Rh4DG143500

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0022644
Physical Location & Seq
Reverse (-)
28375401 .. 28376472
1072 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG143500.1

Sequence Viewer

Length: 408 bp
ATGGATATGTTCCAATCTCTGCTTCTTGATTCTACTGGCAGGTTTTGCTTATCAGGATCTTCTTATTCAATGATTAGACTATGGGATCTTGGTCAGCAAAGATGCGTGCATTCTTATGCGGTGCACACAGATTCTGTTTGGGCACTTGCCAGTACGCCAACATTTAGTCATGTATATAGTGGTGGGAGAGACCTTTCTTTGTACTTGACAGACTTGACCACAAGAGAGAGTCTTTTGCTTTGCCAAGGAGAACACCCCATTCTGCAGTTGGCTCTAGATGATGATAGCATATGGGTTGCAACAACAGATTCTTCAGTTAATAGATGGCCTGCTGAGGGGCGCAATCCTCAGAAAATTATCCAAAGAGGAGCGGTATGGGGATTACTGAAGCATTGCGGTTGCAGGTAG

Protein Analysis

135

Amino Acids

15.13

Weight (kDa)

6.16

Isoelectric Point (pI)

33.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 3 - 120 1.8e-06 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 8 - 84 3.3e-08 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 10 - 110 4.9e-09 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 10 - 100 1.2e-07 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 13 - 100 2.5e-07 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0022644)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0132041
rosa_multiflora Rmu_sc0002737.1_g000010
rosa_roxburghii Rroxscaffold_4G00325900
rosa_samantha Rh4DG143500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 30, 393
AccBSI CCGCTC 1 cut(s) 371
AciI CCGC 3 cut(s) 119, 371, 396
AclWI GGATC 2 cut(s) 64, 93
AcuI CTGAAG 2 cut(s) 297, 407
AfaI GTAC 2 cut(s) 154, 203
AfiI CCNNNNNNNGG 1 cut(s) 335
AgsI TTSAA 1 cut(s) 69
AjuI GAANNNNNNNTTGG 1 cut(s) 38
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 1 cut(s) 183
Alw44I GTGCAC 1 cut(s) 122
AlwI GGATC 2 cut(s) 64, 93
AlwNI CAGNNNCTG 1 cut(s) 134
AoxI GGCC 1 cut(s) 326
ApaLI GTGCAC 1 cut(s) 122
AspLEI GCGC 1 cut(s) 342
BaeGI GKGCMC 2 cut(s) 126, 145
Bbv12I GWGCWC 1 cut(s) 126
BbvCI CCTCAGC 1 cut(s) 333
BccI CCATC 1 cut(s) 318
BcoDI GTCTC 1 cut(s) 183
BfaI CTAG 1 cut(s) 275
BfmI CTRYAG 1 cut(s) 263
BfuAI ACCTGC 2 cut(s) 30, 393
BmsI GCATC 1 cut(s) 92
Bpu10I CCTNAGC 1 cut(s) 333
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 244
Bsc4I CCNNNNNNNGG 1 cut(s) 335
Bse1I ACTGG 2 cut(s) 40, 150
Bse3DI GCAATG 1 cut(s) 391
BseDI CCNNGG 1 cut(s) 244
BseLI CCNNNNNNNGG 1 cut(s) 335
BseMI GCAATG 1 cut(s) 391
BseMII CTCAG 2 cut(s) 324, 362
BseNI ACTGG 2 cut(s) 40, 150
BseRI GAGGAG 1 cut(s) 381
BseSI GKGCMC 2 cut(s) 126, 145
BshFI GGCC 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 126
BslI CCNNNNNNNGG 1 cut(s) 335
BsmAI GTCTC 1 cut(s) 183
BsmI GAATGC 1 cut(s) 109
BsnI GGCC 1 cut(s) 328
Bso31I GGTCTC 1 cut(s) 183
Bsp1286I GDGCHC 2 cut(s) 126, 145
Bsp143I GATC 2 cut(s) 56, 85
BspACI CCGC 3 cut(s) 119, 371, 396
BspANI GGCC 1 cut(s) 328
BspCNI CTCAG 2 cut(s) 325, 361
BspMAI CTGCAG 1 cut(s) 267
BspMI ACCTGC 2 cut(s) 30, 393
BspPI GGATC 2 cut(s) 64, 93
BspTNI GGTCTC 1 cut(s) 183
BsrBI CCGCTC 1 cut(s) 371
BsrDI GCAATG 1 cut(s) 391
BsrI ACTGG 2 cut(s) 40, 150
BssECI CCNNGG 1 cut(s) 244
