Rorug03G0168700

Vinorine synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0019393
Physical Location & Seq
Reverse (-)
14227859 .. 14229648
1790 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0168700.1

Sequence Viewer

Length: 966 bp
ATGGGGAGAGCTCCTTGCTGTGACAAGGATAATGTGAAGAAAGGACCATGGTCACCTGAAGAAGATGCAAAGCTTAAAGAGTATATAGAAAAGTATGGGACTGGAGGGAACTGGATTGCTCTTCCACAGAAAGCTGGTCTAAGGAGATGTGGAAAAAGTTGCAGATTAAGATGGCTTAACTATCTCAGGCCCAACATTAAACATGGAGAATTTTCTGATGAAGAAGATAAGATCATCTGCAACCTCTTTGCTAGCATTGGAAGCAGGTGGTCAATTATAGCAGCTCAATTGCCAGGAAGGACCGACAATGATATCAAGAACTACTGGAACACAAAGCTCAAGAAGAAACTCAACCAATATTCCATGATGGGCATAATTCCTCACCACTCCGCCACCTTCTCATCTCTTCTTCATCACCAAACTTCAACCTCATCATCTTCACCACCATCATACAGAAGCAGCAACAACACTAGTACTCCTACTTACTACACACATCAACCCAGGTCTTTCACCAGTACTGCTAATCTGGAATCCCATATTCCATTTTCACCAAGTCTTTTAAGTAGTACCAATACTACTACTAATGCTGCTCTTCAAGTGGGTCAAGAGAGTTATGTGAGTCAACCCTTGACGCAACATTATCAAGCCAAAGATAACATCTTGATGTTTGGGGGTACCACTACTGAAGCAAGCTGCAGTTCTTCTGATGGGAGCTATGGTACTGGTTATGGTGAGGTACAGTTGGGTCTGCAAAACTACTTCTACAATAATGGTGCTGAAGCAAACAACCAGAAAATCATGGTGTCAGATAATGGTCTTGGTCTTGATCATGACCAGGCTCCATTAGATTATGGCTTTGAAGAGATCAAGCAGTTAATTAGCAGTAGCACTACTACTAGTGGTGGTTGCAACAACTTTTTGTTTGATGAAAACAAGACAGAGGAAAAGGTCATGATGTACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110
Pfam Domains
Protein Families
Transferase

Protein Analysis

321

Amino Acids

35.75

Weight (kDa)

6.75

Isoelectric Point (pI)

