RchiOBHm_Chr3g0478851

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0019393
Physical Location & Seq
Reverse (-)
24489457 .. 24490857
1401 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1401 bp
ATGAAGCTTGAAGTGCAAGTAAGCTCTAAGGAGAATATCAAACCATCTTCTCCAACCCCCGGCCATCTTCGCTATCACCCCTTGTCTTTTCTTGATCAAATCAATAGCCCATCCTACTTCTGTTTTGTGCACTTCTATGAACTGAATGGAGGAGACTCACAAGTGACACAACCTGAAACTAGCGAGATATCCGGCCATCTCAAGAAGTCGTTAGCCGAGGTCTTAACCCTTTTCTACCCACTCGCTGGACGACTCAAAGACAACCTTTATGTTCACTGCAATGATGAGGGCATAGAGTACCTTGAAGCTCGAGTCAACAAGACCACGCTTTCCGACCTTCTCGACGATCCCATCCCTGGTGAACTCACCAAACTCGTCCCATTTGAAGGAGATGTTAATGAATTCTCCCTCGGTGTGCAGCTTAACATTTTTGAATGCGGTGGAGTCGCTATTGGGATTTCCTTTTCCCACAAGCTTACAGATGGGTTATCTATGCTCATGTTCATCAAAGCTTGGGCTGCCACTTCCCGACGTGCCCTCGGAGATCATCAAGCTGAAATCGAGCCTCCACAGTTTGTTTCAGCAACATTCTTCCCGCCAAAGATATTCACACAACACGATGCAAGCTTTGGTATGAGAAAGAATAAAGTGACAACAAAAAGGTTTGTGTTTGATGCCGCCAACATTGAGGACCTCAGAGCAAAATATGTCGACCTAGACAAACCGTTGATCAAGCGCCCCTCGCGTGTCGAGTCCTTATCAGCTTTTCTATGGAGCCGAATAGTGGCTGCTTCGAAAGATGATGATCATGTTGACGACAAGTTGTATAGAGTGATGAATGTTGTCAATCTTCGTCCACTGTTCAATCCACCGTTGCCGCAATCTGTGTTTGGAAACCTCGTCGGGATTTCCATAACATCTCCAACATCCTCGGGGGCAGAATATTCTAGTGGTTTCATCAAGCACTCACGAGAGCAAATAAGCAACATTACCGACGACTACGTGAAAAAACTTCATCAGTGTGATGAGCATTTGGGTTTTCTGGAGAGATATGCTGATGTTTATAAGAGAGGAGAGAGTACCGTGACGTCCATGTTCAGTAGTATATGCAAGTTTCCTCTGTATGACAGTGACTTTGGTTGGGGAAGACCAACGTATGTGGGCGTTCCGGCGTTCAGCTTCGCGAACACGATCGTTTTCGTCGACCCCAAAGAGGGTGGTGGAATAGAGGTCCACGTTTGCTTGAGGGAAGAGGTCATGGTTAAATTTGAAAGGGACCCCAAGTTGCTCTCACGGCTGAGTTTGAGTTCGGTTGGTTCGGGTTCAATCAAAAAACCTATTCAGACTAGATCGGTAAAGTCTGGATCGGGGGGATTTAAGGGTTTGCTCTCACGATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

466

Amino Acids

51.99

Weight (kDa)

6.68

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 428 3.5e-75 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019393)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0478851
rosa_laevigata RLG00000023620
rosa_multiflora Rmu_co8298385.1_g000001 Rmu_sc0004831.1_g000009 Rmu_sc0005751.1_g000007
rosa_roxburghii Rroxscaffold_6G00403020
rosa_rugosa Rorug03G0168700
