RchiOBHm_Chr3g0480141

Adagio protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0021377
Physical Location & Seq
Forward (+)
25917297 .. 25920193
2897 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 612 bp
ATGTTGTTTGACATTAGGCAACTATTTCAAGAGAGATATGCGAACTCTCTGGCTTATTGTGTGGATCAAAAGTTTTTCATTTGCTCTACTGCATCACAACCTCAGCTTTGGGAAGGCACAAGGTTGGGACATTCCCTTTCAGTTTATGGCCGGTCAAAGATTCTCATGTTTGGTGGACTTGCCAACAGTGGGCACTTGCGGTTAAGATCAGGTGAGACTTATACCATTGATTTGGAAGATGAAGAGCCTCAATGGAGGCAACTGGAGTGTAATGCATTTACCAGCATTGACAGCCAGAGTGCAGTGCTCCCTCCTCCTAGACTTGATCATGTTGCTGTGAGCATGCCTTGTGGAAGGATTATCATTTTTGGTGGTTCAATTGCCGGGCTGCATTCTCCTTCTCAGCTTTTCCTCTTGGATCCTTCTGAAGAGAAACCATCATGGAGAATTTTGAACGTTCCCGGGCAACCACCTAAATTTGCTTGGGGTCACAGCACCTGCGTGGTCGGAGGGACAAGGGTCCTGGTACTGGGTGGCCACACAGGTGAGGAGTGGATACTTAATGACTTGCATGAGTTGTGCTTAGCAAGCAGGCAGGATTCAGACCTATGA

Protein Analysis

203

Amino Acids

22.6

Weight (kDa)

5.6

Isoelectric Point (pI)

