RchiOBHm_Chr4g0439651

GMC oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0022716
Physical Location & Seq
Forward (+)
62058806 .. 62059435
630 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 630 bp
ATGCAAGACAATCCGGCTATTGCAATCTTAGTTGACTCCAAGCCACAAAATCGAACACCAGAACCTGCCAAAGTTGCTGGTATAGCAGATGACTTCAGAATCATAATTGAGGCAGGAATTGCACCTGTTAGCTTCAATGCAACAATAGTTCGCATTACTGGCAAAATTGCCTTCCCAGAATCCGTAGGAAAGCTTGAACTCTACAGCACTGACCCAAGAGCAAACCCATCAGTGACATTCAACTACCTATCAAGCGAGAAAGACTTGGCACAATGTGTGAAGCTGACCCAGCTGCTTGAGCTAGCTTTAAGGTCCAAATCAATTGCTTCCTTCCTGGGTTCAAATGAACACCGAAACAAGCCGATGCCTACTAAAGACGAGCTCCAGAAACTGTGCAAGACGACTGTCAGAACCTTCTACCACTACCATGGTGGTTGCACTATGGGGTCAGTGGTCGACAAGAATTATAGGGTTTATGGTGTTAAAGGCTTGAGAGTGATTGATGGGTCAACCTTGCTGGAATCACCAGGCACAAACCCAATGGCCACTCTGCTAATGCTTGGAAGATACCAAGGGCTCAAAATTCTTAAACACAGAAAAGGTGCTTCCTTTAACAGTCCCAGCACCTGA

Protein Analysis

209

Amino Acids

22.9

Weight (kDa)

9.51

Isoelectric Point (pI)