BssMI GATC 2 cut(s) 56, 85
BssT1I CCWWGG 1 cut(s) 244
BstAPI GCANNNNNTGC 1 cut(s) 45
BstC8I GCNNGC 2 cut(s) 107, 330
BstDEI CTNAG 2 cut(s) 333, 348
BstHHI GCGC 1 cut(s) 342
BstKTI GATC 2 cut(s) 59, 88
BstMAI GTCTC 1 cut(s) 183
BstMBI GATC 2 cut(s) 56, 85
BstMWI GCNNNNNNNGC 1 cut(s) 45
BstSFI CTRYAG 1 cut(s) 263
BstSLI GKGCMC 2 cut(s) 126, 145
BstX2I RGATCY 2 cut(s) 56, 85
BstYI RGATCY 2 cut(s) 56, 85
BsuRI GGCC 1 cut(s) 328
BveI ACCTGC 2 cut(s) 30, 393
Cac8I GCNNGC 2 cut(s) 107, 330
CaiI CAGNNNCTG 1 cut(s) 134
CfoI GCGC 1 cut(s) 342
Csp6I GTAC 2 cut(s) 153, 202
CviAII CATG 1 cut(s) 170
CviJI RGCY 2 cut(s) 272, 328
CviKI_1 RGCY 2 cut(s) 272, 328
CviQI GTAC 2 cut(s) 153, 202
DdeI CTNAG 2 cut(s) 333, 348
DpnI GATC 2 cut(s) 58, 87
DpnII GATC 2 cut(s) 56, 85
Eco130I CCWWGG 1 cut(s) 244
Eco31I GGTCTC 1 cut(s) 183
Eco57I CTGAAG 2 cut(s) 297, 407
EcoT14I CCWWGG 1 cut(s) 244
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 1 cut(s) 173
FaiI YATR 9 cut(s) 8, 82, 117, 171, 175, 177, 290, 292, 376
FatI CATG 1 cut(s) 169
FauNDI CATATG 1 cut(s) 290
FspBI CTAG 1 cut(s) 275
GlaI GCGC 1 cut(s) 341
HaeIII GGCC 1 cut(s) 328
HhaI GCGC 1 cut(s) 342
Hin1II CATG 1 cut(s) 173
Hin6I GCGC 1 cut(s) 340
HinP1I GCGC 1 cut(s) 340
HinfI GANTC 4 cut(s) 29, 131, 229, 308
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 1 cut(s) 351
Hpy188III TCNNGA 3 cut(s) 26, 54, 275
Hpy8I GTNNAC 1 cut(s) 124
HpyCH4V TGCA 5 cut(s) 109, 124, 265, 299, 402
HpyF10VI GCNNNNNNNGC 1 cut(s) 45
HpyF3I CTNAG 2 cut(s) 333, 348
Hsp92II CATG 1 cut(s) 173
HspAI GCGC 1 cut(s) 340
Kzo9I GATC 2 cut(s) 56, 85
LmnI GCTCC 1 cut(s) 368
LpnPI CCDG 6 cut(s) 21, 25, 39, 163, 342, 388
LweI GCATC 1 cut(s) 92
MaeI CTAG 1 cut(s) 275
MalI GATC 2 cut(s) 58, 87
MbiI CCGCTC 1 cut(s) 371
MboI GATC 2 cut(s) 56, 85
MboII GAAGA 2 cut(s) 51, 303
MflI RGATCY 2 cut(s) 56, 85
MhlI GDGCHC 2 cut(s) 126, 145
MluCI AATT 1 cut(s) 354
MlyI GAGTC 1 cut(s) 238
MnlI CCTC 3 cut(s) 328, 357, 359
MseI TTAA 1 cut(s) 318
MslI CAYNNNNRTG 1 cut(s) 114
Mva1269I GAATGC 1 cut(s) 109
MwoI GCNNNNNNNGC 1 cut(s) 45
NdeI CATATG 1 cut(s) 290
NdeII GATC 2 cut(s) 56, 85
NlaIII CATG 1 cut(s) 173
PctI GAATGC 1 cut(s) 109
PfeI GAWTC 3 cut(s) 29, 131, 308
PleI GAGTC 1 cut(s) 237
PpsI GAGTC 1 cut(s) 237
PstI CTGCAG 1 cut(s) 267
PstNI CAGNNNCTG 1 cut(s) 134
PsuI RGATCY 2 cut(s) 56, 85
RsaI GTAC 2 cut(s) 154, 203
RsaNI GTAC 2 cut(s) 153, 202
RseI CAYNNNNRTG 1 cut(s) 114
SaqAI TTAA 1 cut(s) 318
Sau3AI GATC 2 cut(s) 56, 85
SchI GAGTC 1 cut(s) 238
SduI GDGCHC 2 cut(s) 126, 145
SetI ASST 3 cut(s) 44, 195, 407
SfaNI GCATC 1 cut(s) 92
SfcI CTRYAG 1 cut(s) 263
SmiMI CAYNNNNRTG 1 cut(s) 114
Sse9I AATT 1 cut(s) 354
SsiI CCGC 3 cut(s) 119, 371, 396
SspMI CTAG 1 cut(s) 275
StyI CCWWGG 1 cut(s) 244
TasI AATT 1 cut(s) 354
TatI WGTACW 1 cut(s) 201
TfiI GAWTC 3 cut(s) 29, 131, 308
Tru1I TTAA 1 cut(s) 318
Tru9I TTAA 1 cut(s) 318
VneI GTGCAC 1 cut(s) 122
XbaI TCTAGA 1 cut(s) 274
XcmI CCANNNNNNNNNTGG 1 cut(s) 265
XspI CTAG 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.