Rh5DG045200

GMC oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0022716
Physical Location & Seq
Reverse (-)
3743564 .. 3744196
633 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG045200.1

Sequence Viewer

Length: 633 bp
ATGCAAGACAATACTGCTATTCAAGTCTTTGTTAACTACAACCAGCAAAATCCAACACCAGACCCTGCCAAAGTTGCTGGTATAGCAGATGACTTCAAAATCATAATCGAGGCAGCAATCCTACCTATTAGCTTGAATGGAACATTTTTTAGCTATTTTATTGGCAAACTTGCCTTCCCAGAATCCGTAGGAAAGCTTGAACTCAACAGCACTGACCCCAGAGCAAACTCATCAGTGACGTTCAACTACCTATCAAGCGAGAAAGACTTGGCAGAATGTGTGAAGATGACCGAGTTGCTTGAGCTAGCTTTAAGGTCCAAATCAATTGCTTCCTTAGTGGGTTCGAATGAATACCGAAACAAGCCGATGCCTACTAAAGACGAGCTCCAGAAACTCTGCAAGACGACTATCAGTACCATCTGGCACTACCATGGTGGTTGCACTGTGGGGTCAGTGGTTGAAAAGAATTATAGGGTTTATGGTGTTAAAGGCTTGAGAGTGATCGATGGGTCAACCTTCCTAGAATCACCAGGCACAAACCCAATGGCCACTGTGCTGATGCTTGGAAGATACCAAGGACTCAAAATTCTTCAAGAAAGAAAAGATGCTTCCTTTATCAATCCCATAACCTGA

Protein Analysis

210

Amino Acids

23.19

Weight (kDa)

7.65

Isoelectric Point (pI)

