Rroxscaffold_6G00403020

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0019393
Physical Location & Seq
Forward (+)
25496353 .. 25496937
585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00403020.1

Sequence Viewer

Length: 585 bp
ATGAATTCTCCCTTGGTGTGCAGCTTAACATTTTTGAATGCGGTGGAGTCGCTATTGGGATTTCCTTTTCCCACAAGCTTACGGATGGGTTATCTATGCTCATGTTCATCAAAGCTTGGGCTGCCACTTCCCGACGTGCCCTCGGAGATCATCAAGTTGAAATCGAGCCTCCCACAGTTTGTTTCGACAACATTCTTCCCACCAAAGATATTCACACAACACGATGCAAGCTTTGGTATGAGAAAGAATAAAGTGACAACAAAAAGGTTTGTGTTTGATGCCGCCAACATTGAGGACCTCGCAGAAAAATATGTCGACCTAGACAAACCGTTGATCAAGCGCCCCTCGCGTGTCGAGTCCTTGTCGGCTTTTCTATGGAGCCGAATAGTGGTCGCTTCGAAAGATGATGATCATGTTGACGACAAGTTGTATAGAGTGATGAATGTTGTCAATCTTCGTCCACTGTTCAATCCACCGTTGCCGCAATCTGTGTTTGGAAACCTCGTCGGGATTTCCATAACATCTCCGACATCCTCGGGGGCGAATATTCTAGTGGTTTCATCAAGCACTCACGAGAGCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

194

Amino Acids

21.42

Weight (kDa)

9.01

Isoelectric Point (pI)

