Rroxscaffold_4G00325900

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0022644
Physical Location & Seq
Reverse (-)
57923229 .. 57924328
1100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00325900.1

Sequence Viewer

Length: 402 bp
ATGCATGATAGTGGATCACTTCTTGTCTCGGTGGAACATATGCATGATAATGGATATGTTCCAATCTCTCGCTTCTTGATTCTACTTGGCAGACTATGGGATCTTGGTCAGCAAAGATGCGTGCATTCTTATGCGGTGCACACAGATTCTGTTTGGGCACTTGCCGATACGCCAACGTTTAGTCATGTATATAGTGGTGGGAGAGACCTTTCTTTGTACTTGACAGACTTGACCACAAGAGAGAGTCTTTTGCTTTGCCAAGGAGAACACCCCATTCTGCAGTTGGCTCTAGATGATGATAGCATATGGGTTGCAACAACAGATTCTTCGGTTAATAGATGGCCTGCGAGGGCGCAATCCTCGAGAAAATTATCCAAAGAGGAGCGGTATGGGGATTACTGA

Protein Analysis

133

Amino Acids

15.06

Weight (kDa)

5.51

Isoelectric Point (pI)

45.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 31 - 121 4.8e-06 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022644)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0132041
rosa_multiflora Rmu_sc0002737.1_g000010
rosa_roxburghii Rroxscaffold_4G00325900
rosa_samantha Rh4DG143500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 385
AciI CCGC 2 cut(s) 134, 385
AclI AACGTT 1 cut(s) 176
AclWI GGATC 2 cut(s) 22, 108
AfaI GTAC 1 cut(s) 218
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 2 cut(s) 31, 198
Alw44I GTGCAC 1 cut(s) 137
AlwI GGATC 2 cut(s) 22, 108
AlwNI CAGNNNCTG 1 cut(s) 149
Ama87I CYCGRG 1 cut(s) 361
AoxI GGCC 1 cut(s) 341
ApaLI GTGCAC 1 cut(s) 137
AspLEI GCGC 1 cut(s) 355
AvaI CYCGRG 1 cut(s) 361
BaeGI GKGCMC 2 cut(s) 141, 160
Bbv12I GWGCWC 1 cut(s) 141
BccI CCATC 1 cut(s) 333
BcoDI GTCTC 2 cut(s) 31, 198
BfaI CTAG 1 cut(s) 290
BfmI CTRYAG 1 cut(s) 278
BmeT110I CYCGRG 1 cut(s) 361
BmsI GCATC 1 cut(s) 107
BsaI GGTCTC 1 cut(s) 198
BsaJI CCNNGG 1 cut(s) 259
BseDI CCNNGG 1 cut(s) 259
BseRI GAGGAG 1 cut(s) 395
BseSI GKGCMC 2 cut(s) 141, 160
BshFI GGCC 1 cut(s) 343
BsiHKAI GWGCWC 1 cut(s) 141
BsiHKCI CYCGRG 1 cut(s) 361
BsmAI GTCTC 2 cut(s) 31, 198
BsmI GAATGC 1 cut(s) 124
BsnI GGCC 1 cut(s) 343
Bso31I GGTCTC 1 cut(s) 198
BsoBI CYCGRG 1 cut(s) 361
Bsp1286I GDGCHC 2 cut(s) 141, 160
Bsp143I GATC 2 cut(s) 14, 100
BspACI CCGC 2 cut(s) 134, 385
BspANI GGCC 1 cut(s) 343
BspMAI CTGCAG 1 cut(s) 282
BspPI GGATC 2 cut(s) 22, 108
BspTNI GGTCTC 1 cut(s) 198
BsrBI CCGCTC 1 cut(s) 385
BssECI CCNNGG 1 cut(s) 259
BssMI GATC 2 cut(s) 14, 100
BssT1I CCWWGG 1 cut(s) 259
BstC8I GCNNGC 2 cut(s) 122, 345
BstHHI GCGC 1 cut(s) 355
BstKTI GATC 2 cut(s) 17, 103
BstMAI GTCTC 2 cut(s) 31, 198
BstMBI GATC 2 cut(s) 14, 100
BstSFI CTRYAG 1 cut(s) 278
BstSLI GKGCMC 2 cut(s) 141, 160
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BsuRI GGCC 1 cut(s) 343
Cac8I GCNNGC 2 cut(s) 122, 345
CaiI CAGNNNCTG 1 cut(s) 149
CfoI GCGC 1 cut(s) 355