33.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_C PF05199 60 - 189 2.5e-28 GMC oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0022716)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr4g0439651
rosa_multiflora Rmu_co8019486.1_g000001 Rmu_sc0007322.1_g000003
rosa_samantha Rh5DG045200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 546
AcsI RAATTY 1 cut(s) 585
AfaI GTAC 1 cut(s) 415
AgsI TTSAA 7 cut(s) 23, 97, 136, 200, 244, 461, 593
AjnI CCWGG 1 cut(s) 529
AluBI AGCT 6 cut(s) 132, 153, 196, 304, 308, 385
AluI AGCT 6 cut(s) 132, 153, 196, 304, 308, 385
Alw21I GWGCWC 1 cut(s) 387
AlwNI CAGNNNCTG 1 cut(s) 65
AoxI GGCC 1 cut(s) 546
ApeKI GCWGC 1 cut(s) 113
ApoI RAATTY 1 cut(s) 585
AspS9I GGNCC 1 cut(s) 315
AsuHPI GGTGA 1 cut(s) 519
AsuII TTCGAA 1 cut(s) 344
AsuNHI GCTAGC 1 cut(s) 304
AvaII GGWCC 1 cut(s) 315
BalI TGGCCA 1 cut(s) 548
BanII GRGCYC 1 cut(s) 387
Bbv12I GWGCWC 1 cut(s) 387
BbvI GCAGC 1 cut(s) 125
BccI CCATC 2 cut(s) 425, 500
BciT130I CCWGG 1 cut(s) 531
BfaI CTAG 2 cut(s) 305, 521
BisI GCNGC 1 cut(s) 114
BlsI GCNGC 1 cut(s) 115
Bme1390I CCNGG 1 cut(s) 531
Bme18I GGWCC 1 cut(s) 315
BmgT120I GGNCC 1 cut(s) 315
BmrFI CCNGG 1 cut(s) 531
BmsI GCATC 3 cut(s) 357, 549, 595
BmtI GCTAGC 1 cut(s) 308
BpmI CTGGAG 1 cut(s) 371
Bpu14I TTCGAA 1 cut(s) 344
BpuEI CTTGAG 2 cut(s) 320, 514
Bsa29I ATCGAT 1 cut(s) 504
BsaJI CCNNGG 2 cut(s) 430, 574
BseBI CCWGG 1 cut(s) 531
BseCI ATCGAT 1 cut(s) 504
BseDI CCNNGG 2 cut(s) 430, 574
BseXI GCAGC 1 cut(s) 125
BshFI GGCC 1 cut(s) 548
BshVI ATCGAT 1 cut(s) 504
BsiHKAI GWGCWC 1 cut(s) 387
BsnI GGCC 1 cut(s) 548
Bsp119I TTCGAA 1 cut(s) 344
Bsp1286I GDGCHC 1 cut(s) 387
Bsp143I GATC 1 cut(s) 501
Bsp19I CCATGG 1 cut(s) 430
BspANI GGCC 1 cut(s) 548
BspDI ATCGAT 1 cut(s) 504
BspOI GCTAGC 1 cut(s) 308
BspT104I TTCGAA 1 cut(s) 344
BssECI CCNNGG 2 cut(s) 430, 574
BssMI GATC 1 cut(s) 501
BssT1I CCWWGG 2 cut(s) 430, 574
Bst2UI CCWGG 1 cut(s) 531
Bst4CI ACNGT 2 cut(s) 445, 553
BstBI TTCGAA 1 cut(s) 344
BstC8I GCNNGC 1 cut(s) 306
BstDEI CTNAG 1 cut(s) 334
BstDSI CCRYGG 1 cut(s) 430
BstKTI GATC 1 cut(s) 504
BstMBI GATC 1 cut(s) 501
BstMWI GCNNNNNNNGC 2 cut(s) 74, 83
BstNI CCWGG 1 cut(s) 531
BstSCI CCNGG 1 cut(s) 529
BstV1I GCAGC 1 cut(s) 125
Bsu15I ATCGAT 1 cut(s) 504
BsuRI GGCC 1 cut(s) 548
BsuTUI ATCGAT 1 cut(s) 504
BtgI CCRYGG 1 cut(s) 430
BtsIMutI CAGTG 5 cut(s) 210, 240, 441, 459, 549
Cac8I GCNNGC 1 cut(s) 306
CaiI CAGNNNCTG 1 cut(s) 65
Cfr13I GGNCC 1 cut(s) 315
ClaI ATCGAT 1 cut(s) 504
Csp6I GTAC 1 cut(s) 414
CviAII CATG 1 cut(s) 431
CviJI RGCY 9 cut(s) 132, 153, 196, 304, 308, 364, 385, 492, 548
CviKI_1 RGCY 9 cut(s) 132, 153, 196, 304, 308, 364, 385, 492, 548
CviQI GTAC 1 cut(s) 414
DdeI CTNAG 1 cut(s) 334
DpnI GATC 1 cut(s) 503
DpnII GATC 1 cut(s) 501
EaeI YGGCCR 1 cut(s) 546
Ecl136II GAGCTC 1 cut(s) 385
Eco130I CCWWGG 2 cut(s) 430, 574
Eco24I GRGCYC 1 cut(s) 387
Eco47I GGWCC 1 cut(s) 315
Eco53kI GAGCTC 1 cut(s) 385
EcoICRI GAGCTC 1 cut(s) 385
EcoRII CCWGG 1 cut(s) 529
EcoT14I CCWWGG 2 cut(s) 430, 574
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 2 cut(s) 430, 574
FaeI CATG 1 cut(s) 434
FaiI YATR 6 cut(s) 83, 104, 432, 471, 480, 626