rosa_wichuraiana Rw3G019810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1065
AatII GACGTC 1 cut(s) 1091
AccB7I CCANNNNNTGG 1 cut(s) 245
AccI GTMKAC 2 cut(s) 711, 1203
AccII CGCG 2 cut(s) 745, 1184
AciI CCGC 4 cut(s) 438, 596, 678, 878
AclWI GGATC 2 cut(s) 341, 1372
AcoI YGGCCR 2 cut(s) 61, 193
AcsI RAATTY 2 cut(s) 401, 1265
AcyI GRCGYC 1 cut(s) 1088
AfaI GTAC 2 cut(s) 299, 1081
AfiI CCNNNNNNNGG 7 cut(s) 59, 245, 356, 386, 784, 1213, 1214
AgsI TTSAA 7 cut(s) 11, 305, 386, 434, 865, 1271, 1326
AjiI CACGTC 1 cut(s) 533
AjnI CCWGG 1 cut(s) 355
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 1 cut(s) 147
Alw44I GTGCAC 1 cut(s) 128
AlwI GGATC 2 cut(s) 341, 1372
Ama87I CYCGRG 2 cut(s) 309, 931
AoxI GGCC 2 cut(s) 61, 193
ApaLI GTGCAC 1 cut(s) 128
ApeKI GCWGC 3 cut(s) 418, 518, 788
ApoI RAATTY 2 cut(s) 401, 1265
ArsI GACNNNNNNTTYG 2 cut(s) 1118, 1150
AspLEI GCGC 1 cut(s) 738
AspS9I GGNCC 3 cut(s) 691, 1231, 1276
AsuC2I CCSGG 1 cut(s) 60
AsuHPI GGTGA 3 cut(s) 68, 358, 371
AsuII TTCGAA 1 cut(s) 794
AvaI CYCGRG 2 cut(s) 309, 931
AvaII GGWCC 3 cut(s) 691, 1231, 1276
BaeGI GKGCMC 2 cut(s) 132, 538
BauI CACGAG 1 cut(s) 969
BbsI GAAGAC 1 cut(s) 1153
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 3 cut(s) 430, 505, 775
BccI CCATC 6 cut(s) 52, 72, 118, 204, 359, 476
BceAI ACGGC 1 cut(s) 1310
BciT130I CCWGG 1 cut(s) 357
BclI TGATCA 3 cut(s) 94, 729, 805
BcnI CCSGG 1 cut(s) 60
BcoDI GTCTC 1 cut(s) 147
BfaI CTAG 4 cut(s) 180, 716, 948, 1347
BfoI RGCGCY 1 cut(s) 739
BisI GCNGC 5 cut(s) 419, 519, 678, 789, 878
BlsI GCNGC 5 cut(s) 420, 520, 679, 790, 879
Bme1390I CCNGG 2 cut(s) 60, 357
Bme18I GGWCC 3 cut(s) 691, 1231, 1276
BmeT110I CYCGRG 2 cut(s) 309, 931
BmgBI CACGTC 1 cut(s) 533
BmgT120I GGNCC 3 cut(s) 691, 1231, 1276
BmiI GGNNCC 3 cut(s) 776, 1277, 1278
BmrFI CCNGG 2 cut(s) 60, 357
BmsI GCATC 2 cut(s) 610, 664
BpiI GAAGAC 1 cut(s) 1153
BpmI CTGGAG 1 cut(s) 1064
Bpu14I TTCGAA 1 cut(s) 794
BpuEI CTTGAG 2 cut(s) 185, 1264
BpuMI CCSGG 1 cut(s) 60
BsaAI YACGTR 1 cut(s) 1003
BsaBI GATNNNNATC 1 cut(s) 804
BsaHI GRCGYC 1 cut(s) 1088
BsaJI CCNNGG 6 cut(s) 58, 216, 355, 409, 538, 930
