RchiOBHm_Chr2g0132041

Domain of unknown function (DUF3337)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0022644
Physical Location & Seq
Reverse (-)
48434691 .. 48436663
1973 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 402 bp
ATGTTCCAATCTCTGCTTCTTGATTCTACTGGCAGGTTTTGCTTATCAGGATCTTCTGATTCAATGATTAGACTATGGGATCTTGGTCAGCAAAGATGCGTGCATTCTTATGCGGTGCACACAGATTCTGTTTGGGCACTTGCCAGTACGCCAACGTTTAGTCATGTATATAGTGGTGGGAGAGACCTTTTTTTGTACTTGACAGACTTGACCACAAGAGAGAGTCTTTTGCTTTGCCAAGGAGAACACCCCATTCTGCAGTTGGCTCTAGATGATGATAGCATATGGGTTGCAACAACAGATTCTTCAGTTAATAGATGGCCTGCTGAGGGGCGCAATCCTCAGAAAATTATCCAAAGAGGAGCGGTATGGGGATTACTGAAGCATTGCGGTTGCAGGTAG

Protein Analysis

133

Amino Acids

14.9

Weight (kDa)

6.16

Isoelectric Point (pI)

35.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 4 - 117 3.5e-06 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 5 - 89 3.4e-09 THOC3 beta-propeller domain
WD40_Prp19 PF24814 6 - 82 1.1e-09 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 9 - 107 6.9e-06 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 10 - 62 1.9e-09 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 11 - 100 1.1e-08 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0022644)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0132041
rosa_multiflora Rmu_sc0002737.1_g000010
rosa_roxburghii Rroxscaffold_4G00325900
rosa_samantha Rh4DG143500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 24, 387
AccBSI CCGCTC 1 cut(s) 365
AciI CCGC 3 cut(s) 113, 365, 390
AclI AACGTT 1 cut(s) 155
AclWI GGATC 2 cut(s) 58, 87
AcuI CTGAAG 2 cut(s) 291, 401
AfaI GTAC 2 cut(s) 148, 197
AfiI CCNNNNNNNGG 1 cut(s) 329
AgsI TTSAA 1 cut(s) 63
AjuI GAANNNNNNNTTGG 1 cut(s) 32
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 1 cut(s) 177
Alw44I GTGCAC 1 cut(s) 116
AlwI GGATC 2 cut(s) 58, 87
AlwNI CAGNNNCTG 1 cut(s) 128
AoxI GGCC 1 cut(s) 320
ApaLI GTGCAC 1 cut(s) 116
AspLEI GCGC 1 cut(s) 336
BaeGI GKGCMC 2 cut(s) 120, 139
Bbv12I GWGCWC 1 cut(s) 120
BbvCI CCTCAGC 1 cut(s) 327
BccI CCATC 1 cut(s) 312
BcoDI GTCTC 1 cut(s) 177
BfaI CTAG 1 cut(s) 269
BfmI CTRYAG 1 cut(s) 257
BfuAI ACCTGC 2 cut(s) 24, 387
BmsI GCATC 1 cut(s) 86
Bpu10I CCTNAGC 1 cut(s) 327
BsaI GGTCTC 1 cut(s) 177
BsaJI CCNNGG 1 cut(s) 238
Bsc4I CCNNNNNNNGG 1 cut(s) 329
Bse1I ACTGG 2 cut(s) 34, 144
Bse3DI GCAATG 1 cut(s) 385
BseDI CCNNGG 1 cut(s) 238
BseLI CCNNNNNNNGG 1 cut(s) 329
BseMI GCAATG 1 cut(s) 385
BseMII CTCAG 2 cut(s) 318, 356
BseNI ACTGG 2 cut(s) 34, 144
BseRI GAGGAG 1 cut(s) 375
BseSI GKGCMC 2 cut(s) 120, 139
BshFI GGCC 1 cut(s) 322
BsiHKAI GWGCWC 1 cut(s) 120
BslI CCNNNNNNNGG 1 cut(s) 329
BsmAI GTCTC 1 cut(s) 177
BsmI GAATGC 1 cut(s) 103
BsnI GGCC 1 cut(s) 322
Bso31I GGTCTC 1 cut(s) 177
Bsp1286I GDGCHC 2 cut(s) 120, 139
Bsp143I GATC 2 cut(s) 50, 79
BspACI CCGC 3 cut(s) 113, 365, 390
BspANI GGCC 1 cut(s) 322
BspCNI CTCAG 2 cut(s) 319, 355
BspMAI CTGCAG 1 cut(s) 261
BspMI ACCTGC 2 cut(s) 24, 387
BspPI GGATC 2 cut(s) 58, 87
BspTNI GGTCTC 1 cut(s) 177
BsrBI CCGCTC 1 cut(s) 365
BsrDI GCAATG 1 cut(s) 385
BsrI ACTGG 2 cut(s) 34, 144
BssECI CCNNGG 1 cut(s) 238
BssMI GATC 2 cut(s) 50, 79