51.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 62 2.1e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 76 4e-13 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 69 - 111 5.2e-14 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 71 - 119 2e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019393)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0478851
rosa_laevigata RLG00000023620
rosa_multiflora Rmu_co8298385.1_g000001 Rmu_sc0004831.1_g000009 Rmu_sc0005751.1_g000007
rosa_roxburghii Rroxscaffold_6G00403020
rosa_rugosa Rorug03G0168700
rosa_wichuraiana Rw3G019810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 255
Acc36I ACCTGC 1 cut(s) 255
Acc65I GGTACC 1 cut(s) 674
AccB1I GGYRCC 1 cut(s) 674
AciI CCGC 1 cut(s) 390
AcsI RAATTY 1 cut(s) 209
AcuI CTGAAG 3 cut(s) 78, 705, 798
AfaI GTAC 7 cut(s) 475, 517, 568, 676, 721, 738, 959
AgsI TTSAA 3 cut(s) 426, 596, 860
AhlI ACTAGT 2 cut(s) 470, 896
AjnI CCWGG 3 cut(s) 292, 500, 834
AluBI AGCT 7 cut(s) 11, 73, 134, 284, 337, 693, 714
AluI AGCT 7 cut(s) 11, 73, 134, 284, 337, 693, 714
Alw21I GWGCWC 1 cut(s) 13
AoxI GGCC 1 cut(s) 188
ApeKI GCWGC 4 cut(s) 281, 459, 587, 693
ApoI RAATTY 1 cut(s) 209
Asp718I GGTACC 1 cut(s) 674
AspS9I GGNCC 3 cut(s) 44, 189, 300
AsuHPI GGTGA 7 cut(s) 45, 374, 407, 432, 502, 540, 743
AsuNHI GCTAGC 1 cut(s) 251
AvaII GGWCC 2 cut(s) 44, 300
BanI GGYRCC 1 cut(s) 674
BanII GRGCYC 1 cut(s) 13
Bbv12I GWGCWC 1 cut(s) 13
BbvI GCAGC 4 cut(s) 293, 471, 574, 680
BccI CCATC 4 cut(s) 165, 361, 454, 701
BciT130I CCWGG 3 cut(s) 294, 502, 836
BclI TGATCA 1 cut(s) 826
BcuI ACTAGT 2 cut(s) 470, 896
BfaI CTAG 3 cut(s) 252, 471, 897
BfmI CTRYAG 1 cut(s) 694
BfuAI ACCTGC 1 cut(s) 255
BisI GCNGC 4 cut(s) 282, 460, 588, 694
BlsI GCNGC 4 cut(s) 283, 461, 589, 695
BmcAI AGTACT 2 cut(s) 475, 517
Bme1390I CCNGG 3 cut(s) 294, 502, 836
Bme18I GGWCC 2 cut(s) 44, 300
BmgT120I GGNCC 3 cut(s) 44, 189, 300
BmiI GGNNCC 2 cut(s) 676, 840
BmrFI CCNGG 3 cut(s) 294, 502, 836
BmsI GCATC 1 cut(s) 55
BmtI GCTAGC 1 cut(s) 255
BoxI GACNNNNGTC 1 cut(s) 49
BpmI CTGGAG 1 cut(s) 123
BpuEI CTTGAG 1 cut(s) 323
BsaJI CCNNGG 2 cut(s) 47, 500
BsaXI ACNNNNNCTCC 4 cut(s) 460, 490, 703, 733
Bse1I ACTGG 5 cut(s) 106, 116, 329, 513, 727
BseBI CCWGG 3 cut(s) 294, 502, 836
BseDI CCNNGG 2 cut(s) 47, 500
BseMII CTCAG 1 cut(s) 199
BseNI ACTGG 5 cut(s) 106, 116, 329, 513, 727
BseXI GCAGC 4 cut(s) 293, 471, 574, 680
BshFI GGCC 1 cut(s) 190
BshNI GGYRCC 1 cut(s) 674
BsiHKAI GWGCWC 1 cut(s) 13
BslFI GGGAC 1 cut(s) 112
BsmFI GGGAC 1 cut(s) 112
BsnI GGCC 1 cut(s) 190
Bsp1286I GDGCHC 1 cut(s) 13
Bsp143I GATC 3 cut(s) 231, 826, 864
Bsp19I CCATGG 1 cut(s) 47
BspACI CCGC 1 cut(s) 390
BspANI GGCC 1 cut(s) 190
BspCNI CTCAG 1 cut(s) 198
BspHI TCATGA 2 cut(s) 829, 951
BspLI GGNNCC 2 cut(s) 676, 840