Bsc4I CCNNNNNNNGG 7 cut(s) 59, 245, 356, 386, 784, 1213, 1214
Bse3DI GCAATG 1 cut(s) 286
Bse8I GATNNNNATC 1 cut(s) 804
BseBI CCWGG 1 cut(s) 357
BseDI CCNNGG 6 cut(s) 58, 216, 355, 409, 538, 930
BseGI GGATG 3 cut(s) 110, 351, 926
BseJI GATNNNNATC 1 cut(s) 804
BseLI CCNNNNNNNGG 7 cut(s) 59, 245, 356, 386, 784, 1213, 1214
BseMI GCAATG 1 cut(s) 286
BseMII CTCAG 2 cut(s) 709, 1289
BseRI GAGGAG 2 cut(s) 165, 1086
BseSI GKGCMC 2 cut(s) 132, 538
BseXI GCAGC 3 cut(s) 430, 505, 775
BsgI GTGCAG 1 cut(s) 437
Bsh1236I CGCG 2 cut(s) 745, 1184
Bsh1285I CGRYCG 1 cut(s) 1194
BshFI GGCC 2 cut(s) 63, 195
BsiEI CGRYCG 1 cut(s) 1194
BsiHKAI GWGCWC 1 cut(s) 132
BsiHKCI CYCGRG 2 cut(s) 309, 931
BsiSI CCGG 3 cut(s) 60, 192, 1169
BslFI GGGAC 2 cut(s) 362, 1289
BslI CCNNNNNNNGG 7 cut(s) 59, 245, 356, 386, 784, 1213, 1214
BsmAI GTCTC 1 cut(s) 147
BsmFI GGGAC 2 cut(s) 362, 1289
BsmI GAATGC 1 cut(s) 440
BsnI GGCC 2 cut(s) 63, 195
BsoBI CYCGRG 2 cut(s) 309, 931
Bsp119I TTCGAA 1 cut(s) 794
Bsp1286I GDGCHC 2 cut(s) 132, 538
Bsp143I GATC 8 cut(s) 94, 346, 544, 729, 805, 1191, 1349, 1364
Bsp68I TCGCGA 1 cut(s) 1184
BspACI CCGC 4 cut(s) 438, 596, 678, 878
BspANI GGCC 2 cut(s) 63, 195
BspCNI CTCAG 2 cut(s) 708, 1290
BspFNI CGCG 2 cut(s) 745, 1184
BspLI GGNNCC 3 cut(s) 776, 1277, 1278
BspPI GGATC 2 cut(s) 341, 1372
BspT104I TTCGAA 1 cut(s) 794
BsrDI GCAATG 1 cut(s) 286
BssECI CCNNGG 6 cut(s) 58, 216, 355, 409, 538, 930
BssMI GATC 8 cut(s) 94, 346, 544, 729, 805, 1191, 1349, 1364
BssNI GRCGYC 1 cut(s) 1088
BssSI CACGAG 1 cut(s) 969
Bst2BI CACGAG 1 cut(s) 969
Bst2UI CCWGG 1 cut(s) 357
Bst4CI ACNGT 6 cut(s) 573, 726, 861, 873, 1084, 1130
Bst6I CTCTTC 1 cut(s) 1245
BstACI GRCGYC 1 cut(s) 1088
BstBAI YACGTR 1 cut(s) 1003
BstBI TTCGAA 1 cut(s) 794
BstC8I GCNNGC 1 cut(s) 625
BstDEI CTNAG 3 cut(s) 27, 695, 1298
BstF5I GGATG 3 cut(s) 110, 351, 926
BstFNI CGCG 2 cut(s) 745, 1184
BstH2I RGCGCY 1 cut(s) 739
BstHHI GCGC 1 cut(s) 738
BstKTI GATC 8 cut(s) 97, 349, 547, 732, 808, 1194, 1352, 1367
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 8 cut(s) 94, 346, 544, 729, 805, 1191, 1349, 1364
BstMCI CGRYCG 1 cut(s) 1194