54.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_HCF PF13854 40 - 180 4.1e-07 Host cell factor, Kelch-repeats domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 43 - 199 2.5e-18 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 89 - 198 7.9e-07 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 91 - 198 4.4e-13 FBX42, beta-propeller domain
Kelch_2 PF07646 108 - 155 3.1e-08 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021377)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0480141
rosa_multiflora Rmu_sc0001549.1_g000001
rosa_samantha Rh3AG232200 Rh3CG262000
rosa_wichuraiana Rw3G020850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 506
Acc36I ACCTGC 1 cut(s) 506
AciI CCGC 1 cut(s) 199
AclI AACGTT 1 cut(s) 456
AclWI GGATC 3 cut(s) 72, 413, 426
AcoI YGGCCR 2 cut(s) 148, 535
AcsI RAATTY 2 cut(s) 447, 476
AcuI CTGAAG 1 cut(s) 447
AfaI GTAC 1 cut(s) 528
AfiI CCNNNNNNNGG 2 cut(s) 189, 529
AgsI TTSAA 3 cut(s) 29, 378, 454
AjnI CCWGG 1 cut(s) 522
AleI CACNNNNGTG 2 cut(s) 500, 543
AluBI AGCT 2 cut(s) 106, 406
AluI AGCT 2 cut(s) 106, 406
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 1 cut(s) 209
AlwI GGATC 3 cut(s) 72, 413, 426
AlwNI CAGNNNCTG 1 cut(s) 498
Ama87I CYCGRG 1 cut(s) 461
AoxI GGCC 2 cut(s) 148, 535
ApeKI GCWGC 1 cut(s) 388
ApoI RAATTY 2 cut(s) 447, 476
AspS9I GGNCC 1 cut(s) 520
AsuC2I CCSGG 3 cut(s) 385, 462, 463
AsuHPI GGTGA 2 cut(s) 224, 557
AvaI CYCGRG 1 cut(s) 461
AvaII GGWCC 1 cut(s) 520
BaeGI GKGCMC 1 cut(s) 195
BalI TGGCCA 1 cut(s) 537
BamHI GGATCC 1 cut(s) 418
Bbv12I GWGCWC 1 cut(s) 309
BbvCI CCTCAGC 1 cut(s) 102
BbvI GCAGC 1 cut(s) 375
BccI CCATC 1 cut(s) 445
BciT130I CCWGG 1 cut(s) 524
BciVI GTATCC 1 cut(s) 549
BclI TGATCA 1 cut(s) 325
BcnI CCSGG 3 cut(s) 385, 462, 463
BcoDI GTCTC 1 cut(s) 209
BfaI CTAG 1 cut(s) 318
BfuAI ACCTGC 1 cut(s) 506
BfuI GTATCC 1 cut(s) 549
BisI GCNGC 1 cut(s) 389
BlpI GCTNAGC 1 cut(s) 583
BlsI GCNGC 1 cut(s) 390
Bme1390I CCNGG 4 cut(s) 385, 462, 463, 524
Bme18I GGWCC 1 cut(s) 520
BmeT110I CYCGRG 1 cut(s) 461
BmgT120I GGNCC 1 cut(s) 520
BmiI GGNNCC 2 cut(s) 420, 521
BmrFI CCNGG 4 cut(s) 385, 462, 463, 524
BmrI ACTGGG 1 cut(s) 539
BmsI GCATC 1 cut(s) 101
BmuI ACTGGG 1 cut(s) 539
BoxI GACNNNNGTC 1 cut(s) 518
BpmI CTGGAG 1 cut(s) 284
Bpu10I CCTNAGC 1 cut(s) 102
Bpu1102I GCTNAGC 1 cut(s) 583
BpuMI CCSGG 3 cut(s) 385, 462, 463
BsaJI CCNNGG 1 cut(s) 461
Bsc4I CCNNNNNNNGG 2 cut(s) 189, 529
Bse118I RCCGGY 1 cut(s) 150
Bse1I ACTGG 2 cut(s) 267, 534
BseBI CCWGG 1 cut(s) 524
BseDI CCNNGG 1 cut(s) 461
BseLI CCNNNNNNNGG 2 cut(s) 189, 529
BseMII CTCAG 2 cut(s) 116, 416
BseNI ACTGG 2 cut(s) 267, 534
BseRI GAGGAG 2 cut(s) 303, 563
BseSI GKGCMC 1 cut(s) 195
BseXI GCAGC 1 cut(s) 375
BsgI GTGCAG 1 cut(s) 321
BshFI GGCC 2 cut(s) 150, 537
BsiHKAI GWGCWC 1 cut(s) 309
BsiHKCI CYCGRG 1 cut(s) 461
BsiSI CCGG 3 cut(s) 151, 384, 462
BslFI GGGAC 2 cut(s) 141, 526
BslI CCNNNNNNNGG 2 cut(s) 189, 529