FatI CATG 1 cut(s) 430
Fnu4HI GCNGC 1 cut(s) 114
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 1 cut(s) 114
FspBI CTAG 2 cut(s) 305, 521
GluI GCNGC 1 cut(s) 114
GsuI CTGGAG 1 cut(s) 371
HaeIII GGCC 1 cut(s) 548
Hin1II CATG 1 cut(s) 434
HincII GTYRAC 2 cut(s) 34, 513
HindII GTYRAC 2 cut(s) 34, 513
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 3 cut(s) 182, 524, 579
HpaI GTTAAC 1 cut(s) 34
HphI GGTGA 1 cut(s) 519
Hpy166II GTNNAC 2 cut(s) 34, 513
Hpy188III TCNNGA 2 cut(s) 388, 593
Hpy8I GTNNAC 2 cut(s) 34, 513
HpyAV CCTTC 2 cut(s) 184, 526
HpyCH4III ACNGT 2 cut(s) 445, 553
HpyCH4IV ACGT 1 cut(s) 239
HpyCH4V TGCA 3 cut(s) 4, 399, 441
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 83
HpyF3I CTNAG 1 cut(s) 334
HpySE526I ACGT 1 cut(s) 239
Hsp92II CATG 1 cut(s) 434
KspAI GTTAAC 1 cut(s) 34
Kzo9I GATC 1 cut(s) 501
LmnI GCTCC 1 cut(s) 390
Lsp1109I GCAGC 1 cut(s) 125
LweI GCATC 3 cut(s) 357, 549, 595
MaeI CTAG 2 cut(s) 305, 521
MaeII ACGT 1 cut(s) 239
MaeIII GTNAC 1 cut(s) 235
MalI GATC 1 cut(s) 503
MboI GATC 1 cut(s) 501
MboII GAAGA 3 cut(s) 295, 579, 581
MfeI CAATTG 1 cut(s) 324
MhlI GDGCHC 1 cut(s) 387
MlsI TGGCCA 1 cut(s) 548
MluCI AATT 3 cut(s) 324, 466, 585
MluNI TGGCCA 1 cut(s) 548
MlyI GAGTC 1 cut(s) 573
MmeI TCCRAC 1 cut(s) 77
MnlI CCTC 1 cut(s) 103
Mox20I TGGCCA 1 cut(s) 548
MscI TGGCCA 1 cut(s) 548
MseI TTAA 3 cut(s) 33, 311, 486
MslI CAYNNNNRTG 1 cut(s) 429
Msp20I TGGCCA 1 cut(s) 548
MspR9I CCNGG 1 cut(s) 531
MunI CAATTG 1 cut(s) 324
MvaI CCWGG 1 cut(s) 531
MwoI GCNNNNNNNGC 2 cut(s) 74, 83
NcoI CCATGG 1 cut(s) 430
NdeII GATC 1 cut(s) 501
NheI GCTAGC 1 cut(s) 304
NlaIII CATG 1 cut(s) 434
NmuCI GTSAC 1 cut(s) 235
NspV TTCGAA 1 cut(s) 344
PfeI GAWTC 2 cut(s) 182, 524
PkrI GCNGC 1 cut(s) 115
PleI GAGTC 1 cut(s) 573
PpsI GAGTC 1 cut(s) 573
Psp124BI GAGCTC 1 cut(s) 387
Psp6I CCWGG 1 cut(s) 529
PspGI CCWGG 1 cut(s) 529
PspPI GGNCC 1 cut(s) 315
PstNI CAGNNNCTG 1 cut(s) 65
RsaI GTAC 1 cut(s) 415
RsaNI GTAC 1 cut(s) 414
RseI CAYNNNNRTG 1 cut(s) 429
SacI GAGCTC 1 cut(s) 387
SaqAI TTAA 3 cut(s) 33, 311, 486
SatI GCNGC 1 cut(s) 114
Sau3AI GATC 1 cut(s) 501
Sau96I GGNCC 1 cut(s) 315
SchI GAGTC 1 cut(s) 573
ScrFI CCNGG 1 cut(s) 531
SduI GDGCHC 1 cut(s) 387
SfaNI GCATC 3 cut(s) 357, 549, 595
SfuI TTCGAA 1 cut(s) 344
SinI GGWCC 1 cut(s) 315
SmiMI CAYNNNNRTG 1 cut(s) 429
SmlI CTYRAG 2 cut(s) 299, 493
SmoI CTYRAG 2 cut(s) 299, 493
Sse9I AATT 3 cut(s) 324, 466, 585
SspMI CTAG 2 cut(s) 305, 521
SstI GAGCTC 1 cut(s) 387
StyD4I CCNGG 1 cut(s) 529
StyI CCWWGG 2 cut(s) 430, 574
TaaI ACNGT 2 cut(s) 445, 553
TaiI ACGT 1 cut(s) 242
TaqI TCGA 3 cut(s) 108, 344, 504
TaqII GACCGA 1 cut(s) 305
TasI AATT 3 cut(s) 324, 466, 585
TfiI GAWTC 2 cut(s) 182, 524
Tru1I TTAA 3 cut(s) 33, 311, 486
Tru9I TTAA 3 cut(s) 33, 311, 486
TscAI CASTG 5 cut(s) 217, 240, 448, 459, 556
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 1 cut(s) 113
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 1 cut(s) 363
TspGWI ACGGA 1 cut(s) 175
TspRI CASTG 5 cut(s) 217, 240, 448, 459, 556
VpaK11BI GGWCC 1 cut(s) 315
XapI RAATTY 1 cut(s) 585
XspI CTAG 2 cut(s) 305, 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.