BstMWI GCNNNNNNNGC 5 cut(s) 13, 69, 518, 742, 1294
BstNI CCWGG 1 cut(s) 357
BstSCI CCNGG 2 cut(s) 58, 355
BstSLI GKGCMC 2 cut(s) 132, 538
BstUI CGCG 2 cut(s) 745, 1184
BstV1I GCAGC 3 cut(s) 430, 505, 775
BstV2I GAAGAC 1 cut(s) 1153
BsuRI GGCC 2 cut(s) 63, 195
BtrI CACGTC 1 cut(s) 533
BtsCI GGATG 3 cut(s) 110, 351, 926
BtsI GCAGTG 1 cut(s) 274
BtsIMutI CAGTG 4 cut(s) 274, 857, 1025, 1135
BtuMI TCGCGA 1 cut(s) 1184
Cac8I GCNNGC 1 cut(s) 625
CfoI GCGC 1 cut(s) 738
Cfr13I GGNCC 3 cut(s) 691, 1231, 1276
Csp6I GTAC 2 cut(s) 298, 1080
CspCI CAANNNNNGTGG 4 cut(s) 1140, 1175, 1198, 1233
CviAII CATG 4 cut(s) 499, 809, 1093, 1258
CviQI GTAC 2 cut(s) 298, 1080
DdeI CTNAG 3 cut(s) 27, 695, 1298
DpnI GATC 8 cut(s) 96, 348, 546, 731, 807, 1193, 1351, 1366
DpnII GATC 8 cut(s) 94, 346, 544, 729, 805, 1191, 1349, 1364
EaeI YGGCCR 2 cut(s) 61, 193
Eam1104I CTCTTC 1 cut(s) 1245
EarI CTCTTC 1 cut(s) 1245
Eco32I GATATC 1 cut(s) 189
Eco47I GGWCC 3 cut(s) 691, 1231, 1276
Eco88I CYCGRG 2 cut(s) 309, 931
EcoO109I RGGNCCY 2 cut(s) 691, 1276
EcoRI GAATTC 1 cut(s) 401
EcoRII CCWGG 1 cut(s) 355
EcoRV GATATC 1 cut(s) 189
FaeI CATG 4 cut(s) 502, 812, 1096, 1261
FalI AAGNNNNNCTT 2 cut(s) 249, 281
FaqI GGGAC 2 cut(s) 362, 1289
FatI CATG 4 cut(s) 498, 808, 1092, 1257
FauI CCCGC 1 cut(s) 603
FbaI TGATCA 3 cut(s) 94, 729, 805
FblI GTMKAC 2 cut(s) 711, 1203
Fnu4HI GCNGC 5 cut(s) 419, 519, 678, 789, 878
FokI GGATG 3 cut(s) 97, 338, 913
Fsp4HI GCNGC 5 cut(s) 419, 519, 678, 789, 878
FspBI CTAG 4 cut(s) 180, 716, 948, 1347
GlaI GCGC 1 cut(s) 737
GluI GCNGC 5 cut(s) 419, 519, 678, 789, 878
GsuI CTGGAG 1 cut(s) 1064
HaeII RGCGCY 1 cut(s) 739
HaeIII GGCC 2 cut(s) 63, 195
HapII CCGG 3 cut(s) 60, 192, 1169
HhaI GCGC 1 cut(s) 738
Hin1I GRCGYC 1 cut(s) 1088
Hin1II CATG 4 cut(s) 502, 812, 1096, 1261
Hin6I GCGC 1 cut(s) 736
HinP1I GCGC 1 cut(s) 736
HincII GTYRAC 4 cut(s) 316, 712, 814, 1204
HindII GTYRAC 4 cut(s) 316, 712, 814, 1204
HindIII AAGCTT 4 cut(s) 5, 473, 510, 625
HinfI GANTC 5 cut(s) 155, 252, 312, 444, 752
HpaII CCGG 3 cut(s) 60, 192, 1169
HphI GGTGA 3 cut(s) 68, 358, 371
Hpy166II GTNNAC 9 cut(s) 130, 274, 316, 362, 712, 814, 857, 1204, 1234