BsmAI GTCTC 1 cut(s) 209
BsmFI GGGAC 2 cut(s) 141, 526
BsmI GAATGC 1 cut(s) 391
BsnI GGCC 2 cut(s) 150, 537
BsoBI CYCGRG 1 cut(s) 461
Bsp1286I GDGCHC 2 cut(s) 195, 309
Bsp143I GATC 4 cut(s) 64, 206, 325, 418
Bsp1720I GCTNAGC 1 cut(s) 583
BspACI CCGC 1 cut(s) 199
BspANI GGCC 2 cut(s) 150, 537
BspCNI CTCAG 2 cut(s) 115, 415
BspLI GGNNCC 2 cut(s) 420, 521
BspMI ACCTGC 1 cut(s) 506
BspPI GGATC 3 cut(s) 72, 413, 426
BspQI GCTCTTC 1 cut(s) 237
BsrFI RCCGGY 1 cut(s) 150
BsrI ACTGG 2 cut(s) 267, 534
BssAI RCCGGY 1 cut(s) 150
BssECI CCNNGG 1 cut(s) 461
BssMI GATC 4 cut(s) 64, 206, 325, 418
Bst2UI CCWGG 1 cut(s) 524
Bst4CI ACNGT 1 cut(s) 188
Bst6I CTCTTC 2 cut(s) 237, 423
BstC8I GCNNGC 3 cut(s) 344, 589, 593
BstDEI CTNAG 3 cut(s) 102, 402, 583
BstKTI GATC 4 cut(s) 67, 209, 328, 421
BstMAI GTCTC 1 cut(s) 209
BstMBI GATC 4 cut(s) 64, 206, 325, 418
BstMWI GCNNNNNNNGC 2 cut(s) 291, 588
BstNI CCWGG 1 cut(s) 524
BstNSI RCATGY 1 cut(s) 346
BstPAI GACNNNNGTC 1 cut(s) 518
BstSCI CCNGG 4 cut(s) 383, 460, 461, 522
BstSLI GKGCMC 1 cut(s) 195
BstV1I GCAGC 1 cut(s) 375
BstX2I RGATCY 1 cut(s) 418
BstXI CCANNNNNNTGG 1 cut(s) 232
BstYI RGATCY 1 cut(s) 418
BsuI GTATCC 1 cut(s) 549
BsuRI GGCC 2 cut(s) 150, 537
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 2 cut(s) 193, 309
BveI ACCTGC 1 cut(s) 506
Cac8I GCNNGC 3 cut(s) 344, 589, 593
CaiI CAGNNNCTG 1 cut(s) 498
Cfr10I RCCGGY 1 cut(s) 150
Cfr13I GGNCC 1 cut(s) 520
Cfr9I CCCGGG 1 cut(s) 461
Csp6I GTAC 1 cut(s) 527
CviAII CATG 5 cut(s) 166, 329, 343, 441, 572
CviJI RGCY 8 cut(s) 53, 106, 150, 247, 294, 388, 406, 537
CviKI_1 RGCY 8 cut(s) 53, 106, 150, 247, 294, 388, 406, 537
CviQI GTAC 1 cut(s) 527
DdeI CTNAG 3 cut(s) 102, 402, 583
DpnI GATC 4 cut(s) 66, 208, 327, 420
DpnII GATC 4 cut(s) 64, 206, 325, 418
EaeI YGGCCR 2 cut(s) 148, 535
Eam1104I CTCTTC 2 cut(s) 237, 423
EarI CTCTTC 2 cut(s) 237, 423
Eco47I GGWCC 1 cut(s) 520
Eco57I CTGAAG 1 cut(s) 447
Eco88I CYCGRG 1 cut(s) 461
EcoO109I RGGNCCY 1 cut(s) 520
EcoRII CCWGG 1 cut(s) 522
EcoT22I ATGCAT 1 cut(s) 277
FaeI CATG 5 cut(s) 169, 332, 346, 444, 575
FaiI YATR 9 cut(s) 39, 147, 167, 222, 330, 344, 442, 573, 610
FaqI GGGAC 2 cut(s) 141, 526
FatI CATG 5 cut(s) 165, 328, 342, 440, 571
FbaI TGATCA 1 cut(s) 325
Fnu4HI GCNGC 1 cut(s) 389
Fsp4HI GCNGC 1 cut(s) 389
FspBI CTAG 1 cut(s) 318
GluI GCNGC 1 cut(s) 389
GsuI CTGGAG 1 cut(s) 284
HaeIII GGCC 2 cut(s) 150, 537
HapII CCGG 3 cut(s) 151, 384, 462
Hin1II CATG 5 cut(s) 169, 332, 346, 444, 575
HinfI GANTC 2 cut(s) 160, 599
HpaII CCGG 3 cut(s) 151, 384, 462
HphI GGTGA 2 cut(s) 224, 557
Hpy166II GTNNAC 1 cut(s) 176
Hpy188I TCNGA 3 cut(s) 427, 509, 604
Hpy188III TCNNGA 1 cut(s) 29
Hpy8I GTNNAC 1 cut(s) 176
HpyAV CCTTC 4 cut(s) 107, 348, 408, 432
HpyCH4III ACNGT 1 cut(s) 188
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 5 cut(s) 92, 275, 302, 391, 571
HpyF10VI GCNNNNNNNGC 2 cut(s) 291, 588