Csp6I GTAC 1 cut(s) 217
CviAII CATG 3 cut(s) 5, 44, 185
CviJI RGCY 2 cut(s) 287, 343
CviKI_1 RGCY 2 cut(s) 287, 343
CviQI GTAC 1 cut(s) 217
DpnI GATC 2 cut(s) 16, 102
DpnII GATC 2 cut(s) 14, 100
Eco130I CCWWGG 1 cut(s) 259
Eco31I GGTCTC 1 cut(s) 198
Eco88I CYCGRG 1 cut(s) 361
EcoT14I CCWWGG 1 cut(s) 259
EcoT22I ATGCAT 2 cut(s) 6, 45
ErhI CCWWGG 1 cut(s) 259
FaeI CATG 3 cut(s) 8, 47, 188
FatI CATG 3 cut(s) 4, 43, 184
FauNDI CATATG 2 cut(s) 39, 305
FspBI CTAG 1 cut(s) 290
GlaI GCGC 1 cut(s) 354
HaeIII GGCC 1 cut(s) 343
HhaI GCGC 1 cut(s) 355
Hin1II CATG 3 cut(s) 8, 47, 188
Hin6I GCGC 1 cut(s) 353
HinP1I GCGC 1 cut(s) 353
HinfI GANTC 4 cut(s) 79, 146, 244, 323
Hpy166II GTNNAC 1 cut(s) 139
Hpy188III TCNNGA 3 cut(s) 76, 290, 363
Hpy8I GTNNAC 1 cut(s) 139
HpyCH4IV ACGT 1 cut(s) 176
HpyCH4V TGCA 6 cut(s) 4, 43, 124, 139, 280, 314
HpySE526I ACGT 1 cut(s) 176
Hsp92II CATG 3 cut(s) 8, 47, 188
HspAI GCGC 1 cut(s) 353
Kzo9I GATC 2 cut(s) 14, 100
LmnI GCTCC 1 cut(s) 382
LpnPI CCDG 1 cut(s) 357
LweI GCATC 1 cut(s) 107
MaeI CTAG 1 cut(s) 290
MaeII ACGT 1 cut(s) 176
MalI GATC 2 cut(s) 16, 102
MbiI CCGCTC 1 cut(s) 385
MboI GATC 2 cut(s) 14, 100
MboII GAAGA 1 cut(s) 318
MflI RGATCY 1 cut(s) 100
MhlI GDGCHC 2 cut(s) 141, 160
MluCI AATT 1 cut(s) 368
MlyI GAGTC 1 cut(s) 253
MnlI CCTC 3 cut(s) 342, 370, 373
Mph1103I ATGCAT 2 cut(s) 6, 45
MseI TTAA 1 cut(s) 333
MslI CAYNNNNRTG 4 cut(s) 9, 42, 48, 129
Mva1269I GAATGC 1 cut(s) 124
NdeI CATATG 2 cut(s) 39, 305
NdeII GATC 2 cut(s) 14, 100
NlaIII CATG 3 cut(s) 8, 47, 188
NsiI ATGCAT 2 cut(s) 6, 45
PaeR7I CTCGAG 1 cut(s) 361
PctI GAATGC 1 cut(s) 124
PfeI GAWTC 3 cut(s) 79, 146, 323
PleI GAGTC 1 cut(s) 252
PpsI GAGTC 1 cut(s) 252
Psp1406I AACGTT 1 cut(s) 176
PstI CTGCAG 1 cut(s) 282
PstNI CAGNNNCTG 1 cut(s) 149
PsuI RGATCY 1 cut(s) 100
RsaI GTAC 1 cut(s) 218
RsaNI GTAC 1 cut(s) 217
RseI CAYNNNNRTG 4 cut(s) 9, 42, 48, 129
SaqAI TTAA 1 cut(s) 333
Sau3AI GATC 2 cut(s) 14, 100
SchI GAGTC 1 cut(s) 253
SduI GDGCHC 2 cut(s) 141, 160
SetI ASST 2 cut(s) 179, 210
SfaNI GCATC 1 cut(s) 107
SfcI CTRYAG 1 cut(s) 278
Sfr274I CTCGAG 1 cut(s) 361
SlaI CTCGAG 1 cut(s) 361
SmiMI CAYNNNNRTG 4 cut(s) 9, 42, 48, 129
SmlI CTYRAG 1 cut(s) 361
SmoI CTYRAG 1 cut(s) 361
Sse9I AATT 1 cut(s) 368
SsiI CCGC 2 cut(s) 134, 385
SspMI CTAG 1 cut(s) 290
StyI CCWWGG 1 cut(s) 259
TaiI ACGT 1 cut(s) 179
TaqI TCGA 1 cut(s) 362
TasI AATT 1 cut(s) 368
TatI WGTACW 1 cut(s) 216
TfiI GAWTC 3 cut(s) 79, 146, 323
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
VneI GTGCAC 1 cut(s) 137
XbaI TCTAGA 1 cut(s) 289
XcmI CCANNNNNNNNNTGG 1 cut(s) 280
XhoI CTCGAG 1 cut(s) 361
XspI CTAG 1 cut(s) 290
Zsp2I ATGCAT 2 cut(s) 6, 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.