BspMAI CTGCAG 1 cut(s) 698
BspMI ACCTGC 1 cut(s) 255
BspOI GCTAGC 1 cut(s) 255
BspQI GCTCTTC 2 cut(s) 126, 597
BspT107I GGYRCC 1 cut(s) 674
BsrI ACTGG 5 cut(s) 106, 116, 329, 513, 727
BssECI CCNNGG 2 cut(s) 47, 500
BssMI GATC 3 cut(s) 231, 826, 864
BssT1I CCWWGG 1 cut(s) 47
Bst2UI CCWGG 3 cut(s) 294, 502, 836
Bst4CI ACNGT 1 cut(s) 741
Bst6I CTCTTC 4 cut(s) 126, 411, 597, 855
BstC8I GCNNGC 2 cut(s) 253, 691
BstDEI CTNAG 2 cut(s) 140, 185
BstDSI CCRYGG 1 cut(s) 47
BstEII GGTNACC 1 cut(s) 51
BstKTI GATC 3 cut(s) 234, 829, 867
BstMBI GATC 3 cut(s) 231, 826, 864
BstMWI GCNNNNNNNGC 1 cut(s) 261
BstNI CCWGG 3 cut(s) 294, 502, 836
BstPAI GACNNNNGTC 1 cut(s) 49
BstPI GGTNACC 1 cut(s) 51
BstSCI CCNGG 3 cut(s) 292, 500, 834
BstSFI CTRYAG 1 cut(s) 694
BstV1I GCAGC 4 cut(s) 293, 471, 574, 680
BsuRI GGCC 1 cut(s) 190
BtgI CCRYGG 1 cut(s) 47
BveI ACCTGC 1 cut(s) 255
Cac8I GCNNGC 2 cut(s) 253, 691
CciI TCATGA 2 cut(s) 829, 951
Cfr13I GGNCC 3 cut(s) 44, 189, 300
CseI GACGC 1 cut(s) 640
Csp6I GTAC 7 cut(s) 474, 516, 567, 675, 720, 737, 958
CviAII CATG 6 cut(s) 48, 203, 364, 799, 830, 952
CviQI GTAC 7 cut(s) 474, 516, 567, 675, 720, 737, 958
DdeI CTNAG 2 cut(s) 140, 185
DpnI GATC 3 cut(s) 233, 828, 866
DpnII GATC 3 cut(s) 231, 826, 864
Eam1104I CTCTTC 4 cut(s) 126, 411, 597, 855
EarI CTCTTC 4 cut(s) 126, 411, 597, 855
EciI GGCGGA 1 cut(s) 379
Ecl136II GAGCTC 1 cut(s) 11
Eco130I CCWWGG 1 cut(s) 47
Eco24I GRGCYC 1 cut(s) 13
Eco32I GATATC 1 cut(s) 313
Eco47I GGWCC 2 cut(s) 44, 300
Eco53kI GAGCTC 1 cut(s) 11
Eco57I CTGAAG 3 cut(s) 78, 705, 798
Eco91I GGTNACC 1 cut(s) 51
EcoICRI GAGCTC 1 cut(s) 11
EcoO65I GGTNACC 1 cut(s) 51
EcoRII CCWGG 3 cut(s) 292, 500, 834
EcoRV GATATC 1 cut(s) 313
EcoT14I CCWWGG 1 cut(s) 47
EcoT38I GRGCYC 1 cut(s) 13
ErhI CCWWGG 1 cut(s) 47
FaeI CATG 6 cut(s) 51, 206, 367, 802, 833, 955
FaqI GGGAC 1 cut(s) 112
FatI CATG 6 cut(s) 47, 202, 363, 798, 829, 951
FbaI TGATCA 1 cut(s) 826
Fnu4HI GCNGC 4 cut(s) 282, 460, 588, 694
FriOI GRGCYC 1 cut(s) 13
Fsp4HI GCNGC 4 cut(s) 282, 460, 588, 694
FspBI CTAG 3 cut(s) 252, 471, 897
GluI GCNGC 4 cut(s) 282, 460, 588, 694
GsuI CTGGAG 1 cut(s) 123
HaeIII GGCC 1 cut(s) 190
HgaI GACGC 1 cut(s) 640
Hin1II CATG 6 cut(s) 51, 206, 367, 802, 833, 955
HincII GTYRAC 1 cut(s) 623
HindII GTYRAC 1 cut(s) 623
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 2 cut(s) 530, 619
HphI GGTGA 7 cut(s) 45, 374, 407, 432, 502, 540, 743
Hpy166II GTNNAC 1 cut(s) 623
Hpy188I TCNGA 3 cut(s) 217, 706, 808
Hpy188III TCNNGA 8 cut(s) 316, 340, 527, 605, 661, 824, 830, 952
Hpy8I GTNNAC 1 cut(s) 623
HpyAV CCTTC 2 cut(s) 291, 406
HpyCH4III ACNGT 1 cut(s) 741
HpyCH4V TGCA 6 cut(s) 68, 162, 240, 696, 751, 909
HpyF10VI GCNNNNNNNGC 1 cut(s) 261
HpyF3I CTNAG 2 cut(s) 140, 185
Hsp92II CATG 6 cut(s) 51, 206, 367, 802, 833, 955