Hpy188I TCNGA 4 cut(s) 334, 542, 698, 1344
Hpy8I GTNNAC 9 cut(s) 130, 274, 316, 362, 712, 814, 857, 1204, 1234
Hpy99I CGWCG 5 cut(s) 347, 534, 905, 998, 1205
HpyAV CCTTC 2 cut(s) 347, 380
HpyCH4III ACNGT 6 cut(s) 573, 726, 861, 873, 1084, 1130
HpyCH4IV ACGT 5 cut(s) 532, 1002, 1088, 1154, 1236
HpyCH4V TGCA 6 cut(s) 16, 130, 279, 418, 623, 1110
HpyF10VI GCNNNNNNNGC 5 cut(s) 13, 69, 518, 742, 1294
HpyF3I CTNAG 3 cut(s) 27, 695, 1298
HpySE526I ACGT 5 cut(s) 532, 1002, 1088, 1154, 1236
Hsp92I GRCGYC 1 cut(s) 1088
Hsp92II CATG 4 cut(s) 502, 812, 1096, 1261
HspAI GCGC 1 cut(s) 736
KflI GGGWCCC 1 cut(s) 1276
Ksp22I TGATCA 3 cut(s) 94, 729, 805
Kzo9I GATC 8 cut(s) 94, 346, 544, 729, 805, 1191, 1349, 1364
LmnI GCTCC 1 cut(s) 774
LpnPI CCDG 9 cut(s) 73, 186, 205, 231, 342, 369, 1028, 1182, 1347
Lsp1109I GCAGC 3 cut(s) 430, 505, 775
LweI GCATC 2 cut(s) 610, 664
MaeI CTAG 4 cut(s) 180, 716, 948, 1347
MaeII ACGT 5 cut(s) 532, 1002, 1088, 1154, 1236
MaeIII GTNAC 4 cut(s) 163, 649, 1084, 1130
MalI GATC 8 cut(s) 96, 348, 546, 731, 807, 1193, 1351, 1366
MboI GATC 8 cut(s) 94, 346, 544, 729, 805, 1191, 1349, 1364
MboII GAAGA 6 cut(s) 39, 59, 583, 842, 1158, 1262
MhlI GDGCHC 2 cut(s) 132, 538
MluCI AATT 2 cut(s) 401, 1265
MlyI GAGTC 5 cut(s) 149, 246, 321, 453, 761
MmeI TCCRAC 3 cut(s) 77, 357, 947
MseI TTAA 5 cut(s) 224, 396, 423, 1263, 1377
MslI CAYNNNNRTG 3 cut(s) 135, 279, 1020
MspI CCGG 3 cut(s) 60, 192, 1169
MspR9I CCNGG 2 cut(s) 60, 357
Mva1269I GAATGC 1 cut(s) 440
MvaI CCWGG 1 cut(s) 357
MvnI CGCG 2 cut(s) 745, 1184
MwoI GCNNNNNNNGC 5 cut(s) 13, 69, 518, 742, 1294
NciI CCSGG 1 cut(s) 60
NdeII GATC 8 cut(s) 94, 346, 544, 729, 805, 1191, 1349, 1364
NlaIII CATG 4 cut(s) 502, 812, 1096, 1261
NlaIV GGNNCC 3 cut(s) 776, 1277, 1278
NmeAIII GCCGAG 1 cut(s) 241
NmuCI GTSAC 4 cut(s) 163, 649, 1084, 1130
NruI TCGCGA 1 cut(s) 1184
NspV TTCGAA 1 cut(s) 794
PaeR7I CTCGAG 1 cut(s) 309
PcsI WCGNNNNNNNCGW 1 cut(s) 1200
PctI GAATGC 1 cut(s) 440
PflMI CCANNNNNTGG 1 cut(s) 245
PkrI GCNGC 5 cut(s) 420, 520, 679, 790, 879
Ple19I CGATCG 1 cut(s) 1194
PleI GAGTC 5 cut(s) 149, 246, 320, 452, 760