33.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_C PF05199 59 - 188 5.2e-30 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0022716)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0439651
rosa_multiflora Rmu_co8019486.1_g000001 Rmu_sc0007322.1_g000003
rosa_samantha Rh5DG045200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 73
AccI GTMKAC 1 cut(s) 456
AcoI YGGCCR 1 cut(s) 543
AcsI RAATTY 1 cut(s) 582
AcuI CTGAAG 1 cut(s) 79
AdeI CACNNNGTG 1 cut(s) 275
AgsI TTSAA 4 cut(s) 136, 197, 241, 342
AjnI CCWGG 2 cut(s) 333, 526
AluBI AGCT 7 cut(s) 132, 193, 283, 292, 301, 305, 382
AluI AGCT 7 cut(s) 132, 193, 283, 292, 301, 305, 382
Alw21I GWGCWC 1 cut(s) 384
AlwNI CAGNNNCTG 3 cut(s) 65, 391, 627
AoxI GGCC 1 cut(s) 543
ApeKI GCWGC 1 cut(s) 292
ApoI RAATTY 1 cut(s) 582
AspS9I GGNCC 1 cut(s) 312
AsuHPI GGTGA 1 cut(s) 516
AsuNHI GCTAGC 1 cut(s) 301
AvaII GGWCC 1 cut(s) 312
BalI TGGCCA 1 cut(s) 545
BanII GRGCYC 2 cut(s) 384, 579
Bbv12I GWGCWC 1 cut(s) 384
BbvI GCAGC 1 cut(s) 279
BccI CCATC 2 cut(s) 235, 497
BciT130I CCWGG 2 cut(s) 335, 528
BfaI CTAG 1 cut(s) 302
BfmI CTRYAG 1 cut(s) 202
BfuAI ACCTGC 1 cut(s) 73
BisI GCNGC 1 cut(s) 293
BlsI GCNGC 1 cut(s) 294
Bme1390I CCNGG 2 cut(s) 335, 528
Bme18I GGWCC 1 cut(s) 312
BmgT120I GGNCC 1 cut(s) 312
BmrFI CCNGG 2 cut(s) 335, 528
BmsI GCATC 1 cut(s) 354
BmtI GCTAGC 1 cut(s) 305
BoxI GACNNNNGTC 1 cut(s) 404
BpmI CTGGAG 1 cut(s) 368
BpuEI CTTGAG 2 cut(s) 317, 511
BsaJI CCNNGG 3 cut(s) 334, 427, 571
Bse1I ACTGG 1 cut(s) 163
BseBI CCWGG 2 cut(s) 335, 528
BseDI CCNNGG 3 cut(s) 334, 427, 571
BseNI ACTGG 1 cut(s) 163
BseXI GCAGC 1 cut(s) 279
BseYI CCCAGC 2 cut(s) 288, 620
BshFI GGCC 1 cut(s) 545
BsiHKAI GWGCWC 1 cut(s) 384
BsiSI CCGG 1 cut(s) 14
BslFI GGGAC 1 cut(s) 603
BsmFI GGGAC 1 cut(s) 603
BsnI GGCC 1 cut(s) 545
Bsp1286I GDGCHC 2 cut(s) 384, 579
Bsp19I CCATGG 1 cut(s) 427
BspANI GGCC 1 cut(s) 545
BspMI ACCTGC 1 cut(s) 73
BspOI GCTAGC 1 cut(s) 305
BsrI ACTGG 1 cut(s) 163
BssECI CCNNGG 3 cut(s) 334, 427, 571
BssT1I CCWWGG 2 cut(s) 427, 571
Bst2UI CCWGG 2 cut(s) 335, 528
Bst4CI ACNGT 3 cut(s) 393, 406, 617
BstAPI GCANNNNNTGC 1 cut(s) 119
BstC8I GCNNGC 1 cut(s) 303
BstDEI CTNAG 1 cut(s) 28
BstDSI CCRYGG 1 cut(s) 427
BstMWI GCNNNNNNNGC 6 cut(s) 74, 83, 119, 159, 289, 298
BstNI CCWGG 2 cut(s) 335, 528
BstPAI GACNNNNGTC 1 cut(s) 404
BstSCI CCNGG 2 cut(s) 333, 526
BstSFI CTRYAG 1 cut(s) 202
BstV1I GCAGC 1 cut(s) 279
BstXI CCANNNNNNTGG 1 cut(s) 428
BsuRI GGCC 1 cut(s) 545
BtgI CCRYGG 1 cut(s) 427
BtsIMutI CAGTG 3 cut(s) 207, 237, 456
BveI ACCTGC 1 cut(s) 73
Cac8I GCNNGC 1 cut(s) 303
CaiI CAGNNNCTG 3 cut(s) 65, 391, 627
Cfr13I GGNCC 1 cut(s) 312
CviAII CATG 1 cut(s) 428
DdeI CTNAG 1 cut(s) 28
DraIII CACNNNGTG 1 cut(s) 275
EaeI YGGCCR 1 cut(s) 543
Ecl136II GAGCTC 1 cut(s) 382
Eco130I CCWWGG 2 cut(s) 427, 571
Eco24I GRGCYC 2 cut(s) 384, 579
Eco47I GGWCC 1 cut(s) 312
Eco53kI GAGCTC 1 cut(s) 382
Eco57I CTGAAG 1 cut(s) 79
EcoICRI GAGCTC 1 cut(s) 382
EcoRII CCWGG 2 cut(s) 333, 526
EcoT14I CCWWGG 2 cut(s) 427, 571
EcoT38I GRGCYC 2 cut(s) 384, 579
ErhI CCWWGG 2 cut(s) 427, 571
FaeI CATG 1 cut(s) 431
FaiI YATR 6 cut(s) 83, 104, 429, 443, 468, 477
FaqI GGGAC 1 cut(s) 603
