2 cut(s) 893, 1599
TseI GCWGC 7 cut(s) 44, 431, 557, 1276, 1700, 1759, 1832
Tsp45I GTSAC 2 cut(s) 893, 1599
TspDTI ATGAA 7 cut(s) 242, 457, 562, 570, 1150, 1259, 1416
TspGWI ACGGA 2 cut(s) 600, 1138
TspRI CASTG 5 cut(s) 136, 281, 480, 994, 1743
Tth111I GACNNNGTC 1 cut(s) 215
Van91I CCANNNNNTGG 2 cut(s) 989, 1220
VneI GTGCAC 1 cut(s) 1494
VpaK11BI GGWCC 3 cut(s) 722, 916, 1466
XapI RAATTY 6 cut(s) 320, 372, 391, 765, 1382, 1766
XceI RCATGY 1 cut(s) 1874
XspI CTAG 3 cut(s) 225, 567, 1841
Zsp2I ATGCAT 1 cut(s) 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.