KpnI GGTACC 1 cut(s) 678
Ksp22I TGATCA 1 cut(s) 826
Kzo9I GATC 3 cut(s) 231, 826, 864
LguI GCTCTTC 2 cut(s) 126, 597
LmnI GCTCC 3 cut(s) 16, 711, 844
Lsp1109I GCAGC 4 cut(s) 293, 471, 574, 680
LweI GCATC 1 cut(s) 55
MaeI CTAG 3 cut(s) 252, 471, 897
MaeIII GTNAC 2 cut(s) 20, 51
MalI GATC 3 cut(s) 233, 828, 866
MboI GATC 3 cut(s) 231, 826, 864
MfeI CAATTG 1 cut(s) 287
MhlI GDGCHC 1 cut(s) 13
MluCI AATT 5 cut(s) 209, 273, 287, 375, 876
MlyI GAGTC 1 cut(s) 628
MnlI CCTC 6 cut(s) 98, 254, 390, 439, 727, 934
MseI TTAA 6 cut(s) 75, 167, 177, 198, 560, 875
MslI CAYNNNNRTG 1 cut(s) 662
MspR9I CCNGG 3 cut(s) 294, 502, 836
MunI CAATTG 1 cut(s) 287
MvaI CCWGG 3 cut(s) 294, 502, 836
MwoI GCNNNNNNNGC 1 cut(s) 261
NcoI CCATGG 1 cut(s) 47
NdeII GATC 3 cut(s) 231, 826, 864
NheI GCTAGC 1 cut(s) 251
NlaIII CATG 6 cut(s) 51, 206, 367, 802, 833, 955
NlaIV GGNNCC 2 cut(s) 676, 840
NmuCI GTSAC 2 cut(s) 20, 51
PagI TCATGA 2 cut(s) 829, 951
PaqCI CACCTGC 1 cut(s) 255
PciSI GCTCTTC 2 cut(s) 126, 597
PfeI GAWTC 1 cut(s) 530
PkrI GCNGC 4 cut(s) 283, 461, 589, 695
PleI GAGTC 1 cut(s) 627
PpsI GAGTC 1 cut(s) 627
PshAI GACNNNNGTC 1 cut(s) 49
Psp124BI GAGCTC 1 cut(s) 13
Psp6I CCWGG 3 cut(s) 292, 500, 834
PspEI GGTNACC 1 cut(s) 51
PspGI CCWGG 3 cut(s) 292, 500, 834
PspN4I GGNNCC 2 cut(s) 676, 840
PspPI GGNCC 3 cut(s) 44, 189, 300
PstI CTGCAG 1 cut(s) 698
RsaI GTAC 7 cut(s) 475, 517, 568, 676, 721, 738, 959
RsaNI GTAC 7 cut(s) 474, 516, 567, 675, 720, 737, 958
RseI CAYNNNNRTG 1 cut(s) 662
SacI GAGCTC 1 cut(s) 13
SapI GCTCTTC 2 cut(s) 126, 597
SaqAI TTAA 6 cut(s) 75, 167, 177, 198, 560, 875
SatI GCNGC 4 cut(s) 282, 460, 588, 694
Sau3AI GATC 3 cut(s) 231, 826, 864
Sau96I GGNCC 3 cut(s) 44, 189, 300
ScaI AGTACT 2 cut(s) 475, 517
SchI GAGTC 1 cut(s) 628
ScrFI CCNGG 3 cut(s) 294, 502, 836
SduI GDGCHC 1 cut(s) 13
SfaNI GCATC 1 cut(s) 55
SfcI CTRYAG 1 cut(s) 694
SinI GGWCC 2 cut(s) 44, 300
SmiMI CAYNNNNRTG 1 cut(s) 662
SmlI CTYRAG 1 cut(s) 338
SmoI CTYRAG 1 cut(s) 338
SpeI ACTAGT 2 cut(s) 470, 896
Sse9I AATT 5 cut(s) 209, 273, 287, 375, 876
SsiI CCGC 1 cut(s) 390
SspI AATATT 1 cut(s) 359
SspMI CTAG 3 cut(s) 252, 471, 897
SstI GAGCTC 1 cut(s) 13
StyD4I CCNGG 3 cut(s) 292, 500, 834
StyI CCWWGG 1 cut(s) 47
TaaI ACNGT 1 cut(s) 741
TaqII GACCGA 1 cut(s) 317
TasI AATT 5 cut(s) 209, 273, 287, 375, 876
TatI WGTACW 3 cut(s) 473, 515, 957
TfiI GAWTC 1 cut(s) 530
Tru1I TTAA 6 cut(s) 75, 167, 177, 198, 560, 875
Tru9I TTAA 6 cut(s) 75, 167, 177, 198, 560, 875
TseFI GTSAC 2 cut(s) 20, 51
TseI GCWGC 4 cut(s) 281, 459, 587, 693
Tsp45I GTSAC 2 cut(s) 20, 51
TspDTI ATGAA 3 cut(s) 234, 401, 942
VpaK11BI GGWCC 2 cut(s) 44, 300
XapI RAATTY 1 cut(s) 209
XspI CTAG 3 cut(s) 252, 471, 897
ZrmI AGTACT 2 cut(s) 475, 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.