FatI CATG 1 cut(s) 427
FblI GTMKAC 1 cut(s) 456
Fnu4HI GCNGC 1 cut(s) 293
FriOI GRGCYC 2 cut(s) 384, 579
Fsp4HI GCNGC 1 cut(s) 293
FspBI CTAG 1 cut(s) 302
GluI GCNGC 1 cut(s) 293
GsaI CCCAGC 2 cut(s) 292, 624
GsuI CTGGAG 1 cut(s) 368
HaeIII GGCC 1 cut(s) 545
HapII CCGG 1 cut(s) 14
Hin1II CATG 1 cut(s) 431
HincII GTYRAC 3 cut(s) 34, 457, 510
HindII GTYRAC 3 cut(s) 34, 457, 510
HindIII AAGCTT 1 cut(s) 191
HinfI GANTC 4 cut(s) 35, 99, 179, 521
HpaII CCGG 1 cut(s) 14
HphI GGTGA 1 cut(s) 516
Hpy166II GTNNAC 3 cut(s) 34, 457, 510
Hpy188I TCNGA 2 cut(s) 98, 410
Hpy188III TCNNGA 1 cut(s) 385
Hpy8I GTNNAC 3 cut(s) 34, 457, 510
HpyAV CCTTC 3 cut(s) 181, 340, 424
HpyCH4III ACNGT 3 cut(s) 393, 406, 617
HpyCH4V TGCA 6 cut(s) 4, 23, 122, 140, 396, 438
HpyF10VI GCNNNNNNNGC 6 cut(s) 74, 83, 119, 159, 289, 298
HpyF3I CTNAG 1 cut(s) 28
Hsp92II CATG 1 cut(s) 431
LmnI GCTCC 1 cut(s) 387
Lsp1109I GCAGC 1 cut(s) 279
LweI GCATC 1 cut(s) 354
MaeI CTAG 1 cut(s) 302
MaeIII GTNAC 1 cut(s) 232
MboII GAAGA 1 cut(s) 576
MfeI CAATTG 1 cut(s) 321
MhlI GDGCHC 2 cut(s) 384, 579
MlsI TGGCCA 1 cut(s) 545
MluCI AATT 6 cut(s) 105, 117, 165, 321, 463, 582
MluNI TGGCCA 1 cut(s) 545
MlyI GAGTC 1 cut(s) 29
MnlI CCTC 1 cut(s) 103
Mox20I TGGCCA 1 cut(s) 545
MscI TGGCCA 1 cut(s) 545
MseI TTAA 4 cut(s) 308, 483, 588, 612
MslI CAYNNNNRTG 1 cut(s) 426
Msp20I TGGCCA 1 cut(s) 545
MspA1I CMGCKG 1 cut(s) 292
MspI CCGG 1 cut(s) 14
MspR9I CCNGG 2 cut(s) 335, 528
MunI CAATTG 1 cut(s) 321
MvaI CCWGG 2 cut(s) 335, 528
MwoI GCNNNNNNNGC 6 cut(s) 74, 83, 119, 159, 289, 298
NcoI CCATGG 1 cut(s) 427
NheI GCTAGC 1 cut(s) 301
NlaIII CATG 1 cut(s) 431
NmuCI GTSAC 1 cut(s) 232
PfeI GAWTC 3 cut(s) 99, 179, 521
PkrI GCNGC 1 cut(s) 294
PleI GAGTC 1 cut(s) 29
PpsI GAGTC 1 cut(s) 29
PshAI GACNNNNGTC 1 cut(s) 404
Psp124BI GAGCTC 1 cut(s) 384
Psp6I CCWGG 2 cut(s) 333, 526
PspFI CCCAGC 2 cut(s) 288, 620
PspGI CCWGG 2 cut(s) 333, 526
PspPI GGNCC 1 cut(s) 312
PstNI CAGNNNCTG 3 cut(s) 65, 391, 627
PvuII CAGCTG 1 cut(s) 292
RseI CAYNNNNRTG 1 cut(s) 426
SacI GAGCTC 1 cut(s) 384
SalI GTCGAC 1 cut(s) 455
SaqAI TTAA 4 cut(s) 308, 483, 588, 612
SatI GCNGC 1 cut(s) 293
Sau96I GGNCC 1 cut(s) 312
SchI GAGTC 1 cut(s) 29
ScrFI CCNGG 2 cut(s) 335, 528
SduI GDGCHC 2 cut(s) 384, 579
SfaNI GCATC 1 cut(s) 354
SfcI CTRYAG 1 cut(s) 202
SinI GGWCC 1 cut(s) 312
SmiMI CAYNNNNRTG 1 cut(s) 426
SmlI CTYRAG 2 cut(s) 296, 490
SmoI CTYRAG 2 cut(s) 296, 490
Sse9I AATT 6 cut(s) 105, 117, 165, 321, 463, 582
SspMI CTAG 1 cut(s) 302
SstI GAGCTC 1 cut(s) 384
StyD4I CCNGG 2 cut(s) 333, 526
StyI CCWWGG 2 cut(s) 427, 571
TaaI ACNGT 3 cut(s) 393, 406, 617
TaqI TCGA 2 cut(s) 52, 456
TasI AATT 6 cut(s) 105, 117, 165, 321, 463, 582
TfiI GAWTC 3 cut(s) 99, 179, 521
Tru1I TTAA 4 cut(s) 308, 483, 588, 612
Tru9I TTAA 4 cut(s) 308, 483, 588, 612
TscAI CASTG 3 cut(s) 214, 237, 456
TseFI GTSAC 1 cut(s) 232
TseI GCWGC 1 cut(s) 292
Tsp45I GTSAC 1 cut(s) 232
TspDTI ATGAA 1 cut(s) 360
TspGWI ACGGA 1 cut(s) 172
TspRI CASTG 3 cut(s) 214, 237, 456
VpaK11BI GGWCC 1 cut(s) 312
XapI RAATTY 1 cut(s) 582
XcmI CCANNNNNNNNNTGG 1 cut(s) 428
XmiI GTMKAC 1 cut(s) 456
XspI CTAG 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.