53.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 66 - 174 3.4e-11 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019393)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0478851
rosa_laevigata RLG00000023620
rosa_multiflora Rmu_co8298385.1_g000001 Rmu_sc0004831.1_g000009 Rmu_sc0005751.1_g000007
rosa_roxburghii Rroxscaffold_6G00403020
rosa_rugosa Rorug03G0168700
rosa_wichuraiana Rw3G019810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 315
AccII CGCG 1 cut(s) 349
AciI CCGC 3 cut(s) 41, 282, 482
AcsI RAATTY 1 cut(s) 4
AfiI CCNNNNNNNGG 1 cut(s) 388
AgsI TTSAA 3 cut(s) 37, 160, 469
AjiI CACGTC 1 cut(s) 136
AjuI GAANNNNNNNTTGG 1 cut(s) 28
AluBI AGCT 4 cut(s) 24, 78, 115, 231
AluI AGCT 4 cut(s) 24, 78, 115, 231
Ama87I CYCGRG 1 cut(s) 535
ApeKI GCWGC 2 cut(s) 21, 121
ApoI RAATTY 1 cut(s) 4
AspLEI GCGC 1 cut(s) 342
AspS9I GGNCC 1 cut(s) 295
AsuII TTCGAA 1 cut(s) 398
AvaI CYCGRG 1 cut(s) 535
AvaII GGWCC 1 cut(s) 295
BaeGI GKGCMC 1 cut(s) 141
BauI CACGAG 1 cut(s) 572
BbvI GCAGC 2 cut(s) 33, 108
BccI CCATC 1 cut(s) 79
BclI TGATCA 2 cut(s) 333, 409
BfaI CTAG 2 cut(s) 320, 551
BfoI RGCGCY 1 cut(s) 343
BisI GCNGC 4 cut(s) 22, 122, 282, 482
BlsI GCNGC 4 cut(s) 23, 123, 283, 483
Bme18I GGWCC 1 cut(s) 295
BmeT110I CYCGRG 1 cut(s) 535
BmgBI CACGTC 1 cut(s) 136
BmgT120I GGNCC 1 cut(s) 295
BmiI GGNNCC 1 cut(s) 380
BmsI GCATC 2 cut(s) 214, 268
Bpu14I TTCGAA 1 cut(s) 398
BsaBI GATNNNNATC 1 cut(s) 408
BsaJI CCNNGG 3 cut(s) 12, 141, 534
Bsc4I CCNNNNNNNGG 1 cut(s) 388
Bse8I GATNNNNATC 1 cut(s) 408
BseDI CCNNGG 3 cut(s) 12, 141, 534
BseGI GGATG 2 cut(s) 90, 530
BseJI GATNNNNATC 1 cut(s) 408
BseLI CCNNNNNNNGG 1 cut(s) 388
BseSI GKGCMC 1 cut(s) 141
BseXI GCAGC 2 cut(s) 33, 108
BsgI GTGCAG 1 cut(s) 40
Bsh1236I CGCG 1 cut(s) 349
BsiHKCI CYCGRG 1 cut(s) 535
BslI CCNNNNNNNGG 1 cut(s) 388
BsmI GAATGC 1 cut(s) 43
BsoBI CYCGRG 1 cut(s) 535
Bsp119I TTCGAA 1 cut(s) 398
Bsp1286I GDGCHC 1 cut(s) 141
Bsp143I GATC 3 cut(s) 147, 333, 409
BspACI CCGC 3 cut(s) 41, 282, 482
BspFNI CGCG 1 cut(s) 349
BspLI GGNNCC 1 cut(s) 380
BspT104I TTCGAA 1 cut(s) 398
BssECI CCNNGG 3 cut(s) 12, 141, 534
BssMI GATC 3 cut(s) 147, 333, 409
BssSI CACGAG 1 cut(s) 572
BssT1I CCWWGG 1 cut(s) 12
Bst2BI CACGAG 1 cut(s) 572
Bst4CI ACNGT 4 cut(s) 177, 330, 465, 477
BstBI TTCGAA 1 cut(s) 398
BstC8I GCNNGC 1 cut(s) 229
BstF5I GGATG 2 cut(s) 90, 530
BstFNI CGCG 1 cut(s) 349
BstH2I RGCGCY 1 cut(s) 343
BstHHI GCGC 1 cut(s) 342
BstKTI GATC 3 cut(s) 150, 336, 412
BstMBI GATC 3 cut(s) 147, 333, 409
BstMWI GCNNNNNNNGC 2 cut(s) 121, 346
BstSLI GKGCMC 1 cut(s) 141
BstUI CGCG 1 cut(s) 349
BstV1I GCAGC 2 cut(s) 33, 108
BtrI CACGTC 1 cut(s) 136
BtsCI GGATG 2 cut(s) 90, 530
BtsIMutI CAGTG 1 cut(s) 461
Cac8I GCNNGC 1 cut(s) 229
CfoI GCGC 1 cut(s) 342
Cfr13I GGNCC 1 cut(s) 295
CviAII CATG 2 cut(s) 102, 413
CviJI RGCY 8 cut(s) 24, 78, 115, 121, 168, 231, 368, 381
CviKI_1 RGCY 8 cut(s) 24, 78, 115, 121, 168, 231, 368, 381
DpnI GATC 3 cut(s) 149, 335, 411
DpnII GATC 3 cut(s) 147, 333, 409
Eco130I CCWWGG 1 cut(s) 12
Eco47I GGWCC 1 cut(s) 295
Eco88I CYCGRG 1 cut(s) 535
EcoO109I RGGNCCY 1 cut(s) 295
EcoRI GAATTC 1 cut(s) 4
EcoT14I CCWWGG 1 cut(s) 12