PpsI GAGTC 5 cut(s) 149, 246, 320, 452, 760
Ppu21I YACGTR 1 cut(s) 1003
PpuMI RGGWCCY 2 cut(s) 691, 1276
PsiI TTATAA 1 cut(s) 1065
Psp5II RGGWCCY 2 cut(s) 691, 1276
Psp6I CCWGG 1 cut(s) 355
PspGI CCWGG 1 cut(s) 355
PspN4I GGNNCC 3 cut(s) 776, 1277, 1278
PspPI GGNCC 3 cut(s) 691, 1231, 1276
PspPPI RGGWCCY 2 cut(s) 691, 1276
PspXI VCTCGAGB 1 cut(s) 309
PvuI CGATCG 1 cut(s) 1194
RruI TCGCGA 1 cut(s) 1184
RsaI GTAC 2 cut(s) 299, 1081
RsaNI GTAC 2 cut(s) 298, 1080
RseI CAYNNNNRTG 3 cut(s) 135, 279, 1020
SalI GTCGAC 2 cut(s) 710, 1202
SaqAI TTAA 5 cut(s) 224, 396, 423, 1263, 1377
SatI GCNGC 5 cut(s) 419, 519, 678, 789, 878
Sau3AI GATC 8 cut(s) 94, 346, 544, 729, 805, 1191, 1349, 1364
Sau96I GGNCC 3 cut(s) 691, 1231, 1276
SchI GAGTC 5 cut(s) 149, 246, 321, 453, 761
ScrFI CCNGG 2 cut(s) 60, 357
SduI GDGCHC 2 cut(s) 132, 538
SfaNI GCATC 2 cut(s) 610, 664
Sfr274I CTCGAG 1 cut(s) 309
SfuI TTCGAA 1 cut(s) 794
SinI GGWCC 3 cut(s) 691, 1231, 1276
SlaI CTCGAG 1 cut(s) 309
SmiMI CAYNNNNRTG 3 cut(s) 135, 279, 1020
SmlI CTYRAG 3 cut(s) 200, 309, 1243
SmoI CTYRAG 3 cut(s) 200, 309, 1243
Sse9I AATT 2 cut(s) 401, 1265
SsiI CCGC 4 cut(s) 438, 596, 678, 878
SspI AATATT 1 cut(s) 944
SspMI CTAG 4 cut(s) 180, 716, 948, 1347
StyD4I CCNGG 2 cut(s) 58, 355
TaaI ACNGT 6 cut(s) 573, 726, 861, 873, 1084, 1130
TaiI ACGT 5 cut(s) 535, 1005, 1091, 1157, 1239
TaqI TCGA 7 cut(s) 310, 342, 561, 711, 750, 794, 1203
TasI AATT 2 cut(s) 401, 1265
TauI GCSGC 2 cut(s) 680, 880
Tru1I TTAA 5 cut(s) 224, 396, 423, 1263, 1377
Tru9I TTAA 5 cut(s) 224, 396, 423, 1263, 1377
TscAI CASTG 4 cut(s) 281, 864, 1025, 1135
TseFI GTSAC 4 cut(s) 163, 649, 1084, 1130
TseI GCWGC 3 cut(s) 418, 518, 788
Tsp45I GTSAC 4 cut(s) 163, 649, 1084, 1130
TspDTI ATGAA 7 cut(s) 17, 153, 414, 493, 851, 946, 1004
TspRI CASTG 4 cut(s) 281, 864, 1025, 1135
Van91I CCANNNNNTGG 1 cut(s) 245
VneI GTGCAC 1 cut(s) 128
VpaK11BI GGWCC 3 cut(s) 691, 1231, 1276
XapI RAATTY 2 cut(s) 401, 1265
XhoI CTCGAG 1 cut(s) 309
XmiI GTMKAC 2 cut(s) 711, 1203
XspI CTAG 4 cut(s) 180, 716, 948, 1347
ZraI GACGTC 1 cut(s) 1089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.