HpyF3I CTNAG 3 cut(s) 102, 402, 583
HpySE526I ACGT 1 cut(s) 456
Hsp92II CATG 5 cut(s) 169, 332, 346, 444, 575
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 4 cut(s) 64, 206, 325, 418
LguI GCTCTTC 1 cut(s) 237
LmnI GCTCC 1 cut(s) 312
Lsp1109I GCAGC 1 cut(s) 375
LweI GCATC 1 cut(s) 101
MaeI CTAG 1 cut(s) 318
MaeII ACGT 1 cut(s) 456
MaeIII GTNAC 1 cut(s) 488
MalI GATC 4 cut(s) 66, 208, 327, 420
MboI GATC 4 cut(s) 64, 206, 325, 418
MboII GAAGA 3 cut(s) 248, 254, 440
MfeI CAATTG 1 cut(s) 378
MflI RGATCY 1 cut(s) 418
MhlI GDGCHC 2 cut(s) 195, 309
MlsI TGGCCA 1 cut(s) 537
MluCI AATT 3 cut(s) 378, 447, 476
MluNI TGGCCA 1 cut(s) 537
MmeI TCCRAC 1 cut(s) 487
MnlI CCTC 8 cut(s) 111, 249, 258, 321, 324, 422, 503, 541
Mox20I TGGCCA 1 cut(s) 537
Mph1103I ATGCAT 1 cut(s) 277
MscI TGGCCA 1 cut(s) 537
MseI TTAA 2 cut(s) 203, 561
MslI CAYNNNNRTG 2 cut(s) 500, 543
Msp20I TGGCCA 1 cut(s) 537
MspI CCGG 3 cut(s) 151, 384, 462
MspR9I CCNGG 4 cut(s) 385, 462, 463, 524
MunI CAATTG 1 cut(s) 378
Mva1269I GAATGC 1 cut(s) 391
MvaI CCWGG 1 cut(s) 524
MwoI GCNNNNNNNGC 2 cut(s) 291, 588
NciI CCSGG 3 cut(s) 385, 462, 463
NdeII GATC 4 cut(s) 64, 206, 325, 418
NlaIII CATG 5 cut(s) 169, 332, 346, 444, 575
NlaIV GGNNCC 2 cut(s) 420, 521
NmuCI GTSAC 1 cut(s) 488
NsiI ATGCAT 1 cut(s) 277
NspI RCATGY 1 cut(s) 346
OliI CACNNNNGTG 2 cut(s) 500, 543
PaeI GCATGC 1 cut(s) 346
PaqCI CACCTGC 1 cut(s) 506
PciSI GCTCTTC 1 cut(s) 237
PctI GAATGC 1 cut(s) 391
PfeI GAWTC 2 cut(s) 160, 599
PkrI GCNGC 1 cut(s) 390
PpuMI RGGWCCY 1 cut(s) 520
PshAI GACNNNNGTC 1 cut(s) 518
Psp1406I AACGTT 1 cut(s) 456
Psp5II RGGWCCY 1 cut(s) 520
Psp6I CCWGG 1 cut(s) 522
PspGI CCWGG 1 cut(s) 522
PspN4I GGNNCC 2 cut(s) 420, 521
PspPI GGNCC 1 cut(s) 520
PspPPI RGGWCCY 1 cut(s) 520
PstNI CAGNNNCTG 1 cut(s) 498
PsuI RGATCY 1 cut(s) 418
RsaI GTAC 1 cut(s) 528
RsaNI GTAC 1 cut(s) 527
RseI CAYNNNNRTG 2 cut(s) 500, 543
SapI GCTCTTC 1 cut(s) 237
SaqAI TTAA 2 cut(s) 203, 561
SatI GCNGC 1 cut(s) 389
Sau3AI GATC 4 cut(s) 64, 206, 325, 418
Sau96I GGNCC 1 cut(s) 520
ScrFI CCNGG 4 cut(s) 385, 462, 463, 524
SduI GDGCHC 2 cut(s) 195, 309
SfaNI GCATC 1 cut(s) 101
SinI GGWCC 1 cut(s) 520
SmaI CCCGGG 1 cut(s) 463
SmiMI CAYNNNNRTG 2 cut(s) 500, 543
SphI GCATGC 1 cut(s) 346
Sse9I AATT 3 cut(s) 378, 447, 476
SsiI CCGC 1 cut(s) 199
SspMI CTAG 1 cut(s) 318
StyD4I CCNGG 4 cut(s) 383, 460, 461, 522
TaaI ACNGT 1 cut(s) 188
TaiI ACGT 1 cut(s) 459
TasI AATT 3 cut(s) 378, 447, 476
TfiI GAWTC 2 cut(s) 160, 599
Tru1I TTAA 2 cut(s) 203, 561
Tru9I TTAA 2 cut(s) 203, 561
TscAI CASTG 2 cut(s) 193, 309
TseFI GTSAC 1 cut(s) 488
TseI GCWGC 1 cut(s) 388
Tsp45I GTSAC 1 cut(s) 488
TspDTI ATGAA 2 cut(s) 67, 255
TspMI CCCGGG 1 cut(s) 461
TspRI CASTG 2 cut(s) 193, 309
VpaK11BI GGWCC 1 cut(s) 520
XapI RAATTY 2 cut(s) 447, 476
XceI RCATGY 1 cut(s) 346
XmaI CCCGGG 1 cut(s) 461
XspI CTAG 1 cut(s) 318
Zsp2I ATGCAT 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.