ErhI CCWWGG 1 cut(s) 12
FaeI CATG 2 cut(s) 105, 416
FaiI YATR 8 cut(s) 97, 103, 239, 312, 376, 414, 432, 518
FatI CATG 2 cut(s) 101, 412
FbaI TGATCA 2 cut(s) 333, 409
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 4 cut(s) 22, 122, 282, 482
FokI GGATG 2 cut(s) 97, 517
Fsp4HI GCNGC 4 cut(s) 22, 122, 282, 482
FspBI CTAG 2 cut(s) 320, 551
GlaI GCGC 1 cut(s) 341
GluI GCNGC 4 cut(s) 22, 122, 282, 482
HaeII RGCGCY 1 cut(s) 343
HhaI GCGC 1 cut(s) 342
Hin1II CATG 2 cut(s) 105, 416
Hin6I GCGC 1 cut(s) 340
HinP1I GCGC 1 cut(s) 340
HincII GTYRAC 2 cut(s) 316, 418
HindII GTYRAC 2 cut(s) 316, 418
HindIII AAGCTT 3 cut(s) 76, 113, 229
HinfI GANTC 2 cut(s) 47, 356
Hpy166II GTNNAC 3 cut(s) 316, 418, 461
Hpy188I TCNGA 2 cut(s) 145, 528
Hpy188III TCNNGA 3 cut(s) 131, 508, 572
Hpy8I GTNNAC 3 cut(s) 316, 418, 461
Hpy99I CGWCG 2 cut(s) 137, 509
HpyCH4III ACNGT 4 cut(s) 177, 330, 465, 477
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 2 cut(s) 21, 227
HpyF10VI GCNNNNNNNGC 2 cut(s) 121, 346
HpySE526I ACGT 1 cut(s) 135
Hsp92II CATG 2 cut(s) 105, 416
HspAI GCGC 1 cut(s) 340
Ksp22I TGATCA 2 cut(s) 333, 409
Kzo9I GATC 3 cut(s) 147, 333, 409
LmnI GCTCC 1 cut(s) 378
Lsp1109I GCAGC 2 cut(s) 33, 108
LweI GCATC 2 cut(s) 214, 268
MaeI CTAG 2 cut(s) 320, 551
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 1 cut(s) 253
MalI GATC 3 cut(s) 149, 335, 411
MboI GATC 3 cut(s) 147, 333, 409
MboII GAAGA 2 cut(s) 187, 446
MhlI GDGCHC 1 cut(s) 141
MluCI AATT 1 cut(s) 4
MlyI GAGTC 2 cut(s) 56, 365
MmeI TCCRAC 1 cut(s) 551
MnlI CCTC 7 cut(s) 151, 179, 286, 308, 355, 512, 544
MseI TTAA 1 cut(s) 26
Mva1269I GAATGC 1 cut(s) 43
MvnI CGCG 1 cut(s) 349
MwoI GCNNNNNNNGC 2 cut(s) 121, 346
NdeII GATC 3 cut(s) 147, 333, 409
NlaIII CATG 2 cut(s) 105, 416
NlaIV GGNNCC 1 cut(s) 380
NmuCI GTSAC 1 cut(s) 253
NspV TTCGAA 1 cut(s) 398
PctI GAATGC 1 cut(s) 43
PkrI GCNGC 4 cut(s) 23, 123, 283, 483
PleI GAGTC 2 cut(s) 55, 364
PpsI GAGTC 2 cut(s) 55, 364
PpuMI RGGWCCY 1 cut(s) 295
Psp5II RGGWCCY 1 cut(s) 295
PspN4I GGNNCC 1 cut(s) 380
PspPI GGNCC 1 cut(s) 295
PspPPI RGGWCCY 1 cut(s) 295
SalI GTCGAC 1 cut(s) 314
SaqAI TTAA 1 cut(s) 26
SatI GCNGC 4 cut(s) 22, 122, 282, 482
Sau3AI GATC 3 cut(s) 147, 333, 409
Sau96I GGNCC 1 cut(s) 295
SchI GAGTC 2 cut(s) 56, 365
SduI GDGCHC 1 cut(s) 141
SetI ASST 9 cut(s) 26, 80, 117, 138, 233, 269, 300, 321, 504
SfaNI GCATC 2 cut(s) 214, 268
SfuI TTCGAA 1 cut(s) 398
SinI GGWCC 1 cut(s) 295
Sse9I AATT 1 cut(s) 4
SsiI CCGC 3 cut(s) 41, 282, 482
SspI AATATT 1 cut(s) 547
SspMI CTAG 2 cut(s) 320, 551
StyI CCWWGG 1 cut(s) 12
TaaI ACNGT 4 cut(s) 177, 330, 465, 477
TaiI ACGT 1 cut(s) 138
TaqI TCGA 5 cut(s) 164, 185, 315, 354, 398
TasI AATT 1 cut(s) 4
TauI GCSGC 2 cut(s) 284, 484
Tru1I TTAA 1 cut(s) 26
Tru9I TTAA 1 cut(s) 26
TscAI CASTG 1 cut(s) 468
TseFI GTSAC 1 cut(s) 253
TseI GCWGC 2 cut(s) 21, 121
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 4 cut(s) 17, 96, 455, 549
TspGWI ACGGA 1 cut(s) 97
TspRI CASTG 1 cut(s) 468
VpaK11BI GGWCC 1 cut(s) 295
XapI RAATTY 1 cut(s) 4
XmiI GTMKAC 1 cut(s) 315
XspI CTAG 2 cut(s) 320, 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.