BssT1I CCWWGG 1 cut(s) 238
BstAPI GCANNNNNTGC 1 cut(s) 39
BstC8I GCNNGC 2 cut(s) 101, 324
BstDEI CTNAG 2 cut(s) 327, 342
BstHHI GCGC 1 cut(s) 336
BstKTI GATC 2 cut(s) 53, 82
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 2 cut(s) 50, 79
BstMWI GCNNNNNNNGC 1 cut(s) 39
BstSFI CTRYAG 1 cut(s) 257
BstSLI GKGCMC 2 cut(s) 120, 139
BstX2I RGATCY 2 cut(s) 50, 79
BstYI RGATCY 2 cut(s) 50, 79
BsuRI GGCC 1 cut(s) 322
BveI ACCTGC 2 cut(s) 24, 387
Cac8I GCNNGC 2 cut(s) 101, 324
CaiI CAGNNNCTG 1 cut(s) 128
CfoI GCGC 1 cut(s) 336
Csp6I GTAC 2 cut(s) 147, 196
CviAII CATG 1 cut(s) 164
CviJI RGCY 2 cut(s) 266, 322
CviKI_1 RGCY 2 cut(s) 266, 322
CviQI GTAC 2 cut(s) 147, 196
DdeI CTNAG 2 cut(s) 327, 342
DpnI GATC 2 cut(s) 52, 81
DpnII GATC 2 cut(s) 50, 79
Eco130I CCWWGG 1 cut(s) 238
Eco31I GGTCTC 1 cut(s) 177
Eco57I CTGAAG 2 cut(s) 291, 401
EcoT14I CCWWGG 1 cut(s) 238
ErhI CCWWGG 1 cut(s) 238
FaeI CATG 1 cut(s) 167
FaiI YATR 8 cut(s) 76, 111, 165, 169, 171, 284, 286, 370
FatI CATG 1 cut(s) 163
FauNDI CATATG 1 cut(s) 284
FspBI CTAG 1 cut(s) 269
GlaI GCGC 1 cut(s) 335
HaeIII GGCC 1 cut(s) 322
HhaI GCGC 1 cut(s) 336
Hin1II CATG 1 cut(s) 167
Hin6I GCGC 1 cut(s) 334
HinP1I GCGC 1 cut(s) 334
HinfI GANTC 5 cut(s) 23, 59, 125, 223, 302
Hpy166II GTNNAC 1 cut(s) 118
Hpy188I TCNGA 2 cut(s) 58, 345
Hpy188III TCNNGA 3 cut(s) 20, 48, 269
Hpy8I GTNNAC 1 cut(s) 118
HpyCH4IV ACGT 1 cut(s) 155
HpyCH4V TGCA 5 cut(s) 103, 118, 259, 293, 396
HpyF10VI GCNNNNNNNGC 1 cut(s) 39
HpyF3I CTNAG 2 cut(s) 327, 342
HpySE526I ACGT 1 cut(s) 155
Hsp92II CATG 1 cut(s) 167
HspAI GCGC 1 cut(s) 334
Kzo9I GATC 2 cut(s) 50, 79
LmnI GCTCC 1 cut(s) 362
LpnPI CCDG 6 cut(s) 15, 19, 33, 157, 336, 382
LweI GCATC 1 cut(s) 86
MaeI CTAG 1 cut(s) 269
MaeII ACGT 1 cut(s) 155
MalI GATC 2 cut(s) 52, 81
MbiI CCGCTC 1 cut(s) 365
MboI GATC 2 cut(s) 50, 79
MboII GAAGA 2 cut(s) 45, 297
MflI RGATCY 2 cut(s) 50, 79
MhlI GDGCHC 2 cut(s) 120, 139
MluCI AATT 1 cut(s) 348
MlyI GAGTC 1 cut(s) 232
MnlI CCTC 3 cut(s) 322, 351, 353
MseI TTAA 1 cut(s) 312
MslI CAYNNNNRTG 1 cut(s) 108
Mva1269I GAATGC 1 cut(s) 103
MwoI GCNNNNNNNGC 1 cut(s) 39
NdeI CATATG 1 cut(s) 284
NdeII GATC 2 cut(s) 50, 79
NlaIII CATG 1 cut(s) 167
PctI GAATGC 1 cut(s) 103
PfeI GAWTC 4 cut(s) 23, 59, 125, 302
PleI GAGTC 1 cut(s) 231
PpsI GAGTC 1 cut(s) 231
Psp1406I AACGTT 1 cut(s) 155
PstI CTGCAG 1 cut(s) 261
PstNI CAGNNNCTG 1 cut(s) 128
PsuI RGATCY 2 cut(s) 50, 79
RsaI GTAC 2 cut(s) 148, 197
RsaNI GTAC 2 cut(s) 147, 196
RseI CAYNNNNRTG 1 cut(s) 108
SaqAI TTAA 1 cut(s) 312
Sau3AI GATC 2 cut(s) 50, 79
SchI GAGTC 1 cut(s) 232
SduI GDGCHC 2 cut(s) 120, 139
SetI ASST 4 cut(s) 38, 158, 189, 401
SfaNI GCATC 1 cut(s) 86
SfcI CTRYAG 1 cut(s) 257
SmiMI CAYNNNNRTG 1 cut(s) 108
Sse9I AATT 1 cut(s) 348
SsiI CCGC 3 cut(s) 113, 365, 390
SspMI CTAG 1 cut(s) 269
StyI CCWWGG 1 cut(s) 238
TaiI ACGT 1 cut(s) 158
TasI AATT 1 cut(s) 348
TatI WGTACW 1 cut(s) 195
TfiI GAWTC 4 cut(s) 23, 59, 125, 302
Tru1I TTAA 1 cut(s) 312
Tru9I TTAA 1 cut(s) 312
VneI GTGCAC 1 cut(s) 116
XbaI TCTAGA 1 cut(s) 268
XcmI CCANNNNNNNNNTGG 1 cut(s) 259
XspI CTAG 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.